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| 1 | Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize显示文摘In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome. | WANG FengGe ZHAO JiuRan DAI JingRui YI HongMei KUANG Meng SUN YanMei YU XinYan GUO JingLun WANG Lu | 2007 | Chinese Science Bulletin2007，52，2： | 35 |
| 2 | Chromosome-Level Reference Genome and Population Genomic Analysis Provide Insights into the Evolution and Improvement of Domesticated Mulberry (Morns alba)显示文摘Mulberry(Morns spp.)is the sole plant consumed by the domesticated silkworm.However,the genome of domesticated mulberry has not yet been sequenced,and the ploidy level of this species remains unclear.Here,we report a high-quality,chromosome-level domesticated mulberry(Morus alba)genome.Analysis of genomic data and karyotype analyses confirmed that M.alba is a diploid with 28 chromosomes(2/7=2x=28).Population genomic analysis based on resequencing of 134 mulberry accessions classified domesticated mulberry into three geographical groups,namely,Taihu Basin of southeastern China(Hu mulberry),northern and southwestern China,and Japan.Hu mulberry had the lowest nucleotide diversity among these accessions and demonstrated obvious signatures of selection associated with environmental adaptation.Further phylogenetic analysis supports a previous proposal that multiple domesticated mulberry accessions previously classified as different species actually belong to one species.This study expands our understanding of genome evolution of the genus Morus and population structure of domesticated mulberry,which would facilitate mulberry breeding and improvement. | Feng Jiao Rongsong Luo Xuelei Dai Hui Liu Gang Yu Shuhua Han Xin Lu Chao Su Qi Chen Qinxia Song Caiting Meng Fanghong Li Hongmei Sun Rui Zhang Tian Hui Yonghua Qian Aichun Zhao Yu Jiang | 2020 | Molecular Plant2020，13，7： | 10 |
| 3 | Effect of Cyclocarya paliurus polysaccharides on streptozotocin-induced diabetic nephropathy in rats显示文摘OBJEVTIVE:To investigate the efficacy of Cyclocarya paliurus(C.paliurus)polysaccharides on streptozotocin-induced diabetic nephropathy in rats.METHODS:Rats were divided into 6 groups,including group of normal control,group of diabetic control,group of metformin treatment,low-dose group of C.paliurus polysaccharides treatment,middle-dose group of C.paliurus polysaccharides treatment and high-dose group of C.paliurus polysaccharides treatment.Histological analysis of kidney was analyzed using hematoxilin and eosin.Levels of blood glucose,creatinine,urea,uric acid were determined by spectrophotometry.Anti-oxidative enzymes were measured by real-time polymerase chain reaction(PCR)and enzyme-linked immunosorbent assay(ELISA).Advanced glycation end products(AGEs)and transforming growth factor-β1(TGF-β1)level was measured by ELISA.RESULTS:Abnormal changes were observed in the group of diabetic control characterized by atrophy of the renal glomeruli with hypercellularity,congestion of glomerular tufts,dilation of the renal spaces,and degeneration of renal tubule.Compared with that of normal group,blood glucose,creatinine,urea,uric acid level was significantly increased in the group of diabetic control.Superoxide dismutase,catalase,glutathione peroxidase,glutathione reductase level was significantly decreased,but AGEs and TGF-β1 level was significantly increased.By contrast,administration of C.paliurus polysaccharides and metformin could reverse the above-mentioned results of the group of diabetic control,especially in the high-dose group of C.paliurus polysaccharides.CONCLUSION:Our findings suggest that C.paliurus polysaccharides may play a protecting role for nephropathy of diabetic rats by lowering glucose,creatinine,urea,uric acid level,enhancing the antioxidative ability,and reducing AGEs and TGF-β1 expression. | Xia Xichao Mao Dongxue Dai Hongmei Wu Xi Zhang Zuyuan Wang Huaying Zhou Wenwen Dong Yanmei Wang Mengqi Li Yuan Shao Xiangyang Ouyang Jingfeng | 2020 | Journal of Traditional Chinese Medicine2020，40，6： | 9 |
| 4 | The Leu477 and Leu613 of ORF2-Encoded Protein Are Critical in Forming Neutralization Antigenic Epitope of Hepatitis E Virus Genotype 4显示文摘Hepatitis E virus (HEV) genotype 4 was originally identified in China. Its neutralization antigenic epitopes have not been characterized. Recently, we identified a neutralizing monoclonal antibody (mAb) 1G10, which was generated following immunization of mice with p166Chn, a recombinant protein comprising 464-629 amino acids (aa) of the HEV genotype 4 capsid protein. In this study, a panel of 22 N- and/or C-terminal truncated and 6 site-directed mutated p166Chn proteins were prepared. Only those N- or C-terminal truncated proteins containing the region 477-613 aa could react with the mAb 1G10, suggesting the neutralization epitope of HEV genotype 4 is located between aa477 and aa613. However, a both N- and C-terminal truncated protein, pN477-C613, neither reacted to 1G10 nor elicited neutralizing antibodies in mice, while another both terminal truncated protein, pN472-C617, did, suggesting the flanking regions of the pN477-C613 could help to stabilize and allow presentation of the neutralization epitope to the immune system. Substituting Leu477 and/or Leu613 with the polar, uncharged threonine (Thr) caused ≥ 50% reduction of the mutants' immunoreactivity to 1G10, whereas replacement by hydrophobic phenylalanine (Phe) made little impact on the immunoreactivity, revealing functional associations between hydrophobicity of aa at positions 477 and 613 and the antigenicity of p166Chn. These data suggested Leu477 and Leu613 are critical in forming the neutralization epitope of HEV genotype 4. | Hongmei Zhang Xing Dai Xiangnian Shan Jsihong Meng | 2008 | Cellular & Molecular Immunology2008，5，6： | 9 |
| 5 | Response of water resources to climate change and its future trend in the source region of the Yangtze River显示文摘在这份报纸,在中国的表面水流动和它的气候的原因的变化用水文学被分析,气象学的观察数据,以及影响数据设定(版本 2.0 ) 在 2009 年 11 月由国家气候中心出版了。结果显示表面水资源在过去的 51 年在长江的来源区域显示出一个增加的趋势,特别在 2004 以后。趋势很清楚地被显示出,并且在加热地的西藏的高原提高了效果,和进入的高原季风的地方,有 9 年和 22 年的伪时期一个强壮的时期。显著地增加的降水,和冰河融化由于气候变化增加的水,所有哪个是为来源区域的水资源的增加的主要气候的原因。基于全球气候模型预言,在 SRESA1B 气候变化情形,水资源是可能的在这个区域增加下一 20 年。 | LlLin SHEN Hongyan DAI Sheng LI Hongmei XIAO Jianshe | 2013 | Journal of Geographical Sciences2013，23，2： | 8 |
| 6 | In uence of Particle Size on Laser Absorption and Scanning Track Formation Mechanisms of Pure Tungsten Powder During Selective Laser Melting显示文摘A three-dimensional laser absorption model based on ray tracing was established to describe the coupled interaction of a laser beam with particles in the powder layers of pure tungsten(W)material processed by selective laser melting(SLM).The influence of particle size on the powder-to-laser absorptivity and underlying absorption behavior was investigated.An intrinsic relationship between the absorption,distribution of absorbed irradiance within the powder layers,and surface morphology and geometric characteristics(e.g.,contact angle,width and height of tracks,and remelted depth)of the laser scanning tracks is presented here.Simulation conclusions indicate that the absorptivity of the powder layers considerably exceeds the single powder particle value or the dense solid material value.With an increase in particle size,the powder layer absorbs less laser energy.The maximum absorptivity of theWpowder layers reached 0.6030 at the particle size of 5 lm.The distribution of laser irradiance on the particle surface was sensitive to particle size,azimuthal angle,and the position of the powder particles on the substrate.The maximum irradiance in the powder layers decreased from 1.117×10^–3 to 0.85×10^–3W·μm^-2 and the contour of the irradiance distribution in the center of the irradiated area gradually contracted when the particle size increased from 5 to 45 lm.An experimental study on the surface morphologies and cross-sectional geometric characteristics of SLM-fabricated W material was performed,and the experimental results validated the mechanisms of the powder-to-laser-absorption behavior that were obtained in simulations.This work provides a scientific basis for the application of the ray-tracing model to predict the wetting and spreading ability of melted tracks during SLM additive manufacturing in order to yield a sound laser processability. | Jiayao Zhang Dongdong Gu Ying Yang Hongmei Zhang Hongyu Chen Donghuai Dai Kaijie Lin | 2019 | Engineering2019，5，4： | 6 |
| 7 | A male-ABCD algorithm for hepatocellular carcinoma risk prediction in HBs Ag carriers显示文摘Objective: Hepatocellular carcinoma(HCC) development among hepatitis B surface antigen(HBs Ag) carriers shows gender disparity, influenced by underlying liver diseases that display variations in laboratory tests. We aimed to construct a risk-stratified HCC prediction model for HBs Ag-positive male adults.Methods: HBs Ag-positive males of 35-69 years old(N=6,153) were included from a multi-center populationbased liver cancer screening study. Randomly, three centers were set as training, the other three centers as validation. Within 2 years since initiation, we administrated at least two rounds of HCC screening using Bultrasonography and α-fetoprotein(AFP). We used logistic regression models to determine potential risk factors,built and examined the operating characteristics of a point-based algorithm for HCC risk prediction.Results: With 2 years of follow-up, 302 HCC cases were diagnosed. A male-ABCD algorithm was constructed including participant's age, blood levels of GGT(γ-glutamyl-transpeptidase), counts of platelets, white cells,concentration of DCP(des-γ-carboxy-prothrombin) and AFP, with scores ranging from 0 to 18.3. The area under receiver operating characteristic was 0.91(0.90-0.93), larger than existing models. At 1.5 points of risk score,26.10% of the participants in training cohort and 14.94% in validation cohort were recognized at low risk, with sensitivity of identifying HCC remained 100%. At 2.5 points, 46.51% of the participants in training cohort and 33.68% in validation cohort were recognized at low risk with 99.06% and 97.78% of sensitivity, respectively. At 4.5 points, only 20.86% of participants in training cohort and 23.73% in validation cohort were recognized at high risk,with positive prediction value of 22.85% and 12.35%, respectively.Conclusions: Male-ABCD algorithm identified individual's risk for HCC occurrence within short term for their HCC precision surveillance. | Yuting Wang Minjie Wang He Li Kun Chen Hongmei Zeng Xinyu Bi Zheng Zhu Yuchen Jiao Yong Wang Jian Zhu Hui Zhao Xiang Liu Chunyun Dai Chunsun Fan Can Zhao Deyin Guo Hong Zhao Jianguo Zhou Dongmei Wang Zhiyuan Wu Xinming Zhao Wei Cui Xuehong Zhang Jianqiang Cai Wanqing Chen Chunfeng Qu | 2021 | Chinese Journal of Cancer Research2021，33，3： | 3 |
| 8 | Profiles and Source Apportionment of Nonmethane Volatile Organic Compounds in Winter and Summer in Xi’an, China, based on the Hybrid Environmental Receptor Model显示文摘Summer and winter campaigns for the chemical compositions and sources of nonmethane hydrocarbons(NMHCs)and oxygenated volatile organic compounds(OVOCs)were conducted in Xi’an.Data from 57 photochemical assessment monitoring stations for NMHCs and 20 OVOC species were analyzed.Significant seasonal differences were noted for total VOC(TVOC,NMHCs and OVOCs)concentrations and compositions.The campaign-average TVOC concentrations in winter(85.3±60.6 ppbv)were almost twice those in summer(47.2±31.6 ppbv).Alkanes and OVOCs were the most abundant category in winter and summer,respectively.NMHCs,but not OVOCs,had significantly higher levels on weekends than on weekdays.Total ozone formation potential was higher in summer than in winter(by 50%)because of the high concentrations of alkenes(particularly isoprene),high temperature,and high solar radiation levels in summer.The Hybrid Environmental Receptor Model(HERM)was used to conduct source apportionment for atmospheric TVOCs in winter and summer,with excellent accuracy.HERM demonstrated its suitability in a situation where only partial source profile data were available.The HERM results indicated significantly different seasonal source contributions to TVOCs in Xi’an.In particular,coal and biomass burning had contributions greater than half in winter(53.4%),whereas traffic sources were prevalent in summer(53.1%).This study’s results highlight the need for targeted and adjustable VOC control measures that account for seasonal differences in Xi’an;such measures should target not only the severe problem with VOC pollution but also the problem of consequent secondary pollution(e.g.,from ozone and secondary organic aerosols). | Jian SUN Zhenxing SHEN Yue ZHANG Wenting DAI Kun HE Hongmei XU Zhou ZHANG Long CUI Xuxiang LI Yu HUANG Junji CAO | 2021 | Advances in Atmospheric Sciences2021，38，1： | 2 |
| 9 | Gene expression profiling under different photoperiod/temperature conditions in a photoperiod-/thermo-sensitive genic male sterile line of rice(Oryza sativa L.)显示文摘Peiai 64S is a photoperiod- and thermo- sensitive genic male sterile (PTSGMS) line of rice, which is male sterile at long day/high temperature and partial fertile at short day/low temperature. A cDNA array representing 3328 unique rice genes was used to profile the gene expression patterns in the young panicles of Peiai 64S under these two condi- tions. The statistical data showed that up to 14.60% of genes exhibited up-or down-regulated expressions in the plants at long day/high temperature compared with plants at short day/low temperature. Only four genes were up-regulated while 482 genes down-regulated. Real-time PCR with all the up-regulated and 9 randomly selected down-regulated genes confirmed the differential expressions detected by the array, indicating that the constructed cDNA array is reliable. These differently expressed genes participated in almost all cell biological responses. Analysis of up- and down-regulated genes revealed distinctive changes between the mRNA abundances of MMK1 and MMK2, both of which are analogs ofMAPK, and significant down-regulation of several transcription factors. It was suggested that changes that occurred in the MAPK signal transduction path- way might disturb the transcription control necessary for morphogenesis of pollens and corresponding phys- iological functions. | GUO Xiaoqin DONG Haitao ZHENG Kangle LUO Hongmei TAN Xuelin FANG Yongqi WANG Yanqin DENG Ye DAI Chengen LOU Yichun SHAO Jing SHI Wenqin ZHAO Dong LI Debao | 2006 | Chinese Science Bulletin2006，51，2： | 2 |
| 10 | Comparison of dimension reduction-based logistic regression models for case-control genome-wide association study:principal components analysis vs.partial least squares显示文摘With recent advances in biotechnology, genome-wide association study(GWAS) has been widely used to identify genetic variants that underlie human complex diseases and traits. In case-control GWAS, typical statistical strategy is traditional logistical regression(LR) based on single-locus analysis. However, such a single-locus analysis leads to the well-known multiplicity problem, with a risk of inflating type I error and reducing power. Dimension reduction-based techniques, such as principal component-based logistic regression(PC-LR), partial least squares-based logistic regression(PLS-LR), have recently gained much attention in the analysis of high dimensional genomic data. However, the perfor?mance of these methods is still not clear, especially in GWAS. We conducted simulations and real data application to compare the type I error and power of PC-LR, PLS-LR and LR applicable to GWAS within a defined single nucleotide polymorphism(SNP) set region. We found that PC-LR and PLS can reasonably control type I error under null hypothesis.On contrast, LR, which is corrected by Bonferroni method, was more conserved in all simulation settings. In particular, we found that PC-LR and PLS-LR had comparable power and they both outperformed LR, especially when the causal SNP was in high linkage disequilibrium with genotyped ones and with a small effective size in simulation. Based on SNP set analysis, we applied all three methods to analyze non-small cell lung cancer GWAS data. | Honggang Yi Hongmei Wo Yang Zhao Ruyang Zhang Junchen Dai Guangfu Jin Hongxia Ma Tangchun Wu Zhibin Hu Dongxin Lin Hongbing Shen Feng Chen | 2015 | The Journal of Biomedical Research2015，29，4： | 2 |
| 11 | Responses of East Asian summer monsoon to historical SST and atmospheric forcing during 1950–2000显示文摘 | Hongmei Li Aiguo Dai Tianjun Zhou Jian Lu | | Climate Dynamics0，，： | 2 |
| 12 | Neo- natal chlorpyrifos exposure induces loss of dopaminergie neu- rons in young adult rats 显示文摘 | ZHANG Jle DAI Hongmei DENG Yuanying | 2015 | Toxicology2015，336，： | 1 |
| 13 | An Improved Stator Flux Observation Method of Permanent Magnet Synchronous Motor显示文摘The stator flux and electromagnetic torque observation is the basis of direct torque controlled permanent magnet synchronous motor( PMSM) drive system. However,the traditional stator flux observer based on voltage model is affected by integral initial values and integral drift,that based on current model is affected by the parameters of PMSM,so a new stator flux observation method is proposed based on an improved secondorder generalized integrator( SOGI). Compared to the stator flux observation method based on the conventional SOGI,the proposed method can not only overcome the influence of integral initial values and integral drift,but also completely eliminate the DC offset's influence. Therefore,the observation accuracy of stator flux is further improved. The simulation and experimental results both show that the proposed method has a higher stator flux and electromagnetic torque observation precision. | Guangjing Su Hongmei Li Ying Dai Zheng Li | 2016 | Journal of Harbin Institute of Technology(New Series)2016，23，6： | 1 |
| 14 | Photosensitivity in Ce/Sm-decorated Keggin polyoxomolybdates:an interesting photoinduced coloration phenomenon of mixed- metal clusters involving energy transfer from rare-earth fluorescence显示文摘Ce/Sm ions are covalently grafted to a Keggin polyoxomolybdate cluster[PMo12O40]3-to yield interesting fluorescent-photodarkening molecular systems.Their photoresponce processes are observed to be activated via energy transfer from luminescent fragments to photocoloration components. | LIU Li LI HongMei DAI JingCao | 2014 | Science China Chemistry2014，57，6： | 1 |
| 15 | High-fidelity multichannel audio coding with Karhunen-Loevetransform显示文摘 | DAI Y HONGMEI A KYRIAKAKIS C | 2003 | IEEE Transactions on Speech and Audio Processing2003，11，4： | 1 |
| 16 | Clinical Study on the Anti-Aging Effect of a Cream Containing Ginseng Root Extract and L-Carnosine显示文摘The anti-aging effects of a cream containing L-carnosine and ginseng root extract to provide a reference for the evaluation and development of anti-aging cosmetics were tested. Sixty healthy females, aged 35-60,were grouped into test sample and placebo groups. Skin elasticity, gloss, and wrinkles of the participants were analyzed before use and after use for 8 weeks. Instrument measurement results showed that after 8 weeks,skin elasticity, gloss, and wrinkles in the members of the test sample group obviously improved relative to those of the placebo group. Clinical effect assessment results showed that subjects did not experience adverse skin reactions, and their skin gloss showed obvious improvement. Self-assessment results showed that 87%subjects are satisfied with the test sample. Cream containing L-carnosine and ginseng root extract has a certain anti-aging effect. | Sun Hongmei Dai Jieling Yang Qiongli Li Yingyi Liu Fengsong | 2018 | China Detergent & Cosmetics2018，3，3： | 1 |
| 17 | In-plane epitaxial growth of 2D CoSe-WSe2 metalsemiconductor lateral heterostructures with improved WSe2 transistors performance显示文摘The two-dimensional(2D)in-plane(lateral)heterostructures have attracted increasing interest for potential applications in the atomically thin electronics and optoelectronics.While most studies focus on semiconductorsemiconductor lateral heterostructures with highly similar lattice structures between the constituent components,the synthesis of metal-semiconductor lateral heterostructures is much less explored and usually more challenging due to more distinct lattice structures or chemical properties.Herein,a vapor phase epitaxy growth method of high-quality metal-semiconductor lateral heterostructures between tetragonal CoSe and hexagonal WSe2 is reported.The 2D CoSe can selectively nucleate at the edge of pre-grown WSe2 nanosheets to form CoSe-WSe2 metal-semiconductor lateral heterostructures.Optical microscopy(OM),scanning electron microscopy(SEM),and atomic force microscopy(AFM)studies reveal distinct contrast across the heterostructure interface.High-resolution transmission electron microscopy(HRTEM)and selected area electron diffraction(SAED)studies further confirm the microstructure modulation across the heterostructure interface.The field-effect transistors(FETs)of CoSe-WSe2 lateral heterostructures show satisfactory Ohmic contacts and considerably better FET performance over those with deposited Cr/Au contacts,suggesting the in-plane metal-semiconductor junctions may function as improved contacts for the atomically thin electronics. | Huifang Ma Kejing Huang Ruixia Wu Zhengwei Zhang Jia Li Bei Zhao Chen Dai Ziwei Huang Hongmei Zhang Xiangdong Yang BoLi Yuan Liu Xiangfeng Duan Xidong Duan | 2021 | InfoMat2021，3，2： | 1 |
| 18 | High-Fidelity Multichannel Audio Coding with Karhunen-Loeve Transform显示文摘 | YANG Dai A! Hongmei KYRIAKAKIS C | 2003 | IEEE Transac- tions on Speech and Audio Processing2003，11，4： | 1 |
| 19 | Global Identification of Significantly Expressed Genes in Developing Endosperm of Rice by Expression Sequence Tags and cDNA Array Approaches显示文摘Rice endosperm plays a very important role in seedling germination and determines the qualities of rice grain. Although studies on specific gene categories in endosperm have been carried out, global view of gene expression at a transcription level in rice endosperm is still limited. To gain a better understanding of the global and tissue-specific gene expression profiles in rice endosperm, a cDNA library from rice endosperm of immature seeds was sequenced. A cDNA array was constructed based on the tentative unique transcripts derived from expression sequence tag (EST) assembling results and then hybridized with cDNAs from five different tissues or organs including endosperm, embryo, leaf, stem and root of rice. Significant redundancy was found for genes encoding prolamin, glutelin, allergen, and starch synthesis proteins, accounting for ~34% of the total ESTs obtained. The cDNA array revealed 87 significantly expressed genes in endosperm compared with the other four organs or tissues. These genes included 13 prolamin family proteins, 17 glutelin family proteins, 12 binding proteins, nine catalytic proteins and four ribosomal proteins, indicating a complicated biological processing in rice endosperm. In addition, Northern verification of 1,4-alpha-glucan branching enzyme detected two isoforms in rice endosperm, the larger one of which only existed in endosperm. | Qichao Tu Haitao Dong Haigen Yao Yongqi Fang Cheng'en Dai Hongmei Luo Jian Yao Dong Zhao Debao Li | 2008 | Journal of Integrative Plant Biology2008，50，9： | 0 |
| 20 | Tribological behaviors of graphene oxide partly substituted with nano-SiO_(2) as lubricant additives in water for magnesium alloy/steel interfaces显示文摘Although graphene oxide(GO)has emerged as an excellent lubricant additive in water,there remain great challenges in their practical application due to high production costs.By taking into account the low cost and also its excellent tribological properties,it is likely that nano-SiO_(2)can be used as a lubricant additive to partially replace GO.Hence,this paper aims to explore the tribological properties of nano-SiO_(2)incorporated in GO nanofluids for partial GO replacement by investigating the friction coefficient and wear volume of the prepared SiO_(2)/GO hybrid nanofluids for magnesium alloy/steel sliding pairs.The experiments reveal that the SiO_(2)/GO hybrids retain low friction coefficients as compared to individual GO or SiO_(2)at all test conditions in this study.However,as for the bearing capacity test,all samples can provide a low wear volume under the loads of 1 and 3 N.With the increase of the normal load,there is considerable differences in the anti-wear behavior.Compared with that of individual GO nanofluids,the wear volume of the GO/SiO_(2)(mass ratio of 0.3:0.2)hybrid nanofluids was reduced by50.5%at 5 N and by 49.2%at 8 N.Furthermore,the wear volume of the GO/SiO_(2)(mass ratio of 0.3:0.2)hybrid nanofluids was reduced by46.3%under the rigorous conditions,as compared to individual GO nanofluids.The findings provide new insights into developing carbon nanomaterial-based hybrid nanofluids for magnesium alloy formation. | Hongmei Xie Jiahong Dai Dan Zhou | 2022 | International Journal of Minerals,Metallurgy and Materials2022，29，7： | 0 |
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