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| 1 | Effect of erhuangfang on cerebral and spinal demyelination and regeneration as well as expression of glial fibrillary acidic protein in rats with experimental allergic encephalomyelitis显示文摘BACKGROUND: It demonstrates that erhuangfang can improve clinical symptoms of multiple sclerosis and relieve side effects of hormone. However, whether erhuangfang can improve experimental allergic encephalomyelitis (EAE) or not needs a further study. OBJECTIVE: To observe the effect of erhuangfang on neuro-pathology and astrocyte in EAE rats and compare with the effect of hormone. DESIGN: Randomized controlled animal study. SETTINGS: Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University; College of Traditional Chinese Medicine, Capital Medical University. MATERIALS: The experiment was carried out in the Laboratory Center of Capital Medical University from August to October 2005. Ten adult guinea pigs (SPF grade, weighing 400-450 g) and 70 adult Lewis rats (SPF grade, weighing 200-220 g) were selected in this study. Erhuangfang consisted of jiudahuang, shengdi, shuizhi, dabeimu, etc. METHODS: ① Experimental intervention: Rats were randomly divided into normal group (n=10), model group (n=20), western medicine group (n=20) and Chinese herb group (n=20). Mixed emulsion, which was consisted of Freund's adjuvant and spinal cord homogenate of guinea pigs, was subcutaneously injected into palms of the two hindfeet of rats in the latter three groups to establish EAE models. Foot pads were injected with saline and then rats were perfused with saline in the normal group. In the model group, models were established as the same as those mentioned above, and rats were also perfused with saline. Rats in the western medicine group were perfused with saline and then 5 mg/kg prednisone acetate suspension. Rats in the Chinese herb group were perfused with erhuangfang decoction (15 g raw materials per kilogram) at 5 days before model establishment. The dosage in the four groups was 3 mL/day per rat. ② Experimental evaluation: At 28 days after model establishment, rats were randomly selected for cerebral (mainly surrounding cerebral ventricle) and spinal cord (cervical enlargement and lumbar enlargement) collections, and then haematine-eosin (HE) staining and SLG myelin staining were used to observe demyelination and regeneration; meanwhile, immunohistochemical staining was used to observe the expression of glial fibriliary acidic protein (GFAP). MAIN OUTCOME MEASURES: Cerebral and spinal demyelination and regeneration as well as expression of GFAP in EAE rats. RESULTS: All 70 Lewis rats were involved in the final analysis. ① Demyelination and regeneration: Infiltration of inflammatory cells surrounding cerebrum and small venous vessels of spinal cord white matter, demyelination surrounding vessels and plentiful foam cells at myelinolysis sites were observed in the model group. Symptoms were relieved in the western medicine group and the Chinese herb group as compared with those in the model group. While, numbers of inflammatory infiltrated cells and vascular cuffs were decreased in focal region as compared with those in the model group; in addition, areas of softening focus and demyelination were decreased. ② Expression of GFAP: Volumes and numbers of positive cells of GFAP in white matter region were respectively bigger and higher than those of normal cells in the model group. Plentiful positive cells of GFAP were disorderly aggregated in hippocampus and surrounding small vessel cuffs. While, expression of GFAP was mildly increased surrounding focus in the Chinese herb group; however, GFAP did not express surrounding focus in the western medicine group. In addition, expressions of GFAP were not increased in non-focal region in both Chinese herb group and western medicine group. CONCLUSION: Both erhuangfang and hormone can relieve inflammatory reaction of central nervoussystem and demyelination of EAE rats. On one hand, erhuangfang can regulate reaction of astrocyte in two ways, relieve reaction and proliferation of astrocyte in non-focal region and maintain the protective effect of astrocyte on brain tissue in focal region; on the other hand, hormone can overall inhibit reaction of astrocyte. | Xiuzhen Liu Yongping Fan Lei Wang Yun Cui Haiyang Gong | 2007 | Neural Regeneration Research2007，2，8： | 4 |
| 2 | Cooperative coverage ex- tension for relay-union networks 显示文摘 | Cui Yong Ma Xiao Cheng Xiuzhen | 2014 | Parallel and Distributed Sys- tems2014，26，2： | 1 |
| 3 | Achieving proportional fairness via AP power control in multi-rate WLANs显示文摘 | Li Wei Cui Yong Cheng Xiuzhen | 2011 | IEEE Transactions on Wireless Communications2011，10，11： | 1 |
| 4 | Na_(2)O/Li_(2)O Ratio Dependency on the Thermal,Mechanical,Dielectric Properties and Chemical Stabilities of Li_(2)O-Al_(2)O_(3)-SiO_(2) Glass显示文摘Li_(2)O-Al_(2)O_(3)-SiO_(2) based glasses were investigated as potential protection glass for electronic devices due to their excellent mechanical properties,such as high hardness,toughness,and scratch resistance.In this paper,Li_(2)O-Na_(2)O-Al_(2)O_(3)-SiO_(2) glass with different Li_(2)O/Na_(2)O ratio components were prepared by meltquenching method,and the effects of Na_(2)O/Li_(2)O ratio on the glass densities,structure,thermal,mechanical properties,and chemical stabilities were studied.The experimental results indicate that the glass transition temperature increases with the increases in Na_(2)O/Li_(2)O ratios,due to larger ion radius.While the thermal expansion coefficient slightly decreases from 11.4×10^(-6) to 11.09×10^(-6)/℃.The elastic modulus increases from 57 to 72 GPa.The bending strength reaches maximum 80.90 MPa when the Na_(2)O/Li_(2)O ratio is 1.7,then decreases as the ratio further increases.In addition,the Vicker’s hardness gets to 7.37 GPa with largest Na_(2)O/Li_(2)O ratio.Moreover,the dielectric loss and dielectric constant increases as the ratio increases.The Raman structure analysis shows the Q4[Si-O-Si]decreases as Na_(2)O/Li_(2)O ratio increases,which is responsible for the characteristic properties change.Moreover,the glass shows lowest mass loss in 10vol%HF solutions when the ratio is 1.4,while 1.7 in 5wt%NaOH solution. | 陈丽娜 谢俊 WANG Mingzhong CAO Shiyi CUI Xiuzhen LIANG Xinhui ZHANG Jihong HAN Jianjun HU Kaiwen | 2022 | Journal of Wuhan University of Technology(Materials Science)2022，37，6： | 0 |
| 5 | Decomposition Method of Genetic Correlation Coefficient Based on NC Ⅱ Mating Design显示文摘There are different degrees of correlation between crop traits. The phenotypic correlation is decomposed into genetic and environmental correlation in quantitative genetics. In this paper,according to stochastic model of variance and covariance analysis,we calculate different genetic components,bring up a decomposition method of genetic correlation coefficient based on NC II mating design,and use examples to show analytic steps and interpret results. | Wei WANG Zheng WANG Fengzhi WANG Songshan ZHAO Xiuzhen CUI | 2017 | Asian Agricultural Research2017，9，1： | 0 |
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