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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | 交联聚乙烯电缆绝缘中的导电和非导电型电树枝显示文摘研究发现,交联聚乙烯中存在导电型、非导电型和混合型三种电树枝,分别对应于不同的生长机理,主要取决于介质的聚集态和施压频率等。实验表明,均匀结晶态中的电树枝为导电型,非均匀结晶态大球晶界面的电树枝为非导电型;频率会促进非导电型电树枝的发展,但对导电型电树枝的引发与生长不产生显著影响。非导电型电树,会缓慢发展成为混合型。研究分析证实,空间电荷限制下的树枝尖端微击穿是深入到晶体中的导电型电树枝缓慢发展的主要过程,局部高温高压气体沿晶界弱区迅速膨胀、树枝通道中的局部放电和电荷复合等是非导电型电树枝发展的主要过程。 | 郑晓泉 G Chen A E Davies | 2004 | 中国电机工程学报2004，24，3： | 43 |
| 3 | 聚合物聚集态和残存应力对交联聚乙烯中电树枝的影响显示文摘实验发现了一种出现在交联聚乙烯电缆绝缘试样中的严重偏离宏观外加电场方向的电树枝单长枝结构,对试样材料的聚集态研究分析表明,这是一种受半结晶聚合物材料聚集态和材料中残存应力影响的在局部高温和高气压作用下的大球晶晶界电树枝。表明厚绝缘高压XLPE电缆绝缘在生产过程中的不均匀结晶和残存应力会对电缆绝缘的电树枝老化性能造成很大影响。 | 王以田 郑晓泉 G Chen A E Davies | 2004 | 电工技术学报2004，19，7： | 38 |
| 4 | Chinese Economic Structure and SDA Model | CHEN Xi\|kang 1,\ GUO Ju\|e 2 1. Academy of Machemation and Systems Science, Chinese Academy of Science Beijing 100080, China 2. Shaanxi Institute of Finance and Economics, Xi′an 710061, China | 2000 | Systems Science and Systems Engineering2000，10，2： | 40 |
| 5 | Molecular and cellular characterization during chondrogenic differentiation of adipose tissue-derived stromal cells in vitro and cartilage formation in vivo显示文摘 | Lin Y Luo E Chen X Liu L Qiao J Yan Z Li Z Tang W Zheng X Tian W | 2006 | 中国生物学文摘2006，20，5： | 31 |
| 6 | XLPE电缆及接头局部放电的超高频测量与分析显示文摘超高频的局部放电测量是目前国外电力设备绝缘检测研究的焦点问题之一。本文建立了一套超高频局部放电的测量系统 ,设计了XLPE电力电缆及其接头局部放电超高频测量的电容耦合传感器 ,分析了传感器的等效电路 ,测量了其频率响应 ,对电缆以及接头中的实际放电进行了测量分析。 | 徐阳 钟力生 曹晓珑 G CHEN Jason Y TIAN A E DAVIES | 2002 | 电工电能新技术2002，21，1： | 31 |
| 7 | Pharmacokinetics and disposition of anlotinib, an oral tyrosine kinase inhibitor, in experimental animal species显示文摘 | Chen-chun ZHONG Feng CHEN Jun-ling YANG Wei-wei JIA Li LI Chen CHENG Fei-fei DU Su-ping ZHANG Chengying XIE Na-ting ZHANG Olajide E OLALEYE Feng-qing WANG Fang XU Li-guang LOU Dong-ying CHEN Wei NIU Chuan LI | 2018 | Acta Pharmacologica Sinica2018，39，6： | 24 |
| 8 | XLPE电缆绝缘中的电树枝种类及其影响因素显示文摘通过大量样品实验研究,本文发现,在同等实验条件下,由于材料聚集状态的差异,在同一XLPE电缆绝缘中引发和生长的电树枝可分为枝状、丛林状、藤枝状、松枝状和混合状等五类;不同结构的电树枝对高压XLPE电缆运行可靠性的影响不同;对产生这几种电树枝结构的内在材料聚集状态特征进行了研究。 | 郑晓泉 G CHEN A E DAVIES | 2003 | 电工电能新技术2003，22，4： | 24 |
| 9 | 结晶状态对XLPE电缆绝缘中电树枝的影响显示文摘实验发现一种生长于 XL PE电缆绝缘试样中、完全不同于已发表的多数电树枝的“藤枝状”电树枝 ,从其发展方向上的突变性、扩展性和非电场依赖性结合对材料结晶状态的分析表明 ,这是一种在残存应力协同作用下生长于大晶块界面无定型区的晶界电树枝。其根本原因是大晶块界面的杂质和微孔 。 | 郑晓泉 G Chen A E Davies | 2003 | 高电压技术2003，29，11： | 22 |
| 10 | Comparison of Transformation Efficiency of piggyBac Transposon among Three Different Silkworm Bombyx mori Strains显示文摘在由编码 transposase 定序的小猪用绳子拖的平底渡船向量和助手 plasmidcontaining 的混合物的微注射编码基因进蚕鸡蛋的提高的绿荧光蛋白质(EGFP ) 的介绍以后比较的蚕 Bombyx moriwas 的三不同紧张的转变率。尽管在在在微注射鸡蛋(P=0.1258 ) 的肥沃的蛾的百分比的三紧张之中没有重要差别, G0 的百分比转变了在肥沃的蛾和注射鸡蛋的蛾两个都是显著地不同的(P=0.01368 和 P=0.02398,分别地) 。Nistari 紧张(印度紧张) 的转变率比另外的二紧张, Golden-yellow-cocoon (Vietnamesestrain ) 和 Jiaqiu (中国紧张) 的显著地高,它有类似的率。这些结果显示基于 piggyBac 的系统的转变效率可能与不同基因背景与蚕紧张变化。内长的象 piggyBac 一样元素的存在可能是影响介绍 piggyBac-derivedvectors,和多样的数量的转变效率的一个重要因素,在不同的蚕紧张的激活可能说明重要差别。 | Boxiong ZHONG~1 Jianying LI~1 Jin’e CHEN~(1,2) Jian YE~1 and Songdong YU~1 ~1College of Animal Sciences,Zhejiang University,Hangzhou 310029,China ~2Institute of Sericultural Research,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，2： | 17 |
| 11 | Dabrafenib,an inhibitor of RIP3 kinase-dependent necroptosis,reduces ischemic brain injury显示文摘Ischemic brain injury triggers neuronal cell death by apoptosis via caspase activation and by necroptosis through activation of the receptor-interacting protein kinases(RIPK) associated with the tumor necrosis factor-alpha(TNF-α)/death receptor. Recent evidence shows RIPK inhibitors are neuroprotective and alleviate ischemic brain injury in a number of animal models, however, most have not yet undergone clinical trials and safety in humans remains in question. Dabrafenib, originally identified as a B-raf inhibitor that is currently used to treat melanoma, was later revealed to be a potent RIPK3 inhibitor at micromolar concentrations. Here, we investigated whether Dabrafenib would show a similar neuroprotective effect in mice subjected to ischemic brain injury by photothrombosis. Dabrafenib administered intraperitoneally at 10 mg/kg one hour after photothrombosis-induced focal ischemic injury significantly reduced infarct lesion size in C57BL6 mice the following day, accompanied by a markedly attenuated upregulation of TNF-α. However, subsequent lower doses(5 mg/kg/day) failed to sustain this neuroprotective effect after 4 days. Dabrafenib bl ocked lipopolysaccharides-induced activation of TNF-α in bone marrow-derived macrophages, suggesting that Dabrafenib may attenuate TNF-α-induced necroptotic pathway after ischemic brain injury. Since Dabrafenib is already in clinical use for the treatment of melanoma, it might be repurposed for stroke therapy. | Shelly A.Cruz Zhaohong Qin Alexandre E R.Stewart Hsiao-Huei Chen | 2018 | Neural Regeneration Research2018，13，2： | 17 |
| 12 | Expression of calreticulin is associated with infiltration of T-cells in stage ⅢB colon cancer显示文摘AIM: To investigate the correlation between expression of calreticulin and infiltration of lymphocytes in stage ⅢB colon cancer. METHODS: Sixty-eight pathologically-confirmed speci-mens were obtained from stage ⅢB (T3N1M0) colon cancer patients who underwent radical resection between January 1999 and May 2002 at the Cancer Center of Sun Yat-Sen University, Guangzhou, China. Immuno-histochemical analysis was performed to show infiltration of lymphocytes and expression of calreticulin in colon cancer. Association between calreticulin expression, infiltration of lymphocytes, and 5-year survival rate of patients was assessed. RESULTS: The expression level of calreticulin was lower in cancer nest than in its adjacent normal epithelium since 61.8% (42/68) of the samples were stained with calreticulin in colon cancer. The expression of calreticulin in colon cancer was associated with the infi ltration of CD45RO+ cells rather than with that of CD3+ cells. In addition, the stronger expression of calreticulin and the higher infiltration of CD3+ and CD45RO+ cells in colon cancer were associated with the higher 5-year survival rate of patients. CONCLUSION: Expression of calreticulin is associated with infiltration of T-cells, which implies that a low expression level of molecular marker may represent a new mechanism underlying immune escape in colon cancer. | Rui-Qing Peng, Ya Ding, Xing Zhang, Yi-Xin Zeng, Xiao- Shi Zhang, State Key Laboratory of Oncology in South China, Biotherapy Center, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Ying-Bo Chen, Zhi-Wei Zhou, State Key Laboratory of Oncology in South China, Department of Gastric and Pancreatic Oncology, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Rong Zhang, State Key Laboratory of Oncology in South China, Department of Endoscopy, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Xing-Juan Yu, Yi-Xin Zeng, State Key Laboratory of Oncology in South China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China | 2010 | World Journal of Gastroenterology2010，16，19： | 16 |
| 13 | Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81显示文摘AIM: To further analyze the interaction of tupaia CD81 with hepatitis C virus (HCV) envelope protein E2. METHODS: A tupaia CD81 large extracellular loop (CD81 LEL), which binds to HCV E2 protein, was cloned and expressed as a GST-fusion protein, and interaction of HCV E2 protein with a tupaia CD81 LEL was evaluated by enzyme-linked immunosorbent assay (EIA). RESULTS: Although tupaia and human CD81 LEL differed in 6 amino acid changes, tupaia CD81 LEL was strongly recognized by anti-CD81 antibodies against human CD81 LEL conformation-dependent epitopes. Investigating LEL CD81-E2 interactions by EIA, we demonstrated that binding of tupaia CD81 LEL GST fusion protein to recombinant HCV E2 protein was markedly reduced compared to binding of human CD81 LEL GST fusion protein to recombinant HCV E2 protein. CONCLUSION: These data suggest that the structural differences in-between the tupaia and human CD81 may alter the interaction of the large extracellular loop with HCV envelope glycoprotein E2. These findings may be important for the understanding of the mechanisms of binding and entry of HCV to PTHs. | Zhan-Fei Tian Hong Shen Xi-Hua Fu Yi-Chun Chen Hubert E Blum Thomas F Baumert Xi-Ping Zhao | 2009 | World Journal of Gastroenterology2009，15，2： | 16 |
| 14 | Chimeric antigen receptor-engineered T-cell therapy for liver cancer显示文摘Background: Chimeric antigen receptor-engineered T-cell(CAR-T) therapy is a newly developed immunotherapy used in the treatment of cancers. Because CAR-T therapy has shown great success in treating CD19-positive hematological malignancies, its application has been explored in the treatment of solid tumors, such as liver cancer. In this review, we discuss the immune characteristics of liver cancer, the obstacles encountered during the application of CAR-T therapy, and preclinical and clinical progress in the use of CAR-T therapy in patients with liver cancer.Data sources: The data on CAR-T therapy related to liver cancers were collected by searching Pub Med and the Web of Science databases prior to December 2017 with the keywords 'chimeric antigen receptor','CAR-T', 'liver cancer', 'hepatocellular carcinoma', and 'solid tumor'. Additional articles were identified by manual search of references found in the primary articles. The data for clinical trials were collected by searching Clinical Trials.gov.Results: The liver has a tolerogenic nature in the intrahepatic milieu and its tumor microenvironment significantly affects tumor progression. The obstacles that reduce the efficacy of CAR-T therapy in solid tumors include a lack of specific tumor antigens, limited trafficking and penetration of CAR-T cells to tumor sites, and an immunosuppressive tumor microenvironment. To overcome these obstacles, several strategies have emerged. In addition, several strategies have been developed to manage the side effects of CAR-T, including enhancing the selectivity of CARs and controlling CAR-T activity. To date, no clinical trials of CAR-T therapy against HCC have been completed. However, preclinical studies in vitro and in vivo have shown potent antitumor efficacy. Glypican-3, mucin-1, epithelial cell adhesion molecule, carcinoembryonic antigen, and other targets are currently being studied.Conclusions: The application of CAR-T therapy for liver cancer is just beginning to be explored and more research is needed. However, we are optimistic that CAR-T therapy will offer a new approach for the treatment of liver cancers in the future. | Yang Chen Chang-Yong E Zhi-Wen Gong Shui Liu Zhen-Xiao Wang Yong-Sheng Yang Xue-Wen Zhang | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，4： | 16 |
| 15 | Two New Pentacyclic Triterpenes from Sabia parviflora显示文摘Two new pentacyclic triterpene acids, 1a, 3b-dihydroxyl-olean-12-en-28-oic acid and 1a, 2a, 3b-trihydroxyl-olean-12-en-28-oic acid, were isolated from the arial parts of Sabia parviflora. | Jin CHEN, Bin CHEN, Jun TIAN, Feng E WU* Laboratory of Natural Materia Medica, Chengdu Institute of Biology, Academia Sinica, Chengdu 610041 | 2002 | Chinese Chemical Letters2002，13，4： | 15 |
| 16 | Atmospheric particulate pollution of Lanzhou using magnetic measurements显示文摘To differentiate between natural and anthropogenic particulate sources in the atmosphere in Lanzhou City, samples were collected in different sites. The dust flux was calculated and magnetic measurements were conducted. Results show a distinct pattern of variation of dust flux within a year and it agrees with the shifts of atmospheric circulation regime. The magnetic parameters indicate that natural sources are the major components of atmospheric particulate during late spring and early summer, while anthropogenic sources contribute much more during winter months. The data also support the earlier findings that magnetic parameters are effective for differentiating between particulate arising from natural sources such as soil erosion and from anthropogenic sources such as coal combustion. | Chen Fa\|hu, Yang Li\|ping, Wang Jian\|min, Zhang Jia\|wu Institute of Environment & Hazards, Lanzhou University, Lanzhou 730000, China. E\|mail: fhchen@lzu.edu.cn | 1999 | Journal of Environmental Sciences1999，11，3： | 14 |
| 17 | Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat显示文摘 | Hong-Sen Bi Chen Zhang Fang-Fei Nie Bo-Lin Pan E Xiao | 2018 | Chinese Medical Journal2018，，5： | 14 |
| 18 | Overview on current criteria for heavy metals and its hint for the revision of soil environmental quality standards in China显示文摘Following rapid social and economic development over the past several decades, soil pollution by heavy metals(HMs) has been both serious and widespread in China. The Soil Environmental Quality Standards(SEQSs) in China(GB 15618-1995) have been introduced to encourage and enforce sustainable soil HM management. However, in recent years, HM contents in soils have frequently been found to exceed their associated standard values, while the crops growing on them might still meet regulatory standards, and vice versa. There is thus growing awareness that GB 15618-1995 does not effectively regulate current soil HM pollution, as it has encountered bottlenecks, and disappointing outcomes caused by poor execution along with deficiencies and gaps in the policies. However, due to the deficiency of scientific research about relationships between soil HM concentrations and their ecological or human health effects, the development of SEQSs in China is still greatly restricted. This paper discusses international SEQSs of HMs as well their development in China over time, then examines current Chinese SEQSs to demonstrate their potential regulatory deficiencies by referring to international SEQSs. The corresponding legislative policies are described, and scientific information or responses are outlined for maintaining soil environmental quality. China's experience has shown that policy and science can be linked to work in tandem to better understand and manage soil quality issues. | CHEN Shi-bao WANG Meng LI Shan-shan ZHAO Zhong-qiu E Wen-di | 2018 | Journal of Integrative Agriculture2018，17，4： | 14 |
| 19 | Quantitative Trait Loci Mapping of Maize Yield and Its Components Under Different Water Treatments at Flowering Time显示文摘干旱或水应力是导致玉米(Zea mays L.) 的一个严重农学的问题在全世界的收益损失。与干旱忍耐引起混血儿是为解决这个问题的一条重要途径。然而,更低的效率和引起混血儿的一个更长的时期是传统的繁殖节目的劣势。到传统的繁殖节目的适用的分子的标记技术能改进繁殖干旱容忍的玉米的效率,这通常被认出。在玉米干旱忍耐的研究为使用提供有用信息,为产量和它的部件印射并且标注量的特点 loci (QTL ) 根据一个混合线性模型的原则在现在的学习被执行。221 recombinant Yuyu 22 的生来的线(RIL ) 在浇得好、强调水的条件下面被种。在以前的处理组,植物很好被灌溉,而那些在 flowering 时间在后者处理组被强调。每遗传型的十植物连续不断地被种那是 3.00 m ??? 隤? ???? ? 跧?? 闦?? ? 郦 ?? ? 釧 ? ? 跧? 釧??? 雧???????? 雧? 吗?? | Gui-He Lu Ji-Hua Tang Jian-Bing Yan Xi-Qing Ma Jian-Sheng Li Shao-Jiang Chen Jian-Cang Ma Zhan-Xian Liu Li-Zhu E Yi-Rong Zhang Jing-Rui Dai | 2006 | Journal of Integrative Plant Biology2006，48，10： | 13 |
| 20 | 慢性肾脏病中的炎症和动脉硬化:慢性肾功能不全队列研究中的发现显示文摘慢性肾脏病(chronic kidney disease,CKD)和动脉硬化程度与心血管病发病率和死亡率增加相关。目前认为炎症在动脉硬化发展中发挥作用,而CKD被认为是一种促炎症反应的状态。CKD患者的动脉僵硬度增加,横断面数据提示CKD中增多的炎症标志物与较高动脉僵硬度值相关。 | Peyster E Chen J Feldman HI Go AS Gupta J Mitra N Pan Q Porter A Rahman M Raj D Reilly M Wing MR Yang W Townsend RR 陈云 叶鹏 | 2017 | 中华高血压杂志2017，25，11： | 12 |
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