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| 1 | Prevalence of sleep-disordered breathing in a 316-patient French cohort of stable congestive heart failure显示文摘 | Paulino A Damy T Margarit L | 2009 | Arch Cardiovasc Dis2009，102，3： | 1 |
| 2 | Buccinator Myomucosal Flap as Soft Tissue Covering for Vascularized Free Fibular Flap in Anterior Maxillary Bony Defects显示文摘 | Raúl González-García Luis Ruiz-Laza Damián Manzano Leticia Román-Romero Carlos Moreno Florencio Monje | 2010 | Journal of Oral and Maxillofacial Surgery2010，，4： | 1 |
| 3 | Drugs of abuse and their metabolites in the Ebro River basin: Occurrence in sewage and surface water, sewage treatment plants removal efficiency, and collective drug usage estimation显示文摘 | Cristina Postigo María José López de Alda Damià Barceló | 2009 | Environment International2009，，1： | 1 |
| 4 | Mesenchymal stem cells: biological properties and clinical applications显示文摘 | Ignacio García-Gómez Gema Elvira Agustín G Zapata María L Lamana Manuel Ramírez Javier García Castro Mariano García Arranz Angeles Vicente Juan Bueren Damián García-Olmo | 2010 | Expert Opinion on Biological Therapy2010，，10： | 1 |
| 5 | Prevalence of sleep-disordered breathing in a 316-patient French cohort of stable congestive heart failure显示文摘 | Paulino A Damy T Margarit L | 2009 | Arch Cardiovasc Dis2009，102，3： | 1 |
| 6 | Power-law distribution of the world wide web显示文摘 | DAMIE L A A HUBERMAN B A | 2000 | Science2000，287，5461： | 1 |
| 7 | Prevalence of sleep-disorder- ed breathing in a 316-patient French cohort of stable congestive heart failure显示文摘 | Panlino A Damy T Margarit L | 2009 | Archi Cardiovasc Dis2009，102，3： | 1 |
| 8 | Prognostic impact of sleep-dis- ordered breathing and its treatment with nocturnal ventilation for chronic heart failure显示文摘 | Damy T Margarit L Norec A | 2009 | Eur J Heart Failure2009，14，9： | 1 |
| 9 | Detection of KRAS G12D in colorectal cancer stool by droplet digital PCR显示文摘AIM To assess KRAS G12 D mutation detection by droplet digital PCR(dd PCR) in stool-derived DNA from colorectal cancer(CRC) patients.METHODS In this study, tumor tissue and stool samples were collected from 70 patients with stage Ⅰ-Ⅳ CRC diagnosed by preoperative biopsy. KRAS mutational status was determined by pyrosequencing analysis of DNA obtained from formalin-fixed paraffinembedded(FFPE) tumor tissues. The KRAS G12 D mutation was then analyzed by dd PCR in FFPE tumors and stool-derived DNA from patients with this point mutation. Wild-type(WT) tumors, as determined by pyrosequencing, were included as controls; analysis of FFPE tissue and stool-derived DNA by dd PCR was performed for these patients as well.RESULTS Among the total 70 patients included, KRAS mutations were detected by pyrosequencing in 32(45.71%), whereas 38(54.29%) had WT tumors. The frequency of KRAS mutations was higher in left-sided tumors(11 located in the right colon, 15 in the left, and 6 in the rectum). The predominant point mutation was KRAS G12 D(14.29%, n = 10), which was more frequent in early-stage tumors(I-IIA, n = 7). In agreement with pyrosequencing results, the KRAS G12 D mutation was detected by dd PCR in FFPE tumor-derived DNA, and only a residual number of mutated copies was found in WT controls. The KRAS G12 D mutation was also detected in stool-derived DNA in 80% of all fecal samples from CRC patients with this point mutation. CONCLUSION dd PCR is a reliable and sensitive method to analyze KRAS G12 D mutation in stool-derived DNA from CRC patients, especially at early stages. This non-invasive approach is potentially applicable to other relevant biomarkers for CRC management. | Susana Olmedillas-López Dennis César Lévano-Linares Carmen Laura Aúz Alexandre Luz Vega-Clemente Edurne León Sánchez Alejandro Villagrasa Jaime Ruíz-Tovar Mariano García-Arranz Damián García-Olmo | 2017 | World Journal of Gastroenterology2017，23，39： | 0 |
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