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| 1 | Preparation and activity of conjugate of monoclonal antibody HAb18 against hepatoma F( ab′ )_2 fragment and staphylococcal enterotoxin A显示文摘AIM To prepare the conjugate of staphylococcal enterotoxin A (SEA) protein which is a bacterial SAg and the F(ab')2 fragment of mAb HAbl8 against human hepatocellular carcinoma (HCC), and identify its activity in order to use SAg in the targeting therapy of HCC.METHODS MAb HAbl8 was extracted from the abdominal dropsy of Balb/ c mice, and was purified through chromatography column SP-40HR with Fast protein liquid chromatography (FPLC) system. The F(ab')2 fragment of mAb HAb18 was prepared by papainic digestion method. The conjugate of mAb HAb18 F(ab')2fragment and SEA was prepared with chemical conjugating reagent N-succinimidyl-3-( 2-pyridyldithio) propionate (SPDP) and purified through chromatography column Superose 12with FPLC system. The molecular mass and purity of each collected peak were identified with SDS-PAGE assay. The protein content was assayed by Lowry's method. The antibody activity of HAb18 F (ab')2 against HCC in the conjugate was identified by indirect immunocytochemical ABC method, and the activity of SEA in the conjugate to activate peripheral blood mononuclear cells (PBMC) was identified with MTT assay.RESULTS The lgG mAb HAb18 was extracted,and purified successfully. Immunocytochemical staining demonstrated that it reacted with most of HHCC cells of human HCC cell line. There were two peaks in the process of purification of the prepared HAb18 F(ab)2-SEA conjugate. SDS-PAGE assay demonstrated that the molecular mass of the first peak was about 130 ku, and the second peak was the mixture of about 45 ku and a little 100 ku proteins. The immunocytochemical staining was similar in HAb18 F (ab ')2-SEAconjugate and HAb18 F (ab ')2, i.e., thecytoplasm and/or cell membranes of most HHCC cells were positively stained. The MTT assay showed that the optical absorbance (A) value at 490 nm of HAb18 F (ab')2-SEA conjugate was 0.182 ± 0.012, that of negative control was 0.033± 0.009, and there was significant difference between them ( P < 0.05).CONCLUSION SPDP is a good proteinconjugating reagent and can be used in preparing protein conjugate. The conjugate of mAb HAb18F(ab')2 fragment and SEA protein was preparedsuccessfully in present study and can be used in the experimental study of HCC targeting therapy with the conjugate of SAg and anti-HCC mAbs or their fragments. | Lian Jun Yang Yan Fang Sui Zhi Nan Chen Department of Pathology, Fourth Military Medical University. Xi’an 710032, Shaanxi Province, China | 2001 | World Journal of Gastroenterology2001，7，2： | 20 |
| 2 | Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma显示文摘INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3fucosyltransfrease and a2, 3sialyctransferase.In adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells . | Xiao Wei Li~1 Yan Qing Ding~1 Jun Jie Cai~1 Shao Qing Yang~2 Lian Bing An~3 Dong Fang Qiao~3 ~1Department of Pathology,Nanfang Hospital of the First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,China ~3Department of Electronmicroscopy,First Military Medical University,Guangzhou 510515,Gangdong Province,ChinaDr.Xiao Wei Li graduated from the First Military Medical University with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 3 | A pure blue light emitting binaphthyl derivative:Synthesis and properties显示文摘有 3 和 3 个′位置上的分核的联萘衍生物被综合并且经由铃木描绘了偶合反应。在答案最大的排放被作为参考拿 9,10-diphenyl 蒽与 68% 的量子效率在 390 nm 定位,当它与从聚集状态导致固态膜的 104 nm 的 FWHM 被转移到 450 nm 时。玻璃化转变温度(Tg ) 和分解温度被测量分别地,由 DSC 和 TGA 是 184 和 447 ° C。不同于它的光致发光光谱,电镀物品发光光谱在大约 460 nm 和表演达到顶点相应于纯蓝排出物的 69 nm 的 FWHM。刺激电压,发光性和效率最大值是有并列的 CIE 颜色的 5 V, 2953 cd/m2 和 1.37 cd/A (0.16, 0.15 ) ,在 ITO/NPB (40 nm ) 的设备结构 /PY-BN-PY (15 nm )/BPhen (40 nm )/Mg :47 号元素银的化学符号。 | Qing Guo He Zeng Ze Chu Gang Tie Lei An Jun Qin Hong Zhen Lin Feng Lian Bai Jian Gong Cheng Yong Qiu | 2008 | Chinese Chemical Letters2008，19，4： | 4 |
| 4 | Progress of Jinping Underground laboratory for Nuclear Astrophysics(JUNA)显示文摘Jinping Underground laboratory for Nuclear Astrophysics(JUNA) will take the advantage of the ultra-low background of CJPL lab and high current accelerator based on an ECR source and a highly sensitive detector to directly study for the first time a number of crucial reactions occurring at their relevant stellar energies during the evolution of hydrostatic stars. In its first phase, JUNA aims at the direct measurements of^(25)Mg(p,γ)^(26)Al,^(19)F(p,α)^(16)O,^(13)C(α,n)^(16)O and ^(12)C(α,γ)^(16)O reactions. The experimental setup,which includes an accelerator system with high stability and high intensity, a detector system, and a shielding material with low background, will be established during the above research. The current progress of JUNA will be given. | WeiPing Liu ZhiHong Li JiangJun He XiaoDong Tang Gang Lian Zhu An JianJun Chang Han Chen QingHao Chen XiongJun Chen ZhiJun Chen BaoQun Cui XianChao Du ChangBo Fu Lin Gan Bing Guo GuoZhu He Alexander Heger SuQing Hou HanXiong Huang Ning Huang BaoLu Jia LiYang Jiang Shigeru Kubono JianMin Li KuoAng Li Tao Li YunJu Li Maria Lugaro XiaoBing Luo HongYi Ma ShaoBo Ma DongMing Mei YongZhong Qian JiuChang Qin Jie Ren YangPing Shen Jun Su LiangTing Sun WanPeng Tan Isao Tanihata Shuo Wang Peng Wang YouBao Wang Qi Wu ShiWei Xu ShengQuan Yan LiTao Yang Yao Yang XiangQing Yu Qian Yue Sheng Zeng HuanYu Zhang Hui Zhang LiYong Zhang NingTao Zhang QiWei Zhang Tao Zhang XiaoPeng Zhang XueZhen Zhang ZiMing Zhang Wei Zhao Zuo Zhao Chao Zhou | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，4： | 3 |
| 5 | Cardamine komarovii flower extract reduces lipopolysaccharide-induced acute lung injury by inhibiting MyD88/TRIF signaling pathways显示文摘In the present study, we investigated anti-inflammatory effect of Cardamine komarovii flower(CKF) on lipopolysaccharide(LPS)-induced acute lung injury(ALI). We determined the effect of CKF methanolic extracts on LPS-induced pro-inflammatory mediators NO and prostaglandin E2(PGE2), production of pro-inflammatory cytokines(IL-1β, TNF-α, and IL-6), and related protein expression levels of MyD88/TRIF signaling pathways in peritoneal macrophages(PMs). Nuclear translocation of NF-κB-p65 was analyzed by immunofluorescence. For the in vivo experiments, an ALI model was established to detect the number of inflammatory cells and inflammatory factors(IL-1β, TNF-α, and IL-6) in bronchoalveolar lavage fluid(BALF) of mice. The pathological damage in lung tissues was evaluated through H&E staining. Our results showed that CKF can decrease the production of inflammatory mediators, such as NO and PGE2, by inhibiting their synthesis-related enzymes i NOS and COX-2 in LPS-induced PMs. In addition, CKF can downregulate the mRNA levels of IL-1β, TNF-α, and IL-6 to inhibit the production of inflammatory factors. Mechanism studies indicated that CKF possesses a fine anti-inflammatory effect by regulating My D88/TRIF dependent signaling pathways. Immunocytochemistry staining showed that the CKF extract attenuates the LPS-induced translocation of NF-kB p65 subunit in the nucleus from the cytoplasm. In vivo experiments revealed that the number of inflammatory cells and IL-1β in BALF of mice decrease after CKF treatment. Histopathological observation of lung tissues showed that CKF can remarkably improve alveolar clearance and infiltration of interstitial and alveolar cells after LPS stimulation. In conclusion, our results suggest that CKF inhibits LPS-induced inflammatory response by inhibiting the MyD88/TRIF signaling pathways, thereby protecting mice from LPS-induced ALI. | CHEN Qi ZHANG Ke-Xin LI Tai-Yuan PIAO Xuan-Mei LIAN Mei-Lan AN Ren-Bo JIANG Jun | 2019 | Chinese Journal of Natural Medicines2019，17，6： | 2 |
| 6 | Improved biocompatibility of poly(vinylpyrrolidone)-graft-poly(2-dimethylaminoethyl methacrylate)/DNA complexes by coating with bovine serum albumin显示文摘牛的浆液白朊(BSA ) 被利用与二进制建筑群集合 poly (vinylpyrrolidone )-graft-poly(2-dimethylaminoethyl methacrylate )(PVP-g-PDMAEMA )/DNA 由 layer-by-layer 形成了静电的相互作用屏蔽建筑群的剩余表面正电荷。BSA 的涂层能减少二进制建筑群的希腊语的第六个字母潜力将近到没有防碍 DNA 的 electroneutrality 冷凝作用能力。表明的 BSA/PVP-g-PDMAEMA/DNA 的第三的建筑群在 HepG2 房间与二进制建筑群和也维持的高基因 transfection 效率相比降低 cytotoxicity。 | Biao Zhang Lian Dong Deng Jin Feng Xing Jun Yang An Jie Dong | 2012 | Chinese Chemical Letters2012，23，5： | 2 |
| 7 | Panda X-III: Searching for neutrinoless double beta decay with high pressure ^(136)Xe gas time projection chambers显示文摘Searching for the neutrinoless double beta decay(NLDBD)is now regarded as the topmost promising technique to explore the nature of neutrinos after the discovery of neutrino masses in oscillation experiments.Panda X-III(particle and astrophysical xenon experiment III)will search for the NLDBD of136Xe at the China Jin Ping Underground Laboratory(CJPL).In the first phase of the experiment,a high pressure gas Time Projection Chamber(TPC)will contain 200 kg,90%136Xe enriched gas operated at10 bar.Fine pitch micro-pattern gas detector(Microbulk Micromegas)will be used at both ends of the TPC for the charge readout with a cathode in the middle.Charge signals can be used to reconstruct the electron tracks of the NLDBD events and provide good energy and spatial resolution.The detector will be immersed in a large water tank to ensure~5 m of water shielding in all directions.The second phase,a ton-scale experiment,will consist of five TPCs in the same water tank,with improved energy resolution and better control over backgrounds. | Xun Chen Chang Bo Fu Javier Galan Karl Giboni Franco Giuliani Ling Hui Gu Ke Han Xiang Dong Ji Heng Lin Jiang Lai Liu Kai Xiang Ni Hiroki Kusano Xiang Xiang Ren Shao Bo Wang Yong Yang Dan Zhang Tao Zhang Li Zhao Xiang Ming Sun Shou Yang Hu Si Yu Jian Xing Long Li Xiao Mei Li Hao Liang Huan Qiao Zhang Ming Rui Zhao Jing Zhou Ya Jun Mao Hao Qiao Si Guang Wang Ying Yuan Meng Wang Amir N.Khan Neill Raper Jian Tang Wei Wang Jia Ning Dong Chang Qing Feng Cheng Li Jian Bei Liu Shu Bin Liu Xiao Lian Wang Dan Yang Zhu Juan F.Castel Susana Cebri'an Theopisti Dafni Javier G.Garza Igor G.Irastorza Francisco J.Iguaz Gloria Luz ′on Hector Mirallas Stephan Aune Eric Berthoumieux Yann Bedfer Denis Calvet Nicole d'Hose Alain Delbart Maria Diakaki Esther Ferrer-Ribas Andrea Ferrero Fabienne Kunne Damien Neyret Thomas Papaevangelou Franck Sabatie Maxence Vanderbroucke AnDi Tan Wick Haxton Yuan Mei Chinorat Kobdaj Yu-Peng Yan | 2017 | Science China(Physics,Mechanics & Astronomy)2017，60，6： | 2 |
| 8 | Some properties of the solution of a filtration problem in partially saturated porous media显示文摘 | An Lian Jun | 1984 | Acta Math Appl Sinica(English Series)1984，1，1： | 1 |
| 9 | Basic Fibroblast Growth Factor Protects C 17.2 Cells from Radiation‐Induced Injury through ERK 1/2显示文摘 | Ping Luan Hai‐Hong Zhou Bei Zhang An‐Ming Liu Lian‐Hong Yang Xue‐Ling Weng En‐Xiang Tao Jun Liu | 2012 | CNS Neurosci Ther2012，，： | 1 |
| 10 | Some properties of the solution of a filtration problem in partially saturated显示文摘 | An Lian Jun | 1984 | porous media Acta Math Appl Sinica(English Series)1984，，1： | 1 |
| 11 | Research Progress of Nuclear Astrophysics Physics:Progress of Jinping Underground Laboratory for Nuclear Astrophysics Experiment JUNA显示文摘1 Progress of this program This program is supported by the China Jinping Underground Laboratory(CJPL)and the direct measurement of stellar key reactions of(α,γ),(α,n),(p,γ)and(p,α)will be precisely carried out at the merit of current project by utilizing high stability and intensity accelerator,high efficiency detector and the shielding of extremely low background. | LIU Wei-ping LI Zhi-hong HE Jian-jun TANG Xiao-dong LIAN Gang GUO Bing AN Zhu CHEN Qing-hao CHEN Xiong-jun CHEN Yang-ping CHEN Zhi-jun CUI Bao-qun DU Xian-chao FU Chang-bo GAN Lin HAN Zhi-yu HE Guo-zhu A.Heger HOU Su-qing HUANG Han-xiong HUANG Ning JIA Bao-lu JIANG Li-yang S.Kubono LI Jian-min LI Kuo-ang LI Tao LI Xin-yue LI Yun-ju M.Lugaro LUO Xiao-bing MA Shao-bo MEI Dong-ming QIAN Yong-zhong QIN Jiu-chang REN Jie SU Jun SUN Liang-ting TAN Wan-peng I.Tanihata WANG Peng WANG You-bao WU Qi XU Shi-wei YAN Sheng-quan YANG Li-tao YU Xiang-qing YUE Qian ZENG Sheng ZHANG Huan-yu ZHANG Hui ZHANG Li-yong ZHANG Ning-tao ZHANG Qi-wei ZHANG Tao ZHANG Xiao-peng ZHANG Xue-zhen ZHANG Zi-min ZHAO Wei ZHAO Zhuo ZHOU Chao ZHOU Yong | 2015 | Annual Report of China Institute of Atomic Energy2015，，1： | 0 |
| 12 | Efficient Isomerization for Preparing thans-2,2-Dimethyl-3-(2,2-dichloroethenyl) cyclopropanecarbonyl Chloride显示文摘Trans-2,2-dimethyl-3-(2,2-dichloroethenyl)cyclopropanecarbonyl chloride, which is animportant intermediate for insecticides, is obtained by isomerization of the cis/trans-mixture with4- (N,N-dimethylamino) pyridine (DMAP) in the presence or absence of halogen compounds ingood yield. | Jun An MA Run Qiu HUANG Jun Ran CHENG Rui Lian SHAO Zai Guo LI(Institute and State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin300071) | 1999 | Chinese Chemical Letters1999，10，2： | 0 |
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