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| 1 | Immunohistochemical expression of SP-NK-1R-EGFR pathway and VDR in colonic inflammation and neoplasia显示文摘AIM:To determine the expression of neurokinin-1receptor(NK-1R),phosphorylated epidermal growth factor receptor(p EGFR),cyclooxygenase-2(Cox-2),and vitamin D receptor(VDR)in normal,inflammatory bowel disease(IBD),and colorectal neoplasia tissues from Puerto Ricans.METHODS:Tissues from patients with IBD,colitisassociated colorectal cancer(CAC),sporadic dysplasia,and sporadic colorectal cancer(CRC),as well as normal controls,were identified at several centers in Puerto Rico.Archival formalin-fixed,paraffin-embedded tissues were de-identified and processed by immunohistochemistry for NK-1R,p EGFR,Cox-2,and VDR.Pictures of representative areas of each tissues diagnosis were taken and scored by three observers using a4-point scale that assessed intensity of staining.Tissues with CAC were further analyzed by photographing representative areas of IBD and the different grades of dysplasia,in addition to the areas of cancer,within each tissue.Differences in the average age between the five patient groups were assessed with one-way analysis of variance and Tukey-Kramer multiple comparisons test.The mean scores for normal tissues and tissues with IBD,dysplasia,CRC,and CAC were calculatedand statistically compared using one-way analysis of variance and Dunnett’s multiple comparisons test.Correlations between protein expression patterns were analyzed with the Pearson’s product-moment correlation coefficient.Data are presented as mean±SE.RESULTS:On average,patients with IBD were younger(34.60±5.81)than normal(63.20±6.13,P<0.01),sporadic dysplasia(68.80±4.42,P<0.01),sporadic cancer(74.80±4.91,P<0.001),and CAC(57.50±5.11,P<0.05)patients.NK-1R in cancer tissue(sporadic CRC,1.73±0.34;CAC,1.57±0.53)and sporadic dysplasia(2.00±0.45)were higher than in normal tissues(0.73±0.19).p EGFR was significantly increased in sporadic CRC(1.53±0.43)and CAC(2.25±0.47)when compared to normal tissue(0.07±0.25,P<0.05,P<0.001,respectively).Cox-2 was significantly increased in sporadic colorectal cancer(2.20±0.23 vs 0.80±0.37 for normal tissues,P<0.05).In comparison to normal(2.80±0.13)and CAC(2.50±0.33)tissues,VDR was significantly decreased in sporadic dysplasia(0.00±0.00,P<0.001 vs normal,P<0.001 vs CAC)and sporadic CRC(0.47±0.23,P<0.001 vs normal,P<0.001 vs CAC).VDR levels negatively correlated with NK-1R(r=-0.48)and p EGFR(r=-0.56)in normal,IBD,sporadic dysplasia and sporadic CRC tissue,but not in CAC.CONCLUSION:Immunohistochemical NK-1R and p EGFR positivity with VDR negativity can be used to identify areas of sporadic colorectal neoplasia.VDR immunoreactivity can distinguish CAC from sporadic cancer. | Raymond A Isidro Myrella L Cruz Angel A Isidro Axel Baez Axel Arroyo William A González-Marqués Carmen González-Keelan Esther A Torres Caroline B Appleyard | 2015 | World Journal of Gastroenterology2015，21，6： | 7 |
| 2 | Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease显示文摘AIM: To determine serum vitamin D levels and colonic vitamin D receptor(VDR) expression in inflammatory bowel disease(IBD) and non-IBD patients and correlate these with histopathology.METHODS: Puerto Rican IBD(n = 10) and non-IBD(n = 10) patients ≥ 21 years old scheduled for colonoscopy were recruited. Each patient completed a questionnaire and provided a serum sample and a colonic biopsy of normal-appearing mucosa. For IBD patients, an additional biopsy was collected from visually diseased mucosa. Serum vitamin D levels were measured by ultra-performance liquid chromatography and mass spectrometry. Hematoxylin and eosin stained tissue sections from colonic biopsies were classified histologically as normal or colitis(active/inactive), and scored for the degree of inflammation present(0-3, inactive/absent to severe). Tissue sections from colonic biopsies were also stained by immunohistochemistry for VDR, for which representative diagnostic areas were photographed and scored for staining intensity using a 4-point scale.RESULTS: The IBD cohort was significantly younger(40.40 ± 5.27, P < 0.05) than the non-IBD cohort(56.70 ± 1.64) with a higher prevalence of vitamin D deficiency(40% vs 20%, respectively) and insufficiency(70% vs 50%, respectively). Histologic inflammation was significantly higher in visually diseased mucosa from IBD patients(1.95 ± 0.25) than in normalappearing mucosa from control patients(0.25 ± 0.08, P < 0.01) and from IBD patients(0.65 ± 0.36, P < 0.05) and correlated inversely with VDR expression in visually diseased colonic tissue from IBD patients(r =-0.44, P < 0.05) and from IBD patients with Crohn's disease(r =-0.69, P < 0.05), but not in normal-appearing colonic tissue from control patients or IBD patients. Control and IBD patient serum vitamin D levels correlated positively with VDR expression in normal colon from control and IBD patients(r = 0.38, P < 0.05) and with patient age(r = 0.54, P < 0.01). CONCLUSION: Levels of serum vitamin D correlate positively with colonic VDR expression in visually normal mucosa whereas inflammation correlates negatively with colonic VDR expression in visually diseased mucosa in Puerto Rican patients. | Yamilka Abreu-Delgado Raymond A Isidro Esther A Torres Alexandra González Myrella L Cruz Angel A Isidro Carmen I González-Keelan Priscilla Medero Caroline B Appleyard | 2016 | World Journal of Gastroenterology2016，22，13： | 5 |
| 3 | Internet-based collaborative pseudo-dynamic testing of multi-span bridge structure显示文摘In this paper, the first Internet-based collaborative pseudo-dynamic testing of a multi-span bridge system in China's Mainland is presented in detail, which has been carried out by the collaboration of Hunan University (HNU), Harbin Institute of Technology (HIT) and Tsinghua University (TU) in China's Mainland, and the University of Southern California (USC) in the USA. The prototype system of Russion River Bridge at Southern California in America is illustrated. Different testing specimens, testing equipments and substructure boundary conditions at HNU and HIT laboratories are described. A network protocol platform for collaborative pseudo-dynamic testing, called NetSLab, and some function modules developed on the use of its standardized interface are introduced. Aiming at the MTS system widely used in structural testing, two approaches are presented to implement the communication interface between NetSLab and testing equipment, respectively, studied by HNU and HIT. The integrated networked testing system and collaborative testing procedures are presented. The testing results manifest that the networked collaborative testing system with powerful capability provides a testing platform with good coordination for complex models or actual structures, and achieves remote sharing of testing equipments. | Dapeng Wang a, Shizhu Tiana, Xinjiang Caia, Yunlei Fan b, Yan Xiaob,c a School of Civil Engineering, Harbin Institute of Technology, Harbin 150090, China b School of Civil Engineering, Hunan University, Changsha 410082, China c Department of Civil Engineering, University of Southern California, Los Angeles, CA 90089, USA | 2009 | Progress in Natural Science:Materials International2009，19，5： | 4 |
| 4 | Prognosis and follow-up of 135 patients with ischemic colitis over a five-year period显示文摘AIM:To study the prognosis(recurrence and mortality)of patients with ischemic colitis(IC).METHODS:This study was conducted in four Spanish hospitals,participants in the Ischemic Colitis in Spain study We analyzed prospectively 135 consecutive patients who met criteria for definitive or probable IC according to Brandt criteria,and follow up these patients during the next five years,retrospectively.Long-term results(recurrence and mortality)were evaluated retrospectively after a median interval of 62 mo(range54-75 mo).RESULTS:Estimated IC recurrence rates were 2.9%,5.1%,8.1%and 9.7%at years 1,2,3 and 5 years,respectively.Five-year survival was 69%(93 of 135)and 24%(10 of 42 patients)died for causes related to the IC.Among these 10 patients,8 died in their first episode at hospital(4 had gangrenous colitis and 4 fulminant colitis)and 2 due to recurrence.CONCLUSION:The five-year recurrence rate of IC was low.On the other hand,mortality during follow-up was high and was not associated with ischemic colitis. | Angel Cosme Miguel Montoro Santos Santolaria Ana B Sanchez-Puertolas Marta Ponce Margarita Durán Jose Luis Cabriada Nerea Borda Cristina Sarasqueta Luis Bujanda | 2013 | World Journal of Gastroenterology2013，19，44： | 2 |
| 5 | Rotavirus vac- cines: recent developments and future considerations 显示文摘 | ANGEL J FRANCO M A GREENBERG H B | 2007 | Nat Rev Microbiol2007，5，7： | 1 |
| 6 | Erlotinib inhibits progression to dysplasia in a colitis-associated colon cancer model显示文摘AIM:To investigate the role of epidermal growth factor receptor(EGFR) in colitis-associated dysplasia using the EGFR tyrosine kinase inhibitor erlotinib.METHODS:Sprague-Dawley rats received trinitrobenzene sulfonic acid(TNBS;30 mg in 50% ethanol,ic),followed 6 wk later by reactivation with TNBS(5 mg/kg,iv) for 3 d.To induce colitis-associated dysplasia,rats then received TNBS(iv) twice a week for 10 wk.One group received erlotinib(10 mg/kg,ip) for 1 wk before the start of the reactivation of the colitis and 2 wk after(21 d);the rest received the vehicle.After rats were euthanized,the colons were removed and analyzed for damage and expression of the EGFR downstream effectors Erk1/2 and c-Myc.RESULTS:Ninety percent of the vehicle-treated animals had dysplasia in any region of the colon.Erlotinib-treated animals had a significant decrease in the incidence of dysplasia compared to vehicle-treated animals in all regions of the colon(50.00% ± 11.47% vs 90.00% ± 10.00% in proximal,P < 0.05;15.00% ± 8.19% vs 50.00% ± 16.67% in mid,P < 0.05;and 20.00% ± 9.17% vs 70.00% ± 15.28% in distal,P < 0.01).Erlotinib-treated animals also had reduced cell proliferation,reduced active Erk1/2,and reduced c-Myc in colon epithelium compared with the vehicle-treated animals.In vitro,erlotinib treatment was shown to markedly decrease c-Myc and pErk1/2 levels in rat epithelial cells.Proliferation of rat epithelial cells was stimulated by epidermal growth factor and inhibited by erlotinib(P < 0.05).CONCLUSION:Erlotinib can decrease the development of colitis-associated dysplasia,suggesting a potential therapeutic use for erlotinib in patients with long-standing colitis. | Beatriz Pagán Angel A Isidro Myrella L Cruz Domenico Coppola Caroline B Appleyard | 2011 | World Journal of Gastroenterology2011，17，44： | 1 |
| 7 | An Evidence-based Project for Evaluating Strategies to Improve Knowledge Acquisition and Critical-thinking Performance in Nursing Students显示文摘 | Angel B F Duffey M Belyea M | 2000 | J Nurs Educ2000，39，5： | 1 |
| 8 | Theoretical com- parison of low GWP alternatives for different refrigeration configurations taking R404A as baseline显示文摘 | Adrian M B Joaquin N E Angel B | 2014 | International Journal of Refrigeration2014，44，1： | 1 |
| 9 | 显示文摘 | Sooklal K Cullum B S Angel S M | 1996 | J Phys Chem1996，100，11： | 1 |
| 10 | An integrated-circuit approach to extracellular microelectrodes 显示文摘 | WISE K D ANGELL J B STARR A | 1970 | IEEE Trans BiomedEng1970，17，3： | 1 |
| 11 | Toll-like receptor 2 participates in mediation of immune response in experimental pneumococcal meningitis显示文摘 | Koedel U Angele B Rupprecht T Wanger H Roggenkamp A Pfister HW | 2003 | J Immunol2003，170，1： | 1 |
| 12 | Relaxation in glassforming liquids and amorphous solids显示文摘 | Angell C A Ngai K L Mckenna G B | | 0，，： | 1 |
| 13 | Drop-in energy performance evaluation of R1234yf and R1234ze(E) in a vapor compression system as R134a replacements 显示文摘 | Adrian M B Joaquin N E Angel B | 2014 | Ap- plied Thermal Engineering2014，71，1： | 1 |
| 14 | Reconstruction of an extensive vulvectomy defect using the gluteus maximus fasciocutaneous V-Y advancement flap 显示文摘 | Arkoulakis NS Angel CL DuBeshter B | 2002 | Ann Plast Surg2002，49，1： | 1 |
| 15 | Innate immunity to pneumococcal infection of the central nervous system depends on toll-like receptor (TLR) 2 and TLR4 显示文摘 | Klein M Obermaier B Angele B | 2008 | J Infect Dis2008，198，7： | 1 |
| 16 | MRS of brain heman gioperreytomas: high myoinositol concentrations and discrimi nation from meningiom 显示文摘 | lgnas B Angel M Martiner P | 2001 | J Neuro surg2001，94，5： | 1 |
| 17 | Maternal serum androgens in pregnant women with polycystic ovarian syndrome: possible implications in prenatal androgenization 显示文摘 | Sir-Petermann T Maliqueo M Angel B | 2002 | Hum Reprod2002，17，10： | 1 |
| 18 | Differential regulation of blood-brain barrier permeability in brain trauma and pneumo- coccal meningitis-role of Src kinases显示文摘 | Paul R Angele B Popp B | 2007 | Exp Neurol2007，203，1： | 1 |
| 19 | Toll-like receptor 2 participates in mediation of immune response in experimental pneumococcal meningitis显示文摘 | Koedel U Angele B Rupprecht T | 2003 | Immunology2003，170，1： | 1 |
| 20 | Granulocyte inflammatory markers and airway infection during acute exacerbation of chronic obstructive pulmonary disease显示文摘 | AARON S D ANGEL J B LUNAU M | 2001 | Am J Respir Crit Care Med2001，163，： | 1 |
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