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| 1 | Beclin-1/LC3-II dependent macroautophagy was uninfluenced in ischemia-challenged vascular endothelial cells显示文摘Autophagy has been extensively studied and occurs in many biological settings.However,a question remains as to whether ischemia enhances Beclin-1/LC3-II-dependent macroautophagy in vascular endothelial cells,as has been previously thought.Furthermore,the effect of the level of autophagy on cell or skin flap survival still requires elucidation.We created a lethal ischemia model in human umbilical vascular endothelial cells(HUVECs),performed quantitative proteomics and bioinformatics analyses,and verified the autophagic status and effect both in vitro and in vivo.The significantly upregulated proteins encoded by autophagy-related genes(ATGs)included ATG2A,ATG3,ATG4B,ATG5,ATG7,ATG9A,ATG12,ATG16,and ATG101.The significantly enhanced lysosomal proteins were cathepsin B,cathepsin D,lysosome-associated membrane protein 1(LAMP1),and LAMP2.However,the differentially expressed proteins excluded Beclin-1,microtubule-associated protein light chain 3(LC3)-I,and LC3-II.Western blot analyses verified that the protein expression levels of Beclin-1,LC3-I,and LC3-II were neither upregulated nor downregulated in ischemia-challenged HUVECs.The autophagic status was not enhanced by rapamycin in ischemic HUVECs but appeared to be inhibited by chloroquine.Our in vivo study on rats showed that a downregulation in autophagic status jeopardized skin flap survival.In conclusion,Ischemia neither enhanced nor inhibited Beclin-1/LC3-II-dependent canonical macroautophagy both in vitro and in vivo,in contradiction to previous studies.An appropriate autophagic homeostasis can minimize cell or skin flap damage. | Yaping Ma Chaofan Li Yan He Tiwei Fu Li Song Qingsong Ye Fugui Zhang | 2022 | Genes & Diseases2022，9，2： | 3 |
| 2 | Transcriptome-Wide Identification and Validation of Reference Genes in Black Rockfish(Sebastes schlegelii)显示文摘The quantitative real-time reverse transcription PCR(qRT-PCR)is a widely used technique to analyze gene expression levels.Selecting a suitable reference gene is a crucial step to obtain an accurate result in qRT-PCR.However,most previous studies on fishes adopted reference genes that were commonly used in mammals without validation.In this study,we utilized 89 transcrip-tome datasets covering early developmental stages and different adult tissues,and carried out transcriptome-wide identification and validation of reference genes in Sebastes schlegelii.Finally,121 candidate reference genes were identified based on four criteria.Eight candidates(METAP2,BTF3L4,EIF5A1,TCTP,UBC,PAIRB,RAB10,and DLD)and four commonly used reference genes in mam-mals(TUBA,ACTB,GAPDH,RPL17)were selected for validation via qRT-PCR and four statistical analysis methods(delta-Ct,Best-Keeper,geNorm,and NormFinder).The results indicated that when the black rockfish are cultured in a general condition,the eight candidate reference genes are more stable than traditional reference genes in mammals,and RAB10,EIF5A1,PAIRB and BTF3L4 are the best reference genes in rockfish.This is the first study to conduct transcriptome-wide identification and validation of reference genes for quantitative RT-PCR in the black rockfish,and lay an important foundation for gene expression analysis in teleost. | JIN Chaofan SONG Weihao WANG Mengya QI Jie ZHANG Quanqi HE Yan | 2021 | Journal of Ocean University of China2021，20，3： | 1 |
| 3 | 边缘计算能否结束数据流的带宽之争?显示文摘带宽之争通常起源于数据拥堵的四个常见原因:多个设备之间的数据差异、数据阻塞现象、缺少隔离引起的数据安全性问题以及可靠性不高。本文将研究边缘计算如何解决这些问题并结束带宽之争。随着互联设备、装置以及家电设备数量的激增,网络中充斥着越来越多的不兼容数据。比如在一栋房子里面,除了平板电脑、个人电脑(PC)、智能手机和汽车之外,还有媒体中心和音响系统、气候控制系统、家用电器、健康监视器、互动设备(如Alexa)等消费类互联设备,所有这些都将网络压力推向了极限。 | Bin Jiang Chaofan Ma Huifang Xu Houbing Song | 2019 | 中国集成电路2019，28，7： | 0 |
| 4 | Application and development of ground source heat pump technology in China显示文摘Ground source heat pumps (GSHPs) are one of the renewable energy technologies with features of high efficiency, energy saving, economic feasibility and environmental protection. In China, GSHPs have been widely used for building heating and cooling in recent years, and have shown great potential for future energy development. This paper summarizes the classification, development history, and use status of shallow GSHPs. Several typical engineering cases of GSHP technology are also specified and analyzed. Finally, promising development trends and some advanced technologies are illustrated. | Chaofan Song Yang Li Taha Rajeh Ling Ma Jun Zhao Wenjia Li | 2021 | Protection and Control of Modern Power Systems2021，6，1： | 0 |
| 5 | Cache-Enabled in Cooperative Cognitive Radio Networks for Transmission Performance显示文摘The proliferation of mobile devices that support the acceleration of data services(especially smartphones)has resulted in a dramatic increase in mobile traffic. Mobile data also increased exponentially, already exceeding the throughput of the backhaul. To improve spectrum utilization and increase mobile network traffic, in combination with content caching, we study the cooperation between primary and secondary networks via content caching. We consider that the secondary base station assists the primary user by pre-caching some popular primary contents.Thus, the secondary base station can obtain more licensed bandwidth to serve its own user. We mainly focus on the time delay from the backhaul link to the secondary base station. First, in terms of the content caching and the transmission strategies, we provide a cooperation scheme to maximize the secondary user’s effective data transmission rates under the constraint of the primary users target rate. Then, we investigate the impact of the caching allocation and prove that the formulated problem is a concave problem with regard to the caching capacity allocation for any given power allocation. Furthermore, we obtain the joint caching and power allocation by an effective bisection search algorithm. Finally, our results show that the content caching cooperation scheme can achieve significant performance gain for the primary and secondary systems over the traditional two-hop relay cooperation without caching. | Jiachen Yang Chaofan Ma Jiabao Man Huifang Xu Gan Zheng Houbing Song | 2020 | Tsinghua Science and Technology2020，25，1： | 0 |
| 6 | Brain Functional Networks with Dynamic Hypergraph Manifold Regularization for Classification of End-Stage Renal Disease Associated with Mild Cognitive Impairment显示文摘The structure and function of brain networks have been altered in patients with end-stage renal disease(ESRD).Manifold regularization(MR)only considers the pairing relationship between two brain regions and cannot represent functional interactions or higher-order relationships between multiple brain regions.To solve this issue,we developed a method to construct a dynamic brain functional network(DBFN)based on dynamic hypergraph MR(DHMR)and applied it to the classification of ESRD associated with mild cognitive impairment(ESRDaMCI).The construction of DBFN with Pearson’s correlation(PC)was transformed into an optimization model.Node convolution and hyperedge convolution superposition were adopted to dynamically modify the hypergraph structure,and then got the dynamic hypergraph to form the manifold regular terms of the dynamic hypergraph.The DHMR and L_(1) norm regularization were introduced into the PC-based optimization model to obtain the final DHMR-based DBFN(DDBFN).Experiment results demonstrated the validity of the DDBFN method by comparing the classification results with several related brain functional network construction methods.Our work not only improves better classification performance but also reveals the discriminative regions of ESRDaMCI,providing a reference for clinical research and auxiliary diagnosis of concomitant cognitive impairments. | Zhengtao Xi Chaofan Song Jiahui Zheng Haifeng Shi Zhuqing Jiao | 2023 | Computer Modeling in Engineering & Sciences2023，，6： | 0 |
| 7 | Tunable quantum dots in monolithic Fabry-Perot microcavities for high-performance single-photon sources显示文摘Cavity-enhanced single quantum dots(QDs)are the main approach towards ultra-high-performance solid-state quantum light sources for scalable photonic quantum technologies.Nevertheless,harnessing the Purcell effect requires precise spectral and spatial alignment of the QDs’emission with the cavity mode,which is challenging for most cavities.Here we have successfully integrated miniaturized Fabry-Perot microcavities with a piezoelectric actuator,and demonstrated a bright single-photon source derived from a deterministically coupled QD within this microcavity.Leveraging the cavity-membrane structures,we have achieved large spectral tunability via strain tuning.On resonance,a high Purcell factor of~9 is attained.The source delivers single photons with simultaneous high extraction efficiency of 0.58,high purity of 0.956(2)and high indistinguishability of 0.922(4).Together with its compact footprint,our scheme facilitates the scalable integration of indistinguishable quantum light sources on-chip,therefore removing a major barrier to the development of solid-state quantum information platforms based on QDs. | Jiawei Yang Yan Chen Zhixuan Rao Ziyang Zheng Changkun Song Yujie Chen Kaili Xiong Pingxing Chen Chaofan Zhang Wei Wu Ying Yu Siyuan Yu | 2024 | Light(Science & Applications)2024，13，2： | 0 |
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