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| 1 | Functional difference between Sinorhizobium meliloti NifA and Enterobacter cloacae NifA显示文摘The nifA gene is an important regulatory gene and its product, NifA protein, regulates the expression of many nif genes involved in the nitrogen fixation process. We introduced multiple copies of the constitutively expressed Sinorhizobium meliloti (Sm) or Enterobacter cloacae (Ec) nifA gene into both the nifA mutant strain SmY and the wild-type strain Sm1021. Root nodules produced by SmY containing a constitutively expressed Sm nifA gene were capable of fixing nitrogen, while nodules produced by SmY containing the Ec nifA gene remained unable to fix nitrogen, as is the case for SmY itself. However, transfer of an additional Sm nifA gene into Sm1021 improved the nitrogen-fixing efficiency of root nodules to a greater extent than that observed upon transfer of the Ec nifA gene into Sm1021. Comparative analysis of amino acid sequences between Sm NifA and Ec NifA showed that the N-terminal domain was the least similar, but this domain is indispensable for complementation of the Fix? phenotype of SmY by Sm NifA. We conclude that more than one domain is involved in determining functional differences between Sm NifA and Ec NifA. | YANG Chengtao YU Guanqiao SHEN Shanjiong(San Chiun Shen) ZHU Jiabi | 2004 | Science China(Life Sciences)2004，47，1： | 2 |
| 2 | An evaluation method of contact measurement system of the coordinate measuring machine 显示文摘 | Yu Chengtao Ma Chih-Min Cheng Bor-Wen | 2009 | Journal of Quality2009，16，3： | 1 |
| 3 | Giant magnetoresistance in Fe/Ag multilayers and its anomalous temperature dependence显示文摘 | YU Chengtao LI Shuxiang LAI Wuyan | 1995 | Physical Review B1995，52，： | 1 |
| 4 | Influence of processing medium on frictional wear properties of ball bearing steel prepared by laser surface melting coupled with bionic principles显示文摘 | Hong Zhou Chengtao Wang Qingchun Guo Jiaxiang Yu Mingxing Wang Xunlong Liao Yu Zhao Luquan Ren | 2010 | Journal of Alloys and Compounds2010，，2： | 1 |
| 5 | The influence of film thickness on magnetoresistance and magnetic properties of FeAg granular films显示文摘 | Yu Chengtao Wang Yizhong Lai Wuyan | 1995 | J Magn Magn Mater1995，145，： | 1 |
| 6 | Tantalum coating on porous Ti6Al4V scaffold using chemical vapor deposition and preliminary biological evaluation显示文摘 | Xiang Li Lin Wang Xiaoming Yu Yafei Feng Chengtao Wang Ke Yang Daniel Su | 2013 | Materials Science & Engineering C2013，，5： | 1 |
| 7 | Horizontal transfer of vanA between probiotic Enterococcus faecium and Enterococcus faecalis in fermented soybean meal and in digestive tract of growing pigs显示文摘Background: The aim of this study was to investigate the intergeneric transfer of vancomycin resistance gene vanA between probiotic enterococci in the fermentation progress of soybean meal and in the digestive tract of growing pigs.One vanA genotype vancomycin resistant E.faecium strain,Efm4,and one chloramphenicol-resistant E.faecalis strain,Efs2,were isolated from twenty-nine probiotic basis feed material/additive samples.For in vitro conjugation,Efm4 and Efs2 were used as starter to ferment soybean meal.For in vivo conjugation,thirty growing pigs were randomly assigned to five groups(n = 6),treated with a basic diet,or supplemented with 10% fermented soybean meal,1% Efm4,5% Efs2 or a combination of 1% Efm4 + 5% Efs2 for 7 d,respectively.Fecal samples of pigs in each group were collected daily for the isolation and dynamic analysis of Efm4,Efs2 and transconjugants.The sequence types(STs) of Efm4,Efs2 and transconjugants were analyzed by multilocus sequence typing(MLST).The vanA harboring plasmid in Efm4 and transconjugants was analyzed by S1-pulsed field gel electrophoresis(PFGE)and further verified by multiple alignments.Results: The results showed that,in FSBM,transconjugants were detected 1 h after the fermentation,with a conjugation frequency of ~ 10^-3 transconjugants/recipient.Transconjugants proliferated with Efm4 and Efs2 in the first 8 h and maintained steadily for 10 d till the end of the experiment.Additionally,in vivo experiment showed that transcojugants were recovered in one of six pigs in both FSBM and Efm4 + Efs2 groups,with conjugation frequency of ~ 10^-5 and ~ 10^-4,respectively.MLST revealed the ST of Efm4,Efs2 and transconjugants was ST1014,ST69 and ST69,respectively.S1-PFGE confirmed the existence of the vanA-harboring,142,988-bp plasmid,which was also a multi-drug resistant plasmid containing Tn1546-like transposon.Conclusions: The findings revealed the potential safety hazard existing in the commercial probiotic enterococci in China,because the horizontal transfer from farm to fork could potentially pose a safety risk to the public. | Ning Li Haitao Yu Hongbin Liu Yuming Wang Junyan Zhou Xi Ma Zheng Wang Chengtao Sun Shiyan Qiao | 2019 | Journal of Animal Science and Biotechnology2019，10，3： | 1 |
| 8 | Developmental validation of the novel six-dye Goldeneye^(TM)DNA ID System 35InDel kit for forensic application显示文摘Insertion/deletion polymorphisms(InDels)have been treated as a prospective and helpful genetic marker in the fields of forensic human identification,anthropology and population genetics for the past few years.In this study,we developed a six-dye multiplex typing system consisting of 34 autosomal InDels and Amelogenin for forensic application.The contained InDels were specifically selected for Chinese population with the MAF≥0.25 in East Asia,which do not overlap with the markers of Investigator^(■)DIPplex kit.The typing system was named as GoldeneyeTM DNA ID System 35InDel Kit,and a series of developmental validation studies including repeatability/reproducibility,concordance,accuracy,sensitivity,stability,species specificity and population genetics were conducted on this kit.We confirmed that the 35InDel kit is precise,sensitive,species specific and robust for forensic practice.Moreover,the 35InDel kit is capable of typing DNA extracted from forensic routine case-type samples as well as degraded samples and mixture samples.All markers are proved to be highly polymorphic with an average observed heterozygosity(He)of 0.4582.The combined power of discrimination(CPD)is 0.999999999999978 and the combined power of exclusion in duos(CPE_(D))and trios(CPE_(T))are 0.978837 and 0.999573,respectively,which are higher than those of the Investigator^(■)DIPplex kit.Thus,the GoldeneyeTM DNA ID System 35InDel kit is suitable for forensic human identification and could serve as a supplementary typing system for paternity testing. | Qi Yang Huan Yu Yiling Qu Xiaochun Zhang Ruocheng Xia Ziwei Wang Rui Tan Lei Xiong Shihan Xi Jun Wu Yuzhen Gao Suhua Zhang Chengtao Li | 2022 | Forensic Sciences Research2022，7，4： | 0 |
| 9 | Regulatory variation within 3’UTR of STAT5A correlates with sudden cardiac death in Chinese populations显示文摘Definitive diagnosis to sudden cardiac death(SCD)is often challenging since the postmortem examination on SCD victims could hardly demonstrate an adequate cause of death.It is therefore important to uncover the inherited risk component to SCD.Signal transducer and activators of transcription 5 A(STAT5A)is a member of the STAT family and a transcription factor that is activated by many cell ligands and associated with various cardiovascular processes.In this study,we performed a systematic variant screening on the STAT5A to filter potential functional genetic variations.Based on the screening results,an insertion/deletion polymorphism(rs3833144)in 3’UTR of STAT5A was selected as the candidate variant.A total of 159 SCD cases and 668 SCD matched healthy controls was enrolled to perform a case-control study and evaluate the association between rs3833144 and SCD susceptibility in Chinese populations.Logistic regression analysis showed that the deletion allele of rs3833144 had significantly increased the SCD risk(odds ratio(OR)=1.54;95%confidence interval(CI)=1.18-2.01;P=0.000955).Further genotype-expression eQTL analysis showed that samples with deletion allele appeared to lower expression of STAT5A,and in silico prediction suggested the local 3 D structure changes of STAT5A mRNA caused by the variant.On the other hand,the bioinformatic analysis presented that promoters of RARA and PTGES3L-AARSD1 could interact with rs3833144,and eQTL analysis showed the higher expression of both genes in samples with deletion allele.Dual-luciferase activity assays also suggested the significant regulatory role of rs3833144 in gene transcription.Our current data thus suggested a possible involvement of rs3833144 to SCD predisposition in Chinese populations and rs3833144 with potential function roles may become a candidate marker for SCD diagnosis and prevention. | Huan Yu Yadong Guo Zhenzhen Yang Qing Zhang Jiabin Xu Qi Yang Yiling Qu Rui Tan Lijuan Li Yan He Chengtao Li Suhua Zhang Bin Luo Yuzhen Gao | 2022 | Forensic Sciences Research2022，7，4： | 0 |
| 10 | Stereocomplexed Materials of Chiral Polymers Tuned by Crystallization:A Case Study on Poly(lactic acid)显示文摘CONSPECTUS:Widespread in chemistry and biochemistry,chirality strongly affects the biological,chemical,material,and physical properties.As a downstream derivative,the enantiopure polymers of opposite chirality can cocrystallize in the stereocomplex(SC)in their enantiomeric blends or block copolymers,which endows the materials with improved physical properties and specific functions such as enhanced melting temperature,thermal stability,crystallizability,and hydrolytic resistance. | Ying Zheng Shanshan Xu Chengtao Yu Pengju Pan | 2022 | Accounts of Materials Research2022，3，12： | 0 |
| 11 | Giant Magnetoresistance of Fe/Ag Multilayer Films with Ultrathin Fe Layers显示文摘A giant magnetoresistive effect in as-deposited Fe/Ag multilayer films with ultrathin Fe layers has been observed.The magnetoresistance in this system is presented first as a function of Fe layer thickness tFe.The sample with Fe layer thickness of 4Åexhibits a magnetoresistance ratio as large as 14.3%at 4.2K.The magnetoresistance ratio decreases approximately an order of magnitude with increasing tFe from 4 to 10Åand then changes slightly with further increasing tFe.From the hysteresis loop measurement,it is found that the interlayer coupling changes from antiferromagnetic to ferromagnetic with increasing tFe.The feature of material at the interface might be responsible for all these findings. | LI Shuxiang YU Chengtao YAN Minglang LAI Wuyan WANG Yizhong | 1994 | Chinese Physics Letters1994，11，12： | 0 |
| 12 | Sol-gel derived bioactive hydroxyapatite/titania composite films on Ti6Al4V显示文摘The composite films consisting of the titania gel impregnated with hydroxyapatite (HAP) submicron particles were prepared on commercial Ti6Al4V plates processed by a sol-gel route. HAP powders were synthesized based on wet chemical precipitation method with Ca(NO3)2·4H2O and (NH4)2HPO4 as starting reagents. After being calcined at 900°C,HAP powders were ultrasonically scattered in ethanol to produce HAP sol. The titania sol was prepared using titanium (IV) isopropoxide {Ti[OCH(CH3)2]4} as precursor. Both the titania sol and the HAP/titania mixture were sequentially spin-coated on the substrates and calcined at various temperatures. The characteristics and mechanical adhesion of the composite films were investigated. The results show that the as-prepared films are dense,homogeneous,well-crystallized,and there is a good interfacial adhesion between the film and the substrate. The in vitro bioac-tivities of these films were discussed based on the analysis of the variations of Ca and P concentrations in the simulated body fluid and their surface morphologies against immersion time. | Bing Su Guoqing Zhang Xudong Yu Chengtao Wang | 2006 | Journal of University of Science and Technology Beijing2006，13，5： | 0 |
| 13 | Multi-locus identification of Psilocybe cubensis by high-resolution melting(HRM)显示文摘Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin,psilocin and ibotenic acid,etc.The mushrooms containing hallucinogenic components are various,widely distributed and lack of standard to define,which made a great challenge to identification.Traditional identification methods,such as morphology and toxicology analysis,showed shortcomings in old or processed samples,while the DNA-based identification of hallucinogenic mushrooms would allow to identify these samples due to the stability of DNA.In this paper,four primer sets are designed to target Psilocybe cubensis DNA for increasing resolution of present identification method,and the target markers include largest subunit of RNA polymerase II(marked as PC-R1),psilocybin-related phosphotransferase gene(marked as PC-PT),glyceraldehyde 3-phosphate dehydrogenase(marked as PC-3)and translation EF1α(marked as PC-EF).Real-time PCR with high-resolution melting(HRM)assay were used for the differentiation of the fragments amplified by these primer sets,which were tested for specificity,reproducibility,sensitivity,mixture analysis and multiplex PCR.It was shown that the melting temperatures of PC-R1,PC-PT,PC-3 and PC-EF of P.cubensis were(87.93±0.12)℃,(82.21±0.14)℃,(79.72±0.12)℃ and(80.11±0.19)℃ in our kinds of independent experiments.Significant HRM characteristic can be shown with a low concentration of 62.5pg/µL DNA sample,and P.cubensis could be detected in mixtures with Homo sapiens or Cannabis sativa.In summary,the method of HRM analysis can quickly and specifically distinguish P.cubensis from other species,which could be utilized for forensic science,medical diagnosis and drug trafficking cases. | Xiaochun Zhang Huan Yu Ziwei Wang Qi Yang Ruocheng Xia Yiling Qu Ruiyang Tao Yan Shi Ping Xiang Suhua Zhang Chengtao Li | 2022 | Forensic Sciences Research2022，7，3： | 0 |
| 14 | Magnetic Properties of Compound Fe-Ta-S显示文摘A novel compound Fe-Ta-S crystal has been obtained using iodine vapor transport technology.At low temperature this compound is in a strong diamagnetic state,which is similar to a superconductor.The dependence of the diamagnetic phase transition temperature on the magnetic field could be expressed as T_(c)=Tc0+γH^(2/5)due to a strong coupling between electrons and the magnetic field. | DAI Shouyu YU Chengtao JIN Jiucheng FAN Shiyong | 1995 | Chinese Physics Letters1995，12，1： | 0 |
| 15 | Validation studies of the Para DNA^(■) Intelligence System with artificial evidence items显示文摘Short tandem repeat(STR)profiling is one of the mostly used systems for forensic applications.In certain circumstances,STR profiling is time-consuming and costly,which potentially leads to delays in criminal investigations.LGC(Laboratory of the Government Chemist,UK)Forensics has developed a robust STR profiling platform called the ParaDNAVR Intelligence Test System which can provide early tactical intelligence and aid investigators in making informed decisions on sample prioritization for detection.Here,we validated the ParaDNA^(■) intelligence test for its application in forensic cases using a range of mock evidence items following guidelines set by the Scientific Working Group on DNA Analysis Methods(SWGDAM).Specifically,we tested the sensitivity and accuracy of the ParaDNA intelligence test,as well as the success rates for detecting mock samples and for use in case scenarios.Our findings demonstrate that the ParaDNA intelligence test generates useful DNA profiles,especially for samples such as blood,saliva,and semen that contain ample DNA,indicating the benefits of including ParaDNA as a prior step in forensic STR profiling pipelines. | Min Li Ruiyang Tao Wei Zhou Yanan Li Meng Meng Yilun Zhang Linsheng Yu Liqin Chen Yingnan Bian Chengtao Li | 2021 | Forensic Sciences Research2021，6，1： | 0 |
| 16 | Magnetic Properties of lT-TaS_(2)显示文摘The magnetic properties of 1T-TaS_(2) and 1T-Fe_(0.07)Ta_(0.93)S_(2) have been studied.Experimental results show that the phase transition temperature of IT-TaS_(2) is a function of the magnetic field.At low temperatures,both compounds are in a mixed state of charge density wave-spin density wave due to the coherent superposition of antifer-romagnetic coupling. | DAI Shouyu YU Chengtao LI Donghong FAN Shiyong JIN Jiucheng CAI Fengxin | 1995 | Chinese Physics Letters1995，12，10： | 0 |
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