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| 1 | Insights into wing dimorphism in worldwide agricultural pest and hostalternating aphid Aphis gossypii显示文摘Background:The worldwide pest Aphis gossypii has three-winged morphs in its life cycle,namely,winged parthenogenetic female(WPF),winged gynopara(GP),and winged male,which are all produced by a wingless parthenogenetic female(WLPF).Most studies on A.gossypii have focused on WPF,while few have investigated GP and male.The shared molecular mechanism underlying the wing differentiation in the three wing morphs of A.gossypii remains unknown.The wing differentiation of WPF was explored in a previous study.Herein,GP and male were induced indoors.The characters of the body,internal genitals,wing veins,and fecundity of GP and male were compared with those of WPF or WLPF.Compared with WLPF,the shared and separate differentially expressed genes(DEGs)were identified in these three-wing morphs.Results:Newly-born nymphs reared in short photoperiod condition(8 L:16D,18°C)exclusively produced gynoparae(GPe)and males in adulthood successively,in which the sex ratio was GP biased.A total of 14 GPe and 9 males were produced by one mother aphid.Compared with WLPF,the three-wing morphs exhibited similar morphology and wing vein patterns but were obviously discriminated in the length of fore-and underwings,reproductive system,and fecundity.A total of 37090 annotated unigenes were obtained from libraries constructed using the four morphs via RNA sequencing(RNA-Seq).In addition,10867 and 19334 DEGs were identified in the pairwise comparison of GP versus WLPF and male versus WLPF,respectively.Compared with WLPF,the winged morphs demonstrated 2335 shared DEGs(1658 upregulated and 677 downregulated).The 1658 shared upregulated DEGs were enriched in multiple signaling pathways,including insulin,FoxO,MAPK,starch and sucrose metabolism,fatty acid biosynthesis,and degradation,suggesting their key roles in the regulation of wing plasticity in the cotton aphid.Forty-four genes that spanned the range of differential expression were chosen to validate statistical analysis based on RNA-Seq through the reverse transcription quantitative real time polymerase chain reaction(RT-qPCR).The comparison concurred with the expression pattern(either up-or downregulated)and supported the accuracy and reliability of RNA-Seq.Finally,the potential roles of DEGs related to the insulin signaling pathway in wing dimorphism were discussed in the cotton aphid.Conclusions:The present study established an efficiently standardized protocol for GP and male induction in cotton aphid by transferring newly-born nymphs to short photoperiod conditions(8 L:16D,18°C).The external morphological characters,especially wing vein patterns,were similar among WPFs,GPe,and males.However,their reproductive organs were strikingly different.Compared with WLPF,shared(2335)and exclusively(1470 in WLPF,2419 in GP,10774 male)expressed genes were identified in the three-wing morphs through RNA-Seq,and several signaling pathways that are potentially involved in their wing differentiation were obtained,including insulin signaling,starch and sucrose metabolism. | JI Jichao HUANGFU Ningbo LUO Junyu GAO Xueke NIU Lin ZHANG Shuai CUI Jinjie | 2021 | Journal of Cotton Research2021，4，1： | 2 |
| 2 | TG–FTIR and Py–GC/MS analysis on pyrolysis and combustion of pine sawdust显示文摘 | Ningbo Gao Aimin Li Cui Quan Lin Du Yue Duan | 2012 | Journal of Analytical and Applied Pyrolysis2012，，： | 1 |
| 3 | A novel reforming method for hydrogen product-ion from biomass steam gasification显示文摘 | Ningbo Gao Aimin Li Cui Quan | | 0，，： | 1 |
| 4 | Regulated deficit irrigation improved fruit quality and water use efiqciency of pear-jujube trees显示文摘 | Cui Ningbo Du Taisheng Kang Shaozhong | 2008 | Agricultural Water Management2008，95，4： | 1 |
| 5 | TG-FTIR and Py-GC/MS analysis on pyrolysis and combustion of pine sawdust 显示文摘 | Gao Ningbo Li Aimin Quan Cui | 2013 | Journal of Analytical and Applied Pyrolysis2013，100，6： | 1 |
| 6 | Characterization of products recycling from PCB waste pyrolysis显示文摘 | Cui Quan Aimin Li Ningbo Gao Zhang dan | 2010 | Journal of Analytical and Applied Pyrolysis2010，，1： | 1 |
| 7 | Regulated deficit irrigation improved fruit quality and water use efficiency of pear-jujube trees显示文摘 | Cui Ningbo Du Taisheng Kang Shaozhong | 2008 | Agricultural Water Management2008，95，： | 1 |
| 8 | Synthesis of carbon nanotubes and porous carbons from printed circuit board waste pyrolysis oil 显示文摘 | Cui Quan Aimin Li Ningbo Gao | 2010 | Journal of Hazardous Materials2010，179，： | 1 |
| 9 | Response of vegetative growth,fruit development and water use efficiency to regulated deficit irrigation at different growth stages of pear-jujube tree显示文摘 | Ningbo Cui Taisheng Du Fusheng Li | 2009 | Agricultural Water Management2009，96，： | 1 |
| 10 | Thermogravimetric analysis and kinetic study on large particles of printed circuit board wastes显示文摘 | Quan Cui Li Aimin Gao Ningbo | 2009 | Waste Management2009，29，8： | 1 |
| 11 | Thermogravimetric analysis and kinetic study on large particles of printed circuit board wastes显示文摘 | Quan Cui Li Aimin Gao Ningbo | 2009 | Waste Management2009，29，： | 1 |
| 12 | Regulated deficit irrigation improved fruit quality and water use efficiency of pear-jujube trees显示文摘 | Ningbo Cui Taisheng Du Shaozhong Kang Fusheng Li Jianhua Zhang Mixia Wang Zhijun Li | 2007 | Agricultural Water Management2007，，4： | 1 |
| 13 | A novel reforming method for hydrogen production from biomass steam gasification显示文摘 | Gao Ningbo Li Aimin Quan Cui | 2009 | Bioresource Technology Journal2009，100，： | 1 |
| 14 | Regulated deficit irrigation improved fruit quality and water use efficiency of pear-jujube trees显示文摘 | CUI Ningbo DU Taisheng KANG Shaozhong | 2008 | Agriculture Water Manage2008，95，： | 1 |
| 15 | Electron-ion collider in China显示文摘Lepton scattering is an established ideal tool for studying inner structure of small particles such as nucleons as well as nuclei.As a future high energy nuclear physics project,an Electron-ion collider in China(EicC)has been proposed.It will be constructed based on an upgraded heavy-ion accelerator,High Intensity heavy-ion Accelerator Facility(HIAF)which is currently under construction,together with a new electron ring.The proposed collider will provide highly polarized electrons(with a po-larization of 80%)and protons(with a polarization of 70%)with variable center of mass energies from 15 to 20 GeV and the luminosity of(2–3)×1033 cm^(−2)·s^(−1).Polarized deuterons and Helium-3,as well as unpolarized ion beams from Carbon to Uranium,will be also available at the EicC.The main foci of the EicC will be precision measurements of the structure of the nucleon in the sea quark region,including 3D tomography of nucleon;the partonic structure of nuclei and the parton interaction with the nuclear environment;the exotic states,especially those with heavy flavor quark contents.In addition,issues fundamental to understanding the origin of mass could be addressed by measurements of heavy quarkonia near-threshold production at the EicC.In order to achieve the above-mentioned physics goals,a hermetical detector system will be constructed with cutting-edge technologies.This document is the result of collective contributions and valuable inputs from experts across the globe.The EicC physics program complements the ongoing scientific programs at the Jefferson Laboratory and the future EIC project in the United States.The success of this project will also advance both nuclear and particle physics as well as accelerator and detector technology in China. | Daniele PAnderle Valerio Bertone Xu Cao Lei Chang Ningbo Chang Gu Chen Xurong Chen Zhuojun Chen Zhufang Cui Lingyun Dai Weitian Deng Minghui Ding Xu Feng Chang Gong Longcheng Gui Feng-Kun Guo Chengdong Han Jun He Tie-Jiun Hou Hongxia Huang Yin Huang KrešImir KumeričKi LPKaptari Demin Li Hengne Li Minxiang Li Xueqian Li Yutie Liang Zuotang Liang Chen Liu Chuan Liu Guoming Liu Jie Liu Liuming Liu Xiang Liu Tianbo Liu Xiaofeng Luo Zhun Lyu Boqiang Ma Fu Ma Jianping Ma Yugang Ma Lijun Mao Cédric Mezrag HervéMoutarde Jialun Ping Sixue Qin Hang Ren Craig DRoberts Juan Rojo Guodong Shen Chao Shi Qintao Song Hao Sun PawełSznajder Enke Wang Fan Wang Qian Wang Rong Wang Ruiru Wang Taofeng Wang Wei Wang Xiaoyu Wang Xiaoyun Wang Jiajun Wu Xinggang Wu Lei Xia Bowen Xiao Guoqing Xiao Ju-Jun Xie Yaping Xie Hongxi Xing Hushan Xu Nu Xu Shusheng Xu Mengshi Yan Wenbiao Yan Wencheng Yan Xinhu Yan Jiancheng Yang Yi-Bo Yang Zhi Yang Deliang Yao Zhihong Ye Peilin Yin C-PYuan Wenlong Zhan Jianhui Zhang Jinlong Zhang Pengming Zhang Yifei Zhang Chao-Hsi Chang Zhenyu Zhang Hongwei Zhao Kuang-Ta Chao Qiang Zhao Yuxiang Zhao Zhengguo Zhao Liang Zheng Jian Zhou Xiang Zhou Xiaorong Zhou Bingsong Zou Liping Zou | 2021 | Frontiers of physics2021，16，6： | 1 |
| 16 | Hydrogen-rich gas production from biomass steam gasification in an updraft fixed-bed gasifier combined with a porous ceramic reformer显示文摘 | Ningbo G Aimin L Cui Q | 2008 | Intemational Journal of Hydrogen Energy2008，33，20： | 1 |
| 17 | Regulated deficit irrigation improved fruit quality and water use efficiency of pear-ju- jube trees显示文摘 | Ningbo Cui Taisheng Du Shaozhong Kang | 2008 | Agricultural Water Management2008，95，： | 1 |
| 18 | Regulated deficit irrigation improved fruit quality and water use efficiency of pear-jujube trees显示文摘 | Ningbo Cui Taisheng Du Shaozhong Kang | | 0，，04： | 1 |
| 19 | Regu- lated deficit irrigation improved fruit quality and water use efficiency of pear-jujube trees显示文摘 | CUI Ningbo DU Taisheng KANG Shaozhong | 2008 | Agriculture Water Man- age2008，95，4： | 1 |
| 20 | Relation- ship between stable carbon isotope discrimination and water use efficiency under regulated deficit irrigation of pear-iuiube tree显示文摘 | Ningbo Cui Taisheng Du Shaozhong Kang | 2009 | Agriculture Water Management2009，96，： | 1 |
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