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| 1 | Marsdenia tenacissima extract induces G_0/G_1 cell cycle arrest in human esophageal carcinoma cells by inhibiting mitogen-activated protein kinase(MAPK) signaling pathway显示文摘Marsdenia tenacissima extract(MTE, trade name: Xiao-Ai-Ping injection) is an extract of a single Chinese plant medicine. It has been used for the treatment of cancer in China for decades, especially for esophageal cancer and other cancers in the digestive tract. In the present study, the potential mechanism for MTE's activity in esophageal cancer was explored. The effects of MTE on the proliferation of human esophageal cancer cells(KYSE150 and Eca-109) were investigated by the MTT assay, the Brd U(bromodeoxyuridine) incorporation immunofluorescence assay, and flow cytometric analysis. MTE inhibited cell proliferation through inducing G0/G1 cell cycle arrest in KYSE150 and Eca-109. Western blot analysis was employed to determine protein levels in the MTE treated cells. Compared with the control cells, the expression levels of the cell cycle regulatory proteins cyclin D1/D2/D3, cyclin E1, CDK2/4/6(CDK: cyclin dependent kinase), and p-Rb were decreased significantly in the cells treated with MTE at 40 mg·m L-1. In addition, MTE had an inhibitory effect on the MAPK(mitogen-activated protein kinase) signal transduction pathway, including ERK(extracellular signal-regulated kinase), JNK(c-Jun N-terminal kinase), and p38 MAPK. Moreover, MTE showed little additional effects on the regulation of cyclin D1/D3, CDK4/6, and p-Rb when the ERK pathway was already inhibited by the specific ERK inhibitor U0126. In conclusion, these data suggest that MTE inhibits human esophageal cancer cell proliferation through regulation of cell cycle regulatory proteins and the MAPK signaling pathways, which is probably mediated by the inhibition of ERK activation. | FAN Wei SUN Li ZHOU Jing-Qian ZHANG Cang QIN Song TANG Ying LIU Yang LIN Sen-Sen YUAN Sheng-Tao | 2015 | Chinese Journal of Natural Medicines2015，13，6： | 32 |
| 2 | Complementation in the composition of steel slag and red mud for preparation of novel ceramics显示文摘A method for preparing novel ceramics was developed in this study. Different ratios red muds were added to steel slags to optimize the preparation of novel ceramics by a traditional ceramic preparation process. The sintering mechanism, microstructure, and performance were studied by X-ray diffraction techniques, scanning electron microscopy, and combined experiments of linear shrinkage, water absorption, and flexural strength. The results confirmed that red mud can reduce the volumetric instabilities through the complementarity of red mud and ferroalloy slag. The crystal phases in the ceramics are all pyroxene group minerals, including diopside ferrian, augite, and diopside. The flexural strength of the ceramic that contains 40 wt% red mud and was prepared at the optimal sintering temperature(1140°C) is greater than 93 MPa; its corresponding water absorption is less than 0.05%. | Yan-bing Zong Wen-hui Chen Yong Fan Tai-lin Yang Zhao-bo Liu Da-qiang Cang | 2018 | International Journal of Minerals,Metallurgy and Materials2018，25，9： | 7 |
| 3 | Effects of sintering atmosphere on the physical and mechanical properties of modified BOF slag glass显示文摘This study proposes an efficient way to utilize all the chemical components of the basic oxygen furnace(BOF) slag to prepare high value-added glass-ceramics.A molten modified BOF slag was converted from the melting BOF slag by reducing it and separating out iron component in it,and the modified BOF slag was then quenched in water to form glasses with different basicities.The glasses were subsequently sintered in the temperature range of 600–1000°C in air or nitrogen atmosphere for 1 h.The effects of different atmospheres on the physical and mechanical properties of sintered samples were studied by using differential scanning calorimetry(DSC),X-ray diffraction(XRD) and scanning electron microscopy(SEM) and by conducting experiment on evaluating the sintering shrinkage,water absorption and bulk density.It is found that the kinetics of the sintering process is significantly affected by sintering atmosphere.In particular,compared with sintering in air atmosphere,sintering in N2 atmosphere promotes the synergistic growth of pyroxene and melilite crystalline phases,which can contribute to better mechanical properties and denser microstructure. | Wen-bin Dai Yu Li Da-qiang Cang Yuan-yuan Zhou Yong Fan | 2014 | International Journal of Minerals,Metallurgy and Materials2014，21，5： | 2 |
| 4 | Transformation of polychlorinated biphenyls by persulfate at ambient temperature显示文摘 | Fang GD Dionysiou DD Zhou DM Wang Y Zhu XD Fan JX Cang L Wang YJ | 2013 | Chemosphere2013，90，： | 1 |
| 5 | Shear sliding-off fracture of bulk amorphous Zr-based alloys containing nanoscale compound particles 显示文摘 | Cang Fan Inoue A | 1999 | Mater Trans JIM1999，40，12： | 1 |
| 6 | Ductility of bulk nanocrystalline composite and metallic glasses at room temperature显示文摘 | Cang Fan Inoue A | 2000 | Appl Phys Lett2000，77，1： | 1 |
| 7 | Nitrous Oxide‐Oxygen Mixture During Burn Wound Dressing: A Double‐Blind Randomized Controlled Study显示文摘 | Yu‐Xiang Li Wen‐Jun Han Hong‐Tai Tang Yin‐Sheng Wu Lu Tang Jian‐Qiang Yu Yan‐Cang Li Zhao‐Fan Xia Ji‐Jun Zhao | 2013 | CNS Neurosci Ther2013，，： | 1 |
| 8 | High Strength Bulk Nanocrystalline Alloys in a Zr-based System Containing Compound and Glass Phases 显示文摘 | Inoue A Fan Cang Takeuchi A | 1999 | Non-cryst Solids1999，，： | 1 |
| 9 | Surfactants-enhanced electrokinetic transport of xanthan gum stabilized nanoPd/Fe for the remediation of PCBs contaminated soils显示文摘 | Guangping Fan Long Cang Wenxiu Qin Chuifan Zhou Helena I. Gomes Dongmei Zhou | 2013 | Separation and Purification Technology2013，，： | 1 |
| 10 | Thermal fatigue behavior of Al-Si/SiCp composite synthesized by spray deposition显示文摘 | LI Wei CHEN Zhen-hua CHEN Ding FAN Cang WANG Can-rang | 2010 | Journal of Alloys and Compounds2010，504，： | 1 |
| 11 | Shear sliding-off fracture of bulk amorphous Zr-based alloys containing nanoscale com?pound particles显示文摘 | Cang Fan Inoue A | 1999 | Mater TransJIM1999，40，12： | 1 |
| 12 | Enhanced-electrokinetic remediation of copper-pyrene co-contaminated soil with different oxidants and p H control显示文摘 | Cang L Fan G Zhou D | 2013 | Chemosphere2013，90，8： | 1 |
| 13 | Transformation of polychlorinated biphenyls by persulfate at ambient temperature显示文摘 | Guo-Dong Fang Dionysios D. Dionysiou Dong-Mei Zhou Yu Wang Xiang-Dong Zhu Jian-Xin Fan Long Cang Yu-Jun Wang | 2012 | Chemosphere2012，，： | 1 |
| 14 | Thermal fatigue behavior of Al–Si/SiC p composite synthesized by spray deposition显示文摘 | Li Wei Chen Zhenhua Chen Ding Fan Cang Wang Canrang | 2010 | Journal of Alloys and Compounds2010，，： | 1 |
| 15 | Enhanced-electrokinetic remediation of copper–pyrene co-contaminated soil with different oxidants and pH control显示文摘 | Long Cang Guang-Ping Fan Dong-Mei Zhou Quan-Ying Wang | 2012 | Chemosphere2012，，： | 1 |
| 16 | High-strength Bulk Nanocrystalline Alloys in a Zr-Based System Containing Compound and Glassy Phases 显示文摘 | Inoue A FAN Cang Takenchi A | 1999 | Journal of Non-crystalline Solids1999，，： | 1 |
| 17 | 极长链酰基辅酶A脱氢酶缺乏症ACADVL基因新发突变导致线粒体功能障碍显示文摘目的:鉴定并阐述6个ACADVL新发突变的对细胞和分子功能的影响,为后期分子诊断和临床病例管理提供科学依据。创新点:本文从分子功能和结构的角度阐述6个新位点对蛋白和细胞功能的不同影响。方法:通过体外构建突变载体建立各突变的过表达细胞系,检测各细胞系脂肪酸代谢能力、线粒体呼吸链功能、线粒体产三磷酸腺苷(ATP)能力、活性氧(ROS)和凋亡水平。同时检测了新发突变对蛋白二聚体稳定性的影响并用分子动力学模拟了突变蛋白构像的改变。结论:突变(c.541C>T、c.863T>G、c.895A>G、c.1238T>C、c.1276G>A和c.1505T>A)过表达细胞系均表现出脂肪酸代谢障碍,线粒体呼吸链功能障碍,ATP产生水平下降和线粒体ROS水平上升。在无糖条件下,各细胞系的凋亡水平上升。各突变影响了极长链酰基辅酶A脱氢酶(VLCAD)蛋白二聚体的稳定性,分子动力学模拟预测每个突变均会导致VLCAD蛋白结构的发生重要构象变化。 | Ting CHEN Fan TONG Xiao-yu WU Ling ZHU Qiu-zi YI Jing ZHENG Ru-lai YANG Zheng-yan ZHAO Xiao-hui CANG Qiang SHU Ping-ping JlANG | 2020 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2020，21，11： | 1 |
| 18 | High-strength ulk nanocrystalline alloys in a Zr-based system containing conpound and glass phases显示文摘 | Inoue A Fan Cang Takeuchi A | 1999 | Noncryst solids1999，，： | 1 |
| 19 | Surfac- rants-enhanced electrokinetic transport of xanthan gum stabi- lized nano Pd/Fe for the remediation of PCBs contaminated soils显示文摘 | FAN Guangping CANG Long QIN Wenxiu | 2013 | Separation and Purification Technology2013，114，13： | 1 |
| 20 | Dry powder inhalations containing thymopentin and its immunomodulating effects in Wistar rats显示文摘 | Jing Gao Xueying Ding Cang Chu Lei Lu Yi Zhang Yan Chen Wei Fan Guodong Li Shen Gao | 2008 | European Journal of Pharmaceutical Sciences2008，，： | 1 |
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