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| 1 | Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice(Oryza sativa L.)显示文摘Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the wholeplant growth duration salt tolerance(ST) was genetically dissected by phenotyping two sets of BC2F5 introgression lines(ILs) for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL(AB-QTL) analysis and selective introgression. Many QTLs affecting four yield traits under salt stress and nonstress conditions were identified, most(>90%) of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan(HHZ) ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection(MAS) or genome selection. | CHAI Lu ZHANG Jian PAN Xiao-biao ZHANG Fan ZHENG Tian-qing ZHAO Xiu-qing WANG Wen-sheng Ali Jauhar XU Jian-long LI Zhi-kang | 2014 | Journal of Integrative Agriculture2014，13，8： | 8 |
| 2 | Dry Heat Treatment Reduces the Occurrence of Cladosporium cucumerinum, Ascochyta citrullina, and Colletotrichum orbiculare on the Surface and Interior of Cucumber Seeds显示文摘Dry heat treatment has been identifie as a method for disinfecting seed-borne pathogens in vegetable seeds. This study demonstrated that three seed-borne pathogens of cucumber(Cladosporium cucumerinum that causes scabs, Ascochyta citrullina that results in gummy stem blight,and Colletotrichum orbiculare that induces anthracnose) could be effectively eradicated from cucumber seeds by dry heat treatment. In vitro growth of these three pathogens was inhibited by dry heat treatment at 70 °C for 40 min. These pathogens were inactivated after exposing infected seeds to 70 °C dry heat for at least 90 min. Seed infection was significant y reduced by exposing the seeds to 70 °C dry heat for at least 40 min. Seed moisture content and germination were slightly reduced after 70 °C heat treatment for 40–120 min. Seed vigor remained at a high level after dry heat treatment at 70 °C for 90 min. In conclusion, 70 °C dry heat treatment for 90 min was determined to be the optimal method for eradication of C. cucumerinum, Didymella bryoniae, and C. orbiculare from cucumber seeds. | SHI Yanxia MENG Shanshan XIE Xuewen CHAI Ali LI Baoju | 2016 | Horticultural Plant Journal2016，2，1： | 5 |
| 3 | Development and Evaluation of a Loop-mediated Isothermal Amplification Assay for the Rapid Detection and Identification of Pectobacterium carotovorum on Celery in the Field显示文摘Pectobacterium carotovorum is the causal agent of bacterial soft rot in a wide range of vegetable host species.Once P.carotovorum infects the plant,the spread of the disease is difficult to control.In this study,a rapid and sensitive method based on loop-mediated isothermal amplification(LAMP)was developed for detecting P.carotovorum in celery with soft rot using a primer set designed from the pmrA conserved sequence of P.carotovorum.The specificity of the LAMP primer set for P.carotovorum was extensively validated on both P.carotovorum strains and nontarget strains.The sensitivity was 1 pg of P.carotovorum genomic DNA,which demonstrated 10 times more sensitive than the conventional PCR assay.LAMP was also used to detect P.carotovorum in bacterial suspension.The lowest detection concentration was 104 CFU·mL^−1.In addition,a LAMP assay,in conjunction with a crude DNA extraction method,was successfully performed on P.carotovorum-infected samples derived from both artificially and naturally infected plants.In summary,the LAMP assay established in this study constitutes a simple,sensitive,and rapid method for the detection of P.carotovorum,and has potential application in the control of celery soft rot disease through early detection. | Yanxia Shi Zhiwen Jin Xianglong Meng Lixue Wang Xuewen Xie Ali Chai Baoju Li | 2020 | Horticultural Plant Journal2020，6，5： | 3 |
| 4 | The families Distoseptisporaceae fam.nov.,Kirschsteiniotheliaceae,Sporormiaceae and Torulaceae,with new species from freshwater in Yunnan Province,China显示文摘During a north–south latitudinal survey of aquatic fungi on submerged wood and herbaceous material in streams in the Asian region,we collected several hyphomycetous taxa.This paper is part of a series where we provide illustrated accounts of these taxa and place them in a natural classification in the fungi.DNA sequence based phylogenies in recent literature have shown that Dendryphion,Sporidesmium and Torula-like species are polyphyletic in the phylum Ascomycota and their taxonomyhas been problematic due to a lack of understanding of the importance of morphological characters used to delimit taxa,as well as the lack of ex-type or reference strains.Based on multi-locus phylogenies together with morphology,we propose the novel family Distoseptisporaceae(Sordariomycetes)and three novel genera Neotorula(Pleosporales,Dothideomycetes),Distoseptispora(Sordariomycetes)and Pseudosporidesmium(Sordariomycetes).In addition,Dendryphion aquaticum,D.submersum,Distoseptispora fluminicola,D.aquatica,Kirschsteiniothelia submersa,Neotorula aquatica,Sporidesmium aquaticum,S.submersum and S.fluminicola are introduced as new species.Pseudosporidesmium knawiae comb.nov.is proposed to accommodate Sporidesmium knawiae in Sordariomycetes.The polyphyletic nature of Dendryphion,Sporidesmium and Torula-like species are partially resolved,but further sampling with fresh collections and molecular data of species are needed to obtain a natural classification. | HongYan Su Kevin D.Hyde Sajeewa S.N.Maharachchikumbura Hiran A.Ariyawansa ZongLong Luo Itthayakorn Promputtha Qing Tian ChuanGen Lin QiuJu Shang YongChang Zhao HongMei Chai XiaoYing Liu Ali H.Bahkali Jayarama D.Bhat Eric H.C.McKenzie DeQun Zhou | 2016 | Fungal Diversity2016，，5： | 2 |
| 5 | Start-Up Characteristics of a Granule-Based Anammox UASB Reactor Seeded with Anaerobic Granular Sludge显示文摘 | Lei Xiong Yun-Yan Wang Chong-Jian Tang Li-Yuan Chai Kang-Que Xu Yu-Xia Song Mohammad Ali Ping Zheng Qaisar Mahmood | 2013 | BioMed Research International2013，，： | 1 |
| 6 | Partial nitrification in an air-lift reactor with long-term feeding of increasing ammonium concentrations显示文摘 | Chai L Y Ali M Min X B | 2015 | BiosourceTechnology2015，185，： | 1 |
| 7 | The increasing interest of ANAMMOX research in China:Bacteria, process development, and application显示文摘 | Ali M Chai L Y Tang C J | 2013 | Biomed Research International2013，，： | 1 |
| 8 | The increasing interest of anammox research in China: bacteria, process development, and application 显示文摘 | Ali M Chai LY Tang C J Zheng P Min XB Yang ZH Xiong L Song YX | 2013 | BiomedRes lnt2013，2013，： | 1 |
| 9 | Purification and characterisa- tion of an F16L mutant of a thermostable lipase显示文摘 | Mohd Shukuri Mohamad Ali Chong Chai Yun Adam Leow Thean Chor | 2012 | The Protein Journal2012，31，3： | 1 |
| 10 | Long-Term Electricity Demand Forecasting for Malaysia Using Artificial Neural Networks in the Presence of Input and Model Uncertainties显示文摘Electricity demand is also known as load in electric power system.This article presents a Long-Term Load Forecasting(LTLF)approach for Malaysia.An Artificial Neural Network(ANN)of 5-layer Multi-Layered Perceptron(MLP)structure has been designed and tested for this purpose.Uncertainties of input variables and ANN model were introduced to obtain the prediction for years 2022 to 2030.Pearson correlation was used to examine the input variables for model construction.The analysis indicates that Primary Energy Supply(PES),population,Gross Domestic Product(GDP)and temperature are strongly correlated.The forecast results by the proposed method(henceforth referred to as UQ-SNN)were compared with the results obtained by a conventional Seasonal Auto-Regressive Integrated Moving Average(SARIMA)model.The R^(2)scores for UQ-SNN and SARIMA are 0.9994 and 0.9787,respectively,indicating that UQ-SNN is more accurate in capturing the non-linearity and the underlying relationships between the input and output variables.The proposed method can be easily extended to include other input variables to increase the model complexity and is suitable for LTLF.With the available input data,UQ-SNN predicts Malaysia will consume 207.22 TWh of electricity,with standard deviation(SD)of 6.10 TWh by 2030. | Vin Cent Tai Yong Chai Tan Nor Faiza Abd Rahman Hui Xin Che Chee Ming Chia Lip Huat Saw Mohd Fozi Ali | 2021 | Energy Engineering2021，118，3： | 0 |
| 11 | Dynamic spreading characteristics of droplet impinging soybean leaves显示文摘The study on the deposition efficiency of pesticide droplets on soybean leaves can provide the basis for reducing pesticide quantity and increasing pesticide efficiency during the application of soybean plant protection machinery.The movement behavior of droplet impinges on the plant leaf surface is affected by many factors,among which the most important and the easiest to adjust are spray droplet size and impingement velocity.By changing the droplet size and impact velocity and using Fluent simulation software,the pesticide droplet hitting the soybean leaf surface was simulated and a test platform was established to verify the simulation results.The conclusions are as follows:The longitudinal roughness of soybean leaves is higher than the transverse roughness,the longitudinal pressure of soybean leaves is higher than the transverse pressure during the impact process,and the velocity of droplet spreading along the longitudinal is lower than that of spreading along the transverse;although soybean leaf surface has high adhesion,droplet losses still exist when droplet impact velocity is relatively high.The maximum spreading diameter of the droplet increases first and then decreases with the increase of impact velocity.At the same time,the maximum spreading diameter of droplet increases with the increase of particle size.The droplet deposition was best at 1.34 m/s impact velocity and 985μm particle size.This conclusion can provide optimal operation parameters for soybean plant protection operation which can be used to guide soybean plant protection operation,improve control effect,reduce quantity and increase efficiency. | He Li Xiaoxiao Niu Li Ding Ali Shahid Tahir Changle Guo Jiajun Chai Kaifei Zhang Shangshang Cheng Yiqiu Zhao Yahui Zhang Yigao Xu Zengqiang Shang | 2021 | International Journal of Agricultural and Biological Engineering2021，14，3： | 0 |
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