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| 1 | The model of cytokine release syndrome in CAR T-cell treatment for B-cell non-Hodgkin lymphoma显示文摘Chimeric antigen receptor T(CAR T)cell therapy has demonstrated efficacy in the treatment of haematologic malignancies.However,the accompanying adverse events,the most common of which is cytokine release syndrome(CRS),substantially limit its wide application.Due to its unique physiological characteristics,CRS in CAR T-cell treatment for B-cell non-Hodgkin lymphoma(BNHL)may exhibit some special features.Although existing guidelines had greatly promoted the recognition and management of CRS,many recommendations are not fully applicable to B-NHL.Therefore,it is imperative to identify responses that are specific to CRS observed following CAR T treatment for B-NHL.Based on underlying biological processes and known pathophysiological mechanisms,we tentatively propose a new model to illustrate the occurrence and evolution of CAR T-cell-therapy-related CRS in BNHL.In this model,tumour burden and bone marrow suppression are considered determinants of CRS.Novel phenomena after CAR T-cell infusion(such as local inflammatory response)are further identified.The proposed model will help us better understand the basic biology of CRS and recognize and manage it more rationally. | Jianshu Wei Yang Liu Chunmeng Wang Yajing Zhang Chuan Tong Guanghai Dai Wei Wang John E.J.Rasko J.Joseph Melenhorst Wenbin Qian Aibin Liang Weidong Han | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 2 | A quantitative assessment on groundwater salinization in the Tarim River lower reaches,Northwest China显示文摘Based on monitored data from 840 samples,we assessed the spatial and temporal variability of groundwater salinization in the Tarim River lower reaches combining classical statistics and geostatistics.Results show that total dissolved solids(TDS)is significantly correlated with other related ions,such as Na+,Mg2+,Ca2+,Cl and K+.TDS and underground water level have characteristics of spatial autocorrelation,both of which present the isotropic characteristic and conform to the spherical model in each year from 2001–2009.TDS is basically greater than 1 g/L but less than 2 g/L in the Tarim River lower reaches,which indicates that salt stagnation pollution is more serious.The most serious salinization(3 g/L | JianHua Xu WeiHong Li YuLian Hong ChunMeng Wei Jie Tang | 2014 | Research in Cold and Arid Regions2014，6，1： | 1 |
| 3 | The gene expression level and prognosis analysis of membrane protein AP2M1 based on the protein spectrum of exosomes in hepatocellular carcinoma cells显示文摘Objective:Adopting mass spectrometry to analyze the protein spectrum of the exosomes in human hepatocellular carcinoma cells and screening the target membrane protein in order to analyze the relationship between its gene expression level and prognosis.Methods:The exosomes of hepatocellular carcinoma cells HepG2 and 7721 were isolated by ultracentrifugation,and the extracted exosomes were verified by transmission electron microscope, western blotting,and nanosight.The relative quantitative proteomics analysis was used to analyze the protein spectrum of HepG2 and 7721 cells in exosomes.The bioinformatics was used to collect the signal pathway,and the target protein AP2M1 was verified by qRTPCR.Gene Expression Profiling Interactive Analysis(GEPIA),OncoLnc,and The Cancer Genome Atlas(TCGA)database were used to analyze the expression level of AP2M1 and the survival rate of patients with liver cancer.Results:The extracellular exosomes of HepG2 and 7721 cells were isolated by ultracentrifugation.High-purity exosomes were obtained and verified by transmission electron microscopy,western blotting,and nanosight.There were 836 proteins co-expressed by the exosomes of HepG2 and 7721.By the analysis of Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway,a total of 34 pathways that were related to liver cancer were collected,and the target membrane protein AP2M1 was screened.AP2M1 highly expressed in HepG2 cells,HepG2 and Huh7 cells' exosomes were detected by qRT-PCR(P<0.05).The data of GEPIA showed that comparing to the adjacent tissues,gene AP2M1 was highly expressed in hepatocarcinoma tissues.Kaplan-Meier survival curve analysis was performed by OncoLnc.It was found that the survival rate of patients with liver cancer and high expression of AP2M1 was significantly lower than patients with liver cancer and low expression of AP2M1(P<0.01).Conclusion:The high expression of membrane protein AP2M1 in the exosomes of hepatoma cells and the high expression of its gene in liver cancer tissues predicted the low survival rate. | Lifang Liang Chunmeng Wei Yasi Li Fengjie Wan Xuejing Huang Min He | 2018 | 广西医科大学学报2018，35，11： | 1 |
| 4 | The correlations between the expression of FGFR4 protein and clinicopathological parameters as well as prognosis of gastric cancer patients显示文摘 | Yan‐Wei Ye Xiefu Zhang Ye Zhou Jianghong Wu Chunlin Zhao Lin Yuan Guojun Wang Chunyan Du Chunmeng Wang Yingqiang Shi | 2012 | Oncol2012，，7： | 1 |
| 5 | Characteristics of premanufacture CD8^(+) T cells determine CAR-T efficacy in patients with diffuse large B-cell lymphoma显示文摘Although chimeric antigen receptor(CAR)T cells have become an important treatment option for patients with relapsed/refractory B-cell malignancies,more than 60%of patients with diffuse large B-cell lymphoma(DLBCL)treated with CAR-T cell therapies fail to achieve a durable response.To reveal changes in CAR-T cell therapy and identify response biomarkers,we conducted a retrospective analysis of pre-manufacture source T cells and CAR-T cell products and their association with outcome in 58 patients with r/rDLBCL who received tandem CD19/CD20 CAR-T cell therapy.We performed bulk RNA-Seq,single-cell RNA-Seq,and paired T cell receptor sequencing on CAR-T cell products and pre-manufacture T cells from DLBCL patients.We note that a CD8+stem cell-like memory T cell population with a higher proportion and enhanced activating capacity of the CAR-T cell products was key to achieving durable clinical response.By analysing autologously-derived,pre-manufacture T cells,our data suggest that heterogeneity in the cellular and molecular features of pre-manufacture T cells contribute to the variation in efficacy after CAR-T cell therapy in DLBCL.The differences in anti-tumour efficacy of CAR-T cells among patients with different clinical outcomes appear to be due to the loss of CCR7 gene expression,coupled with increased expression of activation-and inhibitor-related genes in the CD8+naïve-T cell populations among the apheresis T cells from patients with a poor molecular response.These findings significantly advance our understanding of the underlying molecular determinants of pre-manufacture T cell function. | Yao Wang Chuan Tong Yuting Lu Zhiqiang Wu Yelei Guo Yang Liu Jianshu Wei Chunmeng Wang Qingming Yang Weidong Han | 2023 | Signal Transduction and Targeted Therapy2023，8，11： | 0 |
| 6 | Treatment and prognosis of iliac fossa soft tissue sarcoma: A single-center study显示文摘Dear Editor,Primary iliac fossa sarcoma(IFS)is a special type of retroperitoneal sarcoma(RPS),accounting for∼15%of all RPS cases[1].The deep location,large size,and invasion to surrounding tissues and organs are the main causes of unresectability of IFS.All these characteristics are associated with an increased risk of positive surgical margin and a decreased feasibility of adjuvant therapy.In patients with multivisceral and/or vascular involvement,a multivisceral en bloc approach[2]with blood vessel replacement may be required to achieve a negative margin and to improve the quality of resection[3].However,whether these surgical procedures improve prognosis in patients with IFS remain undefined.Moreover,whether aggressive procedures lead to acceptable functional impairment requires validation.In addition,previous studies have reported inconsistent results regarding the role of adjuvant radiotherapy in the treatment of RPS[4].To date,the role of radiotherapy in the local control of IFS remains to be determined.Therefore,we analyzed the clinical features,treatment,and outcomes of IFS patients in an attempt to determine the significant prognostic factors and efficient therapeutics in real clinical practice. | Wei Sun Hongqiang Zhang Biqiang Zheng Ruming Zhang Chunmeng Wang Wangjun Yan Bing Wang Xinglong Qu Yong Chen | 2020 | Cancer Communications2020，40，7： | 0 |
| 7 | Bioinformatics analysis of exosome proteome derived from hepatic carcinoma HepG2 cells显示文摘Objective:The aim of this study was to analyze the exosome proteome derived from hepatic carcinoma HepG2 cells and normal hepatocytes HL-7702,and look for the key factors in carcinogenesis of hepatocellular carcinoma(HCC).Methods:HepG2 and HL-7702 cells were cultured,and exosome samples were obtained from the culture supernatant and verified.LTQ-Orbitrap Elite mass spectrometry was applied to analyze and identify the exosome proteome derived from the hepatic carcinoma cells and normal liver cells,which was for seeking hepatic carcinoma cells specifically expressed proteins.Furthermore,gene ontology(GO),protein-protein interaction(PPI)network,and pathway enrichment were constructed to analyze hepatic carcinoma cells specifically expressed proteins.Results:The exosomes of HL-7702 expressed 3,366proteins,and the exosomes of HepG2 expressed 2,874proteins,of which 1,224were expressed specifically in HepG2 exosomes.102 target proteins were selected and going on bioinformatics analysis under the condition that the unique peptide was≥1and PSMs≥10.GO analysis results showed that the target expression protein of HepG2 was concentrated on metabolism,proliferation,and localization adhesion function.76target proteins were chosen and formed a PPI network;combining with pathway analysis,ACTN1,FN1,RAC1and GSN were found to be involved in important signaling pathways of HCC.The 26target proteins which not in PPI network were analyzed pathway involved in,respectively.AKR1C2 and C5 were found to be involved in differently important signaling pathways of HCC.Conclusion:Exosomes proteomics provides the sources of specific protein derived from hepatic carcinoma cell;when it combines with bioinformatics analysis,they are able to reveal the important molecules that are related to carcinogenesis of HCC and provide new ideas for investigation of HCC biomarkers. | Chunmeng Wei Jinghuan Deng Jiagui Chen Fengjie Wan Yasi Li Min He | 2017 | 广西医科大学学报2017，34，3： | 0 |
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