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| 1 | Mutation of the PHYTOENE DESATURASE 3 gene causes yellowish-white petals in Brassica napus显示文摘Oilseed rape (Brassica napus) with yellow flowers is an attractive ornamental landscape plant during the flowering period,and the development of different petal colors has become a breeding objective.Although yellowish flower color is a common variant observed in field-grown oilseed rape,the genetics behind this variation remains unclear.We obtained a yellowish-white flower (ywf) mutant from Zhongshuang 9 (ZS9) by ethyl methanesulfonate mutagenesis (EMS) treatment.Compared with ZS9,ywf exhibited a lower carotenoid content with a reduced and defective chromoplast ultrastructure in the petals.Genetic analysis revealed that the yellowish-white trait was controlled by a single recessive gene.Using bulked-segregant analysis sequencing (BSA-seq) and kompetitive allele-specific PCR(KASP),we performed map-based cloning of the ywf locus on chromosome A08 and found that ywf harbored a C-to-T substitution in the coding region,resulting in a premature translation termination.YWF,encoding phytoene desaturase 3 (PDS3),was highly expressed in oilseed rape petals and involved in carotenoid biosynthesis.Pathway enrichment analysis of the transcriptome profiles from ZS9 and ywf indicated the carotenoid biosynthesis pathway to be highly enriched.Further analyses of differentially expressed genes and carotenoid components revealed that the truncated Bna A08.PDS3 resulted in decreased carotenoid biosynthesis in the mutant.These results contribute to an understanding of the carotenoid biosynthesis pathway and manipulation of flower-color variation in B.napus. | Chuanji Zhao Luqman Bin Safdar Meili Xie Meijuan Shi Zhixue Dong Li Yang Xiaohui Cheng Yueying Liu Zetao Bai Yang Xiang Chaobo Tong Junyan Huang Lijiang Liu Shengyi Liu | 2021 | The Crop Journal2021，9，5： | 3 |
| 2 | Mapping the topography and cone morphology of the Dalinor volcanic swarm in Inner Mongolia with remote sensing and DEM Data显示文摘Dalinor 暴烈的群, Xilinhot 的定位南方,中国的内部蒙古,是自从晚第三纪时期,发生了的多级式的爆发的结果。这群主要由暴烈的锥和熔岩台地组成。这研究的目的是印射这暴烈的群的地形学和形态学。它基于从各种各样的来源收集的许多数据,例如数字举起模型(DEM ) , Landsat 图象,和 1:50,000 地志的地图除了各种各样的软件平台,为数据处理和解释包括 ArcGIS, Envi4.8,全球 Mapper,和 Google 土。结果证明暴烈的群的全面地形学是一个平台与一中央胀大有大波动,相当大的坡度变化,不平的表面,和小地面地势。根据线侧面的波动的特征,暴烈的群能与 1,280 m, 1,360 m, 1,440 m,和 1,500 m 的高度被划分成四座楼梯。一行侧面的分析与不同高度范围和进化描绘火山的二簇。熔岩台地和暴烈的锥将近是分布式的在里面趋势 EW 系上带子,在台地范围与坡度的迭加的层和地势的度被描出的地方。根据形态学,暴烈的锥被分类进四种类型:圆锥形,合成,屋顶,和盾。暴烈的锥的每种类型的形成原因和分类基础被分析,他们的参数被提取。暴烈的锥的所有类型的 H/D 比率然后统计上被决定并且投射了创造暴烈的密度分发的一张地图。基于在分发和不同的暴烈的锥的形成的时间顺序之间的关系,暴烈的爆发从边缘移居了到熔岩高原的中心,这能被推断。中央区域通过多级式的暴发材料的重叠被形成。另外,很多早盾火山在边缘上被散布。暴烈的锥的词法分析揭示锥的不同类型的进化阶段。从差错的解释 geomorphological 指示物,例如表面陡坡,暴烈的锥的模式,和坑专业的安排削减,它能被推断那趋势 NE 并且将近趋势 EW 差错与火山的形成和分发有关仔细在这个区域是在场的,它是。 | Liwen GONG Ni LI Qicheng FAN Yongwei ZHAO Liuyi ZHANG Chuanjie ZHANG | 2016 | Frontiers of Earth Science2016，10，3： | 3 |
| 3 | Association between fluid-attenuated inversion recovery vascular hyperintensity and outcome varies with different lesion patterns in patients with intravenous thrombolysis显示文摘Background and purpose To evaluate relationship between fluid-attenuated inversion recovery vascular hyperintensity(FVH)after intravenous thrombolysis and outcomes in different lesion patterns on diffusion-weighted imaging(DWI).Methods Patients with severe internal carotid or intracranial artery stenosis who received intravenous thrombolysis from March 2012 to April 2019 were analysed.They were divided into four groups by DWI lesion patterns:border-zone infarct(BZ group),multiple lesions infarct(ML group),large territory infarct(LT group),and single cortical or subcortical lesion infarct(SL group).Logistic regression was performed to identify risk factors for outcome(unfavourable outcome,modified Rankin Scale(mRS)≥2;poor outcome,mRS≥3).Results Finally,203 participants(63.3±10.2 years old;BZ group,n=72;ML group,n=64;LT group,n=37;SL group,n=30)from 1190 patient cohorts were analysed.After adjusting for confounding factors,FVH(+)was associated with unfavourable outcome in total group(OR 3.02;95%CI 1.49 to 6.13;p=0.002),BZ group(OR 4.22;95%CI 1.25 to 14.25;p=0.021)and ML group(OR 5.44;95%CI 1.41 to 20.92;p=0.014)patients.FVH(+)was associated with poor outcome in total group(OR 2.25;95%CI 1.01 to 4.97;p=0.046),BZ group(OR 5.52;95%CI 0.98 to 31.07;p=0.053)and ML group(OR 4.09;95%CI 1.04 to 16.16;p=0.045)patients,which was marginal significance.FVH(+)was not associated with unfavourable or poor outcome in LT and SL groups.Conclusion This study suggests that association between FVH and outcome varies with different lesion patterns on DWI.The presence of FVH after intravenous thrombolysis may help to identify patients who require close observations in the hospitalisation in patients with border-zone and multiple lesion infarcts. | Erling Wang Chuanjie Wu Dandan Yang Xihai Zhao Jie Zhao Hong Chang Qi Yang | 2021 | Stroke & Vascular Neurology2021，6，3： | 3 |
| 4 | De novo design of future rapeseed crops:Challenges and opportunities显示文摘To address the global demand for rapeseed while considering farmers’profit,we face the challenges of making a quantum leap in seed yield and,at the same time,reducing yield loss due to biotic and abiotic stresses.We also face the challenge of efficiently applying new transformative biotechnology tools such as gene editing and breeding by genome design to increase rapeseed productivity and profitability.In this Perspective,we review advances in research on the physiological and genetic bases of both stress factorsaffected yield stability and seed yield potential,focusing on source–sink relationships and allocation of photosynthetic assimilates to vegetative growth and seed development.We propose research directions and highlight the role of plant architecture in the relative contributions of the root system,leaves,and pods to seed yield.We call for de novo design of new rapeseed crops.We review trait variation in existing germplasm and biotechnologies available for crop design.Finally,we discuss opportunities to apply fundamental knowledge and key germplasm to rapeseed production and propose an ideotype for de novo design of future rapeseed cultivars. | Shengyi Liu Harsh Raman Yang Xiang Chuanji Zhao Junyan Huang Yuanyuan Zhang | 2022 | The Crop Journal2022，10，3： | 2 |
| 5 | A delta-class glutathione transferase from the Chinese mitten crab Eriocheir sinensis : cDNA cloning, characterization and mRNA expression显示文摘 | Daxian Zhao Liqiao Chen Chuanjie Qin Hao Zhang Ping Wu Fengying Zhang | 2010 | Fish and Shellfish Immunology2010，，4： | 1 |
| 6 | Flame retardancy and pyrolysis products of calcium alginate fibers 显示文摘 | Chuanjie Zhang Ping Zhu Jinchao Zhao | 2012 | Advanced Materials Research2012，441，： | 1 |
| 7 | The development of a high-speed lower-limb robotic exoskeleton显示文摘Dear editor,With continuously advancing science and technology, the lower-limb robotic exoskeleton, an assistive-type device for humans, has achieved unprecedented development in both the military and civilian fields. At present, several exoskeletons have entered the commercialization stage. | Zhi REN Chuanjie DENG Kuankuan ZHAO Zhijun LI | 2019 | Science China(Information Sciences)2019，62，5： | 0 |
| 8 | Corrigendum to“De novo design of future rapeseed crops:Challenges and opportunities”[Crop J.10(2022)587-596]显示文摘The authors regret to report some mistakes in the text and associated changes necessary to section 4.1 of the paper.On page 591 in the right-hand column,line 34,“far light”should be changed to“far-red light”.On page 592 in the left-hand column,line 28,the sentence reads:“If the YP/FP ratio can be as high as 42%(the average YP/FP of the UK and China.Table S2),the farm yield will be 5.0 t ha1.” | Shengyi Liu Harsh Raman Yang Xiang Chuanji Zhao Junyan Huang Yuanyuan Zhang | 2022 | The Crop Journal2022，10，4： | 0 |
| 9 | Single-cell level point mutation analysis of circulating tumor cells through droplet microfluidics显示文摘Tumor heterogeneity plays a critical role in the determination of appropriate anticancer therapy.As cir-culating tumor cells(CTCs)contain all tumor-related information,the genetic changes on CTCs could help us choose the appropriate treatments for different patients.Single-base mutations are very common in tumor genetic changes which may result in drug resistance.Here,we introduce a single-cell mutation de-tection platform based on droplet microfluidics.This platform integrates cell capsulation,cell lysis,poly-merase chain reaction(PCR)and the observation process.The droplets’generation speed is over 6000 per minute and more than 600 cells could be encapsulated in one second.To verify the performance of our platform in practical use,we performed the mutation analysis of 4 kinds of cells with our platform and noted that the genetic status of each single cell was clearly discriminated.Moreover,these results agreed with those from direct sequencing.Compared with other forms of single-cell mutation detection techniques,our platform has high throughput,short experimental time and less experimental operations. | Shihui Qiu Chuanjie Shen Xiaoyu Jian Yunxing Lu Zhaoduo Tong Zhenhua Wu Hongju Mao Jianlong Zhao | 2022 | Chinese Chemical Letters2022，33，5： | 0 |
| 10 | Genome-wide association study exposed the pleiotropic genes for yield-related and oil quality traits in Brassica napus L.显示文摘Oilseed rape(Brassica napus L.) is an allotetraploid(AACC,2n=38) crop,valued for its edible oil and protein content.seed yield and nutritional composition of rapeseed are influenced by its yield and oil quality traits.However,the genetic basis of yield-related and oil-quality traits remain ambiguous.A panel of 266 diversified oilseed rape accessions was genotyped using 223 simple sequence repeat(SSR) markers covering all 19 chromosomes to identify significant markers associated with yield and quality traits.Twelve yield-related and six quality traits were investigated in two consecutive years(2014 and 2015),with three replications in two environments(Changshun,CS;and Qinghe,QH).Using the model GLM with population structure and kinship(Q+K),a total of 25 significant SSR markers(P <0.001) were detected to be associated with these twelve yield-related and six quality traits,explaining 4.56%-19.17% of the phenotypic variation for each trait.Based on these markers,BnaA03g23490D, BnaC09g46370D,BnaA07g37150D,BnaA01g32590D, and BnaC09g37280D were identified as pleiotropic genes controlling multiple traits.These candidate genes illustrated the potential for the genetic understanding of yield and oil quality traits.Most importantly,these significant markers can be used for marker-assisted breeding of oilseed rape in different environments. | Yang Xiang M.M.U.Helal Longbing Liang Minqin Zhang Hongshi Han Xianglai Dai Jixian Zhao Dalun Chen Xianping Wang Min Li Chuanji Zhao | 2023 | Oil Crop Science2023，8，3： | 0 |
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