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| 1 | Single-cell transcriptomic analysis defines the interplay between tumor cells,viral infection,and the microenvironment in nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is an Epstein-Barr virus(EBV)-associated malignancy with a complex tumor ecosystem.How the interplay between tumor cells,EBV,and the microenvironment contributes to NPC progression and immune evasion remains unclear.Here we performed single-cell RNA sequencing on^104,000 cells from 19 EBV^+NPCs and 7 nonmalignant nasopharyngeal biopsies,simultaneously profiling the transcriptomes of malignant cells,EBV,stromal and immune cells.Overall,we identified global upregulation of interferon responses in the multicellular ecosystem of NPC.Notably,an epithelial–immune dual feature of malignant cells was discovered and associated with poor prognosis.Functional experiments revealed that tumor cells with this dual feature exhibited a higher capacity for tumorigenesis.Further characterization of the cellular components of the tumor microenvironment(TME)and their interactions with tumor cells revealed that the dual feature of tumor cells was positively correlated with the expression of co-inhibitory receptors on CD8^+tumor-infiltrating T cells.In addition,tumor cells with the dual feature were found to repress IFN-γproduction by T cells,demonstrating their capacity for immune suppression.Our results provide new insights into the multicellular ecosystem of NPC and offer important clinical implications. | Shanzhao Jin Ruoyan Li Ming-Yuan Chen Chao Yu Lin-Quan Tang Yan-Min Liu Jiang-Ping Li Yi-Na Liu Yi-Ling Luo Yifan Zhao Yu Zhang Tian-Liang Xia Shang-Xin Liu Qi Liu Guan-Nan Wang Rui You Jing-Yun Peng Jiang Li Feng Han Jianwei Wang Qiu-Yan Chen Li Zhang Hai-Qiang Mai Benjamin E.Gewurz Bo Zhao Lawrence S.Young Qian Zhong Fan Bai Mu-Sheng Zeng | 2020 | Cell Research2020，30，11： | 16 |
| 2 | Stationarity Intervals of Time-Variant Channel in High Speed Railway Scenario显示文摘The non-stationary behavior, caused by the train movement, is the main factor for the variation of high speed railway channel. To measure the time-variant effect, the parameter of stationarity interval, in which the channel keeps constant or has no great change, is adopted based on Zhengzhou-Xi'an (Zhengxi) passenger dedicated line measurement with different train speeds. The stationarity interval is calculated through the definition of Local Region of Stationarity (LRS) under three train velocities. Furthermore, the time non-stationary characteristic of high speed railway channel is compared with five standard Multiple-Input Multiple-Output (MIMO) channel models, i.e. Spatial Channel Model (SCM), extended version of SCM (SCME), Wireless World Initiative New Radio Phase II (WINNERII), International Mobile Telecommunications-Advanced (IMT-Advanced) and WiMAX models which contain the high speed moving scenario. The stationarity interval of real channel is 9 ms in 80% of the cases, which is shorter than those of the standard models. Hence the real channel of high speed railway changes more rapidly. The stationarity intervals of standard models are different due to different modeling methods and scenario definitions. And the compared results are instructive for wireless system design in high speed railway. | Chen Binghao Zhong Zhangdui Ai Bo | 2012 | China Communications2012，9，8： | 15 |
| 3 | Minimizing tacrolimus decreases the risk of new-onset diabetes mellitus after liver transplantation显示文摘AbstractAIM: To investigate the impact of minimum tacrolimus(TAC) on new-onset diabetes mellitus (NODM) afterliver transplantation (LT).METHODS: We retrospectively analyzed the data of973 liver transplant recipients between March 1999and September 2014 in West China Hospital LiverTransplantation Center. Following the exclusion ofineligible recipients, 528 recipients with a TAC-dominantregimen were included in our study. We calculatedand determined the mean trough concentration ofTAC (cTAC) in the year of diabetes diagnosis in NODMrecipients or in the last year of the follow-up in non-NODM recipients. A cutoff of mean cTAC value forpredicting NODM 6 mo after LT was identified usinga receptor operating characteristic curve. TAC-relatedcomplications after LT was evaluated by χ^2 test, andthe overall and allograft survival was evaluated usingthe Kaplan-Meier method. Risk factors for NODM afterLT were examined by univariate and multivariate Cox regression.RESULTS: Of the 528 transplant recipients, 131(24.8%) developed NODM after 6 mo after LT, andthe cumulative incidence of NODM progressivelyincreased. The mean cTAC of NODM group recipientswas significantly higher than that of recipients in thenon-NODM group (7.66 ± 3.41 ng/mL vs 4.47 ± 2.22ng/mL, P 〈 0.05). Furthermore, NODM group recipientshad lower 1-, 5-, 10-year overall survival rates (86.7%,71.3%, and 61.1% vs 94.7%, 86.1%, and 83.7%, P 〈0.05) and allograft survival rates (92.8%, 84.6%, and75.7% vs 96.1%, 91%, and 86.1%, P 〈 0.05) thanthe others. The best cutoff of mean cTAC for predictingNODM was 5.89 ng/mL after 6 mo after LT. Multivariateanalysis showed that old age at the time of LT (〉 50years), hypertension pre-LT, and high mean cTAC (≥5.89 ng/mL) after 6 mo after LT were independent riskfactors for developing NODM. Concurrently, recipientswith a low cTAC (〈 5.89 ng/mL) were less likely tobecome obese (21.3% vs 30.2%, P 〈 0.05) or todevelop dyslipidemia (27.5% vs 44.8%, P 〈0.05),chronic kidney dysfunction (14.6% vs 22.7%, P 〈 0.05),and moderate to severe infection (24.7% vs 33.1%, P〈 0.05) after LT than recipients in the high mean cTACgroup. However, the two groups showed no significantdifference in the incidence of acute and chronicrejection, hypertension, cardiovascular events and newonsetmalignancy.CONCLUSION: A minimal TAC regimen can decreasethe risk of long-term NODM after LT. Maintaining a cTACvalue below 5.89 ng/mL after LT is safe and beneficial. | Jiu-Lin Song Wei Gao Yan Zhong Lu-Nan Yan Jia-Yin Yang Tian-Fu Wen Bo Li Wen-Tao Wang Hong Wu Ming-Qing Xu Zhe-Yu Chen Yong-Gang Wei Li Jiang Jian Yang | 2016 | World Journal of Gastroenterology2016，22，6： | 12 |
| 4 | Effect of oxymatrine on specific cytotoxic T lymphocyte surface programmed death receptor-1 expression in patients with chronic hepatitis B显示文摘 | GU Xi-bing YANG Xiao-juan HUA Zhong LU Zhong-hua ZHANG Bo ZHU Yin-fang WU Hang-yuan JIANG Yi-ming CHEN Hao-kun PEI Hao | 2012 | Chinese Medical Journal2012，，8： | 11 |
| 5 | A multiplex PCR for differential detection of Echinococcus granulosus sensu stricto,Echinococcus multilocularis and Echinococcus canadensis in China显示文摘Background:Echinococcosis caused byEchinococcus is one of the most major infectious diseases in north-west highland of China.E.granulosus sensu strict,E.multilocularis,andE.canadensis are known to be the only three species related to human health transmitting in the areas.To achieve targeted treatment and control of echinococcosis,the accurate identification and discrimination of the species are important.However,currently the available diagnostic approaches do not present ideal results either in accuracy or efficiency.Methods:In the study,a set of primers were designed to aim at the three human-pathogenicEchinococcus species in China.The one-step multiplex PCR assay was developed and evaluated for the specificity and sensitivity.A total of 73parasitic lesions and 41 fecal materials obtained from human and various animals collected in the clinic and the field were tested to assess the applicability of this method.Results:The multiplex PCR effectively detected the individual DNA from the targeted species and their random mixtures generating with distinguishable expected size of products.The detection limit of the assay for each of the three species was 5 pg/μl when they were tested separately.When DNA mixtures of the targeted species containing the same concentration were used as templates,the lowest amount of DNA which can be detected was 50 pg/μl,10 pg/μl and 5 pg/μl forE.granulosus s.s.,E.multilocularis,andE.canadensis respectively.No cross-reactivity was observed when DNA from eight genetically close species was used as control templates.The multiplex PCR identifications of all samples were in line with the original sequencing results except for those infected withE.shiquicus,which showed negative signals in the developed assay.Of all the tested stool materials,16 were previously found positive forEchinococcus by visual and microscopic examination.Among these 16 samples,13 were confirmed by the multiplex PCR,and the other three tested negative.Additionally,the multiplex PCR identified another 14 positive feces from the remained 25 stool samples which absence of worms.Conclusions:The developed multiplex PCR shows advantages in fast diagnosis and large-scale epidemiological investigation,which proven to be a promising tool utilized in clinic and surveillance system. | Jing-Ye Shang Guang-Jia Zhang Sha Liao Yan Huang Wen-Jie Yu Wei He Guang-You Yang Tiao-Ying Li Xing-Wang Chen Bo Zhong Qian Wang Qi Wang Rui-Rui Li Hao Wang | 2019 | Infectious Diseases of Poverty2019，8，4： | 11 |
| 6 | Targeted Mutagenesis of NAC Transcription Factor Gene,OsN AC041,Leading to Salt Sensitivity in Rice显示文摘Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC(NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes(DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotictype ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding. | WANG Bo ZHONG Zhaohui ZHANG Huanhuan WANG Xia LIU Binglin YANG Lijia HAN Xiangyan YU Deshui ZHENG Xuelian WANG Chunguo SONG Wenqin CHEN Chengbin ZHANG Yong | 2019 | Rice science2019，26，2： | 8 |
| 7 | Influence of dosage forms on pharmacokinetics of daidzein and its main metabolite daidzein-7-O-glucuronide in rats显示文摘瞄准:在 Wistar 老鼠在黄豆甙原和它的主要代谢物 daidzein-7-O-glucuronide 的 pharmacokinetics 上调查剂量表格的影响。方法:在二张典型剂量表格(黄豆甙原答案和暂停) 的管理以后,黄豆甙原和 daidzein-7-O-glucuronide 的集中被一个 LC-MS-MS 方法决定。pharmacokinetic 参数用学生的 t 测试统计上计算、分析。结果:在黄豆甙原答案(tmax=0.46 h ) 的管理以后的黄豆甙原的吸收比暂停(tmax=5.00 h ) 的更快速。分别地,在黄豆甙原答案和暂停的管理以后的黄豆甙原的山峰血浆集中是 601.1 microg/L 和 127.3 microg/L, daidzein-7-O-glucuronide 的那些分别地是 3000 microg/L 和 192.6 microg/L。分别地,在在黄豆甙原答案和暂停的管理以后的老鼠的免费黄豆甙原的绝对简历可获得性是 12.8% 和 6.1% 它是打算的分别地,在全部的黄豆甙原(免费正结合的黄豆甙原) 形式是 47.0% 和 12.2% 。 | Feng QIU Xiao-yan CHEN Bo SONG Da-fang ZHONG Chang-xiao LIU | 2005 | Acta Pharmacologica Sinica2005，26，9： | 7 |
| 8 | The ubiquitin-specific protease 17 is involved in virus-triggered type I IFN signaling显示文摘病毒的感染开始激活抄写因素的一系列发信号的串联原子因素 kappa B 和干扰素规章的因素 3,它协作为类型导致基因的抄写我干扰素(IFN ) 和另外的 cytokines。这里,我们报导 deubiquitinating 酶 ubiquitin 特定的朊酶(USP17 ) 17 为导致病毒的 RIG-I- 和黑瘤被要求联系区别的 protein-5 (MDA5 ) 调停了类型我 IFN 发信号。内长的 USP17 击倒禁止的病毒 -- ,细胞质 poly (我: C )- 并且(dA:dT ) poly 导致了 IFN- 尾倡导者和细胞的抗病毒的回答的激活。我们进一步 USP17 发现那击倒禁止了 RIG-I- 和 IFN- 尾倡导者的导致 MDA5 然而并非下游的导致使活跃之物的激活,它随 RIG-I 和 MDA5 的 ubiquitination 层次的增加被相关。一起拿,我们的调查结果建议 USP17 通过 RIG-I 和 MDA5 的 deubiquitination 工作调整导致病毒的类型我 IFN 发信号。 | Rui Chen Lu Zhang Bo Zhong Bo Tan Yu Liu Hong-Bing Shu | 2010 | Cell Research2010，20，7： | 6 |
| 9 | Generation of Febrile Seizures and Subsequent Epileptogenesis显示文摘Febrile seizures(FSs) occur commonly in children aged from 6 months to 5 years. Complex(repetitive or prolonged) FSs, but not simple FSs, can lead to permanent brain modification. Human infants and immature rodents that have experienced complex FSs have a high risk of subsequent temporal lobe epilepsy. However, the causes of FSs and the mechanisms underlying the subsequent epileptogenesis remain unknown. Here, we mainly focus on two major questions concerning FSs: how fever triggers seizures, and how epileptogenesis occurs after FSs. The risk factors responsible for the occurrence of FSs and the epileptogenesis after prolonged FSs are thoroughly summarized and discussed. An understanding of these factors can provide potential therapeutic targets for the prevention of FSs and also yield biomarkers for identifying patients at risk of epileptogenesis following FSs. | Bo Feng Zhong Chen | 2016 | Neuroscience Bulletin2016，32，5： | 6 |
| 10 | Aggregation-induced Emission-active Hyperbranched Poly (tetrahydropyrimidine)s Synthesized from Multicomponent Tandem Polymerization显示文摘Hyperbranched polymer with highly branched three-dimensional topological structure, a large number of end groups, and multifaceted functionalities have gained much attention, while polymers with aggregation-induced emission(AIE) properties become a group of popular luminescent materials recently. The design and synthesis of AIE-active hyperbranched polymers, which combine the advantages of these two types of materials, are attractive but challenging. In this work, four hyperbranched poly(tetrahydropyrimidine)s were synthesized from the metal-free room temperature multicomponent tandem polymerization of diester group-activated internal alkyne,polyfunctional aromatic amines, and formaldehyde in methanol under the catalysis of acetic acid. Through different monomer combination and controlling the monomer loading order, hyperbranched polymers with various topological structures as well as sequences of different functional groups in the polymer backbone were obtained with high molecular weights(up to 3.0 × 10~4 g/mol) in high yields(up to 98%). The hyperbranched poly(tetrahydropyrimidine) emitted faintly in solution, while its luminescence was notably enhanced in the aggregated state, suggesting its typical aggregation-induced emission property. It is anticipated that the multicomponent polymerization may provide a synthetic platform for the construction of hyperbranched polyheterocycles with diverse structures and functionalities. | Yuzhang Huang Ping Chen Bo Wei Rongrong Hu Ben Zhong Tang | 2019 | Chinese Journal of Polymer Science2019，37，4： | 6 |
| 11 | Lipid nanoparticle-encapsulated mRNA antibody provides longterm protection against SARS-CoV-2 in mice and hamsters显示文摘Monoclonal antibodies represent important weapons in our arsenal to against the CO VID-19 pandemic.However,this potential is severely limited by the time-consuming process of developing effective antibodies and the relative high cost of manufacturing.Herein,we present a rapid and cost-effective lipid nanoparticle(LNP)encapsulated-mRNA platform for in vivo delivery of SARS-CoV-2 neutralization antibodies.Two mRNAs encoding the light and heavy chains of a potent SARS-CoV-2 neutralizing antibody HB27,which is currently being evaluated in clinical trials,were encapsulated into clinical grade LNP formulations(named as mRNA-HB27-LNP).I n vivo characterizatio n dem on strated that intravenous administratio n of mRNA-HB27-LNP in mice resulted in a longer circulating half-life compared with the original HB27 antibody in protein format.More importantly,a single prophylactic administration of mRNA-HB27-LNP provided protection against SARS-CoV-2 challenge in mice at 1,7 and even 63 days post administration.In a close contact transmission model,prophylactic administration of mRNA-HB27-LNP prevented SARS-CoV-2 in fecti on betwee n hamsters in a dose-depende nt mariner.Overall,our results dem on strate a superior Ion g-term protect!on agai nst SARS-CoV-2 conferred by a single administration of this unique mRNA antibody,highlight!ng the potential of this universal platform for antibody-based disease prevention and therapy against COVID-19 as well as a variety of other infectious diseases. | Yong-Qiang Deng Na-Na Zhang Yi-Fei Zhang Xia Zhong Sue Xu Hong-Ying Qiu Tie-Cheng Wang Hui Zhao Chao Zhou Shu-Long Zu Qi Chen Tian-Shu Cao Qing Ye Hang Chi Xiang-Hui Duan Dan-Dan Lin Xiao-Jing Zhang Liang-Zhi Xie Yu-Wei Gao Bo Ying Cheng-Feng Qin | 2022 | Cell Research2022，32，4： | 5 |
| 12 | Tea Consumption is Associated with Increased Risk of Kidney Stones in Northern Chinese: A Cross-sectional Study显示文摘Kidney stones are a common urinary system condition that can progress to kidney disease.Previous studies on the association between tea consumption and kidney stones are inconsistent.A cross‐sectional study to investigate the association between tea consumption and kidney stones was conducted from 2013 to 2014 and recruited 9,078northern Chinese adults.A total of 8,807participants were included in the final analysis. | WU Zhong Biao JIANG Tian LIN Guo Bing WANG You Xin ZHOU Yong CHEN Zhen Qian XU Yong Ming YE Hai Bo CHEN Bo Jun BAO Xiao Zhao ZHANG Cun Ming | 2017 | Biomedical and Environmental Sciences2017，30，12： | 5 |
| 13 | Curcumin inhibits the replication of enterovirus 71 in vitro显示文摘Human enterovirus 71(EV71)is the main causative pathogen of hand,foot,and mouth disease(HFMD)in children.The epidemic of HFMD has been a public health problem in Asia-Pacific region for decades,and no vaccine and effective antiviral medicine are available.Curcumin has been used as a traditional medicine for centuries to treat a diversity of disorders including viral infections.In this study,we demonstrated that curcumin showed potent antiviral effect again EV71.In Vero cells infected with EV71,the addition of curcumin significantly suppressed the synthesis of viral RNA,the expression of viral protein,and the overall production of viral progeny.Similar with the previous reports,curcumin reduced the production of ROS induced by viral infection.However,the antioxidant property of curcumin did not contribute to its antiviral activity,since N-acetyl-L-cysteine,the potent antioxidant failed to suppress viral replication.This study also showed that extracellular signal-regulated kinase(ERK)was activated by either viral infection or curcumin treatment,but the activated ERK did not interfere with the antiviral effect of curcumin,indicating ERK is not involved in the antiviral mechanism of curcumin.Unlike the previous reports that curcumin inhibited protein degradation through ubiquitin–proteasome system(UPS),we found that curcumin had no impact on UPS in control cells.However,curcumin did reduce the activity of proteasomes which was increased by viral infection.In addition,the accumulation of the short-lived proteins,p53 and p21,was increased by the treatment of curcumin in EV71-infected cells.We further probed the antiviral mechanism of curcumin by examining the expression of GBF1 and PI4KB,both of which are required for the formation of viral replication complex.We found that curcumin significantly reduced the level of both proteins.Moreover,the decreased expression of either GBF1 or PI4KB by the application of siRNAs was sufficient to suppress viral replication.We also demonstrated that curcumin showed anti-apoptotic activity at the early stage of viral infection.The results of this study provide solid evidence that curcumin has potent anti-EV71 activity.Whether or not the down-regulated GBF1 and PI4KB by curcumin contribute to its antiviral effect needs further studies. | Ying Qin Lexun Lin Yang Chen Shuo Wu Xiaoning Si Heng Wu Xia Zhai Yan Wang Lei Tong Bo Pan Xiaoyan Zhong Tianying Wang Wenran Zhao Zhaohua Zhong | 2014 | Acta Pharmaceutica Sinica B2014，4，4： | 5 |
| 14 | Imaging energetic electron spectrometer onboard a Chinese navigation satellite in the inclined GEO orbit显示文摘The energetic electron measurement is one of the most important issues to understand dynamics in space physics and the applications for space weather. In this study, the principle and functional components of the imaging energetic electron spectrometer(IES) onboard a Chinese navigation satellite in the inclined GEO orbit(IGSO) was introduced. The IES instrument is developed by the team in Peking University(BeiDa), thus it is named as BD-IES. Based on the pin-hole technique, the instrument can measure 50–600 keV electrons incident from 9 directions over a range of 180° in polar angle. With pulse height analysis(PHA), the spectrum can be determined for each direction. The energy and angular calibrations were performed, which show the good energy and angular characteristics of BD-IES. Monte Carlo simulations show that the anti-proton design of BDIES can effectively decrease the proton contamination on the electron measurements in the inclined GEO orbit. The primary results of BD-IES verify the successful design of this instrument. | ZOU Hong YE YuGuang ZONG QiuGang CHEN HongFei ZOU JiQing CHEN Jiang SHI WeiHong YU XiangQian ZHONG WeiYing WANG YongFu ZHOU XuZhi HAO YiXin CHEN XingRan JIA XiangHong XU Feng SHAO SiPei WANG Bo HAO XiaoYun ZHANG XiaoXin | 2018 | Science China(Technological Sciences)2018，61，12： | 5 |
| 15 | Genome-wide analysis identified 17 new loci influencing intraocular pressure in Chinese population显示文摘Intraocular pressure(IOP) is a major risk factor for glaucoma. Genetic determinants of intraocular pressure can provide critical insights into the genetic architecture of glaucoma and, as a result, open new avenues for therapeutic intervention. We performed a genome-wide association study and replication analysis of 8,552 Chinese participants. In the genome-wide association study, we identified 51 loci that surpassed the significance of P<9×10^(-7), and we formally replicated these loci. A combined discovery and replication meta-analysis identified 21 genome-wide loci that surpassed the genome-wide significance of P<5×10^(-8), including 4 previously reported loci: rs145063132(7 p21.2, ETV1/DGKB), rs548030386(7 q31.2, ST7 near CAV1/CAV2), rs7047871(9 p24.2, GLIS3), and rs2472494(9 q31.1, ABCA1/SLC44 A1). Of the 17 newly identified loci, five were reported to have ocular related phenotypes: PTCH2(rs7525308 in 1 p34.1), LRIF1/DRAM2(rs1282146 in 1 p13.3), COLEC11(rs201143466 in 2 p25.3),SPTBN1(rs4514918 in 2 p16.2), and CRK(rs11078446 in 17 p13.3). The genetic loci identified in this study not only increase our understanding of the genes involved in intraocular pressure but also provide important genetic markers to improve future genetic screening and drug discovery for intraocular pressure disorders. | Lulin Huang Yuhong Chen Ying Lin Pancy O.S.Tam Yilian Cheng Yi Shi Bo Gong Fang Lu Jialiang Yang Haixin Wang Yi Yin Yong Cao Dan Jiang Ling Zhong Bai Xue Jing Wang Fang Hao Dean-Yao Lee Chi-Pui Pang Xinghuai Sun Zhenglin Yang | 2019 | Science China(Life Sciences)2019，62，2： | 4 |
| 16 | Human epidermal growth factor receptor 2 expression in mixed gastric carcinoma显示文摘AIM: To investigate human epidermal growth factor receptor 2(HER2) amplification and protein expression in mixed gastric carcinoma.METHODS: Fluorescence in situ hybridization and immunohistochemistry were used to detect HER2 amplification and protein expression in 277 cases of mixed gastric carcinoma. Protein staining intensity was rate as 1+, 2+, or 3+.RESULTS: Of the 277 cases, 114(41.2%) expressed HER2 protein. HER2 3+ staining was observed in 28/277(10.1%) cases, 2+ in 37/277(13.4%) cases, and 1+ in 49/277(17.7%) cases. A HER2 amplification rate of 17% was detected, of which 25/28(89.3%) were observed in the HER2 3+ staining group, 17/37(45.9%) in 2+, and 5/49(10.2%) in 1+. Of the 47 patients with HER2 amplification who received chemotherapy plus trastuzumab, 22 demonstrated median progression-free and overall survivals of 9.1 mo and 16.7 mo, respectively, which were significantly better than those achieved with chemotherapy alone(5.6 mo and 12.1 mo, respectively) in 19 previously treated patients(P s < 0.05). CONCLUSION: HER2 detection in mixed gastric carcinoma displays high heterogeneity. Relativelyquantitative parameters are needed for assessing the level of HER2 amplification and protein expression. | Yang-Kun Wang Zhong Chen Tian Yun Cong-Yang Li Bo Jiang Xue-Xia Lv Guang-Hui Chu Su-Nan Wang Hui Yan Lei-Feng Shi | 2015 | World Journal of Gastroenterology2015，21，15： | 4 |
| 17 | Survival difference between EGFR Del19 and L858R mutant advanced non-small cell lung cancer patients receiving gefitinib:a propensity score matching analysis显示文摘Objective: Although superior clinical benefits of epidermal growth factor receptor(EGFR) tyrosine kinase inhibitors(TKIs) in the treatment of advanced non-small-cell lung cancer(NSCLC) had been reported,the survival difference between exon 19 deletion(Del19) and exon 21 Leu858 Arg substitution(L858 R) remains controversial.The purpose of this study is to investigate the differences in progression-free survival(PFS) and overall survival(OS) between different EGFR mutant subtypes among advanced NSCLC patients receiving gefitinib.Methods: There were 204 advanced NSCLC patients with EGFR mutations treated with gefitinib were enrolled in this retrospective cohort study.Patients were divided into the EGFR Del19 group and the L858 R mutated group according to their mutant subtype.Propensity score matching(PSM) was conducted by using a nearest-neighbor algorithm(1:1) to adjust for demographical and clinical covariates.Survival curves were constructed with the Kaplan-Meier method and compared by using the log-rank test.Results: The PFS in Del19 group was similar to that in the L858 R group [before PSM 8.6 vs.7.2 months,P=0.072; after PSM 7.3 vs.7.2 months,P=0.155].No differences were detected in OS between the L858 R and the Del19 group(before PSM 17.8 vs.13.1 months,P=0.253; after PSM 16.9 vs.13.1 months,P=0.339).The Del19 group was significantly younger compared with the L858 R mutation group in age(P=0.015).Conclusions: No significant difference was found in the PFS or OS between the Del19 and L858 R mutant NSCLC patients receiving gefitinib.The age gap might contribute to the survival differences between Del19 and L858 R groups.PSM is of important value to the elimination of potential bias. | Minglei Zhuo Qiwen Zheng Jun Zhao Meina Wu Tongtong An Yuyan Wang Jianjie Li Shuhang Wang Jia Zhong Xue Yang Hanxiao Chen Bo Jia Zhi Dong Emei Gao JingjingWang Ziping Wang | 2017 | Chinese Journal of Cancer Research2017，29，6： | 4 |
| 18 | Crack status analysis for concrete dams based on measured entropy显示文摘The integrity and safety of concrete dams are seriously affected by the existing cracks in dam bodies, and some serious cracks may cause dam failure or disaster. The propagation of cracks in concrete dams is accompanied by changes in energy distribution, which can be represented by changes in the structure's system entropy. Therefore, the entropy theory can be used in analyzing the behavior of dam cracks. Due to the randomness and locality of crack propagation, it is difficult to predict the location of cracks by traditional monitoring methods. To solve this problem, the influence of spatial positions of monitoring points on inspection zones is represented by a weight index, and the weight index is determined by the distance measure method proposed in this paper. Through the weighted linear fusion method, the entropy of multiple monitoring points is obtained for analyzing the behavior of dam cracks in the selected zones. Meanwhile, the catastrophe theory is used as the variation criterion of an entropy sequence in order to predict the instability time of dam cracks. Case studies are put forward on a high arch dam, and the fusion entropy is calculated according to the monitoring data from strain gauges. Results show that the proposed method can effectively predict the occurrence time and location of dam cracks regardless of the layout of monitoring instruments, and it is a new way to analyze the occurrence and propagation of dam cracks. | WU Bang Bin WU Zhong Ru CHEN Bo SU Huai Zhi BAO Teng Fei WANG Shao Wei | 2016 | Science China(Technological Sciences)2016，59，5： | 3 |
| 19 | Determination of chlorogenic acid by flow injection irreversible biamperometry显示文摘A flow injection irreversible biamperometric method for the determination of chlorogenic acid is described.The proposedmethod is based on the electrochemical oxidation of chlorogenic acid at pretreated platinum electrode and the reduction ofpermanganate at another electrode to form an irreversible biamperometric detection system.Under the external potential difference(△E)of 0 V,in the 0.05 mol/L sulfuric acid,chlorogenic acid can be determined over the range 0.8-120 mg/L with a samplemeasurement frequency of 80 samples/h.The detection limit is 0.18 mg/L.The proposed method exhibits the satisfactoryreproducibility with a relative standard derivation(R.S.D.)of 2.21%for 19 successive determinations of 40 mg/L. | Li Jun Li Qi Feng Chen Lai Bo Yu Zhao Heng Zhong Jun Feng Hao Cheng Hong Xing Kong Jian Ling Wu | 2007 | Chinese Chemical Letters2007，18，4： | 3 |
| 20 | Deep learning for automatically predicting early haematoma expansion in Chinese patients显示文摘Background and purpose Early haematoma expansion is determinative in predicting outcome of intracerebral haemorrhage(ICH)patients.The aims of this study are to develop a novel prediction model for haematoma expansion by applying deep learning model and validate its prediction accuracy.Methods Data of this study were obtained from a prospectively enrolled cohort of patients with primary supratentorial ICH from our centre.We developed a deep learning model to predict haematoma expansion and compared its performance with conventional non-contrast CT(NCCT)markers.To evaluate the predictability of this model,it was also compared with a logistic regression model based on haematoma volume or the BAT score.Results A total of 266 patients were finally included for analysis,and 74(27.8%)of them experienced early haematoma expansion.The deep learning model exhibited highest C statistic as 0.80,compared with 0.64,0.65,0.51,0.58 and 0.55 for hypodensities,black hole sign,blend sign,fluid level and irregular shape,respectively.While the C statistics for swirl sign(0.70;p=0.211)and heterogenous density(0.70;p=0.141)were not significantly higher than that of the deep learning model.Moreover,the predictive value for the deep learning model was significantly superior to that of the logistic model of haematoma volume(0.62;p=0.042)and the BAT score(0.65;p=0.042).Conclusions Compared with the conventional NCCT markers and BAT predictive model,the deep learning algorithm showed superiority for predicting early haematoma expansion in ICH patients. | Jia-wei Zhong Yu-jia Jin Zai-jun Song Bo Lin Xiao-hui Lu Fang Chen Lu-sha Tong | 2021 | Stroke & Vascular Neurology2021，6，4： | 2 |
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