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| 1 | Neural stem cell transplantation in a double-layer collagen membrane with unequal pore sizes for spinal cord injury repair显示文摘A novel double-layer collagen membrane with unequal pore sizes in each layer was designed and tested in this study.The inner,loose layer has about 100-μm-diameter pores,while the outer,compact layer has about 10-μm-diameter pores.In a rat model of incomplete spinal cord injury,a large number of neural stem cells were seeded into the loose layer,which was then adhered to the injured side,and the compact layer was placed against the lateral side.The results showed that the transplantation of neural stem cells in a double-layer collagen membrane with unequal pore sizes promoted the differentiation of neural stem cells,attenuated the pathological lesion,and significantly improved the motor function of the rats with incomplete spinal cord injuries.These experimental findings suggest that the transplantation of neural stem cells in a double-layer collagen membrane with unequal pore sizes is an effective therapeutic strategy to repair an injured spinal cord. | Ning Yuan Wei Tian Lei Sun Runying Yuan Jianfeng Tao Dafu Chen | 2014 | Neural Regeneration Research2014，9，10： | 7 |
| 2 | Beneficial effects of C-peptide on renal morphology in diabetic rats显示文摘学习被承担在 glomerular 上检验 C 肽的效果在有差或中等的 glycemic 的糖尿病的老鼠控制的 streptozotocin (STZ ) 的体积(VGLOM ) , mesangial 矩阵合成,和降级。(差的 glycemic 控制) 系列 1 包括了一些健康老鼠, hyperglycemic 老鼠,糖尿病的对待胰岛素的老鼠和糖尿病的 C-peptide-treated 老鼠。(中等 glycemic 控制) 系列 2 包括了一些健康老鼠,糖尿病的对待胰岛素的老鼠,糖尿病的 insulin-peptide-treated 和 C-peptide-treated 老鼠。在 8 个星期以后,左肾经由轻显微镜学为 VGLOM 和 mesangial 矩阵区域的评估被切除。Mesangial 房间是有教养的因为 48 h 和类型 IV 骨胶原表示和矩阵 metalloproteinase (MMP )-2 表示被 ELISA 和 RTPCR 测量。结果显示在系列 1, C 肽管理在 VGLOM 和 mesangial 矩阵区域压制了导致糖尿病的增加。在系列 2, C 肽管理在 mesangial 矩阵区域在 VGLOM 和更大的减少导致了类似的减少什么时候与胰岛素治疗相比独自一个。而且, C 肽(300 nM ) 完全在在 30 公里葡萄糖媒介有教养的 mesangial 房间禁止了骨胶原 IV mRNA 表示和蛋白质集中的导致葡萄糖的增加。MMP-2 mRNA 表示没被 C 肽影响。在结论,到为 8 个星期的 STZ 糖尿病的老鼠的 C 肽管理导致 mesangial 矩阵的导致糖尿病的扩大的抑制。这效果独立于 glycemic 控制的水平,从 mesangial 的导致糖尿病的过多的形成的抑制的结果打 IV 骨胶原。 | Wei Sun Xin Gao Xiaolong Zhao Dafu Cui Qichang Xia | 2010 | Acta Biochimica et Biophysica Sinica2010，42，12： | 3 |
| 3 | A single-cell transcriptome of mesenchymal stromal cells to fabricate bioactive hydroxyapatite materials for bone regeneration显示文摘The osteogenic microenvironment of bone-repairing materials plays a key role in accelerating bone regeneration but remains incompletely defined,which significantly limits the application of such bioactive materials.Here,the transcriptional landscapes of different osteogenic microenvironments,including three-dimensional(3D)hydroxyapatite(HA)scaffolds and osteogenic medium(OM),for mesenchymal stromal cells(MSCs)in vitro were mapped at single-cell resolution.Our findings suggested that an osteogenic process reminiscent of endochondral ossification occurred in HA scaffolds through sequential activation of osteogenic-related signaling pathways,along with inflammation and angiogenesis,but inhibition of adipogenesis and fibrosis.Moreover,we revealed the mechanism during OM-mediated osteogenesis involves the ZBTB16 and WNT signaling pathways.Heterogeneity of MSCs was also demonstrated.In vitro ossification of LRRC75A+MSCs was shown to have better utilization of WNT-related ossification process,and PCDH10+MSCs with superiority in hydroxyapatite-related osteogenic process.These findings provided further understanding of the cellular activity modulated by OM conditions and HA scaffolds,providing new insights for the improvement of osteogenic biomaterials.This atlas provides a blueprint for research on MSC heterogeneity and the osteogenic microenvironment of HA scaffolds and a database reference for the application of bioactive materials for bone regeneration. | Peng Guo Xizhe Liu Penghui Zhang Zhongyuan He Zhen Li Mauro Alini RGeoff Richards Sibylle Grad Martin J.Stoddart Guangqian Zhou Xuenong Zou Danny Chan Wei Tian Dafu Chen Manman Gao Zhiyu Zhou Shaoyu Liu | 2022 | Bioactive Materials2022，7，3： | 3 |
| 4 | A New View of the Initiation and Propagation in Anionic Polymerization显示文摘这个工作证实开始和 anionic 聚合的繁殖机制的新看法以前求婚了,基于在自我的帮助下的苯乙烯和甲基苯乙烯的 anionic 体积聚合的调查设计了 microflow 设备并且由 GPC 并且在 situ 7Li NMR 描绘了。趋于形成聚集 hexameric 的结构并且弄平基于前者形成巨大的聚集的结构,被发现那 n-BuLi。开始者的这些聚集能直接开始 anionic 聚合并且形成 supramolecule 聚集。supramolecule 聚集不可避免地堵住了单体的散开到离子对并且导致了一个静止变换的平台。然后,集成者完全被分裂进相等的聚集二进制代码的种类,并且聚合在结束前很快再继续。Tetrahydrofuran (THF ) 充当了电子捐赠者,它能把电子云推到李阳离子并且做相当松开并且便于单体插入在里面的活跃种类的聚集的戒指。因此,小 THF 能极大地支持 anionic 聚合。然而, THF 的进一步的增加可能堵住在离子对和减少之间的隧道繁殖率。活跃种类的聚集的结构仅仅在 anionic 聚合期间取决于在聚合前被形成的开始者聚集,这也被发现。 | Anna Zheng Bo Chen Dihang Hu Yong Guan Dafu Wei Shuzhao Li Dandan Ji | 2013 | Chinese Journal of Chemistry2013，31，3： | 2 |
| 5 | Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration显示文摘It has been proven that the mechanical microenvironment can impact the differentiation of mesenchymal stem cells(MSCs).However,the effect of mechanical stimuli in biofabricating hydroxyapatite scaffolds on the inflammatory response of MSCs remains unclear.This study aimed to investigate the effect of mechanical loading on the inflammatory response of MSCs seeded on scaffolds.Cyclic mechanical loading was applied to biofabricate the cell-scaffold composite for 15 min/day over 7,14,or 21 days.At the predetermined time points,culture supernatant was collected for inflammatory mediator detection,and gene expression was analyzed by qRT-PCR.The results showed that the expression of inflammatory mediators(IL1B and IL8)was downregulated(p<0.05)and the expression of ALP(p<0.01)and COL1A1(p<0.05)was upregulated under mechanical loading.The cell-scaffold composites biofabricated with or without mechanical loading were freeze-dried to prepare extracellular matrix-based scaffolds(ECM-based scaffolds).Murine macrophages were seeded on the ECM-based scaffolds to evaluate their polarization.The ECM-based scaffolds that were biofabricated with mechanical loading before freeze-drying enhanced the expression of M2 polarization-related biomarkers(Arginase 1 and Mrc1,p<0.05)of macrophages in vitro and increased bone volume/total volume ratio in vivo.Overall,these findings demonstrated that mechanical loading could dually modulate the inflammatory responses and osteogenic differentiation of MSCs.Besides,the ECM-based scaffolds that were biofabricated with mechanical loading before freeze-drying facilitated the M2 polarization of macrophages in vitro and bone regeneration in vivo.Mechanical loading may be a promising biofabrication strategy for bone biomaterials. | Penghui Zhang Xizhe Liu Peng Guo Xianlong Li Zhongyuan He Zhen Li Martin J.Stoddart Sibylle Grad Wei Tian Dafu Chen Xuenong Zou Zhiyu Zhou Shaoyu Liu | 2021 | Bioactive Materials2021，6，10： | 2 |
| 6 | STUDY OF CAPILLARY ELECTROPHORESIS ON MICROCHIP BASED ON MEMS显示文摘Using a standard photolithographical procedure, chemical wet etching and thermal diffusion bonding technology, a chemical analysis device for Capillary Electrophoresis(CE) has been microfabricated on a planar glass substrate with a cross-column geometry. The channels on the microchip substrate are about 50μm deep and 150μm wide. By employing amino acids derived from 2,4-DiNitroFluoroBenzen (DNFB) on CE chip channels, the sample manipulating system is studied based on the principle of electrodynamics. | Wang Ming Li Wei Han Jinghong Cui Dafu (State Key Lab. of Transducer Tech., Inst. of Electron., Chinese Academy of Sci., Beijing 100080) | 2002 | Journal of Electronics(China)2002，19，4： | 1 |
| 7 | Improving Foamability of Polypropylene by Grafting Modification显示文摘 | LIU Chunsheng WEI Dafu ZHENG Anna | 2006 | Journal of Applied Polymer Science2006，101，6： | 1 |
| 8 | 显示文摘 | Zheng Anna Yah Xue Wei Dafu | 2013 | Materials Science and Engineering2013，33，1： | 1 |
| 9 | The melt grafting preparation and rheological characterization of long chain branching polypropylene显示文摘 | Li Shuzhao Xiao Miaomiao Wei Dafu | 2009 | Polymer2009，50，25： | 1 |
| 10 | The Melt Grafting Preparation and Rheological Characterization of Long Chain Branching Polypropylene 显示文摘 | Shuzhao Li Miaomiao Xiao Dafu Wei | 2009 | Polymer2009，50，25： | 1 |
| 11 | The moll grafting preparation and rheological characterization of long chain branching polypropylene 显示文摘 | LI Shuzhao XIAO Miaomiao WEI Dafu | 2009 | Polymer2009，50，25： | 1 |
| 12 | Structural characterization and antibacterial activity of oligoguanidine (polyhexamethylene guanidine hydrochloride)显示文摘 | Dafu Wei Qiangxiang Ma Yong Guan Fuzeng Hu Anna Zheng Xi Zhang Zheng Teng Hua Jiang | 2009 | Materials Science & Engineering C2009，，6： | 1 |
| 13 | Structural characterization and antibacterial activity of oligoguanidine (polyhexamethylene guanidine hydrochloride)显示文摘 | Wei Dafu Ma Qiangxiang Guan Yong | 2009 | Materials Science and Engineering C2009，29，6： | 1 |
| 14 | Structural characterization and antibacterial activity of oligoguanidine (polyhexamethylene guanidine hydrochloride) 显示文摘 | Wei Dafu Ma Qiangxiang Guan Yong | 2009 | Materials Science and Engineering C2009，29，6： | 1 |
| 15 | Damage of Escherichia coli membrane by bactericidal agent polyhexa methylene guanidine hydrochloride: Micrographie evidences 显示文摘 | Zhou Zhongxin Wei Dafu Guan Yong | 2010 | Journal of Applied Microbiology2010，108，3： | 1 |
| 16 | Structural characterization and antibacterial activity of oligoguanidine (polyhexamethylene guanidine hydrochloride)显示文摘 | Dafu Wei Qiangxiang Ma Yong Guan Fuzeng Hu Anna Zheng Xi Zhang Zheng Teng Hua Jiang | 2009 | Materials Science & Engineering C2009，，6： | 1 |
| 17 | The melt grafting preparation and rheological characterization of long chain branching polypropylene显示文摘 | Shuzhao Li Miaomiao Xiao Dafu Wei Huining Xiao Fuzeng Hu Anna Zheng | 2009 | Polymer2009，，25： | 1 |
| 18 | Structural characterization and antibacterial activity of oligoguanidine (polyhexamethylene guanidine hydrochloride)显示文摘 | Wei Dafu Ma Qiangxiang Guan Yong | 2009 | Materials Science and Engineering C2009，29，6： | 1 |
| 19 | Structural characterization and antibacterial activity of oligoguanidine (polyhexamethylene guanidine hydrochloride)显示文摘 | Wei Dafu Ma Qiangxiang Guan Yong | 2009 | Mater Set Eng C2009，29，6： | 1 |
| 20 | Synthesis of Block Copolymers of 2-Ethylhexyl Methacrylate, n-Hexyl Methacrylate and Methyl Methacrylate via Anionic Polymerization at Ambient Temperature显示文摘 | Guijin Zou Anna Zheng Dafu wei Zheng Li Ling Su Tongyuan Zhang Xiang Xu Yong Guan | 2018 | Chinese Journal of Chemistry2018，36，10： | 0 |
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