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| 1 | Correction of β-thalassemia mutant by base editor in human embryos显示文摘 | Puping Liang Chenhui Ding Hongwei Sun Xiaowei Xie Yanwen Xu Xiya Zhang Ying Sun Yuanyan Xiong Wenbin Ma Yongxiang Liu Yali Wang Jianpei Fang Dan Liu Zhou Songyang Canquan Zhou Junjiu Huang | 2017 | Protein & Cell2017，8，11： | 34 |
| 2 | Effective gene editing by high-fidelity base editor 2 in mouse zygotes显示文摘通过相应再结合的指向的点 mutagenesis 广泛地在基因研究被使用了并且为在病人修理引起疾病的变化保持可观的诺言。然而,象 mosaicism 那样的问题和低 mutagenesis 效率继续提出挑战到如此的途径的临床的申请。最近,一个基础编辑器() 在 cytidine (C) 脱氨基酶上造的系统和 CRISPR/Cas9 技术在植物,酵母,和人的房间为指向的点 mutagenesis 作为一个其他的方法被开发。然而,基础编辑器高效地在 deamination 窗口中把 C 变换成 thymidine (T) 是否指向了在鼠标胚胎的基础编辑,仍然保持不清楚是可行的。在这份报告,我们产生了基础编辑的一个修改高保真版本 2 (HF2-BE2 ) ,并且调查了它在老鼠胚胎编辑功效的底。我们发现 HF2-BE2 能高效地把 C 变换成 T,与直到在老鼠胚胎的 100% biallelic 变化效率。不同于 BE3, HF2-BE2 能在目标和非目标海滨上把 C 变换成 T,扩展基础编辑器的编辑范围。令人惊讶地,我们发现 HF2-BE2 能也使脱去氨基对 gRNA 有约束力的区域近似的 C。一起拿,我们的工作表明由基础编辑,和下划线在鼠标产生点变化的可行性小心地优化编辑系统以便消除近似地点的 deamination 的底的需要。 | Puping Liang Hongwei Sun Ying Sun Xiya Zhang Xiaowei Xie Jinran Zhang zhen Zhang Yuxi Chen Chenhui Ding Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2017 | Protein & Cell2017，8，8： | 17 |
| 3 | Effective and precise adenine base editing n mouse zygotes显示文摘 | Puping Liang Hongwei Sun Xiya Zhang Xiaowei Xie Jinran Zhang Yaofu Bai Xueling Ouyang Shengyao Zhi Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2018 | Protein & Cell2018，9，9： | 4 |
| 4 | Profiling kidney microRNAs from juvenile grass carp(Ctenopharyngodon idella) after 56 days of oral exposure to decabromodiphenyl ethane显示文摘Grass carp(Ctenopharyngodon idella) is one of the most important species in China.Decabromodiphenyl ethane(DBDPE) is a brominated flame retardant that has been used widely in industry, and has been observed to accumulate in the tissues of fish from South China. Evidence has shown that DBDPE is toxic to aquatic animals, but the molecular response has been unclear. MicroRNAs(miRNAs) are small noncoding and negative regulatory RNAs that are 20–24 nucleotides in length, which are involved in a wide range of biological processes. We took advantage of deep-sequencing techniques to accurately and comprehensively profile the kidney mi RNA expression of grass carp after 8 weeks of oral exposure to DBDPE. After mapping sequencing data to the genome and Expressed Sequence Tags(ESTs) of grass carp, we identified 493 miRNAs in the sequenced grass carp samples, which included 51 new miRNAs. The results indicated that 5 miRNAs were significantly down-regulated and 36 miRNAs were significantly up-regulated(FDR < 0.001,1.5-fold change) after DBDPE exposure. Real-time quantitative PCR(RT-q PCR) was performed on 4 miRNAs from the two samples, and the sequencing and RT-q PCR data were consistent. This study provides the first comprehensive identification of grass carp miRNAs, and the first expression analysis of grass carp miRNAs following DBDPE exposure.The results indicated that miRNAs have potential for use as biomarkers. | Lian Gan Yuanyan Xiong Fang Dong Yunjiang Yu Lijuan Zhang Shunmei E. Liliu Zhou Xiaoxia Li Guocheng Hu | 2016 | Journal of Environmental Sciences2016，28，6： | 4 |
| 5 | A new trimeric furanocoumarin from Heracleum rapula显示文摘新 trimeric 线性类型的 furanocoumarin, rapultririn A (1 ) ,从 Heracleum rapula 的地下的部分被孤立。这新混合物的结构被 NMR 阐明, MS 分光镜的方法。 | Cun Zhang YuanYan Liu Yong Qing Xiao Li Li | 2009 | Chinese Chemical Letters2009，20，9： | 2 |
| 6 | Multiseale fa- cial structure representation for face recognition under varying illumination 显示文摘 | Zhang Taiping Tang Yuanyan Fang Bin | 2009 | Pattern Reeagnition2009，42，2： | 1 |
| 7 | Topology Preserving Non-negative Matrix Factorization for Face Recognition显示文摘 | Zhang Taiping Fang Bin Tang Yuanyan | 2008 | IEEE Trans on Image Processing2008，17，4： | 1 |
| 8 | Rice FLOURY ENDOSPERM22, encoding a pentatricopeptide repeat protein, is involved in both mitochondrial RNA splicing and editing and is crucial for endosperm development显示文摘Most of the reported P-type pentatricopeptide repeat(PPR) proteins play roles in organelle RNA stabilization and splicing. However, P-type PPRs involved in both RNA splicing and editing have rarely been reported, and their underlying mechanism remains largely unknown. Here, we report a rice floury endosperm22(flo22) mutant with delayed amyloplast development in endosperm cells. Map-based cloning and complementation tests demonstrated that FLO22 encodes a mitochondrion-localized P-type PPR protein.Mutation of FLO22 resulting in defective transsplicing of mitochondrial nad1 intron 1 and perhaps causing instability of mature transcripts affected assembly and activity of complex Ⅰ, and mitochondrial morphology and function. RNA-seq analysis showed that expression levels of many genes involved in starch and sucrose metabolism were significantly down-regulated in the flo22mutant compared with the wild type, whereas genes related to oxidative phosphorylation and the tricarboxylic acid cycle were significantly upregulated. In addition to involvement in splicing as a P-type PPR protein, we found that FLO22 interacted with DYW3, a DYW-type PPR protein, and they may function synergistically in mitochondrial RNA editing. The present work indicated that FLO22 plays an important role in endosperm development and plant growth by participating in nad1 maturation and multi-site editing of mitochondrial messager RNA. | Hang Yang Yunlong Wang Yunlu Tian Xuan Teng Zehui Lv Jie Lei Erchao Duan Hui Dong Xue Yang Yuanyan Zhang Yinglun Sun Xiaoli Chen Xiuhao Bao Rongbo Chen Chuanwei Gu Yipeng Zhang Xiaokang Jiang Wenyu Ma Pengcheng Zhang Yi Ji Yu Zhang Yihua Wang Jianmin Wan | 2023 | Journal of Integrative Plant Biology2023，65，3： | 1 |
| 9 | An improved LDA ap- proach显示文摘 | Jing Xiaoyuan Zhang D Tang Yuanyan | 2004 | IEEE Trans on Systems Man and Cybernetics Part B : Cybernetics2004，34，5： | 1 |
| 10 | A Fourier-LDA approach for image recognition显示文摘 | Jing Xiaoyuan Tang Yuanyan Zhang David | 2005 | Pattern Recognition2005，38，： | 1 |
| 11 | Face recognition under varying illumination using gradient faces显示文摘 | Zhang Taiping Tang Yuanyan Fang Bin | 2009 | IEEE Transactions on Image Processing2009，18，11： | 1 |
| 12 | Effects of Roudoukou-8 San Extract on Hydrogen Peroxide Induced Cardiomyocyte Injury显示文摘[Objectives] To study the effects of Roudoukou-8 San extract on hydrogen peroxide( H_2O_2) induced cardiomyocyte injury of rats and explore its action mechanism. [Methods] The cardiomyocytes of neonatal rats were isolated and cultured,and the H_2O_2 induced cardiomyocyte injury model was established. Methyl thiazolyl tetrazolium( MTT) assay was used to detect the protective effects of Roudoukou-8 San extract on H_2O_2 induced cardiomyocyte. The effects of Roudoukou-8 San on cardiomyocyte morphology were observed under inverted microscope. The contents of lactate dehydrogenase( LDH),creatine kinase( CK) and aspartate aminotransferase( AST) in cell culture medium were determined by automatic biochemical instrument; the levels of malondialdehyde( MDA),superoxide dismutase( SOD) and nitric oxide( NO) in the cells were detected by kit method. The apoptotic morphology of cardiomyocytes was observed by Hoechst fluorescence staining.Cell apoptosis were measured by Annexin V and PI double staining and flow cytometer. [Results]100 μmol/L of H_2O_2 acting 2 h could cause about 50% of cardiomyocyte injury. H_2O_2 model group showed increased cell gap,decreased cell count,cell cytoplasmic vacuoles and other obvious damages. Compared with H_2O_2 model group,in Roudoukou-8 San extract dose groups,cardiomyocyte morphology showed different degrees of improvement,Roudoukou-8 San extract can significantly reduce the content of LDH,CK and AST in H_2O_2 injured myocardial cell culture solution,significantly reduce content of MDA and NO in H_2O_2 injured myocardial cells,increase the SOD activity,and significantly inhibit the apoptosis of H_2O_2 injured myocardial cells. [Conclusions]Through improving the cell survival status,increasing the cell viability,reducing oxidative stress injury,inhibiting inflammatory responses,and inhibiting apoptosis,Roudoukou-8 San extract can improve the state of H_2O_2 induced cardiomyocyte injury,so as to protect H_2O_2 injured myocardial cells. | Yunfeng XIAO Yuanyan ZHANG Wenyan LI Yuhua WANG | 2018 | Medicinal Plant2018，9，5： | 1 |
| 13 | Study on Mechanisms of Cd^(2+) Sorption on Goethite byMicrocalorimetry显示文摘A study on energy changes and mechanisms of Cd sorbed on goethite was performed using the technique ofmicrocalorimetry. The data of the amount of Cd sorption (Aq) and concentration of Cd in equilibrium solution(Ce), and the data of Aq and the heat effect (AH) caused by Cd^2+ sorption on goethite all fitted Langmuirisotherm. The amount of heat released from Cd sorption on goethite increased with the amount of Ce or Aq.The reaction process of Cd sorption on goethite may be divided into five stages and three plateaus, dependingon the variation of enthalpy change (.Hm) of Cd sorption with Aq, which implied three mechanisms ofinteraCtion between Cd and goethite. The experimental results showed that the microcalorimetry may beuseful for determination of microcalorie variation in soil. | ZHANG GUIYIN WANG TIANZHI DONG YUANYAN LI XUEYUANI(1Huazhong Agricultural University, Wuhan 43OO7O (China))(2Agricultural University of Hebei, Baoding 071001 (China))(3 Wuhan University, Wuhan 450072 (China)) | 1999 | Pedosphere1999，9，4： | 1 |
| 14 | Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation显示文摘Understanding the regulatory networks for germ cell fate specification is necessary to developing strategies for improving the efficiency of germ cell production in vitro.In this study,we developed a coupled screening strategy that took advantage of an arrayed bi-molecular fluorescence complementation(BiFC)platform for protein-protein interaction screens and epiblast-like cell(EpiLC)-induction assays using reporter mouse embryonic stem cells(mESCs).Investigation of candidate interaction partners of core human pluripotent factors OCT4,NANOG,KLF4 and SOX2 in EpiLC differentiation assays identified novel primordial germ cell(PGC)-inducing factors including BEN-domain(BEND/Bend)family members.Through RNA-seq,ChIP-seq,and ATAC-seq analyses,we showed that Bend5 worked together with Bend4 and helped mark chromatin boundaries to promote EpiLC induction in vitro.Our findings suggest that BEND/Bend proteins represent a new family of transcriptional modulators and chromatin boundary factors that participate in gene expression regulation during early germline development. | Guang Shi Yaofu Bai Xiya Zhang Junfeng Su Junjie Pang Quanyuan He Pengguihang Zeng Junjun Ding Yuanyan Xiong Jingran Zhang Jingwen Wang Dan Liu Wenbin Ma Junjiu Huang Zhou Songyang | 2022 | Protein & Cell2022，13，10： | 1 |
| 15 | Topology preserving non-negative matrix factorization for face recognition显示文摘 | Zhang Taiping Fang Bin Tang Yuanyan | 2008 | IEEE Transactions on Image Processing2008，17，4： | 1 |
| 16 | A Fourier-LDA approach for image recognition 显示文摘 | Jing XiaoYuan Tang YuanYan Zhang David | 2005 | Pattern Recognition2005，38，3： | 1 |
| 17 | BRITTLE PLANT1 is required for normal cell wall composition and mechanical strength in rice显示文摘A series of nucleotide sugar interconversion enzymes(NSEs) generate the activated sugar donors required for biosynthesis of cell wall matrix polysaccharides and glycoproteins. UDPglucose 4-epimerases(UGEs) are NSEs that function in the interconversion of UDP-glucose(UDP-Glc) and UDP-galactose(UDP-Gal). The roles of UDP-glucose 4-epimerases in monocots remain unclear due to redundancy in the pathways. Here, we report a brittle plant(bp1) rice mutant that exhibits brittle leaves and culms at all growth stages. The mutant culms had reduced levels of rhamnogalacturonan I, homogalacturonan, and arabinogalactan proteins.Moreover, the mutant had altered contents of uronic acids, neutral noncellulosic monosaccharides, and cellulose. Map-based cloning demonstrated that OsBP1 encodes a UDPglucose 4-epimerase(OsUGE2), a cytosolic protein. We also show that BP1 can form homoand hetero-protein complexes with other UGE family members and with UDP-galactose transporters 2(OsUGT2) and 3(OsUGT3), which may facilitate the channeling of Gal to polysaccharides and proteoglycans. Our results demonstrate that BP1 participates in regulating the sugar composition and structure of rice cell walls. | Yuanyan Zhang Yihua Wang Chunming Wang Carsten Rautengarten Erchao Duan Jianping Zhu Xiaopin Zhu Jie Lei Chao Peng Yunlong Wang Xuan Teng Yunlu Tian Xi Liu Joshua LHeazlewood Aimin Wu Jianmin Wan | 2021 | Journal of Integrative Plant Biology2021，63，5： | 1 |
| 18 | Face recognition under varying illumination using gradientfaces 显示文摘 | ZHANG Taiping TANG Yuanyan FANG Bin SHANG Zhaowei LIu Xiaoyu | 2009 | IEEE Transac- tions on Image Processing2009，18，11： | 1 |
| 19 | Face recognition under varying illumination using gradientfaces显示文摘 | Zhang Taiping Tang Yuanyan Fang Bin | 2009 | IEEE Transactions on Image Processing2009，18，11： | 1 |
| 20 | Multiscale facial structure representation for face recognition under varying illumination 显示文摘 | ZHANG Taiping FANG Bin YUAN Yuan TANG Yuanyan SHANG Zhaowei L~ Donghui LANG Fang- nian | 2009 | Pat- tern Recognition2009，42，2： | 1 |
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