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| 1 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 2 | Electroacupuncture alters pain-related behaviors and expression of spinal prostaglandin E_2 in a rat model of neuropathic pain显示文摘OBJECTIVE: To investigate the role of spinal prostaglandin E_2(PGE_2) in electroacupuncture(EA) analgesia and assess the theoretical basis for selection of acupoints in the treatment of neuropathic pain.METHODS: A rat model of neuropathic pain was established. Rats were randomly divided into normal,model, sham, EA 1, EA 2, and EA 3 groups. In EA 1group, the rats were needled at bilateral L5 Jiaji(EX-B2), Dachangshu(BL 25), Weizhong(BL 40) and Kunlun(BL 60). In EA 2 group, the rats were needled at bilateral Weizhong(BL 40) and Kunlun(BL60). In EA 3 group, the rats were needled at bilateral L5 Jiaji(EX-B2) and Dachangshu(BL 25). EA stimulation was administered once daily over 7 days. Motor function and thermal withdrawal latencies were evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. After 7 days of intervention,enzyme-linked immunosorbnent assay(ELISA) was used to quantify the expression of the spinal PGE_2.RESULTS: Rats in the model group exhibited evident hyperalgesia in responses to thermal withdrawal latencies compared with those in the control group(P < 0.01), and EA reversed thermal withdrawal latencies(P < 0.01). The expression level of the spinal PGE_2 was significantly higher in the model group than that in the control group and was reversed by EA(P < 0.01; P < 0.05).CONCLUSION: The effect of EA on neuropathic pain might alleviate the hyperalgesia state by an inhibition of local prostaglandin E_2 secretion. | Jiang Huili Yu Xue Ren Xiujun Tu Ya | 2016 | Journal of Traditional Chinese Medicine2016，36，1： | 19 |
| 3 | Clinical characteristics and effectiveness of antiviral agents in hospitalized children with infectious mononucleosis in China:A multicenter retrospective study显示文摘Importance:The clinical characteristics of infectious mononucleosis(IM)in Chinese children have not been evaluated in multicenter studies,and the effectiveness of antiviral treatment are controversial.Objective:To investigate the clinical characteristics of Chinese children with IM and current status of antiviral therapy for affected patients.Methods:Hospitalized patients with IM were enrolled between 2018 and 2020 in five children’s hospitals in China.The clinical characteristics were compared among four age groups:<3 years,3-<6 years,6-<10 years,and≥10 years.The clinical characteristics of IM and effectiveness of antiviral therapy were compared among patients receiving acyclovir(ACV),ganciclovir(GCV),and no antiviral therapy(i.e.,non-antiviral group).Results:In total,499 patients were analyzed;most patients were 3-<6 years of age.The most common symptoms and signs included fever(100%),lymphadenopathy(98.6%),pharyngitis(86.4%),eyelid edema(76.8%),and snoring(72.9%).There were significant differences in rash,hepatomegaly,and liver dysfunction among the four age groups.Patients aged<3 years had a lower incidence of liver dysfunction and a higher incidence of rash.Among the 499 patients,50.1%were treated with GCV,26.3%were treated with ACV,and 23.6%received no antiviral therapy.Compared with the non-antiviral group,patients in the ACV and GCV groups had longer durations of fever(P<0.001).There were no significant differences in the incidences of complications among the three treatment groups.Interpretation:The incidence of IM in Chinese children peaked at 3-<6 years of age.Clinical features of IM varied according to age.Patients receiving antiviral therapy exhibited more serious clinical manifestations than did patients without antiviral therapy.The effectiveness of antiviral therapy for IM requires further analysis. | Huili Hu Huiling Deng Jing Bi Yi Xu Shuangjie Li Yue Xie Xinrong Sun Dongmeng Wang Xufang Li Wenxian Ouyang Bing Hu Yufeng Zhang He Tang Chunxiao Fang Hui Zhang Lingyun Guo Chen Wang Tianyi Wang Fengxia Yang Tao Jiang Zhengde Xie Gang Liu | 2021 | Pediatric Investigation2021，5，3： | 12 |
| 4 | Economic Value Evaluation of Wetland Service in Yeyahu Wetland Nature Reserve,Beijing显示文摘Based on multi-source data,this paper evaluated the economic value of ecological services in the Yeyahu Wetland Nature Reserve,Beijing,China.The ecological services of wetland included gas regulation,water quality im-provement,biodiversity maintenance,erosion control,water supply,recreational opportunity,raw material supply and existence value.Multiple conventional evaluation methods were used to calculate the value of eight wetland services.The results showed that significant values came from biodiversity maintenance and recreational opportunity.The main reasons were as follows.Firstly,Yeyahu Wetland Nature Reserve was the habitat for migrant birds,and government had payed more efforts to protect precious birds.Secondly,the population is large in Beijing.People enjoyed going outside and enjoyed the natural and artificial wetland scenes.At the same time,most people were prepared to pay for wetland conservation.The decline of vegetation cover made the economic value of erosion control the lowest.While the shrink of water resource and the deteriorative water quality caused the economic value of water supply lower.The evaluation results could help decision-makers understand the present status of the Yeyahu Wetland Nature Reserve and provide a scientific basis for strategic decision. | ZHU Lin CHEN Yun GONG Huili JIANG Weiguo ZHAO Wenji XIAO Yanfang | 2011 | Chinese Geographical Science2011，21，6： | 7 |
| 5 | CUL4B facilitates HBV replication by promoting HBx stabilization显示文摘Objective:Hepatitis B virus(HBV)infection is a major public health problem worldwide.However,the regulatory mechanisms underlying HBV replication remain unclear.Cullin 4 B-RING ubiquitin E3 ligase(CRL4 B)is involved in regulating diverse physiological and pathophysiological processes.In our study,we aimed to explain the role of CUL4 B in HBV infection.Methods:Cul4 b transgenic mice or conditional knockout mice,as well as liver cell lines with CUL4 B overexpression or knockdown,were used to assess the role of CUL4 B in HBV replication.Immunoprecipitation assays and immunofluorescence staining were performed to study the interaction between CUL4 B and HBx.Cycloheximide chase assays and in vivo ubiquitination assays were performed to evaluate the half-life and the ubiquitination status of HBx.Results:The hydrodynamics-based hepatitis B model in Cul4 b transgenic or conditional knockout mice indicated that CUL4 B promoted HBV replication(P<0.05).Moreover,the overexpression or knockdown system in human liver cell lines validated that CUL4 B increased HBV replication in an HBx-dependent manner.Importantly,immunoprecipitation assays and immunofluorescence staining showed an interaction between CUL4 B and HBx.Furthermore,CUL4 B upregulated HBx protein levels by inhibiting HBx ubiquitination and proteasomal degradation(P<0.05).Finally,a positive correlation between CUL4 B expression and HBV pg RNA level was observed in liver tissues from HBV-positive patients and HBV transgenic mice.Conclusions:CUL4 B enhances HBV replication by interacting with HBx and disrupting its ubiquitin-dependent proteasomal degradation.CUL4 B may therefore be a potential target for anti-HBV therapy. | Haixia Shan Bo Wang Xiaodong Zhang Hui Song Xi Li Yongxin Zou Baichun Jiang Huili Hu Hao Dou Changshun Shao Lifen Gao Chunhong Ma Xiaoyun Yang Xiaohong Liang Yaoqin Gong | 2022 | Cancer Biology & Medicine2022，19，1： | 2 |
| 6 | Effect of electroacupuncture on E-prostanoid 2 expression in the spinal cord in rats with neuropathic pain显示文摘OBJECTIVE: To observe the effect of electroacupuncture(EA) treatment on the expression of E-prostanoid 2(EP2) in the spinal cord using a rat model of neuropathic pain, and to examine the effect of different acupuncture prescriptions.METHODS: Rats were randomly divided into six groups: a normal group, model group, sham group,EA 1 group(distal-proximal acupoints), EA 2 group(proximal acupoints), and EA 3 group(distal acupoints). Rats in the EA groups were treated for20 min, once daily for 7 days. The mechanical withdrawal threshold was evaluated at 1 day preoperatively and at 3, 5, and 7 days postoperatively. At 7days postoperatively, hematoxylin-eosin(HE) staining was used to investigate spinal morphological changes. Spinal EP2 messenger ribonucleic acid(m RNA) was detected using reverse-transcription polymerase chain reaction.RESULTS: After the EA treatment, the mechanical withdrawal threshold was up-regulated compared with the model group(P < 0.05). The expression of EP2 m RNA was up-regulated in the spinal cord on day 7 after surgery(P < 0.05). EA stimulation tended to down-regulate EP2 m RNA expression compared with the model group(P > 0.05). The EA 1and EA 2 groups showed a larger treatment effect than the EA 3 group. The HE results showed that EA can improve spinal histomorphology induced by neuropathic pain.CONCLUSION: EA had a strong analgesic and anti-inflammatory effect. The therapeutic effect of EA on the down-regulation of spinal EP2 m RNA expression may be greater when either local acupoints or a distal-proximal combination of acupoints are used than when distal points are used. | Ji Lili Jiang Huili Ren Xiujun Guo Mengwei Ge Dongyu Li Genmao Tu Ya | 2017 | Journal of Traditional Chinese Medicine2017，37，4： | 1 |
| 7 | Effect of electroacupuncture at distal-proximal acupoint combinations on spinal interleukin-1 beta in a rat model of neuropathic pain显示文摘Objective:Pain from herniated disc is a common type of neuropathic pain.This study investigated whether electroacupuncture (EA) stimulation at distal-proximal combinations of acupoints in the rat model of neuropathic pain modulates spinal interleukin-1 beta (IL-1β) to induce acupuncture analgesia and possibly serve as a pain-relief modality for herniated disc.Methods:A rat model of neuropathic pain was established.Rats were randomly divided into normal,model,sham,EA 1,EA 2,and EA 3 groups.EA 1 rats were needled at bilateral ExB2,BL25,BL40,and BL60 acupoints.EA 2 rats Were needled at bilateral BL40 and BL60.EA 3 rats were needled at bilateral L5 Ex-B2 and BL25.EA stimulation was administered once daily over 7 days.Mechanical withdrawal threshold from noxious mechanical stimulation was measured 1 day preoperatively and at 3,5,and7 days postoperatively.After 7 days of intervention,enzyme-linked immunosorbent assay (ELISA) was used to quantify IL-1β in the spinal cord.Results:Mechanical withdrawal threshold of rats in the model group decreased at 3 days postoperatively when compared with the normal group (P < 0.01),lasting 7 days postoperatively.Mechanical withdrawal thresholds in the EA 1,EA 2,and EA 3 groups were elevated over the model group (P < 0.05;P < 0.01).No obvious differences were found between EA 1,EA 2,and EA 3 groups.ELISA demonstrated an increase in IL-1β in the spinal cord of rats in the model group compared with the normal group (P < 0.01).EA treatment attenuated the increase in spinal IL-1β in the model group.Expression of spinal IL-1β was significantly lower in EA 1,EA 2,and EA 3 groups.Conclusion:EA at distal + proximal acupoints,distal points,as well as proximal points attenuated upregulation of spinal IL-1β,alleviated the extent of neuropathic pain hypersensitivity,and promoted mechanical withdrawal threshold,resulting in EA analgesia. | Huili Jiang Xue Yu Xiujun Ren Tingyu Fang Ya Tu | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，1： | 1 |
| 8 | GPER in CAFs regulates hypoxia-driven breast cancer invasion in a CTGF-dependentmanner显示文摘 | Juan Ren Hui Guo Huili Wu Tao Tian Danfeng Dong Yuelang Zhang Yanxia Sui Yong Zhang Dongli Zhao Shufeng Wang Zongfang Li Xiaozhi Zhang Rui Liu Jianshneg Qian Hongxia Wei Wenjun Jiang Ya Liu Yi Li | 2015 | Oncology Reports2015，，： | 1 |
| 9 | Expression, Purification, and Characterization of a Novel Soluble Form of Human Delta-like-1显示文摘 | Mei Zhao Mingyuan Wu Lingchen Guo Junfen Jiang Weiwei Huang Xiaojuan Lin Zhonghui Zhang Di Xiang Huili Lu Shunying Zhu Yan Yu Anja Moldenhauer Wei Han | 2010 | Applied Biochemistry and Biotechnology2010，，5： | 1 |
| 10 | Coseismic deformation of the 2021 M_(W)7.4 Maduo earthquake from joint inversion of InSAR, GPS, and teleseismic data显示文摘The M_(W)7.4 Maduo earthquake occurred on 22 May 2021 at 02:04 CST with a large-expansion surface rupture.This earthquake was located in the Bayan Har block at the eastern Tibetan Plateau,where eight earthquakes of M_(S)>7.0 have occurred in the past 25 years.Here,we combined interferometric synthetic aperture radar,GPS,and teleseismic data to study the coseismic slip distribution,fault geometry,and dynamic source rupture process of the Maduo earthquake.We found that the overall coseismic deformation field of the Maduo earthquake is distributed in the NWW-SEE direction along 285°.There was slight bending at the western end and two branches at the eastern end.The maximum slip is located near the eastern bending area on the northern branch of the fault system.The rupture nucleated on the Jiangcuo fault and propagated approximately 160 km along-strike in both the NWW and SEE directions.The characteristic source rupture process of the Maduo earthquake is similar to that of the 2010 M_(W)6.8 Yushu earthquake,indicating that similar earthquakes with large-expansion surface ruptures and small shallow slip deficits can occur on both the internal fault and boundary fault of the Bayan Har block. | Chaoya Liu Ling Bai Shunying Hong Yanfang Dong Yong Jiang Hongru Li Huili Zhan Zhiwen Chen | 2021 | Earthquake Science2021，34，5： | 1 |
| 11 | Preparation and characterization of multiwalled carbon nanotubes/Lyocell composite fibers显示文摘 | Lu Jiang Zhang Huzhui Shao Huili | 2007 | Polym Korea2007，31，5： | 1 |
| 12 | Concentrations and distribution of novel brominated flame retardants in the atmosphere and soil of Ny-Alesund and London Island,Svalbard,Arctic显示文摘Novel brominated flame retardants(NBFRs)were investigated in Arctic air and soil samples collected from Ny-?lesund and London Island,Svalbard,during Chinese scientific research expeditions to the Arctic during 2014–2015.The concentrations of 9 NBFRs in the Arctic air and soil were 4.9–8.7 pg/m3(average 6.8 pg/m3)and 101–201 pg/g dw(average 150 pg/g dw),respectively.The atmospheric concentration of hexabromobenzene(HBB)was significantly correlated with that of pentabromotoluene(PBT)and pentabromobenzene(PBBz),suggesting similar source and environmental fate in the Arctic air.No significant spatial difference was observed among the different sampling sites,both for air and soil samples,indicating that the effects of the scientific research stations on the occurrence of NBFRs in the Arctic were minor.The fugacities from soil to air of pentabromoethylbenzene(PBEB),2,3-dibromopropyl 2,4,6-tribromophenyl ether(DPTE),and decabromodiphenylethane 1,2-bis(pentabromophenyl)ethane(DBDPE)were lower than the equilibrium value,indicating a nonequilibrium state of these compounds between air and soil,the dominant impact of deposition and the net transport from air to soil.The correlation analysis between the measured and predicted soil-atmosphere coefficients based on the absorption model showed that the impact of the soil organic matter on the distribution of NBFRs in the Arctic region was minor.To the best of our knowledge,this work is one of the limited reports on atmospheric NBFRs in the Arctic and the first study to investigate the occurrence and fate of NBFRs in the Arctic soil. | Yanfen Hao Wenying Meng Yingming Li Xu Han Huili Lu Pu Wang Ruiqiang Yang Qinghua Zhang Guibin Jiang | 2020 | Journal of Environmental Sciences2020，32，11： | 1 |
| 13 | Treating COVID-19 with Chloroquine显示文摘A novel coronavirus disease 2019(COVID-19)emerged around December 2019 in Wuhan,China and has spread rapidly worldwide(Lu et al.,2020).Until March 27,2020,the Chinese health authorities had reported 82082 confirmed COVID-19 cases in China with 3298 deaths and 381443 confirmed cases with 20787 deaths outside China.The World Health Organization(WHO)named the virus SARS-CoV-2,which belongs to a distinct clade from the human severe acute respiratory syndrome CoV(SARS-CoV)and Middle East respiratory syndrome CoV(MERSCoV)(Zhu et al.,2020). | Mingxing Huang Tiantian Tang Pengfei Pang Man Li Ruolan Ma Jiahui Lu Jingxian Shu Yingying You Binghui Chen Jiabi Liang Zhongsi Hong Huili Chen Ling Kong Dajiang Qin Duanqing Pei Jinyu Xia Shanping jiang Hong Shan | 2020 | Journal of Molecular Cell Biology2020，12，4： | 1 |
| 14 | Effects of Short-cut Flowering Branches on Harvest Time,Yield and Quality of Guire No.82 Mango显示文摘[Objectives]To further study the technology of short-cut flowering branches for Guire No.82 Mango,adjust its harvest time,increase yield and improve fruit quality,and increase the economic benefits of mango production.[Methods]The experiment of short-cut flowering branches was carried out for Guire No.82 Mango.[Results]At the initial flowering stage and full flowering stage of the primary inflorescence,with short-cut flowering branches and corresponding cultivation techniques,Guire No.82 Mango was easy to extract regenerated inflorescences.Compared with the control group,the flowering period of the regenerated inflorescences was delayed by 30-35 d and 40-50 d,respectively;the harvest time was delayed by 30 and 40 d,respectively;the yield significantly increased by 1.63 times and 2.25 times,respectively;compared with the control group,the number of fruits with embryo increased significantly,which were 1.39 and 2.25 times of the control,respectively;there was no significant difference in the fruit quality at the harvest time.[Conclusions]Short-cut flowering branches at the initial flowering stage and full flowering stage of the primary inflorescence is an effective measure to delay the marketing time of Guire No.82 Mango. | Shudan WEI Qianfu CHEN Yong ZHONG Qiang JIANG Jie HUANG Yerong WANG Huili HUANG Jinying YANG Yuanxin CAO | 2022 | Asian Agricultural Research2022，14，7： | 0 |
| 15 | Impact of Winter Transit Air Masses on the Sources and Composition Characteristics of Atmospheric Pollution in Hengyang显示文摘Through the integration and analysis platform of particulate matter components,a preliminary comparison was conducted on the pollution contribution of the three winter transit air masses in December to environmental quality,and the changes and related relationships of water-soluble ions and particulate matter elements were analyzed.The results showed that winter transit air masses had a significant impact on the air quality in Hengyang.In the quantitative comparison of the primary pollutant contribution during the transit period of air masses,local sources and transit sources each accounted for half,and the impact of transit source on ambient air quality was much greater than that of seasonal base increase.Fine particulate matter was closely related to secondary ions,and particulate matter was closely related to primary ions.The transit of air masses promoted the improvement of secondary ion conversion rate,and the unit increment of fine particulate matter was greater than that of particulate matter.During the transit period,the mass concentrations of most water-soluble ions and most particulate matter elements maintained synchronous growth,with a superimposed effect.The composition ratio of organic carbon and nitrate ions increased,while the composition ratio of ammonia and sulfate ions decreased.Both disposable ions and secondary conversions in the composition of PM_(2.5)had increases and decreases.The high conversion rate of nitrate and the high composition ratio of ammonia radical in Hengyang indicated that transportation source factors had a significant impact on the local environmental quality of Hengyang. | Wenhui YAO Huili JIANG Chunling XU Wei ZHOU | 2023 | Meteorological and Environmental Research2023，14，3： | 0 |
| 16 | Characterization of a novel bispecific antibody targeting tissue factor-positive tumors with T cell engagement显示文摘T cell engaging bispecific antibody(TCB)is an effective immunotherapy for cancer treatment.Through co-targeting CD3 and tumor-associated antigen(TAA),TCB can redirect CD3+T cells to eliminate tumor cells regardless of the specificity of T cell receptor.Tissue factor(TF)is a TAA that involved in tumor progression.Here,we designed and characterized a novel TCB targeting TF(TF-TCB)for the treatment of TF-positive tumors.In vitro,robust T cell activation,tumor cell lysis and T cell proliferation were induced by TF-TCB.The tumor cell lysis activity was dependent upon both CD3 and TF binding moieties of the TF-TCB,and was related to TF expression level of tumor cells.In vivo,in both tumor cell/human peripheral blood mononuclear cells(PBMC)co-grafting model and established tumor models with poor T cell infiltration,tumor growth was strongly inhibited by TF-TCB.T cell infiltration into tumors was induced during the treatment.Furthermore,efficacy of TF-TCB was further improved by combination with immune checkpoint inhibitors.For the first time,our results validated the feasibility of using TF as a target for TCB and highlighted the potential for TF-TCB to demonstrate efficacy in solid tumor treatment. | Zhidi Pan Jie Chen Xiaodong Xiao Yueqing Xie Hua Jiang Baohong Zhang Huili Lu Yunsheng Yuan Lei Han Yuexian Zhou Huifang Zong Lei Wang Rui Sun Jianwei Zhu | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 0 |
| 17 | Summary of Home Nursing for Lens Dislocation Among Patients with Marfan Syndrome显示文摘Marfan syndrome is a rare genetic disease,and the condition of most patients deteriorates with age.The lesions are mainly cardiovascular,skeletal,and ocular lesions.Its clinical manifestations are characterized by congenital heart disease,ectopic lens,slender body,slender limbs,spider fingers(toes),and general muscle dysplasia.In the early stage of this disease,when the symptoms are not yet clearly manifested,some patients came to the hospital for treatment due to decreased vision.According to the particularity of crystal dislocation patients with Marfan syndrome,this article provides home nursing education in terms of physiological and psychological aspects,and builds an effective home nursing model to promote the physical and mental recovery of patients. | Jingyu He Xiaojing Liu Huili Jiang | 2023 | Journal of Clinical and Nursing Research2023，7，1： | 0 |
| 18 | Fucoidan sulfate from Sargassum fusiforme regulates the SARS-CoV-2 receptor AXL expression in human embryonic lung diploid fibroblast cells显示文摘Coronavirus disease 2019(COVID-19)is a global infectious disease that seriously endangers human life and health and affects normal social activities.Since the pandemic outbreak from December 2019 to February 2023,the total number of confirmed cases has exceeded 753 million,and the deaths caused by COVID-19 have reached 6.6 million(http://gffzze7c39cdc9e894ca6su55uk6cqubwc6f9u.ffgz.tsg.suse.edu.cn;accessed on Feb.16,2023). | Xuqiang ZHOU Weihua JIN Di JIANG Yipeng XU Sanying WANG Xinna WU Yunchuang CHANG Huili SU Tianjun ZHU Xiaogang XU Genxiang MAO | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，11： | 0 |
| 19 | N^(6)-methyladenosine demethylase ALKBH5:a novel regulator of proliferation and differentiation of chicken preadipocytes显示文摘Previous studies have reported that the N^(6)-methyladenosine demethylase ALKBH5 can regulate adipogenesis in humans.However,its function in birds remains unclear.In this study we aimed to explore the expression and function of the ALKBH5 gene in chicken adipose tissue.The results showed that ALKBH5 is widely expressed in various chicken tissues,and the expression of ALKBH5 is relatively higher in abdominal adipose tissue.In addition,the expression of ALKBH5 in abdominal adipose tissue of lean broilers is higher than that in fat broilers at 2 and 3 weeks of age.Moreover,the proliferation and differentiation of preadipocytes are associated with reduced and increased expression of ALKBH5,respectively.We also found that ALKBH5 knockout promotes preadipocyte proliferation,as evidenced by an increase in cell viability,DNA replication activity,G_(1)-S cell cycle progression,and the expressions of Ki67 and PCNA.Furthermore,after knockout of ALKBH5,the lipid droplet accumulation and the expression of PPAR_(γ),A-FABP,and FAS are reduced significantly.Thus,our results indicated that ALKBH5 is a novel regulator of proliferation and differentiation of chicken preadipocytes. | Qi Zhang Bohan Cheng Haixu Jiang Huili Zhang Hui Li | 2022 | Acta Biochimica et Biophysica Sinica2022，54，1： | 0 |
| 20 | Molecular-level effects of acupuncture on depression: a genome-wide transcriptome analysis of pituitary gland in rats exposed to chronic restraint stress显示文摘OBJECTIVE: To investigate the antidepressant mechanism of acupuncture at the molecular level in the pituitary gland of rats exposed to chronic restraint stress(CRS).METHODS: Forty-eight rats were randomly divided into a control group(C), model group(M), fluoxetine group(F), and acupuncture group(A). CRS was induced in the model group by restraining rats for 28 days. Groups F and A were given fluoxetine administration and acupuncture intervention, respectively, 1 h before the CRS procedures were implemented. On the 29 th day, rat pituitary glands were harvested for RNA extraction and RNA-sequencing analysis.RESULTS: Differentially expressed gene analysis identified 148, 87, and 179 response genes differen-tially expressed in group C vs group M, group F vs group M, and group A vs group M, respectively. Using Gene Ontology term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, we identified the up-regulation of gene sets involved in extracellular space,receptor binding, and monoamine neurotransmitter metabolism, and the down-regulation of gene sets involved in immune response and inflammatory response as the prevailing transcriptomic signatures in the pituitary gland of rats treated with CRS,fluoxetine, or acupuncture.CONCLUSION: Our findings suggest that acupuncture has a multitarget antidepressant effect at the molecular and behavioral levels. | Wang Yu Jiang Huili Meng Hong Li Jing Yang Xinjing Zhao Bingcong Yu Qiuyun Liang Xingchen Bao Tuya | 2017 | Journal of Traditional Chinese Medicine2017，37，4： | 0 |
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