| 1 | Functional identification of lncRNAs in sweet cherry(Prunus avium)pollen tubes via transcriptome analysis using single-molecule long-read sequencing显示文摘Sweet cherry(Prunus avium)is a popular fruit with high nutritional value and excellent flavor.Although pollen plays an important role in the double fertilization and subsequent fruit production of this species,little is known about its pollen tube transcriptome.In this study,we identified 16,409 transcripts using single-molecule sequencing.After filtering 292 transposable elements,we conducted further analyses including mRNA classification,gene function prediction,alternative splicing(AS)analysis,and long noncoding RNA(lncRNA)identification to gain insight into the pollen transcriptome.The filtered transcripts could be matched with 3,438 coding region sequences from the sweet cherry genome.GO and KEGG analyses revealed complex biological processes during pollen tube elongation.A total of 2043 AS events were predicted,7 of which were identified in different organs,such as the leaf,pistil and pollen tube.Using BLASTnt and the Coding-Potential Assessment Tool(CPAT),we distinguished a total of 284 lncRNAs,among which 154 qualified as natural antisense transcripts(NATs).As the NATs could be the reverse complements of coding mRNA sequences,they might bind to coding sequences.Antisense transfection assays showed that the NATs could regulate the expression levels of their complementary sequences and even affect the growth conditions of pollen tubes.In summary,this research characterizes the transcripts of P.avium pollen and lays the foundation for elucidating the physiological and biochemical mechanisms underlying sexual reproduction in the male gametes of this species. | Yang Li Chuanbao Wu Chunsheng Liu Jie Yu Xuwei Duan Wenqi Fan Jing Wang Xiaoming Zhang Guohua Yan Tianzhong Li Kaichun Zhang | 2019 | Horticulture Research2019，6，1： | 1 |
| 2 | Integrating structure and function:mapping the hierarchical spatial heterogeneity of urban landscapes显示文摘Background:Cities are social-ecological systems characterized by remarkably high spatial and temporal heterogeneity,which are closely related to myriad urban problems.However,the tools to map and quantify this heterogeneity are lacking.We here developed a new three-level classification scheme,by considering ecosystem types(level 1),urban function zones(level 2),and land cover elements(level 3),to map and quantify the hierarchical spatial heterogeneity of urban landscapes.Methods:We applied the scheme using an object-based approach for classification using very high spatial resolution imagery and a vector layer of building location and characteristics.We used a top-down classification procedure by conducting the classification in the order of ecosystem types,function zones,and land cover elements.The classification of the lower level was based on the results of the higher level.We used an objectbased methodology to carry out the three-level classification.Results:We found that the urban ecosystem type accounted for 45.3%of the land within the Shenzhen city administrative boundary.Within the urban ecosystem type,residential and industrial zones were the main zones,accounting for 38.4%and 33.8%,respectively.Tree canopy was the dominant element in Shenzhen city,accounting for 55.6%over all ecosystem types,which includes agricultural and forest.However,in the urban ecosystem type,the proportion of tree canopy was only 22.6%because most trees were distributed in the forest ecosystem type.The proportion of trees was 23.2% in industrial zones,2.2%higher than that in residential zones.That information“hidden”in the usual statistical summaries scaled to the entire administrative unit of Shenzhen has great potential for improving urban management.Conclusions:This paper has taken the theoretical understanding of urban spatial heterogeneity and used it to generate a classification scheme that exploits remotely sensed imagery,infrastructural data available at a municipal level,and object-based spatial analysis.For effective planning and management,the hierarchical levels of landscape classification(level 1),the analysis of use and cover by urban zones(level 2),and the fundamental elements of land cover(level 3),each exposes different respects relevant to city plans and management. | Yuguo Qian Weiqi Zhou Steward T.A.Pickett Wenjuan Yu Dingpeng Xiong Weimin Wang Chuanbao Jing | 2020 | Ecological Processes2020，9，1： | 1 |
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