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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Genetic and pharmacological inhibition of the 5-lipoxygenase/leukotriene pathway in atherosclerotic lesion development in ApoE deficient mice显示文摘 | Cao RY St AT Grabner R | 2009 | Atherosclerosis2009，203，2： | 1 |
| 3 | An overview of recent progress in the study of distributed multi-agent coordination 显示文摘 | Cao Y C Yu W W Ren W et at | 2013 | IEEE Transactions on Industrial Informatics2013，9，42： | 1 |
| 4 | The property research on high - entropy alloy AlxFeCoNiCuCr Coating by laser cladding显示文摘 | Le X Y Ma M X Cao Y X et at | 2011 | Physics Procedia2011，12，： | 1 |
| 5 | RANTES gene polymorphisms and risk of pediatric asthma: a meta-analysis显示文摘 | LU Y M CAO L F LI Y Q et at | 2012 | Exp Ther Med2012，4，5： | 1 |
| 6 | Intracellular unesterified arachidonic acid signals apoptosis 显示文摘 | Cao Y Pearman AT Zimmerman GA | 2000 | Proc Nail Acad Sci U S A2000，97，11： | 1 |
| 7 | Impact of intense pulse light irradiation on BALB/c mouse skin-in vivo study on collagens,matrix metalloproteinases and vascular endothelial growth factor显示文摘 | Luo D Cao Y Wu D et at | 2009 | Lasers Med Sci2009，24，1： | 1 |
| 8 | Intracellular unestedfied arachidonic acid sigmals apoptosis显示文摘 | Pearman AT Zimmerman GA | 2000 | Proc Ntul Aead Sci USA2000，97，11： | 1 |
| 9 | Intracellular unesterified arachidonic acid signals apoptosis显示文摘 | Cao Y Pearman AT Zimmerman GA | | 0，，： | 1 |
| 10 | Intracellularunesterified arachidonic acid signals apoptosis显示文摘 | Cao Y Pearman AT Zimmerman GA | 2000 | Proc Natl Acad Sci USA2000，97，11： | 1 |
| 11 | Preparation of Au-BiVO4 heterogeneous nanostructures as highly efficient visible-light photocatalysts显示文摘 | Cao S W Yin Z Barber J et at | 2012 | ACS Appl Mater Interf2012，4，： | 1 |
| 12 | Thl7 cells upregulate poly- meric Ig receptor and intestinal IgA and contribute to intestinal homeostasis 显示文摘 | Cao AT Yao S Gong B | 2012 | Journal of immunology2012，189，： | 1 |
| 13 | Isotope geochemistry of the Shihu gold deposit, Hebei Province, North China: Implica- tion for the source of ore fluid and materials显示文摘 | Cao Y Li S R Yao M J et at | 2011 | Carpathian Journal of Earth and Environmental Sciences2011，6，2： | 1 |
| 14 | Local Application of Morphine Suppresses Glutamate-evoked Activities of C and Aδ Afferent Fibers in Rat Hairy Skin显示文摘 | Tian Y t Guo Y Cao D Y et at | 2005 | Brain Research2005，1059，1： | 1 |
| 15 | Diversity of viruses associated with acute gastroenteritis in children hospitalized with diarrhea in Ho Chi Minh City, Vietnam 显示文摘 | Nguyen TA Yagyu F Okame M Phan TG Trinh QD Yan H Hoang KT Cao AT Le Hoang P Okitsu S Ushijima H | 2007 | J Med Virol2007，79，5： | 1 |
| 16 | Integrated analysis of long noncoding RNA and coding RNA expression in esophageal squamous cell carci- noma显示文摘 | Cao W Wu W Shi F et at | 2013 | Int J Genomics2013，2013，48： | 1 |
| 17 | Proxy re-encryption with key- word search显示文摘 | SHAO J CAO Z LIANG X H et at' | 2010 | Information Sciences2010，180，4： | 1 |
| 18 | A novel secreted protein toxin from the insect pathogenic bacterium Xenorhabdus nematophila显示文摘 | Brown SE Cao AT Hines ER Akhurst RJ East PD | | 0，，15： | 1 |
| 19 | Electrochemical properties of anatase TiO2 nanotubes as an anode material for lithium-ion batteries显示文摘 | Xu J W Ha C H Cao B et at | 2007 | ElectrochimicaActa2007，52，28： | 1 |
| 20 | Intracellular unesterified arachidonic acid signals apoptosis显示文摘 | Cao Y Pearman AT Zimmerman GA | 2000 | Proc Natl Acad Sci USA2000，97，11： | 1 |
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