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| 1 | Physiological responses of osteoblasts to cyclic stretching and the change of intracellular calcium concentration显示文摘The development of bone tissues is regulated by mechanical stimulation. Cyclic stretching was applied to the osteoblasts that were delivered from rat calvarie. The results showed that stretching at 500 me increased the cell prolifera-tion while loading at 1000 me and 1500 me inhabited cell growth. Loading also increased the adhesive force between cells and substrate as well as spreading areas of osteobalsts. Furthermore, the fluorescence probe Fluo-3/AM was used to investigate the effect of stretching stimulation on the intra-cellular calcium concentration of osteoblasts. The intracellu-lar calcium concentration of osteoblasts that were stretched at 500 me for 5 min was 92.9% higher than the control. After being treated with the panax ontoginseng saponins, the stretched osteoblasts still expressed 28.6% higher intracellu-lar calcium concentration than that of the control, which proved that both the influx of extracellular calcium and the release of intracellular calcium store were involved in the increase of intracellular calcium concentration when os-teoblasts responded to the cyclic stretching. And the influx of extracellular calcium through transmembrance channel played a main role. | TANG Liling, WANG Yuanliang, GU Li, CAI Shaoxi & SU Aihua Bio-engineering College, Chongqing University, Chongqing 400044, China Correspondence should be addressed to Tang Liling | 2003 | Chinese Science Bulletin2003，48，4： | 7 |
| 2 | PDMS microchannel fabrication technique based on microwire-molding显示文摘Micro-flow channel is basic functional component of microfluidic chip,and every step-forward of its construction technique has been receiving concern all over the world.This article presents a not-complicated but flexible method for fabrication of micro-flow channels.This method mainly utilizes the conventional molding capability of polydimethylsiloxane(PDMS) and widespread commercial microwires as templates.We have fabricated out some conventional types of microchannels with different topological shapes,as examples for the demonstration of this flexible fabrication route which was not dependent on the stringent demands of photolithographical or microelectromechanical system(MEMS) techniques.The smooth surface,high-intensity,and high flexibility of the wires made it possible to create many types of topological structures of the two-dimensional or three-dimensional microchannel or channel array.The geometric shape of the cross-section of thus forming microchannel in PDMS was the negative of that of embedded-in microwire,in high-fidelity if suitable measures were taken.More-over,such a microchannel fabrication process can easily integrate the conductivity and low resistivity of the metal wire to create micro-flow devices that are suitable for the electromagnetic control of liquid or the temperature regulation in the microchannel.Furthermore some preliminary optical analysis was provided for the observation of thus forming rounded microchannel.Based on this molding strategy,we even made some prototypes for functional microflow application,such as microsolenoids chip and temperature control gadgets.And an experiment of forming a droplet in the cross channel further confirmed the feasibility and applicability of this flexible microchannel forming technique. | JIA YueFei JIANG JiaHuan MA XiaoDong LI Yuan HUANG HeMing CAI KunBao CAI ShaoXi WU YunPeng | 2008 | Chinese Science Bulletin2008，53，24： | 5 |
| 3 | A novel in vitro angiogenesis model based on a microfluidic device显示文摘Angiogenesis is very important for many physiological and pathological processes. However, the molecular mechanisms of angiogenesis are unclear. To elucidate the molecular mechanisms of angiogenesis and to develop treatments for 'angiogenesis-dependent' diseases, it is essential to establish a suitable in vitro angiogenesis model. In this study, we created a novel in vitro angiogenesis model based on a microfluidic device. Our model provides an in vivo-like microenvironment for endothelial cells (ECs) cultures and monitors the response of ECs to changes in their microenvironment in real time. To evaluate the potential of this microfluidic device for researching angiogenesis, the effects of pro-angiogenic factors on ECs proliferation, migration and tube-like structure formation were investigated. Our results showed the proliferation rate of ECs in 3D matrix was significantly promoted by the pro-angiogenic factors (with an increase of 59.12%). With the stimulation of pro-angiogenic factors gradients, ECs directionally migrated into the Matrigel from low concentrations to high concentrations and consequently formed multi-cell chords and tube-like structures. These results suggest that the device can provide a suitable platform for elucidating the mechanisms of angiogenesis and for screening pro-angiogenic or anti-angiogenic drugs for 'angiogenesis-dependent' diseases. | DAI XiaoZhen CAI ShaoXi YE QunFang JIANG JiaHuan YAN XiaoQing XIONG Xin JIANG QiFeng WANG Albert Chih-Lueh TAN Yi | 2011 | Chinese Science Bulletin2011，56，31： | 3 |
| 4 | Studying the significance of cardiac contractility variability显示文摘 | XIAO SHOUZHONG CAI SHAOXI LIU GUOCHUAN | 2000 | IEEE Engin Med Biology2000，19，3： | 1 |
| 5 | Metarhizium taii var. chongqingensis Nov., Anamorph of Cordyceps chongqingensis sp. Nov. Isolated from a Low Altitude Area in Chongqing, China显示文摘 | Yanhong Yang Shaoxi Cai Yimin Zheng Xiumin Lu Xiuying Xu Yumei Han | 2009 | Current Microbiology2009，，6： | 1 |
| 6 | Studying the significance of cardiac contractility variability显示文摘 | Cai Shaoxi Liu Guochuan | 2000 | IEEE Engineering in Medicine and Biology Magazine2000，19，3： | 1 |
| 7 | A novel MHDVdescriptor for dipeptide QSAR studies显示文摘 | LIU Shushen YIN Chunsheng CAI Shaoxi | 2001 | J Chin Chem Soc2001，48，2： | 1 |
| 8 | Studing the significance of cardiac contractility variability 显示文摘 | Xiao SZ Cai Shaoxi Liu GC | 2000 | IEEE Engineering in Medicine and Biology2000，19，3： | 1 |
| 9 | Molecular structural vector description and retention index of polycyclic aromatic hydrocar- bons 显示文摘 | LIU Shushen YIN Chunsheng CAI Shaoxi | 2002 | Chemometries and Intelligent Laboratory Systems2002，61，12： | 1 |
| 10 | Colchicine Inhibited the Expression of Tissue Inhibitor of Metalloprotenase-1 and Interleukin-6 in Cultured Activated Hepatic Stellate Cells显示文摘Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of colchicine on HSCs growth were measured by MTT assay. Effects of colchicine on gene expression of HSCs were analysed by using a self-made oligonucleotide microarray. Colchicine inhibited HSCs growth in a dose-dependent manner. After 12 h of treatment with 6.25 mg/L of colchicine, the expression of tissue inhibitor of metalloprotenase-1 (TIMP-1) and the expression of interleukin-6 (IL-6) in HSCs were down-regulated by 2.3 folds and 2.1 folds, respectively. These results suggest that colchicine’s beneficial effects may, at least in part, owe to the inhibitory to the proliferation of HSCs and down-regulation of the expression of both TIMP1 and IL-6 in HSCs. | LI Zesong CAI Shaoxi JIANG Yua GUO RuiJun ZHANG Wen | 2006 | Wuhan University Journal of Natural Sciences2006，11，3： | 1 |
| 11 | Relationship between the minute structure and the lodging resistance of rice stems显示文摘 | Duan Chuanren Wang Bochu Wang Pingqing Wang Daohong Cai Shaoxi | 2004 | Colloids and Surfaces B: Biointerfaces2004，，3： | 1 |
| 12 | Studying the significance of cardiac contractility variability 显示文摘 | Xiao Shouzhong Cai Shaoxi Liu Guochuan | 2000 | IEEE Engineering in Medicine and Biology2000，19，3： | 1 |
| 13 | Studying the significance of cardiac contractility variability显示文摘 | XIAO SHOUZHONG CAI SHAOXI LIU GUOCHUAN | 2000 | IEEE Engineering Medicine Biology2000，19，3： | 1 |
| 14 | Molecular structural vector description and retention index of polycyclic aromatic hydrocarbons显示文摘 | Shushen Liu Chunsheng Yin Shaoxi Cai | 2002 | Chemom Intell Lab Syst2002，61，： | 1 |
| 15 | The first compound heterozygosity for HKαα allele and Southeast Asian deletion allele 显示文摘 | Zesong Li Shaoxi Cai Kabin Rong | 2007 | Clinical Biochemistry2007，40，56： | 1 |
| 16 | Studying the Significance of Cardiac Contractility Variability显示文摘 | Xiao Shouzhong Cai Shaoxi Liu Guochuan | 2000 | IEEE Engineering in Medicine and Biology2000，19，3： | 1 |
| 17 | Studying the signifleanee of cardiac contractility variability显示文摘 | Xiao Shouzhong Cai Shaoxi Liu Guochuan | 2000 | IEEE Engineering in Medichm and Biology2000，19，3： | 1 |
| 18 | SDF-1B4-DCN:A NoveRecom- blnant Chimera with Dual Inhibitory Effects on Proliferation an Chemotaxis of Tumor Cells显示文摘 | Welfeng M A Shaoxi CAI Jun D U | 2008 | Biol Pharm Buli2008，31，6： | 1 |
| 19 | Expert consensus on acute exacerbation of chronic obstructive pulmonary disease in the People’s Republic of China显示文摘 | CAI Baiqiang CAI Shaoxi CHEN Rongchang | 2014 | International Journal of Chronic Obstructive Pulmonary Disease2014，，9： | 1 |
| 20 | The apoptosis of non-small cell lung cancer induced by cisplatin through modulation of STIM1显示文摘 | Wenjun Li Minhong Zhang Lei Xu Danmiao Lin Shaoxi Cai Fei Zou | 2013 | Experimental and Toxicologic Pathology2013，，： | 1 |
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