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| 1 | Acute effects of rotavirus and malnutrition on intestinal barrier function in neonatal piglets显示文摘AIM: To investigate the effect of protein-energy malnutrition on intestinal barrier function during rotavirus enteritis in a piglet model.METHODS: Newborn piglets were allotted at day 4 of age to the following treatments:(1) full-strength formula(FSF)/noninfected;(2) FSF/rotavirus infected;(3) half-strength formula(HSF)/noninfected;or(4) HSF/rotavirus infected.After one day of adjustment to the feeding rates,pigs were infected with rotavirus and acute effects on growth and diarrhea were monitored for 3 d and jejunal samples were collected for Ussingchamber analyses.RESULTS: Piglets that were malnourished or infected had lower body weights on days 2 and 3 post-infection(P < 0.05).Three days post-infection,marked diarrhea and weight loss were accompanied by sharp reductions in villus height(59%) and lactase activity(91%) and increased crypt depth(21%) in infected compared with non-infected pigs(P < 0.05).Malnutrition also increased crypt depth(21%) compared to full-fed piglets.Villus:crypt ratio was reduced(67%) with viral infection.There was a trend for reduction in transepithelial electrical resistance with rotavirus infection and malnutrition(P = 0.1).3H-mannitol flux was significantly increased(50%;P < 0.001) in rotavirus-infected piglets compared to non-infected piglets,but there was no effect of nutritional status.Furthermore,rotavirus infection reduced localization of the tight junction protein,occludin,in the cell membrane and increased localization in the cytosol.CONCLUSION: Overall,malnutrition had no additive effects to rotavirus infection on intestinal barrier function at day 3 post-infection in a neonatal piglet model. | Sheila K Jacobi Adam J Moeser Anthony T Blikslager J Marc Rhoads Benjamin A Corl Robert J Harrell Jack Odle | 2013 | World Journal of Gastroenterology2013，19，31： | 4 |
| 2 | Bovine immunoglobulin protein isolates for the nutritional management of enteropathy显示文摘The gastrointestinal tract is responsible for a multitude of digestive and immune functions which depend upon the balanced interaction of the intestinal microbiota, diet, gut barrier function, and mucosal immune response. Disruptions in one or more of these factors can lead to intestinal disorders or enteropathies which are characterized by intestinal inflammation, increased gut permeability, and reduced capacity to absorb nutrients. Enteropathy is frequently associated with human immunodeficiency virus(HIV) infection, inflammatory bowel disease, autoimmune enteropathy, radiation enteritis, and irritable bowel syndrome(IBS), where pathologic changes in the intestinal tract lead to abdominal discomfort, bloating, abnormal bowel function(e.g., diarrhea, urgency, constipation and malabsorption). Unfortunately, effective therapies for the management ofenteropathy and restoring intestinal health are still not available. An accumulating body of preclinical studies has demonstrated that oral administration of plasmaor serum-derived protein concentrates containing high levels of immunoglobulins can improve weight, normalize gut barrier function, and reduce the severity of enteropathy in animal models. Recent studies in humans, using serum-derived bovine immunoglobulin/protein isolate, demonstrate that such protein preparations are safe and improve symptoms, nutritional status, and various biomarkers associated with enteropathy. Benefits have been shown in patients with HIV infection or diarrhea-predominant IBS. This review summarizes preclinical and clinical studies with plasma/serum protein concentrates and describes the effects on host nutrition, intestinal function, and markers of intestinal inflammation. It supports the concept that immunoglobulin-containing protein preparations may offer a new strategy for restoring functional homeostasis in the intestinal tract of patients with enteropathy. | Bryon W Petschow Anthony T Blikslager Eric M Weaver Joy M Campbell Javier Polo Audrey L Shaw Bruce P Burnett Gerald L Klein J Marc Rhoads | 2014 | World Journal of Gastroenterology2014，20，33： | 2 |
| 3 | Comparison of the chloride channel activator lubiprostone and the oral laxative Polyethylene Glycol 3350 on mucosal barrier repair in ischemic-injured porcine intestine显示文摘AIM: To investigate the effects of lubiprostone and Polyethylene Glycol 3350 (PEG) on mucosal barrier repair in ischemic-injured porcine intestine. METHODS: Ileum from 6 piglets (approximately 15 kg body weight) was subjected to ischemic conditions by occluding the local mesenteric circulation for 45 min in vivo. Ileal tissues from each pig were then harvested and mounted in Ussing chambers and bathed in oxygenated Ringer's solution in vitro. Intestinal barrier function was assessed by measuring transepithelial electrical resistance (TER) and mucosal-to-serosal fluxes of 3H-mannitol and 14C-inulin. Statistical analyses of data collected over a 120-min time course included 2-way ANOVA for the effects of time and treatment on indices of barrier function. RESULTS: Application of 1 μmol/L lubiprostone to the mucosal surface of ischemic-injured ileum in vitro induced significant elevations in TER compared to non-treated tissue. Lubiprostone also reduced mucosal-to-serosal fluxes of 3H-mannitol and 14C-inulin. Alternatively,application of a polyethylene laxative (PEG,20 mmol/L) to the mucosal surface of ischemic tissues significantly increased flux of 3H-mannitol and 14C-inulin. CONCLUSION: This experiment demonstrates that lubiprostone stimulates recovery of barrier function in ischemic intestinal tissues whereas the PEG laxative had deleterious effects on mucosal repair. These results suggest that,unlike osmotic laxatives,lubiprostone stimulates repair of the injured intestinal barrier. | Adam J Moeser Prashant K Nighot Birgit Roerig Ryuji Ueno Anthony T Blikslager | 2008 | World Journal of Gastroenterology2008，14，39： | 2 |
| 4 | Restoration of barrier function in injured intestinal mucosa 显示文摘 | Blikslager AT Moeser AJ Gookin JL | 2007 | Physiol Rev2007，87，2： | 1 |
| 5 | Life in the gut without oxygen: adaptive mecha- nisms and inflammatory bowel disease 显示文摘 | Blikslager AT | 2008 | Gastroenterology2008，134，1： | 1 |
| 6 | Restoration of barrier function in injured intestinal mucosa 显示文摘 | Blikslager A T Moeser A J Gookin J L | 2007 | Physiol Rev2007，87，2： | 1 |
| 7 | Glutamine and transforming growth factor-alpha stimulate extracellular regulated kinases and enhance recovery of villous surface area in porcine isehemia-injured intestine显示文摘 | Blikslager AT Rhoads JM Bristol DG | 1999 | Surgery1999，125，2： | 1 |
| 8 | Recovery of ischemic injured porcine ileum:evidence for a contributory role of COX-1 and COX-2显示文摘 | Blikslager AT、Zimmel DN、Youn KM | 2002 | Gut2002，50，： | 1 |
| 9 | Restoration of barrier function in injured intestinal mucosa 显示文摘 | Blikslager AT Moeser AJ Gookin JL | 2007 | Physiol Rev2007，87，2： | 1 |
| 10 | Glutamine metabolism stimulates intestinal cell MAPKs by a cAMP-inhibitable,Raf-independent mechanism 显示文摘 | Rhoads JM Argenzio RA Chen W Graves LM Licato LL Blikslager AT Smith J Gatzy J Brenner DA | 2000 | Gastroenterology2000，118，1： | 1 |
| 11 | Cyclooxygenase(COX)inhibitors and the intestine显示文摘 | Little D Jones SL Blikslager AT | | 0，，03： | 1 |
| 12 | Restoration of barrier function in injured intestinal mucosa显示文摘 | Blikslager A T Moeser A J Gookin J L | 2007 | Physiol Rev2007，87，2： | 1 |
| 13 | Restoration of barrier function in injured intestinal mucosa显示文摘 | Blikslager AT Moeser A J Gookin JL | 2007 | Physiol Rev2007，87，： | 1 |
| 14 | Restoration of barrier function in injured intestinal mucosa显示文摘 | Blikslager AT Moeser AJ Gookin JL | 2007 | Physiol Rev2007，87，2： | 1 |
| 15 | Determination of minimum alveolar concentration of sevoflurane in juvenile swine显示文摘 | Adam J. Moeser Anthony T. Blikslager Cliff Swanson | 2007 | Research in Veterinary Science2007，，2： | 1 |
| 16 | Effects of continuous rate intravenous infusion of butorphanol on physiologic and outcome variables in horses after celiotomy 显示文摘 | Sellon DC Roberts MC Blikslager AT | 2004 | J Intern Med2004，18，4： | 1 |
| 17 | Animal models of ischemiareperfusion-induced intestinal injury:progress and promise for translational research显示文摘 | Gonzalez LM Moeser AJ Blikslager AT | 2015 | Am J Physiol Gastrointest Liver Physiol2015，308，2： | 1 |
| 18 | Restoration of barrier function in injured intestinal mucosa 显示文摘 | Blikslager AT Moeser AJ Gookin JL | 2007 | Physiol Rev2007，87，2： | 1 |
| 19 | Glutamine and trans-forming growth factor- alpha stimulate extracellular regulated kinases and enhance recovery of villous surface area in poreine ischemia- injured intestine显示文摘 | Blikslager AT Rhoads JM Bristol DG | 1999 | Surgery1999，125，2： | 1 |
| 20 | Restoration of barrier function in injured intestinal mucosa显示文摘 | Blikslager A T Moeser A J Gookin J L | | 0，，02： | 1 |
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