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| 1 | A High-Density SNP Genotyping Array for Rice Biology and Molecular Breeding显示文摘一个高密度的单个核苷酸多型性(SNP ) 数组为遗传学者和分子的 breeders.With 是极其重要的 genomic 的巨大的数量的累积重新定序为精确 SNP 察觉的数据和可得到的技术,设计高密度、高质量的米饭 SNP 数组是可能的。这里,我们报导一个高密度的 riceSNP 数组和它的实用程序的开发。SNP 探针被屏蔽超过 10 设计从变化和一个数组说出 RiceSNP50 的 801 米饭的 re-sequencingdata 提取的 000 000 SNP loci 在 Illumina Infinium 站台上被生产。数组 contained51 478 个均匀地分布式的标记,其 68% 个在遗传因子的区域以内。有 parent/F1 relationshipswere 的几百米饭植物过去常为精确 SNP 打电话产生一个高质量的簇文件。应用程序测试证明这穿有的 highgenotyping 精确性,并且能被用于不同目的。例如,有好分辨率的精英米饭变化 wasclustered 的一个核心集合。染色体宽的协会研究(GWAS ) 分析正确地识别了描绘的 QTL.Further,这个数组成功地为变化确认和特点基因渗入被使用。作为一个精确 high-throughputgenotyping 工具, RiceSNP50 将在两功能的 genomics 学习和分子的 breeding.Key 词起一个重要作用: | Haodong Chen Weibo Xie Hang He Huihui Yu Wei Chen Jing Li Renbo Yu Yue Yao Wenhui Zhang Yuqing He Xiaoyan Tang Fasong Zhou Xing Wang Deng Qifa Zhang | 2014 | Molecular Plant2014，7，3： | 38 |
| 2 | Origins and evolution of plant diversity in the Hengduan Mountains, China显示文摘1.Introduction The Hengduan Mountains region(HDM)in southwest China,one of the earth's 34 biodiversity hotspots,is characterized by its unique geology,dramatic topography,a climate where snow and below freezing temperatures can occur on any day of the year,by its location at elevations averaging between(1400e)2000 and4500(e5300)meters above sea level(m a.s.l.),and by one of | Hang Sun Jianwen Zhang Tao Deng David E. Boufford | 2017 | Plant Diversity2017，39，4： | 28 |
| 3 | A Genome-Wide Transcription Analysis Reveals a Close Correlation of Promoter INDEL Polymorphism and Heterotic Gene Expression in Rice Hybrids显示文摘杂种优势,或混合活力,指二个生来的变化的混合子孙在展出提高的生长或农学的性能的现象。尽管研究的世纪长的历史关于杂种优势的基因基础产生了几个假设,位于杂种优势和 heterotic 基因表示下面的分子的机制留下逃犯。这里,我们在米饭报导二个 heterotic 十字的染色体宽的基因表示分析,利用它的充分定序的染色体。约 79% 基因是从 heterotic 十字的二个集合在幼苗射击表示的差别,包括许多抄写因素基因,并且展出了基因行动的多重模式。在生来的父母之间的 ortholog 基因的通常认为的倡导者区域的比较揭示了广泛的顺序变化,特别地小的插入 / 删除(INDEL ) ,许多哪个在通常认为的 cis 规章的元素的形成 / 混乱的结果。一起,这些结果建议那在抄写因素和多态的倡导者的表示之间的组合相互影响在混血儿的 cis 规章的元素是位于 heterotic 基因行动下面的一嘴巧的分子的机制并且这样在米饭的杂种优势。 | Hui-Yong Zhang Hang He Liang-Bi Chen Lei Li Man-Zhong Liang Xiang-Feng Wang Xi-Gang Liu Guang-Ming He Run-Sheng Chen Li-Geng Ma Xing Wang Deng | 2008 | Molecular Plant2008，1，5： | 25 |
| 4 | Review on the progress of ultra-precision machining technologies显示文摘 | Julong YUAN Binghai LYU Wei HANG Qianfa DENG | 2017 | Frontiers of Mechanical Engineering2017，12，2： | 15 |
| 5 | Hyperparameter Optimization for Machine Learning Models Based on Bayesian Optimization显示文摘Hyperparameters are important for machine learning algorithms since they directly control the behaviors of training algorithms and have a significant effect on the performance of machine learning models. Several techniques have been developed and successfully applied for certain application domains. However, this work demands professional knowledge and expert experience. And sometimes it has to resort to the brute-force search.Therefore, if an efficient hyperparameter optimization algorithm can be developed to optimize any given machine learning method, it will greatly improve the efficiency of machine learning. In this paper, we consider building the relationship between the performance of the machine learning models and their hyperparameters by Gaussian processes. In this way, the hyperparameter tuning problem can be abstracted as an optimization problem and Bayesian optimization is used to solve the problem. Bayesian optimization is based on the Bayesian theorem. It sets a prior over the optimization function and gathers the information from the previous sample to update the posterior of the optimization function. A utility function selects the next sample point to maximize the optimization function.Several experiments were conducted on standard test datasets. Experiment results show that the proposed method can find the best hyperparameters for the widely used machine learning models, such as the random forest algorithm and the neural networks, even multi-grained cascade forest under the consideration of time cost. | Jia Wu Xiu-Yun Chen Hao Zhang Li-Dong Xiong Hang Lei Si-Hao Deng | 2019 | Journal of Electronic Science and Technology2019，17，1： | 14 |
| 6 | Efficient generation of pink-fruited tomatoes using CRISPR/Cas9 system显示文摘Tomato(Solanum lycopersicum)is the leading vegetable crop worldwide and an essential component of a healthy diet(Lin et al.,2014;Du et al.,2017).Fruit color is regarded as one of the most important commercial traits in tomato(The Tomato Genome Consortium,2012).Consumers in different regions have different color preferences.For example,European and | Lei Deng Hang Wang Chuanlong Sun Qian Li Hongling Jiang Minmin Du Chang-Bao Li Chuanyou Li | 2018 | Journal of Genetics and Genomics2018，45，1： | 13 |
| 7 | Is the East Asian flora ancient or not?显示文摘The East Asian flora(EAF) is a key biodiversity hotspot for understanding the origin and evolution of Northern Hemisphere floras, but there is an ongoing debate on whether it is a museum or a cradle for seed plants. Within the EAF, two main floras, the Sino-Himalayan Flora(mainly the Rhododendron Flora) and the Sino-Japanese Flora(mainly the Metasequoia Flora), have been recognized. Previous studies suggested that the EAF is ancient and the Metasequoia Flora is older than the Rhododendron Flora. To test this hypothesis, we synthesized molecular as well as fossil data on seed plants, focusing on the biogeographical origins and historical evolution of the EAF. We compared the ages of its two constituent floras, and examined the impact of the Asian monsoon and other environmental changes on the development of EAF through meta-analysis. Our results suggest that the EAF might be relatively young, with most of its clades originating since the Miocene. The Rhododendron Flora and the Metasequoia Flora are probably of a similar age. The formation and development of the Asian monsoon might have been the main factors that have driven the evolution of EAF. In the Rhododendron Flora, the north-south mountain chains increased the concentration of species and reduced extinction, and the barriers between the east and west have resulted in species differentiation, which triggered it to become a diversity center. The EAF appears to have multiple biogeographical origins, having closely affiliated not only with other floras in the Northern Hemisphere, but also with Gondwanan floras. | Yong-Sheng Chen Tao Deng Zhuo Zhou Hang Sun | 2018 | National Science Review2018，5，6： | 13 |
| 8 | Integrative genome-wide analysis reveals HLP1, a novel RNA-binding protein, regulates plant flowering by targeting alternative polyadenylation显示文摘 | Yong Zhang Lianfeng Gu Yifeng Hou Lulu Wang Xian Deng Runlai Hang Dong Chen Xiansheng Zhang Yi Zhang Chunyan Liu Xiaofeng Cao | 2015 | Cell Research2015，25，7： | 11 |
| 9 | A Global Identification and Analysis of Small Nucleolar RNAs and Possible Intermediate-Sized Non-Coding RNAs in Oryza sativa显示文摘积累的证据建议非编码的 RNA (ncRNAs ) 在许多真核细胞的有机体普遍、机能上地重要。在这研究,我们采用了分别和 cDNA 图书馆构造方法列在后面由的一种特殊尺寸 454 系统地介绍米饭的深定序中间尺寸的 ncRNAs。我们的分析总共导致了 1349 ncRNAs 的鉴定,包括一个未知功能的范畴的 754 新奇 ncRNAs。所有识别 ncRNAs 的染色体分发没显示出海滨偏爱,并且显示了类似于与很少染色体相关性在编码蛋白质的基因观察了那的一个模式。非常, ncRNAs 的一半被集中在附近编码区域的 5 和 3 个终点正海滨。新奇 ncRNAs 的多数是米饭特定,当 78% 小核仁的 RNA (snoRNAs ) 被保存时。双人脚踏车复制驾驶了扩大在 snoRNA 基因家庭的一半上。而且, 90% snoRNA 候选人被显示特别地生产在 2030 nt,其 80% 通常与淘金者蛋白质被联系,和 AGO1b 之间的小 RNA。总的来说,我们的调查结果在 monocot 种提供中间尺寸的非编码的 transcriptome 的一个全面看法,它将为 ncRNA 函数的深入的分析用作一个有用平台。 | Ting-Ting Liu Danmeng Zhu Wei Chen Wei Deng Hang He Guangming He Baoyan Bai Yijun Qi Runsheng Chen Xing Wang Deng | 2013 | Molecular Plant2013，6，3： | 10 |
| 10 | Expression Analysis of miRNAs and Highly-expressed Small RNAs in Two Rice Subspecies and their Reciprocal Hybrids显示文摘Heterosis,or hybrid vigor,is the phenomenon whereby progeny of two inbred lines exhibit superior agronomic performance compared with either parent.We analyzed the expression of miRNAs and highly expressed small RNAs (defined according to Solexa sequencing results) in two rice (Oryza sativa) subspecies (japonica cv.Nipponbare and indica cv.93-11) and their reciprocal hybrids using microarrays.We found that of all the 1141 small RNAs tested,140 (12%,140 of 1141) and 157 (13%,157 of 1141) were identified being significantly differentially expressed in two reciprocal hybrids,respectively.All possible modes of action,including additive,high-and low-parent,above high-and below low-parent modes were exhibited.Both F1 hybrids showed non-additive expression patterns,with downregulation predominating.Interestingly,15 miRNAs displayed stark opposite expression trends relative to midparent in reciprocal hybrids.Computational prediction of targets of differentially expressed miRNAs showed that they participated in multifaceted developmental pathways,and were not distinguishable from the targets of non-differentially expressed miRNAs.Together,our findings reveal that small RNAs play roles in heterosis and add a new layer in the understanding and exploitation of molecular mechanisms of heterosis. | Fangfang Chen Guangming He Hang He Wei Chen Xiaopeng Zhu Manzhong Liang Liangbi Chen Xing Wang Deng | 2010 | Journal of Integrative Plant Biology2010，52，11： | 10 |
| 11 | Origin and Evolution of Core Components Responsible for Monitoring Light Environment Changes during Plant Terrestrialization显示文摘Light serves as the source of energy as well as an information signal for photosynthetic plants. During evolution, plants have acquired the ability to monitor environmental light radiation and adjust their developmental patterns to optimally utilize light energy for photosynthesis. The mechanisms of light perception and signal transduction have been comprehensively studied in past decades, mostly in a few model plants, including Arabidopsis thaliana. However, systematic analyses of the origin and evolution of core components involved in light perception and signaling are still lacking. In this study, we took advantage of the recently sequenced genomes and transcriptomes covering all the main Archaeplastida clades in the public domain to identify orthologous genes of core components involved in light perception and signaling and to reconstruct their evolutionary history. Our analyses suggested that acclimation to different distribution of light quality in new environments led to the origination of specific light signaling pathways in plants. The UVR8 (UV Resistance Locus 8) signaling pathway originated during the movement of plants from the deeper sea to shallow water and enabled plants to deal with ultraviolet B light (UV-B). After acquisition of UV-B adaptation, origination of the phytochrome signaling pathway helped plants to colonize water surface where red light became the prominent light energy source. The seedling emergence pathway, which is mediated by a combination of light and phytohormone signals that orchestrate plant growth pattern transitions, originated before the emergence of seed plants. Although cryptochromes and some key components of E3 ubiquitin ligase systems already existed before the divergence of the plant and animal kingdoms, the coevolution and optimization of light perception and downstream signal transduction components, including key transcription factors and E3 ubiquitin ligase systems, are evident during plant terrestrialization. | Xue Han Xin Chang Zhenhua Zhang Haodong Chen Hang He Bojian Zhong Xing Wang Deng | 2019 | Molecular Plant2019，12，6： | 9 |
| 12 | Magnesium-based biomaterials as emerging agents for bone repair and regeneration:from mechanism to application显示文摘Magnesium(Mg)is the fourth most abundant element in the human body and is important in terms of specific osteogenesis functions.Here,we provide a comprehensive review of the use of magnesium-based biomaterials(MBs)in bone reconstruction.We review the history of MBs and their excellent biocompatibility,biodegradability and osteopromotive properties,highlighting them as candidates for a new generation of biodegradable orthopedic implants.In particular,the results reported in the field-specific literature(280 articles)in recent decades are dissected with respect to the extensive variety of MBs for orthopedic applications,including Mg/Mg alloys,bioglasses,bioceramics,and polymer materials.We also summarize the osteogenic mechanism of MBs,including a detailed section on the physiological process,namely,the enhanced osteogenesis,promotion of osteoblast adhesion and motility,immunomodulation,and enhanced angiogenesis.Moreover,the merits and limitations of current bone grafts and substitutes are compared.The objective of this review is to reveal the strong potential of MBs for their use as agents in bone repair and regeneration and to highlight issues that impede their clinical translation.Finally,the development and challenges of MBs for transplanted orthopedic materials are discussed. | Hang Zhou Bing Liang Haitao Jiang Zhongliang Deng Kexiao Yu | 2021 | Journal of Magnesium and Alloys2021，9，3： | 9 |
| 13 | A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding显示文摘Watermelon,Citrullus lanatus,is the world's third largest fruit crop.Reference genomes with gaps and a narrow genetic base hinder functional genomics and genetic improvement of watermelon.Here,we report the assembly of a telomere-to-telomere gap-free genome of the elite watermelon inbred line G42 by incorporating high-coverage and accurate long-read sequencing data with multiple assembly strategies.All 11 chromosomes have been assembled into single-contig pseudomolecules without gaps,representing the highest completeness and assembly quality to date.The G42 reference genome is 369321829 bp in length and contains 24205 predicted protein-coding genes,with all 22 telomeres and 11 centromeres characterized.Furthermore,we established a pollen-EMS mutagenesis protocol and obtained over 200000M1 seeds from G42.In a sampling pool,48 monogenic phenotypic mutations,selected from 223M1and 78 M2 mutants with morphological changes,were confirmed.The average mutation density was 1 SNP/1.69Mband1 indel/4.55 Mb per M1 plant and 1SNP/1.08Mb and 1 indel/6.25 Mb per M2 plant.Taking advantage of the gap-free G42 genome,8039 mutations from 32 plants sampled from M1 and M2 families were identified with 100%accuracy,whereas only 25% of the randomly selected mutations identified using the 97103v2 reference genome could be confirmed.Using this library and the gap-free genome,two genes responsible for elongated fruit shape and male sterility(CiMs1)were identified,both caused by a single basechange from G to A.The validated gap-free genome and its EMS mutation library provide invaluable resources for functional genomics and genetic improvement of watermelon. | Yun Deng Shoucheng Liu Yilin Zhang Jingsheng Tan Xiaopeng Li Xiao Chu Binghua Xu Yao Tian Yudong Sun Bosheng Li YunbiXu Xing Wang Deng Hang He Xingping Zhang | 2022 | Molecular Plant2022，15，8： | 9 |
| 14 | Plastome phylogenomic analysis of Torreya (Taxaceae)显示文摘Torreya Arn,a small genus of Taxaceae,consists of six species occurring in North America and eastern Asia.Several phylogenetic studies have previously been undertaken to reveal relationships within this genus,although only a few DNA segments or species were used.In the present study,we sequenced five Torreya plastomes and combined these with two existing plastomes from the genus to investigate plastome evolution and phylogenetic relationships within Torreya.All sequenced Torreya plastomes shared the same complement of 82 protein-coding genes,4 ribosomal RNA genes,and 31 transfer RNA genes.Phylogenetic inference using a maximum likelihood framework consisted df an 82-gene,17-taxon dataset,including all species of Torreya,resolved Torreya as a monophyletic clade.Strongly supported relationships within the genus include the position of the early diverging T.jackii Chun,the two sister pairs T.fargesii Franch.-T.nucifera(L.)Siebold&Zucc.and T.grandis Fortune ex Lindl.-T.californica Torr,and the monophyly of the clade induding T.fargesii var.yunnanensis,T.fargesi,and T.nucifera.In addition to the inference of species relationships,divergence time estimation and biogeographical analysis were carried out.The diversification of Torreya was estimated to be approximately 8.9 Ma.Ancestral state reconstruction of the geographical area suggested China/eastern North America as the most likely ancestral region for the six extant Torreya species. | Xu Zhang Hua-Jie Zhang Jacob BLandis Tao Deng Ai-Ping Meng Hang Sun Yan-Song Peng Heng-Chang Wang Yan-Xia Sun | 2019 | Journal of Systematics and Evolution2019，57，6： | 7 |
| 15 | Novel roll effector based on zero-mass-flux dual synthetic jets and its flight test显示文摘The autonomous and controllable Dual Synthetic Jet Actuator(DSJA)is firstly integrated into the Unmanned Aerial Vehicle(UAV),and flight tests without the deflection of rudders are carried out to verify the viability of DSJA to control the attitudes of UAV during cruising.DSJA is improved into an actuator with two diaphragms and three cavities,which has higher energy levels.Actuators,differentially distributed on both sides of the wings,are installed on the trailing edge close to the wing tips.Flight tests,containing Differential Circulation Control(DCC)using double-side actuators,Positive Circulation Control(PCC)using left-side actuators and Negative Circulation Control(NCC)using right-side actuators,are implemented at cruising speed of 25 m/s.Results show that roll attitude control without rudders could be realized by DSJAs.DCC and NCC can generate the rightward roll and yaw angular velocity,prompting UAV to turn right.The stronger controlling ability can be achieved by DCC,with the maximum roll angular velocity of 15.62(°)/s.PCC can generate a rightward roll moment,but a leftward yaw moment will be produced at the same time.Leftward yaw induces the leftward rolling moment,which weakens the roll control effect,making UAV keep to yaw to the left with a small slope. | Zhenbing LUO Zhijie ZHAO Jiefu LIU Xiong DENG Mu ZHENG Hang YANG Qingyang CHEN Shiqing LI | 2022 | Chinese Journal of Aeronautics2022，35，8： | 7 |
| 16 | De novo assembly and comparative analysis of root transcriptomes from different varieties of Panax ginseng C. A. Meyer grown in different environments显示文摘Panax ginseng C. A. Meyer is an important traditional herb in eastern Asia. It contains ginsenosides, which are primary bioactive compounds with medicinal properties. Although ginseng has been cultivated since at least the Ming dynasty to increase production, cultivated ginseng has lower quantities of ginsenosides and lower disease resistance than ginseng grown under natural conditions. We extracted root RNA from six varieties of fifth-year P. ginseng cultivars representing four different growth conditions, and performed Illumina paired-end sequencing. In total, 163,165,706 raw reads were obtained and used to generate a de novo transcriptome that consisted of 151,763 contigs(76,336 unigenes), of which 100,648 contigs(66.3%) were successfully annotated. Differential expression analysis revealed that most differentially expressed genes(DEGs) were upregulated(246 out of 258, 95.3%) in ginseng grown under natural conditions compared with that grown under artificial conditions. These DEGs were enriched in gene ontology(GO) terms including response to stimuli and localization. In particular, some key ginsenoside biosynthesis-related genes, including HMG-Co A synthase(HMGS), mevalonate kinase(MVK), and squalene epoxidase(SE), were upregulated in wild-grown ginseng. Moreover, a high proportion of disease resistance-related genes were upregulated in wild-grown ginseng. This study is the first transcriptome analysis to compare wild-grown and cultivated ginseng, and identifies genes that may produce higher ginsenoside content and better disease resistance in the wild; these genes may have the potential to improve cultivated ginseng grown in artificial environments. | ZHEN Gang ZHANG Lei DU YaNan YU RenBo LIU XinMin CAO FangRui CHANG Qi DENG Xing Wang XIA Mian HE Hang | 2015 | Science China(Life Sciences)2015，58，11： | 6 |
| 17 | Prevention of surgical site infection under different ventilation systems in operating room environment显示文摘Biological particles in the operating room(OR)air environment can cause surgical site infections(SSIs).Various ventilation systems have been employed in ORs to ensure an ultraclean environment.However,the effect of different ventilation systems on the control of bacteria-carrying particles(BCPs)released from the surgical staff during surgery is unclear.In this study,the performance of four different ventilation systems(vertical laminar airflow ventilation(VLAF),horizontal laminar airflow ventilation(HLAP),differential vertical airflow ventilation(DVAF),and temperature-controlled airflow ventilation(TAF))used in an OR was evaluated and compared based on the spatial BCP concentration.The airflow field in the OR was solved by the Renormalization Group(RNG)k-e turbulence model,and the BCP phase was calculated by Lagrangian particle tracking(LPT)and the discrete random walk(DRW)model.It was found that the TAF system was the most effective ventilation system among the four ventilation systems for ensuring air cleanliness in the operating area.This study also indicated that air cleanliness in the operating area depended not only on the airflow rate of the ventilation system but also on the airflow distribution,which was greatly affected by obstacles such as surgical lamps and surgical staff. | Zhijian Liu Haiyang Liu Hang Yin Rui Rong Guoqing Cao Qihong Deng | 2021 | Frontiers of Environmental Science & Engineering2021，15，3： | 5 |
| 18 | Plant diversity in Yunnan:Current status and future directions显示文摘Yunnan,located in southwestern China,harbors more than 19,000 higher plants,which represents the highest plant diversity in the country.However,plant diversity in Yunnan faces enormous threats today,including habitat destruction and fragmentation,environmental pollution,and over-exploitation of natural resources.Despite recent efforts to protect biodiversity,there are still thousands of threatened species,some of which have become extinct.We analyzed available data to gain a greater understanding of plant diversity and the status of plant conservation in Yunnan.We found that southern,southeastern,and northwestern Yunnan are hotspots of total species,endemic species,specimens,new species and threatened species,whereas southeastern Yunnan is a hotspot for plant species with extremely small populations.Moreover,we found that there are still conservation gaps and poorly protected areas in central,eastern,and northeastern Yunnan.We conclude that conservation of plant diversity in Yunnan requires modern field investigation,systematic research,the development of comprehensive databases,and government support.We recommend that conservationists pay more attention to building and improving functional protection systems and popularizing science. | Li-Shen Qian Jia-Hui Chen Tao Deng Hang Sun | 2020 | Plant Diversity2020，42，4： | 5 |
| 19 | Lipid nanoparticle-encapsulated mRNA antibody provides longterm protection against SARS-CoV-2 in mice and hamsters显示文摘Monoclonal antibodies represent important weapons in our arsenal to against the CO VID-19 pandemic.However,this potential is severely limited by the time-consuming process of developing effective antibodies and the relative high cost of manufacturing.Herein,we present a rapid and cost-effective lipid nanoparticle(LNP)encapsulated-mRNA platform for in vivo delivery of SARS-CoV-2 neutralization antibodies.Two mRNAs encoding the light and heavy chains of a potent SARS-CoV-2 neutralizing antibody HB27,which is currently being evaluated in clinical trials,were encapsulated into clinical grade LNP formulations(named as mRNA-HB27-LNP).I n vivo characterizatio n dem on strated that intravenous administratio n of mRNA-HB27-LNP in mice resulted in a longer circulating half-life compared with the original HB27 antibody in protein format.More importantly,a single prophylactic administration of mRNA-HB27-LNP provided protection against SARS-CoV-2 challenge in mice at 1,7 and even 63 days post administration.In a close contact transmission model,prophylactic administration of mRNA-HB27-LNP prevented SARS-CoV-2 in fecti on betwee n hamsters in a dose-depende nt mariner.Overall,our results dem on strate a superior Ion g-term protect!on agai nst SARS-CoV-2 conferred by a single administration of this unique mRNA antibody,highlight!ng the potential of this universal platform for antibody-based disease prevention and therapy against COVID-19 as well as a variety of other infectious diseases. | Yong-Qiang Deng Na-Na Zhang Yi-Fei Zhang Xia Zhong Sue Xu Hong-Ying Qiu Tie-Cheng Wang Hui Zhao Chao Zhou Shu-Long Zu Qi Chen Tian-Shu Cao Qing Ye Hang Chi Xiang-Hui Duan Dan-Dan Lin Xiao-Jing Zhang Liang-Zhi Xie Yu-Wei Gao Bo Ying Cheng-Feng Qin | 2022 | Cell Research2022，32，4： | 5 |
| 20 | Application of waste frying oils in the biosynthesis of biodemulsifier by a demulsifying strain Alcaligenes sp.S-XJ-1显示文摘Exploration of biodemulsifiers has become a new research aspect.Using waste frying oils(WFOs) as carbon source to synthesize biodemulsifiers has a potential prospect to decrease production cost and to improve the application of biodemulsifiers in the oilfield.In this study,a demulsifying strain,Alcaligenes sp.S-XJ-1,was investigated to synthesize a biodemulsifier using waste frying oils as carbon source.It was found that the increase of initial pH of culture medium could increase the biodemulsifier yield but decrease the demulsification ratio compared to that using paraffin as carbon source.In addition,a biodemulsifier produced by waste frying oils and paraffin as mixed carbon source had a lower demulsification capability compared with that produced by paraffin or waste frying oil as sole carbon source.Fed-batch fermentation of biodemulsifier using waste frying oils as supplementary carbon source was found to be a suitable method.Mechanism of waste frying oils utilization was studied by using tripalmitin,olein and tristearin as sole carbon sources to synthesize biodemulsifier.The results showed saturated long-chain fatty acid was diffcult for S-XJ-1 to utilize but could effectively enhance the demulsification ability of the produced biodemulsifier.Moreover,FT-IR result showed that the demulsification capability of biodemulsifiers was associated with the content of C=O group and nitrogen element. | Jia Liu Kaiming Peng Xiangfeng Huang Lijun Lu Hang Cheng Dianhai Yang Qi Zhou Huiping Deng | 2011 | Journal of Environmental Sciences2011，23，6： | 4 |
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