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| 1 | 临床致病性不动杆菌的分子生物学鉴定显示文摘目的对临床致病性不动杆菌进行种属鉴定,并比较3种方法(44℃生长试验、特异性引物PCR扩增和16SrRNA测序)对鲍曼不动杆菌鉴定的特异性、准确性及应用价值。方法收集临床致病不动杆菌56株,首先接种在胰蛋白胨大豆肉汤中44℃进行培养,观察是否可以生长;其次,提取56株不动杆菌基因组DNA,用两组不同的特异性引物进行PCR扩增鉴定;最后,用通用引物对56株不动杆菌的16SrRNA进行测序鉴定。结果 56株不动杆菌中有17株可在44℃生长。经特异性引物PCR扩增鉴定,发现8株鲍曼不动杆菌和3株13TU型不动杆菌均可在44℃中较早较快生长。金标准16SrRNA测序确定56株不动杆菌中含有9个种,其中鲍曼不动杆菌和13TU型不动杆菌的鉴定结果与特异性引物PCR扩增法相同。结论应用分子生物学方法鉴定鲍曼不动杆菌具有准确、高效且可重复性高的优点,尤其是特异性PCR扩增法,可作为较难诊断的鲍曼不动杆菌的首选鉴定方法。 | 韩蕾 张旭燕 韩少山 MCCARRON Andrew MOORE John E MILLAR Cherie B 徐纪茹 | 2012 | 西安交通大学学报(医学版)2012，33，5： | 4 |
| 2 | Whole brain radiation therapy with or without stereotactic radiosurgery boost for patients with one to three brain metastases: phase III results of the RTOG 9508 randomised trial显示文摘 | David W Andrews Charles B Scott Paul W Sperduto Adam E Flanders Laurie E Gaspar Michael C Schell Maria Werner-Wasik William Demas Janice Ryu Jean-Paul Bahary Luis Souhami Marvin Rotman Minesh P Mehta Walter J Curran | 2004 | The Lancet . 2004 (9422)2004，，9422： | 2 |
| 3 | Outcome Following Open Reduction and Internal Fixation of Open Pilon Fractures显示文摘 | SREEVATHSA B TRAVIS JK ANDREW E | 2010 | The Journal of Bone and Joint Surgery (American)2010，92，2： | 1 |
| 4 | Historical stor- age budgets of organic carbon, nutrient and contaminant elements in saltmarsh sediments: Biogeochemical context for managed realignment, Humber Estuary, UK显示文摘 | Andrews J E Samways G Shimmield G B | 2008 | Sci Total En- viron2008，405，： | 1 |
| 5 | Dual G1 and G2 phase inhibition by a novel selective CDC25 inhibitor 7-Chloro-6-(2-morpholin-4-ylethylamino) -quinoline-5,8-dione显示文摘 | LIXIA P ANDREW A A MARK E B | 2002 | J Biol Chem2002，277，46： | 1 |
| 6 | Effects of combine operating parameters on harvest loss and quality in rice显示文摘 | Andrews S B Siebenmorgen T J Vories E D | 1993 | Transaction of the ASAE1993，36，6： | 1 |
| 7 | AMPK and the neuroendocrine regulation of appetite and energy expenditure显示文摘 | Stark R Ashley S E Andrews Z B | 2013 | Molecular and Cellular Endocrinology2013，366，2： | 1 |
| 8 | ADVISOR-based model of a battery and an ultra-capacitor energy source for hybrid electric vehicles显示文摘 | Andrew C B Ali E | 2004 | IEEE Transactions on Vehicular Technology2004，53，1： | 1 |
| 9 | Measuring and simulating particulate organics in the atmosphere:problems and prospects显示文摘 | TURPIN B J SAXENA P ANDREWS E | 2000 | Atmospheric Environment2000，34，18： | 1 |
| 10 | Internal derangement of the wrist:indirect MR arthrography versus unenhanced MR imaging 显示文摘 | Andrew H Mark E William B | 2003 | Radiology2003，227，3： | 1 |
| 11 | Revenue management and e-commerce显示文摘 | ANDREW B E | 2003 | Management Science2003，49，： | 1 |
| 12 | The sorghum bicolor genome and the diversification of grasses显示文摘 | Andrew H P John E B Remy B | 2009 | Nature2009，457，： | 1 |
| 13 | Limitations of MR imaging in the diagnosis of peripheral tears of the triangular fibrocartilage of the wrist显示文摘 | Andrew H Mark E William B | 2002 | AJR2002，178，2： | 1 |
| 14 | Biomarker and n-alkane isotope profiles for Tertiary oils:relationship to source rock depositional setting显示文摘 | Andrew P M Roger E S Christopher J B | 1994 | Organic Geochemistry1994，22，35： | 1 |
| 15 | Tests on high- strength concrete-filled steel tubular columns 显示文摘 | Kilpatrick Andrew E Rangan B Vijaya | 1999 | ACI Structural Journal1999，96，2： | 1 |
| 16 | Aquanti- tative assayforcapacitation: evaluationof multiple spermpenetration through thezonapellucidaof salt2storedhamster eggs 显示文摘 | Boatman D E Andrews J C Bavister B D | 1988 | Gamete Res1988，19，1： | 1 |
| 17 | The functional anatomy and histology of the reproductive system of some pilid gastropod mollusks 显示文摘 | Andrews E B | 1964 | Proceedings of the Malacological Society1964，36，： | 1 |
| 18 | Pigment epithelium-derived factor regulates lipid metabolism via adipose triglyceride lipase显示文摘 | Borg M L Andrews Z B Duh E J | 2011 | Dia- betes2011，60，5： | 1 |
| 19 | Evaluation of selected crop water production functions for an irrigated maize crop显示文摘 | Igbadun H E Andrew K P R T Salim B A | 2007 | Agricultural Water Man- agement2007，94，3： | 1 |
| 20 | Measuring and simulating particulate organics in the atmosphere: Problems and prospects显示文摘 | Turpin B J Saxena P Andrews E | 2000 | Atmospheric Environment2000，34，18： | 1 |
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