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| 1 | The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding显示文摘Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs. | Xiaoya Shi Shuo Cao Xu Wang Siyang Huang Yue Wang Zhongjie Liu Wenwen Liu Xiangpeng Leng Yanling Peng Nan Wang Yiwen Wang Zhiyao Ma Xiaodong Xu Fan Zhang Hui Xue Haixia Zhong Yi Wang Kekun Zhang Amandine Velt Komlan Avia Daniela Holtgräwe Jérôme Grimplet JoséTomás Matus Doreen Ware Xinyu Wu Haibo Wang Chonghuai Liu Yuling Fang Camille Rustenholz Zongming Cheng Hua Xiao Yongfeng Zhou | 2023 | Horticulture Research2023，10，5： | 3 |
| 2 | Genetic transformation of wheat mediated by Agrobacterium tumefaciens显示文摘 | Cheng M Fry J E Pang S Zhou H Hironaka C M Duncan D R Conner T W Wan Y | 1997 | Plant Physiol1997，，： | 2 |
| 3 | On-line flow displacement immunoassay for fatty acid-binding protein显示文摘 | Kaptein WA Korf J Cheng S | | 0，，： | 2 |
| 4 | Effect of saikosaponin, a triterpene saponin, on apoptosis in lymphocytes: association with c-myc, p53, and bc1-2 mRNA 显示文摘 | Hsu M J Cheng J S Huang H C | 2000 | Br J Pharmacol2000，131，7： | 2 |
| 5 | Effects of the phase-separated melt on crystallization behavior and morphology in short chain branched metallocene polyethylenes显示文摘 | Fu Q Chiu F C McCreight K W Guo M Tseng W W Cheng S Z Keating M Y Hsieh E T and DesLauriers P J | | 0，，： | 2 |
| 6 | Incoherent Coincidence Imaging and Its Applicability in X-ray Diffraction 显示文摘 | CHENG J HANS S | 2004 | Phys Rev Lett2004，92，09： | 1 |
| 7 | A study on technique to estimate impact location of loose part using Wigner-Ville distribution显示文摘 | KIM Y B KIM S J CHENG H D | 2003 | Progress in Nuclear Energy2003，43，： | 1 |
| 8 | A new artificial neural network based response surface method for structural reliability analysis显示文摘 | Cheng J Li Q S Xiao R | 2008 | Probabilistic Engineering Mechanics2008，23，1： | 1 |
| 9 | The leukemia-associated cytoplasmic nucleophosmin mutant is an oncogene with paradoxical functions : Arf inactivation and induction of cellular senescence 显示文摘 | Cheng K Grisendi S Clohessy J G | 2007 | Oncogene2007，26，53： | 1 |
| 10 | Tissue factor-a receptor involved in the control of cellular properties,including angiogenesis显示文摘 | Cheng J Bierhaus A Schiekofer S | 2001 | Thromb Haemost2001，86，1： | 1 |
| 11 | Efficacy and safety of sorafenib in patients in the Asia Pacific region with advanced hepatocellular carcinoma: a phase Ⅲ randomised,double-blind, placebo controlled trial显示文摘 | Cheng A L Kang Y K Chen Z Tsao C J Qin S Kim J S | 2009 | Lancet Oncol2009，10，： | 1 |
| 12 | Functions and roles of the-extracellular Ca^2 + -sensing receptor in the gastrointestinal tract显示文摘 | Hebert SC Cheng S Geibel J | 2004 | Cell Calcium2004，35，3： | 1 |
| 13 | SUMO-Specific Protease 1 Is Essential for Stabilization of HIFla during Hypoxia 显示文摘 | Cheng J Kang X Zhang S | 2007 | Cell2007，131，3： | 1 |
| 14 | Modulation of C-reactive protein-mediated monocyte chemoattractant protein-1 induction in human endothelial cells by anti-atherosclerosis drugs显示文摘 | PASCERI V CHENG J S WILLERSON J T | 2001 | Circulation2001，103，21： | 1 |
| 15 | The application of competitive hopfield neural network to medical image segmentation显示文摘 | CHENG K S LIN J S MAO C W | 1996 | IEEE T Med Imaging1996，15，4： | 1 |
| 16 | Hyperhomocysteinaemia and premature coroaary artery disease in the Chinese显示文摘 | Lolin Y Sanderson J E Cheng S K | 1996 | Heart1996，76，： | 1 |
| 17 | Effects of metallic cobalt addition on the performance of pasted nickel electrodes 显示文摘 | YUAN A B CHENG S A ZHANG J Q | 1999 | J Power Sources1999，77，： | 1 |
| 18 | Relative contribution of bacteria,protozoa,and fungi to in vitro degradation of orchard grass cell wall and their interactions显示文摘 | Lee S S Ha J K Cheng K J | 2000 | Applied and Environmental Microbiology2000，66，9： | 1 |
| 19 | Sizing resin structure and interphase formation in carbon fiber composites 显示文摘 | Cheng T H Zhang J Yumitori S | 1994 | Composites1994，25，7： | 1 |
| 20 | Frost formation on a super-hydrophobic surface under natural convection conditions 显示文摘 | Liu Z L Gou Y J WangJ T Cheng S Y | 2008 | International Journal of Heat and Mass Transfer2008，51，25： | 1 |
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