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| 1 | Diagnosis of Helicobacter pylori : What should be the gold standard?显示文摘Since the discovery of Helicobacter pylori(H. pylori) in 1983, numerous detection methods for the presence of the bacterium have been developed. Each one of them has been associated with advantages and disadvantages. Noninvasive tests such as serology, 13 C urea breath test(UBT) and stool antigen tests are usually preferred by the clinicians. Serology has its own limitation especially in endemic areas while 13 C UBT is technically very demanding. The stool antigen detection method, although specific, is usually associated with poor sensitivity. The 13 C UBT is believed to be specific, but with present revelation of the fact that stomach is colonized by many other urease producing bacteria makes it questionable. Histology, culture, rapid urease test and polymerase chain reaction(PCR) are the tests which are carried out on antral biopsies collected by invasive means. Histology has been proposed to be very sensitive and specific but the question is how by simply looking the morphology of the bacteria in the microscope, one can claim that the curved bacterium is exclusively H. pylori. Rapid urease test(RUT), the doctor's test, is also challenged because the presence of other urease producing bacteria in the stomach cannot be denied. Moreover, RUT has been reported with poor sensitivity specially, when density of the bacterium is low. Isolation of H. pylori is essential to investigate its growth requirements, antibiotic susceptibility testing, studying virulence factor to develop vaccine and many more explorations. It has also got several disadvantages i.e., special condition for transporting, media, incubation and few days waiting for the colonies to appear, apart from the speed essentially needed to process the specimens. Till date, majority of the microbiological laboratories in the world are not equipped and trained to isolate such fastidious bacterium. The option left is PCR methods to detect H. pylori 's DNA in gastric mucosa, gastric juice, saliva, dental plaques and environmental specimens. There are speculations for false positivity due to detection of non-pylori Helicobacters due to genetic sharing; and false negativity due to low bacterial counts and presence of PCR inhibitors. However, specimen collection, transportation and processing do not require speed and special conditions. PCR based diagnosis may be considered as gold standard by designing primers extremely specific to H. pylori and targeting at least more than one conserved genes. Similarly specificity of PCR may be improved by use of internal Primers. Further, nested PCR will take care of false negatives by countering the effect of PCR inhibitors and low bacterial counts. Therefore, nested PCR based methods if performed properly, may be proposed as gold standard test. | Saurabh Kumar Patel Chandra Bhan Pratap Ashok Kumar Jain Anil Kumar Gulati Gopal Nath | 2014 | World Journal of Gastroenterology2014，20，36： | 30 |
| 2 | Pseudomonas fluorescens-like bacteria from the stomach:A microbiological and molecular study显示文摘AIM:To characterize oxidase-and urease-producing bacterial isolates,grown aerobically,that originated from antral biopsies of patients suffering from acid peptic diseases.METHODS:A total of 258 antral biopsy specimens were subjected to isolation of bacteria followed by tests for oxidase and urease production,acid tolerance and aerobic growth.The selected isolates were further characterized by molecular techniques viz.amplifications for 16S rRNA using universal eubacterial and HSP60 gene specific primers.The amplicons were subjected to restriction analysis and partial sequencing.A phylogenetic tree was generated using unweighted pair group method with arithmetic mean(UPGMA) from evolutionary distance computed with bootstrap test of phylogeny.Assessment of acidity tolerance of bacteria isolated from antrum was performed using hydrochloric acid from 10-7 mol/L to 10-1 mol/L.RESULTS:Of the 258 antral biopsy specimens collected from patients,179(69.4%) were positive for urease production by rapid urease test and 31%(80/258) yielded typical Helicobacter pylori(H.pylori) after 5-7 d of incubation under a microaerophilic environment.A total of 240(93%) antral biopsies yielded homogeneous semi-translucent and small colonies after overnight incubation.The partial 16S rRNA sequences revealed that the isolates had 99% similarity with Pseudomonas species.A phylogenetic tree on the basis of 16S rRNA sequences denoted that JQ927226 and JQ927227 were likely to be related to Pseudomonas fluorescens(P.fluorescens).On the basis ofHSP60 sequences applied to the UPGMA phylogenetic tree,it was observed that isolated strains in an aerobic environment were likely to be P.fluorescens,and HSP60 sequences had more discriminatory potential rather than 16S rRNA sequences.Interestingly,this bacterium was acid tolerant for hours at low pH.Further,a total of 250(96.9%) genomic DNA samples of 258 biopsy specimens and DNA from 240 bacterial isolates were positive for the 613 bp amplicons by targeting P.fluorescens-specific conserved putative outer membrane protein gene sequences.CONCLUSION:This study indicates that bacterial isolates from antral biopsies grown aerobically were P.fluorescens,and thus acid-tolerant bacteria other than H.pylori can also colonize the stomach and may be implicated in pathogenesis/protection. | Saurabh Kumar Patel Chandra Bhan Pratap Ajay Kumar Verma Ashok Kumar Jain Vinod Kumar Dixit Gopal Nath | 2013 | World Journal of Gastroenterology2013，19，7： | 4 |
| 3 | 热带地区相对湿度的季节性变化影响果蝇Drosophila jambulina体色多型性和水分平衡(英文)显示文摘在印度次大陆的亚热带地区,秋天冷而干燥,春天湿润。变温性果蝇所具有的抗干燥性有助于其度过较为干旱的气候条件。Drosophila jambulina具有体色二型性。已有研究表明,随湿度变化,D.jambulina热带种群始终保持体色多型性,这与热条件下体色黑化相反,且该热带物种中体色分化频率随季节性变化,这符合黑化-干燥假说。但是两种色型的D.jambulina产生这类气候适应的机理尚不明了。为了检验干燥相关性状生理基础的分化与对气候条件的色型特异性适应相关这一假说,我们利用分别在17℃和25℃、低湿(40%RH)和高湿(80%RH)条件下饲养获得的两种色型的D.jambulina,检测了其水分平衡对相对湿度、温度、及温湿度相互作用的反应。我们发现,在低相对湿度下,两种温度下饲养的深色型果蝇的生理和脱水性状数值显著高于浅色型。对两种色型果蝇的水分收支情况进行的比较分析表明,在低相对湿度下,深色型果蝇的含水量较高、水分损失率较低、抗脱水能力较强,使其具有更强的抗干燥性。在干燥胁迫过程中,两种色型的果蝇均以碳水化合物作为代谢燃料,但是在低湿条件下,深色果蝇中贮存碳水化合物的含量明显要高。而且,在两种湿度条件下,这两种色型果蝇之间的总能量收支显著不同。据此认为,D.jambulina的水分平衡相关性状表现出的色型特异性分化与其对湿热生境的适应相关。 | Ekta KHASA Parul BADHWAR Veer BHAN | 2013 | 昆虫学报2013，56，12： | 3 |
| 4 | Deposition of atmospheric pollutant and their chemical characterization in snow pit profile at Dokriani Glacier,Central Himalaya显示文摘The uncertainty in assessing the numerous atmospheric pollutants transported via wind from arid and semi-arid regions is affecting the glacial ecosystem. In our study area due to the complexity of the system, a prominent seasonal difference noticed among major ions(Ca^(2+), Mg^(2+), SO_4^(2-), and NO_3^-). There is a need for understanding the ions cycling as a whole and the directionality of the feedback loops in the system. Therefore, we provide an appraisal of our current hypothesis for seasonal difference in major ion concentration from snow samples for two corresponding years(2013 and 2015) at Dokriani Glacier. A systematic study of chemical compositionsin the shallow snow pit from Dokriani Glacier was undertaken for the pre-monsoon season to understand the cycling of major ions from atmosphere to solute acquisition process. The intimating connections of ions cycling in snow and its temporal behavior was observed and analyzed through various statistical tests. Among major ions, the SO_4^(2-)has the highest concentration among anions on an average considered as 14.21% in 2013 and 29.46% in 2015. On the other side Ca^(2+) is the dominant cation contributing 28.22% in 2013 and 15.3% in 2015 on average. The average ratio of Na+/Cl-was higher in 2013 whereas lower in 2015. The backward trajectory analysis suggests the possible sources of the ions transported from Central Asia through the Western Disturbance(WD) as a prominent source of winter precipitation mainly in the Central Himalaya. Ionicconcentration of Ca^(2+) in cations was highly dominated while in anion SO_4^(2-)played the major role. Factor analysis and correlation matrix suggested that, the precipitation chemistry is mostly influenced by anthropogenic, crustal, and sea salt sources over the studied region. The elemental cycling through ocean, atmosphere and biosphere opens up new ways to understand the geochemical processes operating at the glacierized catchments of the Himalaya. Moreover, increasing the field-based studies in the coming decades would also have the certain advantage in overcoming the conceptual and computational geochemical modelling difficulties. | Shipika SUNDRIYAL Tanuj SHUKLA Lekhendra TRIPATHEE Dwarika Prashad DOBHAL Sameer Kumar TIWARI Uday BHAN | 2018 | Journal of Mountain Science2018，15，10： | 2 |
| 5 | Evacuation proctography: a reappraisal of normal variability显示文摘 | S. Palit C. Bhan P. J. Lunniss D. J. Boyle M. A. Gladman C. H. Knowles S. M. Scott | 2014 | Colorectal Dis2014，，7： | 2 |
| 6 | Helicobacter pylori Is Not Eradicated after Triple Therapy: A Nested PCR Based Study显示文摘 | Saurabh Kumar Patel Girish Narayan Mishra Chandra Bhan Pratap Ashok Kumar Jain Gopal Nath Kathleen Montone | 2014 | BioMed Research International2014，，： | 2 |
| 7 | A case for regulatory B cells显示文摘 | Mizoguchi A Bhan AK | 2006 | J Immunol2006，176，2： | 1 |
| 8 | Accelerated progress to reduce under-5 mortality in India 显示文摘 | Bhan MK | 2013 | Lancent Glob Health2013，1，4： | 1 |
| 9 | Primary cementless total hip arthroplasty for bony ankylosis in patients with ankylosing spondylitis 显示文摘 | Bhan S Eachempati K K Malhotra R | 2008 | J Arthroplasty2008，23，6： | 1 |
| 10 | Colitis in transgenic and knockout animals as models of human inflammatory bowel disease显示文摘 | Bhan AK Mizoguchi E Smith RN | 1999 | Immunol Rev1999，169，： | 1 |
| 11 | Synthetic Polyurea Extreme-pressure Greases显示文摘 | SINGH T BHAN A K | | 0，，02： | 1 |
| 12 | Colitis in transgenic and knockout animal as model of human inflammatory bowel disease显示文摘 | Bhan AK Mizoguchi E Smith RN | 1999 | Immunol Rev1999，169，： | 1 |
| 13 | Sarcomatoid carcinoma: An ultrastructural study with light microscopic and immunohis tochemical correlation of 10 cases from various anatomic sites 显示文摘 | Balercia G Bhan A K Dickersin G R | 1995 | Ultrastruct Pathol1995，19，4： | 1 |
| 14 | Tissue distribution of a coelomic-epithelium-related antigen recognized by the monoclonal antibody OC125 显示文摘 | Kabawat S E Bast R C Bhan A K | 1983 | Int J Gynecol Pathol1983，2，3： | 1 |
| 15 | Antisense transcript lung noneod- ing RNA (lncRNA) HOTAIR is transcriptionally induced by estradiol 显示文摘 | Bhan A Hussain l Ansari KI | 2013 | J Mol Biol2013，425，19： | 1 |
| 16 | Primary cementless total hip arthroplasty for bony ankylosis in patients with ankylosing spondylitis显示文摘 | Bhan S Eachempati KK Malhotra R | | 0，，06： | 1 |
| 17 | Colitis in transgenic and knockout animals as models of human inflammatory bowel disease显示文摘 | BHAN A K MIZOGUCHI E SMITH R N | 1999 | Immunol Rev1999，169，4： | 1 |
| 18 | Cooperative interactions between TLR4 and TLR9 regulate interleukin 23 and 17 production in a murine model of gram negative bacterial pneu monia显示文摘 | Bhan U Ballinger MN Zeng X | 2010 | PloSOne2010，5，3： | 1 |
| 19 | Long noncoding RNAs: emerging stars in gene regulation, epigenetics and human disease显示文摘 | Bhan A Mandal SS | 2014 | Chem Med Chem2014，9，9： | 1 |
| 20 | A ease for regulatory B cells显示文摘 | Mizoguehi A Bhan AK | 2006 | J Immunol2006，176，2： | 1 |
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