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| 1 | Modulation of ovine SBD-1 expression by 17heta-estradiol in ovine oviduct epithelial cells显示文摘 | Wen Shiyong CAO Guifang Bao Tuya | 2012 | BMC Veterinary Research2012，8，1： | 1 |
| 2 | Modulation of o- vine SBD-1 expression by 17 beta-estradiol in ovine oviduct epithelial cells 显示文摘 | Wen Shiyong Cao Guitang Bao Tuya | 2012 | BMC Veterinary Research2012，8，： | 1 |
| 3 | Molecular-level effects of acupuncture on depression: a genome-wide transcriptome analysis of pituitary gland in rats exposed to chronic restraint stress显示文摘OBJECTIVE: To investigate the antidepressant mechanism of acupuncture at the molecular level in the pituitary gland of rats exposed to chronic restraint stress(CRS).METHODS: Forty-eight rats were randomly divided into a control group(C), model group(M), fluoxetine group(F), and acupuncture group(A). CRS was induced in the model group by restraining rats for 28 days. Groups F and A were given fluoxetine administration and acupuncture intervention, respectively, 1 h before the CRS procedures were implemented. On the 29 th day, rat pituitary glands were harvested for RNA extraction and RNA-sequencing analysis.RESULTS: Differentially expressed gene analysis identified 148, 87, and 179 response genes differen-tially expressed in group C vs group M, group F vs group M, and group A vs group M, respectively. Using Gene Ontology term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, we identified the up-regulation of gene sets involved in extracellular space,receptor binding, and monoamine neurotransmitter metabolism, and the down-regulation of gene sets involved in immune response and inflammatory response as the prevailing transcriptomic signatures in the pituitary gland of rats treated with CRS,fluoxetine, or acupuncture.CONCLUSION: Our findings suggest that acupuncture has a multitarget antidepressant effect at the molecular and behavioral levels. | Wang Yu Jiang Huili Meng Hong Li Jing Yang Xinjing Zhao Bingcong Yu Qiuyun Liang Xingchen Bao Tuya | 2017 | Journal of Traditional Chinese Medicine2017，37，4： | 0 |
| 4 | Mechanisms of Up-regulation of 17β-Estrodiol on β-Defensin-2 ( SBD-2) Expression in Epithelial Cells of Ovine Oviduct显示文摘[Objective]To investigate the mechanisms involved in the Up-regulatory effects of 17β-estrodiol on β-defensin-2( SBD-2) in epithelial cells of ovine oviduct. [Methods]Epithelial cells of ovine oviduct were isolated and cultured; and then the cultured cells at secondary generation were divided into 17β-estradiol( E2,10- 8mol / L) group,estrogen nuclear receptor antagonist ICI182780( 10- 7mol / L) group,PKA antagonist KT-5720( 1 μmol / L) group,PKC antagonist H-7( 50 μmol / L) group,nuclear factor kappa B antagonist PDTC( 50 μmol / L) group and the blank control group( Control). Firstly,different antagonists were added into corresponding antagonist groups in order to interfere the epithelial cells of ovine oviduct for 1 h. Then,17β-estradiol( 10- 8mol / L) was added into each antagonist group and E2 group for cultivation for 6 h. Finally,real-time fluorescent quantitative RT-PCR was used to detect the changes of SBD-2 mRNA expression.[Results]10- 8mol / L 17β-estrodiol had significantly Up-regulatory effects on the expression of SBD-2 mRNA( P < 0. 05). Estrogen nuclear receptor antagonist ICI182780,NF-κΒ antagonist PDTC and PKC antagonist H-7 could all block the Up-regulatory effects on SBD-2. But PKA antagonist KT-5720 showed no significant effects on the Up-regulation of SBD-2 mRNA expression induced by 17β-estrodiol. [Conclusions] SBD-2 mRNA expression induced by 17β-estrodiol in epithelial cells of ovine oviduct was mediated by estrogen nuclear receptor ICI182780,NF-κB and PKC pathways. However,PKA pathway might not participate in the Up-regulation of SBD-2 mRNA expression. | Bao Tuya Cao Guifang Tu Yong Du Chenguang | 2015 | Animal Husbandry and Feed Science2015，7，2： | 0 |
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