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| 1 | Challenges in microRNAs’ targetome prediction and validation显示文摘MicroRNAs(miRNAs)are small RNA molecules with important roles in post-transcriptional regulation of gene expression.In recent years,the predicted number of miRNAs has skyrocketed,largely as a consequence of high-throughput sequencing technologies becoming ubiquitous.This dramatic increase in miRNA candidates poses multiple challenges in terms of data deposition,curation,and validation.Although multiple databases containing miRNA annotations and targets have been developed,ensuring data quality by validating miRNA-target interactions requires the efforts of the research community.In order to generate databases containing biologically active miRNAs,it is imperative to overcome a multitude of hurdles,including restricted miRNA expression patterns,distinct miRNA biogenesis machineries,and divergent miRNA-mRNA interaction dynamics.In the present review,we discuss recent advances and limitations in miRNA prediction,identification,and validation.Lastly,we focus on the most enriched neuronal miRNA,miR-124,and its gene regulatory network in human neurons,which has been revealed using a combined computational and experimental approach. | Jesus Eduardo Rojo Arias Volker Busskamp | 2019 | Neural Regeneration Research2019，14，10： | 3 |
| 2 | Characterizing light-regulated retinal microRNAs reveals rapid turnover as a common property of neuronal microRNAs 显示文摘 | Krol J Busskamp V Markiewicz I Stadler MB Ribi S Richter J | 2010 | Cell2010，141，4： | 1 |
| 3 | Genetic reactivation of cone photoreceptors restores visual responses in retinitis pigmentosa显示文摘 | Busskamp V Duebel J Balya D | 2010 | Science2010，329，5990： | 1 |
| 4 | Optogenetic therapy for retinitis pigmentosa 显示文摘 | Busskamp V Picaud S Sahel JA | 2012 | Gene Ther2012，19，2： | 1 |
| 5 | Characterizing light-reg- ulated retinal microRNAs reveals rapid turnover as a common property of neuronal microRNAs 显示文摘 | Krol J Busskamp V Markiewicz I | 2010 | Cell2010，141，4： | 1 |
| 6 | Genetic reactivation of cone photoreceptors restores visual responses in retinitis pigmentosa显示文摘 | Busskamp V Duebel J Balya D | 2010 | Science2010，329，5990： | 1 |
| 7 | KAP1-Mediated Epigenetic Repression in the Forebrain Modulates Behavioral Vulnerability to Stress显示文摘 | Johan Jakobsson Maria Isabel Cordero Reto Bisaz Anna C. Groner Volker Busskamp Jean-Charles Bensadoun Florence Cammas Régine Losson Isabelle M. Mansuy Carmen Sandi Didier Trono | 2008 | Neuron2008，，5： | 1 |
| 8 | Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes显示文摘Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study,repair and engineering of cardiac tissues.They are typically constructed from rigid and brittle materials processed in a cleanroom environment.An outstanding technological challenge is the integration of soft materials enabling a closer match to the mechanical properties of biological cells and tissues.Here we present an algorithm for direct writing of elastic membranes with embedded electrodes,optical waveguides and microfluidics using a commercial 3D printing system and a palette of silicone elastomers.As proof of principle,we demonstrate interfacing of cardiomyocytes derived from human induced pluripotent stem cells(hiPSCs),which are engineered to express Channelrhodopsin-2.We demonstrate electrical recording of cardiomyocyte field potentials and their concomitant modulation by optical and pharmacological stimulation delivered via the membrane.Our work contributes a simple prototyping strategy with potential applications in organ-on-chip or implantable systems that are multi-modal and mechanically soft. | Markos Athanasiadis Dzmitry Afanasenkau Wouter Derks Christoph Tondera Francesca Murganti Volker Busskamp Olaf Bergmann Ivan R.Minev | 2020 | npj Flexible Electronics2020，4，1： | 0 |
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