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| 1 | Chromosomal localization of the major ribosomal RNA genes in scallop Chlamys farreri显示文摘The chromosomes of Chlamys farreri were analyzed by means of silver staining and fluorescence in situ hybridization (FISH) with 18S—28S rDNA probe. Probe was made by PCR amplification of a DNA fragment containing internal transcribed spacers ITS1 between 18S and 5.8S ribosomal RNA gene, ITS2 between 5.8S and 28S ribosomal RNA gene and 5.8S rRNA gene, and labeled by PCR incorporation of bio-16-dUTP. FISH signals were located on the short arm of subtelocentric chromosome 10. After silver-staining, nucleolus organizer regions (NORs) could be observed on the telomere of the short arm of chromosome 10. However, one metaphase spread displayed an additional silver spot on the short arm of subtelocentric chromosome 12. | HUANG Xiaoting BAO Zhenmin BI Ke HU Jingjie ZHANG Can ZHANG Quanqi HU Xiaoli | 2006 | Acta Oceanologica Sinica2006，25，3： | 10 |
| 2 | Long-Term Cognitive Improvement After Benfotiamine Administration in Patients with Alzheimer's Disease显示文摘To date,we still lack disease-modifying therapies for Alzheimer's disease(AD).Here,we report that long-term administration of benfotiamine improved the cognitive ability of patients with AD.Five patients with mild to moderate AD received oral benfotiamine(300 mg daily) over 18 months.All patients were examined by positron emission tomography with Pittsburgh compound B(PiB-PET) and exhibited positive imaging with pamyloid deposition,and three received PiB-PET imaging at follow-up.The five patients exhibited cognitive improvement as assayed by the Mini-Mental Status Examination(MMSE) with an average increase of 3.2 points at month18 of benfotiamine administration.The three patients who received follow-up PiB-PET had a 36.7%increase in the average standardized uptake value ratio in the brain compared with that in the first scan.Importantly,the MMSE scores of these three had an average increase of 3 points during the same period.Benfotiamine significantly improved the cognitive abilities of mild to moderate AD patients independently of brain amyloid accumulation.Our study provides new insight to the development of diseasemodifying therapy. | Xiaoli Pan Zhichun Chen Guoqiang Fei Shumei Pan Weiqi Bao Shuhua Ren Yihui Guan Chunjiu Zhong | 2016 | Neuroscience Bulletin2016，32，6： | 9 |
| 3 | Existence of Microsatellites in Expressed Sequence Tags of Common Carp (Cyprinus carpio L.) Available in GenBank dbEST Database显示文摘表示顺序标注的普通鲤鱼(EST ) 为存在 ofmicrosatellites,或简单顺序重复(SSR ) 被分析。在 NCBI dbEST 数据库, 10612sequences 的一个总数在 2004 年 12 月 31 日前被登记。2-6 nucleotidemicrosatellites 的完全的搜索导致了 513 包含 SSR EST 的鉴定,为 4.8% 总数的财务。聚合分析显示包含 SSR EST 的 73 个序列掉进 27 个组并且留下 440 EST 是独立的。467 唯一的包含 SSR EST 的一个总数被识别。这些 EST-SSRs 包含了许多简单顺序类型,并且 di-nucleotiderepeats 和 tri-nucleotiderepeats 是最丰富的,为 42.1% 全部和 27.9% 的财务分别地。thedinucleotide 重复, CA/TG 是最丰富的,由 GA/TC 列在后面。BLASTx 搜索证明 SSR loci 的 that38.1% 能与基因或蛋白质被联系知道或包含 SSR EST 的未知 function.BLASTx 搜索也在 zebrafishsequences 上显示出点击的高频率(98/179 ) 。 | HU Jingjie WANG Xiaolong HU Xiaoli BAO Zhenmin | 2006 | Journal of Ocean University of China2006，5，1： | 8 |
| 4 | Habitat-induced reciprocal transformation in the root phenotype of Oriental ginseng is associated with alteration in DNA methylation显示文摘Oriental ginseng is an important medicinal plant that grows in 2 major forms or ecotypes,wild and domesticated.Each form differs conspicuously in root phenotype,but can be converted from one type to another by habitat.Here we show that the habi tat-induced transformation of ginseng root phenotype was accompanied by alteration in cytosine methylation at a large number of 5′-CCGG-3′sites detected by the methylation-sensitive polymorphism(MSAP)marker.The collective CG and CHG methylation levels of all 4 landraces of the domesticated form were significantly lower than those of the wild form.Interestingly,artificially transplanted ginseng plants recreated in both directions the methylation levels(at least in CHG)of their natural counterparts.The methylation differences between the 2 ginseng ecotypes were validated at 2 isolated MSAP loci bearing homology to a 5S rRNA gene or a copia retrotransposon.Our results implicate a link between epigenetic variation and habitat-induced phenotypic flexibility in Oriental ginseng. | NGEZAHAYO Frederic WANG XiaoLi YU XiaoMing JIANG LiLi CHU YunJie SHEN BaiHua YAN ZhongKai LIU Bao | 2011 | Chinese Science Bulletin2011，56，16： | 7 |
| 5 | Limited gene flow and partial isolation phylogeography of Himalayan snowcock Tetraogallus himalayensis based on part mitochondrial D-loop sequences显示文摘 | Xiaoli WANG Jiangyong QU Naifa LIU Xinkang BAO Sen SONG | 2011 | Current Zoology2011，57，6： | 5 |
| 6 | Ghrelin Reduces A-Type Potassium Currents in Dopaminergic Nigral Neurons via the PLC/PKCδPathway显示文摘Dear Editor,Ghrelin,an endogenous ligand of growth hormone secretagogue receptor 1a(GHS-R1a),is a 28-amino-acid peptide that regulates growth hormone secretion,metabolism,food intake,mood,cognition,memory,and neuroprotection[1-3]. | Bao Xue Chen Li Xiaoli Chang Hong Jiang Limin Shi Junxia Xie | 2020 | Neuroscience Bulletin2020，36，8： | 3 |
| 7 | Molecular identification of scallop planktonic larvae using species-specific microsatellites显示文摘The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However,the larvae identification by morphological characteristics is notoriously difficult,mainly due to the small size (usually being less than 150 μm) and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species,the Zhikong scallop Chlamys farreri,the noble scallop C. nobilis,the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers,which was specified by transferability analyses,assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1%~2% larvae in mixed plankton samples. Larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple,inexpensive and robust in identification analyses,and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years. | ZHAN Aibin HU Xiaoli BAO Lisui LU Wei PENG Wei WANG Mingling HU Jingjie | 2008 | Acta Oceanologica Sinica2008，27，5： | 3 |
| 8 | Development of 101 Novel EST-Derived Single Nucleotide Polymorphism Markers for Zhikong Scallop (Chlamys farreri)显示文摘Zhikong scallop(Chlamys farreri) is an important maricultured species in China.Many researches on this species,such as population genetics and QTL fine-mapping,need a large number of molecular markers.In this study,based on the expressed sequence tags(EST),a total of 300 putative single nucleotide polymorphisms(SNPs) were selected and validated using high resolution melting(HRM) technology with unlabeled probe.Of them,101(33.7%) were found to be polymorphic in 48 individuals from 4 populations.Further evaluation with 48 individuals from Qingdao population showed that all the polymorphic loci had two alleles with the minor allele frequency ranged from 0.046 to 0.500.The observed and expected heterozygosities ranged from 0.000 to 0.925 and from 0.089 to 0.505,respectively.Fifteen loci deviated significantly from Hardy-Weinberg equilibrium and significant linkage disequilibrate was detected in one pair of markers.BLASTx gave significant hits for 72 of the 101 polymorphic SNP-containing ESTs.Thirty four polymorphic SNP loci were predicted to be non-synonymous substitutions as they caused either the change of codons(33 SNPs) or pretermination of translation(1 SNP).The markers developed can be used for the population studies and genetic improvement on Zhikong scallop. | LI Jiqin BAO Zhenmin LI Ling WANG Xiaojian WANG Shi HU Xiaoli | 2013 | Journal of Ocean University of China2013，12，3： | 2 |
| 9 | Glutathione S-transferase genes in scallops and their diverse expression patterns after exposure to PST-producing dinoflagellates显示文摘The glutathione S-transferases(GSTs)are a superfamily of enzymes that function in cellular protection against toxic substances and oxidative stress.Bivalves could accumulate high concentration of paralytic shellfish toxins(PSTs)from harmful algae.To understand the possible involvement of GSTs in protecting bivalves during PST accumulation and metabolism,the GST genes were systemically analyzed in two cultured scallops,Azumapecten farreri and Mizuhopecten yessoensis,which were reported for PST deposition during harmful algae bloom.A total of 35 and 37 GSTs were identified in A.farreri(AfGSTs)and M.yessoensis(MyGSTs)genome,respectively,and the expansion of the sigma class from the cytosolic subfamily was observed.In both scallop species,sigma class GSTs showed higher expression than other members.The high GSTs expression was detected mainly during/after larvae stages and in the two most toxic organs,hepatopancreas and kidney.After ingesting PST-producing dinoflagellates,all the regulated AfGSTs in the hepatopancrcas were from the sigma class,but with opposite regulation pattern between Alexandrium catenella and A.minutum exposure.In scallop kidneys,where PSTs transformed into higher toxicity,more AfGSTs were regulated than in the hepatopancreas,and most of them were from the sigma class,with similar regulation pattern between A.catenella and A.minutum exposure.In M.yessoensis exposed to A.catenella,MyGST-σ2 was the only up-regulated MyGST in both hepatopancreas and kidney.Our results suggested the possible diverse function of scallop GSTs and the importance of sigma class in the defense against PSTs,which would contribute to the adaptive evolution of scallops in marine environments. | Jiarun Lou Jie Cheng Xiaogang Xun Xu Li Moli Li Xiangchao Zhang Tingting Li Zhenmin Bao Xiaoli Hu | 2020 | Marine Life Science & Technology2020，2，3： | 2 |
| 10 | An Efficient Procedure for Isolating Microsatellite DNAs from Sea Cucumber(Apostichopus japonicus)显示文摘The construction of enrichment library proves to be one of the efficient approaches for isolating microsatellites in this study. The genomic DNA of sea cucumber was digested with HaeIII and size-selected DNA fragments (250-700 bp) were ligated to an adaptor. Microsatellite-containing sequences were captured by using a combination of GA and CA probes, which were attached to a nylon membrane. The microsatellite enrichment library constructed in this study consisted of approximately 700 clones. Two hun-dred and thirty-two clones reacted positively after the library screening procedure. Of the 50 clones sequenced, all contained at least one microsatellite and one duplicate clone was found. Approximately 86% of the sequenced fragments permitted to design primers for sequence tagged microsatellite site (STMS). | HU Jingjie, ZHAN Aibin, LU Wei, HU Xiaoli, and BAO Zhenmin* Laboratory of Marine Genetics and Breeding (MGB), Division of Life Science and Biotechnology, Ocean University of China, Qingdao 266003, P. R. China | 2007 | Journal of Ocean University of China2007，6，1： | 2 |
| 11 | Development and Characterization of Microsatellite Markers for the Pacific Abalone (Haliotis discus) via EST Database Mining显示文摘The EST database of the Pacific abalone (Haliotis discus) was mined for developing microsatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more microsatellites. Based on the length and GC content of the flanking regions, cluster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify scorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Ho and He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification. | ZHAN Aibin BAO Zhenmin WANG Mingling CHANG Dan YUAN Jian WANG Xiaolong HU Xiaoli LIANG Chengzhu HU Jingjie | 2008 | Journal of Ocean University of China2008，7，2： | 2 |
| 12 | Cytogenetic analysis in two scallops (Bivalvia:Pectinidae) by PRINS and PI banding显示文摘Cytogenetic analysis was performed for the bay scallop(Argopecten irradians Lamarck 1819) and the Japanese scallop(Patinopecten yessoensis Jay 1857) by primed in situ labeling(PRINS) and propidium iodide(PI) banding techniques.The PRINS analysis revealed that major rRNA genes were clustered in two loci on the telomeric regions of the short arms on two acrocentric chromosome pairs in A.irradians and on two submetacentric pairs in P.yessoensis.The histone H3 gene sites differed in number and location between these two species.The C-band-like patterns revealed by PI staining varied considerably between these two species.A.irradians displayed terminal bands at long arms on all chromosomes,centromeric bands on some pairs and interstitial bands on five pairs.P.yessoensis exhibited only centromeric bands on all chromosomes.These results would contribute to the better understanding of karyotype evolution in A.irradians and P.yessoensis. | ZHANG Lingling BAO Zhenmin WANG Jue WANG Shi HUANG Xiaoting HU Xiaoli HU Jingjie | 2007 | Acta Oceanologica Sinica2007，26，5： | 2 |
| 13 | Association of myostatin Variants with Growth Traits of Zhikong Scallop(Chlamys farreri)显示文摘Scallop is a popular sea food and an important aquaculture shellfish.Identification of genes and genetic variants relating to scallop growth could benefit high-yielding scallop breeding.Myostatin(MSTN) is a conservative regulator of muscle growth,and has become one of the most important target genes for genetic improvement of the production of farmed animals.In this study,four single nucleotide polymorphisms(SNPs) were identified in the 5' flanking region of MSTN gene(Cf MSTN) in Zhikong scallop(Chlamys farreri).The association of these SNPs with scallop growth traits,including shell length,shell height,body weight and striated muscle weight was analyzed.The SNP g-1162G>T was found to associate with shell length,shell height,and striated muscle weight.The TT type scallops showed significantly higher trait values than those of GT type,and the GG type individuals exhibited median values.On the contrary,significantly more Cf MSTN transcripts were detected in the striated muscle of GT type scallops than in those of TT and GG type ones.Our results suggested that Cf MSTN might regulate the scallop muscle growth negatively,and SNP g-1162G>T can be used as a candidate marker for the selective breeding of high-yielding scallop. | FU Qiang GUO Huihui FENG Liying LI Xue ZHANG Lingling WANG Shi HU Xiaoli BAO Zhenmin | 2016 | Journal of Ocean University of China2016，15，1： | 2 |
| 14 | Antitumor and immunomodulatory activities of a polysaccharide from Artemisia argyi显示文摘 | Xiaoli Bao Chengzhong Wang Jinjin Liu Huihui Yuan Hongli Zhao Minbo Lan | 2013 | Carbohydrate Polymers2013，，： | 1 |
| 15 | Rice FLOURY ENDOSPERM22, encoding a pentatricopeptide repeat protein, is involved in both mitochondrial RNA splicing and editing and is crucial for endosperm development显示文摘Most of the reported P-type pentatricopeptide repeat(PPR) proteins play roles in organelle RNA stabilization and splicing. However, P-type PPRs involved in both RNA splicing and editing have rarely been reported, and their underlying mechanism remains largely unknown. Here, we report a rice floury endosperm22(flo22) mutant with delayed amyloplast development in endosperm cells. Map-based cloning and complementation tests demonstrated that FLO22 encodes a mitochondrion-localized P-type PPR protein.Mutation of FLO22 resulting in defective transsplicing of mitochondrial nad1 intron 1 and perhaps causing instability of mature transcripts affected assembly and activity of complex Ⅰ, and mitochondrial morphology and function. RNA-seq analysis showed that expression levels of many genes involved in starch and sucrose metabolism were significantly down-regulated in the flo22mutant compared with the wild type, whereas genes related to oxidative phosphorylation and the tricarboxylic acid cycle were significantly upregulated. In addition to involvement in splicing as a P-type PPR protein, we found that FLO22 interacted with DYW3, a DYW-type PPR protein, and they may function synergistically in mitochondrial RNA editing. The present work indicated that FLO22 plays an important role in endosperm development and plant growth by participating in nad1 maturation and multi-site editing of mitochondrial messager RNA. | Hang Yang Yunlong Wang Yunlu Tian Xuan Teng Zehui Lv Jie Lei Erchao Duan Hui Dong Xue Yang Yuanyan Zhang Yinglun Sun Xiaoli Chen Xiuhao Bao Rongbo Chen Chuanwei Gu Yipeng Zhang Xiaokang Jiang Wenyu Ma Pengcheng Zhang Yi Ji Yu Zhang Yihua Wang Jianmin Wan | 2023 | Journal of Integrative Plant Biology2023，65，3： | 1 |
| 16 | A rapid method for determination of Staphylococcus aureus based on milk coagulation by using a series piezoelectric quartz crystal sensor显示文摘 | Lili Bao Huwei Tan Qiong Duan Xiaoli Su Wanzhi Wei | 1998 | Analytica Chimica Acta1998，，1： | 1 |
| 17 | Enhanced oral bioavailability of silybin by a supersaturatable self-emulsifying drug delivery system (S-SEDDS)显示文摘 | Yinghui Wei Xiaoli Ye Xiaoguang Shang Xuan Peng Qiang Bao Minchen Liu Manman Guo Fanzhu Li | 2011 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2011，，： | 1 |
| 18 | Methods Comparison for Microsatellite Marker Development:Different Isolation Methods,Different Yield Efficiency显示文摘Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology,quantitative genetics and genomics. Therefore,it is extremely necessary to select several versatile,low-cost,efficient and time-and labor-saving methods to develop a large panel of microsatellite markers. In this study,we used Zhikong scallop(Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency,while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time-and cost-saving,it is difficult to obtain a large number of microsatellite markers,mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study,we recommend two methods,microsatellite-enriched library construction method and FIASCO-colony hybridization method,for large-scale microsatellite marker development. Both methods were derived from the microsatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes. | ZHAN Aibin BAO Zhenmin HU Xiaoli LU Wei HU Jingjie | 2009 | Journal of Ocean University of China2009，8，2： | 1 |
| 19 | Identification of phenolics in the fruit of emblica ( Phyllanthus emblica L.) and their antioxidant activities显示文摘 | Xiaoli Liu Chun Cui Mouming Zhao Jinshui Wang Wei Luo Bao Yang Yueming Jiang | 2008 | Food Chemistry2008，，4： | 1 |
| 20 | Residual strength of blast damaged reinforced concrete columns 显示文摘 | Bao Xiaoli Li Bing | 2010 | International Journal of Impact Engineering2010，37，4： | 1 |
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