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| 1 | Optical analysis of a hybrid solar concentrating Photovoltaic /Thermal (CPV/T) system with beam splitting technique显示文摘A novel hybrid solar concentrating Photovoltaic/Thermal (CPV/T) system with beam splitting technique is presented. In this system, a beam splitter is used to separate the concentrated solar radiation into two parts: one for the PV power generation and the other for thermal utility. The solar concentrator is a flat Fresnel-type concentrator with glass mirror reflectors. It can concentrate solar radiation onto solar cells with high uniformity, which is beneficial to improving the efficiency of solar cells. The thermal receiver is separated to the solar cells, and therefore, the thermal fluid can be heated to a relatively high temperature and does not affect the performance of solar cells. A dimensionless model was developed for the performance analysis of the concentrating system. The effects of the main parameters on the performance of the concentrator were analyzed. The beam splitter with coating materials Nb2O3 /SiO2 was designed by using the needle optimization technique, which can reflect about 71% of the undesired radiation for silicon cell(1.1m < 3m) to the thermal receiver for thermal utility. The performance of this CPV/T system was also theoretically analyzed. | HU Peng ZHANG Qian LIU Yang SHENG ChunChen CHENG XiaoFang CHEN ZeShao | 2013 | Science China(Technological Sciences)2013，56，6： | 10 |
| 2 | Productive HBV infection of well-differentiated, hNTCP-expressing human hepatoma-derived(Huh7) cells显示文摘Feasible and effective cell models for hepatitis B virus(HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol(DMSO/PEG), h NTCP expression, and a differentiated state are the limiting factors for successful HBV infection models. In the present study, we used a hepatoma cell line(Hu7^(hDNTCPh)) to overcome these limiting factors so that it exhibits excellent susceptibility to HBV infection. To achieve this goal, different hepatoma cell lines were tested with 2.5% DMSO/4%PEG8000, and one resistant cell line(Huh7 D) was used to construct a stable h NTCP-expressing cell line(Hu7^(hDNTCPh)) using a recombinant lentivirus system. Then, the morphological characteristics and differentiation molecular markers of Hu7^(hDNTCPh) cells with or without DMSO treatment were characterized. Finally, the susceptibility of Hu7^(hDNTCPh) cells to HBV infection was assessed. Our results showed that Huh7 D cells were resistant to 2.5% DMSO/4% PEG8000, whereas the others were not. Hu7^(hDNTCPh) cells were established to express a high level of h NTCP compared to liver extracts, and Hu7^(hDNTCPh) cells rapidly transformed into a non-dividing, well-differentiated polarized phenotype under DMSO treatment. Hu7^(hDNTCPh) cells fully supported the entire lifecycle of HBV infection. This cell culture system will be useful for the analysis of host-virus interactions, which should facilitate the discovery of antiviral drugs and vaccines. | Ming Zhou Kaitao Zhao Yongxuan Yao Yifei Yuan Rongjuan Pei Yun Wang Jizheng Chen Xue Hu Yuan Zhou Xinwen Chen Chunchen Wu | 2017 | Virologica Sinica2017，32，6： | 7 |
| 3 | Phosphatidylserine-Specific Phospholipase A1 is the Critical Bridge for Hepatitis C Virus Assembly显示文摘The phosphatidylserine-specific phospholipase A1(PLA1A)is an essential host factor in hepatitis C virus(HCV)assembly.In this study,we mapped the E2,NS2 and NS5A involved in PLA1A interaction to their lumenal domains and membranous parts,through which they form oligomeric protein complexes to participate in HCV assembly.Multiple regions of PLA1A were involved in their interaction and complex formation.Furthermore,the results represented structures with PLA1A and E2 in closer proximity than NS2 and NS5A,and strongly suggest PLA1 A-E2,s physical interaction in cells.Meanwhile,we mapped the NS5A sequence which participated in PLA1A interaction with the C-terminus of domain 1.Interestingly,these amino acids in the sequence are also essential for viral RNA replication.Further experiments revealed that these four proteins interact with each other.Moreover,PLA1A expression levels were elevated in livers from HCV-infected patients.In conclusion,we exposed the structural determinants of PLA1A,E2,NS2 and NS5A proteins which were important for HCV assembly and provided a detailed characterization of PLA1A in HCV assembly. | Qi Yang Min Guo Yuan Zhou Xue Hu Yun Wang Chunchen Wu Min Yang Rongjuan Pei Xinwen Chen Jizheng Chen | 2019 | Virologica Sinica2019，34，5： | 4 |
| 4 | Host HDAC4 regulates the antiviral response by inhibiting the phosphorylation of IRF3显示文摘Class II HDACs, such as HDAC4, are critical regulators of the immune response in various immune cells;however, its role in innate immunity remains largely unknown.Here, we report that the overexpression of HDAC4 suppresses the production of type I interferons triggered by pattern-recognition receptors (PRRs). HDAC4 repressed the translocation of transcription factor IRF3 to the nucleus, thereby decreasing IRF3-mediated IFN-β expression. In particular, we also determined that HDAC4 can be phosphorylated and simultaneously block the phosphorylation of IRF3 at Ser386 and Ser396 by TBK1 and IKKε, respectively, by interacting with the kinase domain of TBK1 and IKKε. Furthermore, IFN-β may stimulate the expression of HDAC4. Our findings suggest that HDAC4 acts as a regulator of PRR signaling and is a novel mechanism of negative feedback regulation for preventing an overreactive innate immune response. | Qi Yang Jielin Tang Rongjuan Pei XiaoXiao Gao Jing Guo Chonghui Xu Yun Wang QianWang Chunchen Wu Yuan Zhou Xue Hu He Zhao Yanyi Wang Xinwen Chen Jizheng Chen | 2019 | Journal of Molecular Cell Biology2019，11，2： | 2 |
| 5 | High brightness NIR-Ⅱ nanofluorophores based on fused-ring acceptor molecules显示文摘It is challenging to develop molecular fluorophores in the second near-infrared(NIR-Ⅱ)window with long wavelength emission and high brightness,which can improve the performance of biological imaging.Herein,we report a molecular engineering approach to afford NIR-Ⅱ fluorophores with these merits based on fused-ring acceptor(FRA)molecules.Dioctyl 3,4-propylenedioxy thiophene(PDOT-C8)is utilized as the bridging donor to replace 3-ethylhexyloxy thiophene(3-EHOT),leading to more than 20 times enhancement of brightness.The nanofluorophores(NFs)based on the optimized CPTIC-4F molecule exhibit an emission peak of 1,110 nm with a fluorescence quantum yield(QY)of 0.39%(QY of IR-26 is 0.050%in dichloroethane as reference)and peak absorption coefficient of 14.5 x 10^4 M^-1·cm^-1 in aqueous solutions,which are significantly higher than those of 3-EHOT based COTIC-4F NFs.It is found that PDOT-C8 can weaken intermolecular aggregation,enhance protection of molecular backbone from water,and decrease backbone distortion,beneficial for the high brightness.Compared with indocyanine green with same injection dose,CPTIC-4F NFs show 10 times higher signal-to-background ratio for whole body vessels imaging at 1,300 nm long pass filters. | Xingfu Zhu Chunchen Liu Zhubin Hu Haile Liu Jiang Wang Yang Wang Xinyuan Wang Rui Ma Xiaodong Zhang Haitao Sun Yongye Liang | 2020 | Nano Research2020，13，9： | 1 |
| 6 | Discovery of a potent and selective cell division cycle 7 inhibitor from 6-(3-fluoropyridin4-yl)thieno[3,2-d]pyrimidin-4(3H)-one derivatives as an orally active antitumor agent显示文摘To the Editor:Kinase cell division cycle 7(CDC7),a cell division cycle protein,takes a vital role in mediating DNA replication1.CDC7 complexes in the nucleus can phosphorylate the minichromosome maintenance complex(MCM)family members that bind to chromosomes.In addition,CDC7 kinase,as a molecular switch regulating DNA replication,can mediate DNA damage signaling pathways to stimulate cell cycle termination as well as DNA replication2.Studies have shown that CDC7 is overexpressed in many types of cancer cells,and its overexpression was related to poor patient survival,tumor grade,genetic instability,aneuploidy and so on3.Therefore,CDC7 is a promising target for antitumor therapy. | Mingwei Fu Min Ge Wanxiang Yang Chunchen Hu Xiaowei Li Yuanjiang Wang Shaohua Gou | 2024 | Acta Pharmaceutica Sinica B2024，14，2： | 0 |
| 7 | Furan Donor for NIR-II Molecular Fluorophores with Enhanced Bioimaging Performance显示文摘The second near-infrared(NIR-II,1,000 to 1,700 nm)molecular fluorophores containing donor–acceptor–donor conjugated backbone have attracted substantial attention due to their outstanding advantages,such as stable emission and facilely tuned photophysical properties.However,it is still challenging for them to simultaneously achieve high brightness and red-shifted absorption and emission.Herein,furan is adopted as the D unit to construct NIR-II fluorophores,demonstrating red shift of absorption,enhanced absorption coefficient,and fluorescent quantum yield when compared with the generally used thiophene counterparts.The high brightness and desirable pharmacokinetics of the optimized fluorophore,IR-FFCHP,endows improved performance for angiography and tumor-targeting imaging.Furthermore,dual-NIR-II imaging of tumor and sentinel lymph nodes(LNs)has been achieved with IR-FFCHP and PbS/CdS quantum dots,enabling the in vivo imaging navigated LN surgery in tumor-bearing mice.This work demonstrates the potential of furan for constructing bright NIR-II fluorophores for biological imaging. | Chunchen Liu Mengfei Li Huilong Ma Zhubin Hu Xinyuan Wang Rui Ma Yingying Jiang Haitao Sun Shoujun Zhu Yongye Liang | 2023 | Research2023，，3： | 0 |
| 8 | Repurposing of Antazoline Hydrochloride as an Inhibitor of Hepatitis B Virus DNA Secretion显示文摘Hepatitis B virus(HBV) belongs to Hepadnaviridae family and mainly infects hepatocytes, which can cause acute or chronic hepatitis. Currently, two types of antiviral drugs are approved for chronic infection clinically: interferons and nucleos(t)ide analogues. However, the clinical cure for chronic infection is still rare, and it is a huge challenge for all researchers to develop high-efficiency, safe, non-tolerant, and low-toxicity anti-HBV drugs. Antazoline hydrochloride is a first-generation antihistamine with anticholinergic properties, and it is commonly used to relieve nasal congestion and in eye drops. Recently, an in vitro high-throughput evaluation system was constructed to screen nearly 800 compounds from the Food and Drug Administration(FDA)-approved Drug Library. We found that arbidol hydrochloride and antazoline hydrochloride can effectively reduce HBV DNA in the extracellular supernatant in a dose-dependent manner, with EC_(50) of4.321 lmol/L and 2.910 lmol/L in HepAD38 cells, respectively. Moreover, the antiviral effects and potential mechanism of action of antazoline hydrochloride were studied in different HBV replication systems. The results indicate that antazoline hydrochloride also has a significant inhibitory effect on HBV DNA in the extracellular supernatant of Huh7 cells,with an EC_(50) of 2.349 lmol/L. These findings provide new ideas for screening and research related to HBV agents. | Jing Li Yangyang Hu Yifei Yuan Yinan Zhao Qiqi Han Canyu Liu Xue Hu Yuan Zhou Yun Wang Yu Guo Chunchen Wu Xinwen Chen Rongjuan Pei | 2021 | Virologica Sinica2021，36，3： | 0 |
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