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| 1 | C_(17)-fengycin B,produced by deep-sea-derived Bacillus subtilis,possessing a strong antifungal activity against Fusarium solani显示文摘Root rot disease caused by Fusarium solani is the most devastating disease of the tomato and legume crops in China.The metabolites of Bacillus species can inhibit many fungal diseases.In this study,the metabolites of deep-sea-derived bacterium Bacillus subtilis 2 H11 can significantly inhibit the growth of F.solani.The metabolite C_(17)-fengycin B,one of the cyclic lipopeptides,was identified by the combination of silica column chromatography,high-performance liquid chromatography(HPLC),high-energy collision induced dissociation mass spectrometry(HCD-MS)and tandem mass spectrometry(HCD-MS/MS).The results of scanning electron microscopy(SEM)and transmission electron microscopy(TEM)showed that C_(17)-fengycin B could destroy the structure of the hyphae and spores of F.solani.The antifungal activities of C_(17)-fengycin B against F.solani were tested at concentrations ranging from 0.05 mg/mL to 0.20 mg/mL.The results indicated that C_(17)-fengycin B inhibited the growth of F.solani with antifungal index of 89.80%at 0.20 mg/mL,and the antifungal activity of C_(17)-fengycin B was further verified by the pot experiment.In addition,the cytotoxicity experiment showed that C_(17)-fengycin B had good biocompatibility and was a potential candidate for the development of biocontrol pesticide in the future. | Weixiang LIU Chaomin SUN | 2021 | Journal of Oceanology and Limnology2021，39，5： | 5 |
| 2 | Observation and research of deep underground multi-physical fields—Huainan–848 m deep experiment显示文摘Compared with the surface,the deep environment has the advantages of allowing“super-quiet and ultra-clean”-geophysical field observation with low vibration noise and little electromagnetic interference,which are conducive to the realization of long-term and high-precision observation of multi-physical fields,thus enabling the solution of a series of geoscience problems.In the Panyidong Coal Mine,where there are extensive underground tunnels at the depth of 848 m below sea level,we carried out the first deep-underground geophysical observations,including radioactivity,gravity,magnetic,magnetotelluric,background vibration and six-component seismic observations.We concluded from these measurements that(1)the background of deep subsurface gravity noise in the long-period frequency band less than 2 Hz is nearly two orders of magnitude weaker than that in the surface observation environment;(2)the underground electric field is obviously weaker than the surface electric field,and the relatively high frequency of the underground field,greater than 1 Hz,is more than two orders of magnitude weaker than that of the surface electric field;the east-west magnetic field underground is approximately the same as that at the surface;the relatively high-frequency north-south magnetic field underground,below 10 Hz,is at least one order of magnitude lower than that at the surface,showing that the underground has a clean electromagnetic environment;(3)in addition to the highfrequency and single-frequency noises introduced by underground human activities,the deep underground space has a significantly lower background vibration noise than the surface,which is very beneficial to the detection of weak earthquake and gravity signals;and(4)the underground roadway support system built with ferromagnetic material interferes the geomagnetic field.We also found that for deep observation in the“ultra-quiet and ultra-clean”environment,the existing geophysical equipment and observation technology have problems of poor adaptability and insufficient precision as well as data cleaning problems,such as the effective separation of the signal and noise of deep observation data.It is also urgent to interpret and comprehensively utilize these high-precision multi-physics observation data. | Yun WANG Yaxin YANG Heping SUN Chengliang XIE Qisheng ZHANG Xiaoming CUI Chang CHEN Yongsheng HE Qiangqiang MIAO Chaomin MU Lianghui GUO Jiwen TENG | 2023 | Science China Earth Sciences2023，66，1： | 3 |
| 3 | COVID-19-associated gastrointestinal and liver injury:clinical features and potential mechanisms显示文摘Coronavirus disease-2019(COVID-19)is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The infection is spreading globally and poses a huge threat to human health.Besides common respiratory symptoms,some patients with COVID-19 experience gastrointestinal symptoms,such as diarrhea,nausea,vomiting,and loss of appetite.SARS-CoV-2 might infect the gastrointestinal tract through its viral receptor angiotensin-converting enzyme 2(ACE2)and there is increasing evidence of a possible fecal–oral transmission route.In addition,there exist multiple abnormalities in liver enzymes.COVID-19-related liver injury may be due to drug-induced liver injury,systemic inflammatory reaction,and hypoxia–ischemia reperfusion injury.The direct toxic attack of SARS-CoV-2 on the liver is still questionable.This review highlights the manifestations and potential mechanisms of gastrointestinal and hepatic injuries in COVID-19 to raise awareness of digestive system injury in COVID-19. | Peijie Zhong Jing Xu Dong Yang Yue Shen Lu Wang Yun Feng Chunling Du Yuanlin Song Chaomin Wu Xianglin Hu Yangbai Sun | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 1 |
| 4 | Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination显示文摘 | Huadong Pei Jingfang Liu Yun Cheng Chaomin Sun Chen Wang Yueping Lu Jie Ding Jian Zhou Hua Xiang | 2005 | Applied Microbiology and Biotechnology2005，，2： | 1 |
| 5 | Complete sequence and molecular characterization of pNB101, a rolling-circle replicating plasmid from the haloalkaliphilic archaeon Natronobacterium sp. strain AS7091显示文摘 | Meixian Zhou Hua Xiang Chaomin Sun Yun Li Jingfang Liu Huarong Tan | 2004 | Extremophiles2004，，2： | 1 |
| 6 | A deep‐sea sulfate‐reducing bacterium generates zero‐valent sulfur via metabolizing thiosulfate显示文摘Zero‐valent sulfur(ZVS)is a crucial intermediate in the sulfur geobiochemical circulation and is widespread in deep‐sea cold seeps.Sulfur‐oxidizing bacteria are thought to be the major contributors to the formation of ZVS.However,ZVS production mediated by sulfate‐reducing bacteria(SRB)has rarely been reported.In this study,we isolated and cultured a typical SRB designated Oceanidesulfovibrio marinus CS1 from deep‐sea cold seep sediment in the South China Sea.We show that O.marinus CS1 forms ZVS in the medium supplemented with thiosulfate.Proteomic and protein activity assays revealed that thiosulfate reductase(PhsA)and the sulfide:quinone oxidoreductase(SQR)played key roles in driving ZVS formation in O.marinus CS1.During this process,thiosulfate firstly was reduced by PhsA to form sulfide,then sulfide was oxidized by SQR to produce ZVS.The expressions of PhsA and SQR were significantly upregulated when O.marinus CS1 was cultured in a deep‐sea cold seep,strongly indicating that strain CS1 might form ZVS in the deep‐sea environment.Notably,homologs of phsA and sqr were widely identified from microbes living in sediments of deep‐sea cold seep in the South China Sea by the metagenomic analysis.We thus propose that SRB containing phsA and sqr genes potentially contribute to the formation of ZVS in deep‐sea cold seep environments. | Rui Liu Yeqi Shan Shichuan Xi Xin Zhang Chaomin Sun | 2022 | mLife2022，1，3： | 0 |
| 7 | Screening and characterization of proteases produced by deep-sea cold seep bacteria显示文摘Fifty protease-producing strains were screened from sediment of deep-sea cold seep,and divided into four diff erent categories:Bacillus,Pseudoalteromonas,Vibrio,and Alteromonas according to the sequences of 16s rRNA.Their abilities to produce protease,amylase,and lipase were determined,and a Bacillus strain gcc-1 displayed very strong alkaline protease activity and stability under different thermal and acidic conditions.The purifi cation of the protease produced by strain gcc-1 was carried out by precipitation with ammonium sulfate,and sequentially chromatographed by anion exchange column and gel filtration.The purifi ed protease showed a single band at the molecular weight of 28 kDa by SDS-PAGE.The characterization results show that the purified protease exhibited a considerable activity and stability in a wide thermal range of 10-80℃and a wide acidic range of pH 6.5-11.5,and displayed highest activity at 40℃ and pH 8.5.Notably,the protease still maintained high activity even at low to 10℃.Furthermore,the protease exhibited good stability in presence of diff erent surfactants,organic solvents,oxidizing agent H_(2)O_(2),and commercial detergents.Therefore,the protease produced by gcc-1 is a cold active and high stable enzyme,and has a promising potential in laundry detergent as an additive. | Chenchen GUO Chaomin SUN Shimei WU | 2022 | Journal of Oceanology and Limnology2022，40，2： | 0 |
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