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| 1 | MicroRNAs as a potential prognostic factor in gastric cancer显示文摘AIM:To compare the microRNA (miR) profiles in the primary tumor of patients with recurrent and non-recurrent gastric cancer.METHODS:The study group included 45 patients who underwent curative gastrectomies from 1995 to 2005 without adjuvant or neoadjuvant therapy and for whom adequate tumor content was available.Total RNA was extracted from formalin-fixed paraffin-embedded tumor samples,preserving the small RNA fraction.Initial profiling using miR microarrays was performed to identify potential biomarkers of recurrence after resection.The expression of the differential miRs was later verified by quantitative real-time polymerase chain reaction (qRT-PCR).Findings were compared between patients who had a recurrence within 36 mo of surgery (bad-prognosis group,n=14,31%) and those who did not (good-prognosis group,n=31,69%).RESULTS:Three miRs,miR-451,miR-199a-3p and miR-195 were found to be differentially expressed in tumors from patients with good prognosis vs patients with bad prognosis (P<0.0002,0.0027 and 0.0046 respectively).High expression of each miR was associated with poorer prognosis for both recurrence and survival.Using miR-451,the positive predictive value for non-recurrence was 100% (13/13).The expression of the differential miRs was verified by qRT-PCR,showing high correlation to the microarray data and similar separation into prognosis groups.CONCLUSION:This study identified three miRs,miR-451,miR-199a-3p and miR-195 to be predictive of recurrence of gastric cancer.Of these,miR-451 had the strongest prognostic impact. | Baruch Brenner Moshe B Hoshen Ofer Purim Miriam Ben David Karin Ashkenazi Gideon Marshak Yulia Kundel Ronen Brenner Sara Morgenstern Marisa Halpern Nitzan Rosenfeld Ayelet Chajut Yaron Niv Michal Kushnir | 2011 | World Journal of Gastroenterology2011，17，35： | 31 |
| 2 | 斯德哥尔摩的拥挤收费政策——政策实施5年后的效果、接受度及经验启示显示文摘根据2006年拥挤收费政策试运行结束后的公民投票结果,2007年,斯德哥尔摩正式实施了该政策。讨论了其至2011年6月首个5年内的政策实施效果。结果表明,受到外部因素影响,由收费引起的交通减少量随时间推移稍有增加。2008年的清洁汽车免税措施可显著提高销售量。结合最新研究成果和瑞典的经验,讨论了公众接受度和政治接受度。得到的结论是:拥挤收费对交通系统的主客观影响,以及环境和政治态度,是公众支持拥挤收费的基础;同时,为了获得政治支持,必须要进行体制改革、解决权力问题。最后,简要讨论了拥挤收费的实施对瑞典交通规划进程的可能影响。 | Maria Brjesson Jonas Eliasson Muriel B.Hugsson Karin Brundell-Freij 刘莹 蔡静 | 2012 | 城市交通2012，10，2： | 10 |
| 3 | Participation of CD45,NKR-P1A and ANK61 antigen in rat hepatic NK cell(pit cell)-mediated target cell cytotoxicity显示文摘AIM Several triggering receptors have beendescribed to be involved in natural killer(NK)cell-mediated target cytotoxicity.In these studies,NKcells derived from blood or spleen were used.Pitcells are liver-specific NK cells that possess ahigher level of natural cytotoxicity and a differentmorphology when compared to blood NK cells.The aim of this study was to characterize the roleof the NK-triggering molecules NKR-P1A,ANK61antigen,and CD45 in pit cell-mediated killing oftarget cells.METHODS 51Cr-release and DNA fragmentationwere used to quantify target cell lysis andapoptosis,respectively.RESULTS Flow cytometric analysis showed thatpit cells expressed CD45,NKR-P1A,and ANK61antigen.Treatment of pit cells with monoclonalantibody(mAb)to CD45(ANK74)not onlyinhibited CC531s or YAC-1 target lysis but alsoapoptosis induced by pit cells.The mAbs to NKR-P1A(3.2.3)and ANK61 antigen(ANK61)had no effect on pit cell-mediated CC531s or YAC-1 targetcytolysis or apoptosis,while they did increase theFcγ receptor positive(FcγR+)P815 cytolysis andapoptosis.This enhanced cytotoxicity could beinhibited by 3,4-dichloroisocoumarin,an inhibitorof granzymes.CONCLUSION These results indicate that CD45participates in pit cell-mediated CC531s and YAC-1target cytolysis and apoptosis.NKR-P1A andANK61 antigen on pit cells function as activationstructures against FcγR+ P815 cells,which wasmediated by the perforin/granzyme pathway. | David Vermiilen Bülent Ahishali Vasilis Triantis Karin Vanderkerken Peter J. K.Kuppen Eddie Wisse | 2000 | World Journal of Gastroenterology2000，6，4： | 4 |
| 4 | Hepatic encephalopathy before and neurological complications after liver transplantation have no impact on the employment status 1 year after transplantation显示文摘AIM To investigate the impact of hepatic encephalopathybefore orthotopic liver transplantation(OLT) and neurological complications after OLT on employment after OLT.METHODS One hundred and fourteen patients with chronic liver disease aged 18-60 years underwent neurological examination to identify neurological complications, neuropsychological tests comprising the PSE-SyndromeTest yielding the psychometric hepatic encephalopathy score, the critical flicker frequency and the Repeatable Battery for the Assessment of Neuropsychological Status(RBANS), completed a questionnaire concerning their occupation and filled in the short form 36(SF-36) to assess health-related quality of life before OLT and 12 mo after OLT, if possible. Sixty-eight(59.6%) patients were recruited before OLT, while on the waiting list for OLT at Hannover Medical School [age: 48.7 ± 10.2 years, 45(66.2%) male], and 46(40.4%) patients were included directly after OLT. RESULTS Before OLT 43.0% of the patients were employed. The patients not employed before OLT were more often non-academics(employed: Academic/non-academic 16(34.0%)/31 vs not employed 10(17.6%)/52, P = 0.04), had more frequently a history of hepatic encephalopathy(HE)(yes/no; employed 15(30.6%)/34 vs not employed 32(49.2%)/33, P = 0.05) and achieved worse results in psychometric tests(RBANS sum score mean ± SD employed 472.1 ± 44.5 vs not employed 443.1 ± 56.7, P = 0.04) than those employed. Ten patients(18.2%), who were not employed before OLT, resumed work afterwards. The patients employed after OLT were younger [age median(range, min-max) employed 47(42, 18-60) vs not employed 50(31, 29-60), P = 0.01], achieved better results in the psychometric tests(RBANS sum score mean ± SD employed 490.7 ± 48.2 vs not employed 461.0 ± 54.5, P = 0.02) and had a higher health-related quality of life(SF 36 sum score mean ± SD employed 627.0 ± 138.1 vs not employed 433.7 ± 160.8; P < 0.001) compared to patients not employed after OLT. Employment before OLT(P < 0.001), age(P < 0.01) and SF-36 sum score 12 mo after OLT(P < 0.01) but not HE before OLT or neurological complications after OLT were independent predictors of the employment status after OLT.CONCLUSION HE before and neurological complications after OLT have no impact on the employment status 12 mo after OLT. Instead younger age and employment before OLT predict employment one year after OLT. | Henning Pflugrad Anita B Tryc Annemarie Goldbecker Christian P Strassburg Hannelore Barg-Hock Jürgen Klempnauer Karin Weissenborn | 2017 | World Journal of Hepatology2017，9，10： | 3 |
| 5 | Molecular phenotypes of human parvovirus B19 in patients with myocarditis显示文摘AIM:To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy(DCM).METHODS:Endomyocardial biopsies(EMBs) from 498 B19V-positive patients with myocarditis and DCMwere analyzed using molecular methods and functional experiments.EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers.Control tissues were obtained at autopsy from 34 victims of accidents,crime or suicide.Identification of mononuclear cell infiltrates in EMBs was performed using immunohistological staining.Anti-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay(ELISA).B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction(PCR).For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism(RFLP)-PCR was established.B19V-genotyping was verified by direct DNAsequencing and sequences were aligned using BLAST and BioEdit software.B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture experiments.Transfection experiments were conducted using human endothelial cells 1.Luciferase reporter assays were performed to determine B19Vreplication activity.Statistical analysis and graphical representation were calculated using SPSS and Prism5 software.RESULTS:The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy(iCMP) compared to uninflamed DCM(59.6% vs 35.3%)(P < 0.0001).The detection of B19V-mRNA replication intermediates proved that replication of B19V was present.RFLP-PCR assays showed that B19V-genotype 1(57.4%) and B19V-genotype 2(36.7%) were the most prevalent viral genotypes.B19V-genotype 2 was observed more frequently in EMBs with iCMP(65.0%) compared to DCM(35%)(P = 0.049).Although there was no significant difference in gender-specific B19V-loads,women were more frequently infected with B19V-genotype 2(44.6%) than men(36.0%)(P = 0.0448).Coinfection with B19V and other cardiotropic viruses was found in 19.2% of tissuesamples and was associated with higher B19V viral load compared to B19V-monoinfected tissue(P = 0.0012).The most frequent coinfecting virus was human herpes virus 6(HHV6,16.5%).B19V-coinfection with HHV6 showed higher B19V-loads compared to B19V-monoinfected EMBs(P = 0.0033),suggesting that HHV6 had transactivated B19V.In vitro experiments confirmed a 2.4-fold increased B19V P6-promoter activity by the HHV6 U94-transactivator.CONCLUSION:The finding of significantly increased B19V loads in patients with histologically proven cardiac inflammation suggests a crucial role of B19V-genotypes and reactivation of B19V-infection by HHV6-coinfection in B19V-associated iCMP.Our findings suggest that B19V-infection of the human heart can be a causative event for the development of an endothelial cell-mediated inflammatory disease and that this is related to both viral load and genotype. | C-Thomas Bock Anja Düchting Friederike Utta Eva Brunner Bui Tien Sy Karin Klingel Florian Lang Meinrad Gawaz Stephan B Felix Reinhard Kandolf | 2014 | World Journal of Cardiology2014，6，4： | 3 |
| 6 | Knowledge discovery from models of soil properties developed through data mining显示文摘 | Elisabeth N B Brent L H Karin V | 2006 | Ecological Modelling2006，191，34： | 1 |
| 7 | Comparison of different depletion strategies for improved resolution in proteomic analysis of human serum samples显示文摘 | Karin B Miliofis T Davidsson P | 2005 | Proteomics2005，5，： | 1 |
| 8 | Brain proteome aitemtions of Atlantic cod (Gadus morhua) exposed to PCB 153显示文摘 | Karin B P?I P Jarle K | 2011 | Aquatic Toxicology2011，105，34： | 1 |
| 9 | Obesity,inflammation,and liver cancer显示文摘 | Sun B Karin M | 2012 | J Hepatol2012，56，3： | 1 |
| 10 | Novel antiangiogenic effects of the bisphosphonate compound zoledronic acid 显示文摘 | KARINE B STEPHAN R | 2002 | J Pharmacol Exp Ther2002，302，： | 1 |
| 11 | Development of a finite element model of the upper cervieal spine and a parameter study of ligament characteristics显示文摘 | B Karin H Peter | 2004 | Spine2004，2，4： | 1 |
| 12 | The complexity of consumers' cognitive structures and its relevance to consumer behavior 显示文摘 | ZINKHAN G M KARIN B | 2004 | Journal of Business Research2004，57，： | 1 |
| 13 | Intrinsic resistance to apoptosis of colon epithelial cells is a potential determi- ning factor in the susceptibility of the a/j mouse strain to dimethyl- hydrazine - induced colon tumorigenesis显示文摘 | BOULARES A H KARINE O B MUSTAPHA O a al | 2007 | Mol Carcinogen2007，46，12： | 1 |
| 14 | Dehiscence and fenestration in pa- tients with class I and Class II Division 1 malocclusion assessed with cone-beam computed tomography显示文摘 | Karine E Karla FV Axel B | 2010 | American Journal of Orthodon- tics and Dentofacial Orthopedics2010，138，2： | 1 |
| 15 | Extraction of heavy metal from soil using biodegradable chelating agents显示文摘 | Susan T Karin B Roland M | 2004 | Environ Sci Technol2004，38，3： | 1 |
| 16 | Cytokine Response in Cerebrospinal Fluid after Brith Asphyxia显示文摘 | Karin S Mats B Katarina G | 1998 | Pediatr Res1998，43，6： | 1 |
| 17 | Minor improvement of venous blood specimen collection practices in primary health care after a large-scale educational intervention显示文摘 | Karin B?lenius Johan S?derberg Johan Hultdin Marie Lindkvist Christine Brulin Kjell Grankvist | 2013 | Clinical Chemistry and Laboratory Medicine2013，，2： | 1 |
| 18 | 母乳喂养史和子宫内膜异位症发生的风险:前瞻性队列研究显示文摘目的研究经产妇母乳喂养、纯母乳喂养、产后闭经时间与子宫内膜异位症发病率的关系。设计前瞻胜队列研究。 | Leslie V Farland A Heather Eliassen Rulla M Tamimi Donna Spiegelman Karin B Michels Stacey A Missmer 龚丽云 王淑珍 | 2018 | 英国医学杂志中文版2018，21，3： | 1 |
| 19 | Trace element speciation in combustion processes--Review and compilations of thermodynamic data显示文摘 | Karin L Anders N Rainer B | 2007 | Fuel Processing Technology2007，88，1112： | 1 |
| 20 | A spare parts model with cold-standby redundancy on system level 显示文摘 | Karin S S D Nicole P E Ana I B | 2011 | Computers : Operations Research2011，38，7： | 1 |
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