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| 1 | Differentiation of human embryonic stem cells derived mesenchymal stem cells into corneal epithelial cells after being seeded on decellularized SMILE-derived lenticules显示文摘AIM: To evaluate the feasibility of mesenchymal stem cells(MSCs) to differentiate into corneal epithelial cells after being seeded on the decellularized small incision lenticule extraction(SMILE)-derived lenticules. METHODS: The fresh lenticules procured from patients undergoing SMILE for the correction of myopia were decellularized. The MSCs were subsequently cultivated on those denuded lenticules. The MSCs without lenticules were used as a control. The proliferation activity of the MSCs after seeding 24 h was quantitatively determined with the Cell Counting Kit-8(CCK-8) assay. Immunofluorescence staining and quantitative reverse transcription polymerase chain reaction(qRT-PCR) were used to assess the marker expression in differentiated MSCs. RESULTS: The data showed that both fresh and decellularized lenticules could significantly promote the proliferation of MSCs, compared to that in control(P=0.02 for fresh lenticules, P=0.001 for decellularize ones, respectively). The MSCs seeded on both lenticules were positive for cytokeratin 3(CK3) staining. The expression of CK3 increased 5-fold in MSCs seeded on fresh lenticules and 18-fold on decellularized ones, compared to that in control. There was a significant difference in the expression of CK3 in MSCs seeded on fresh and decellularized lenticules(P<0.001). The expression of CK8 and CK18 was similar in pure MSCs and MSCs seeded on fresh lenticules(P>0.05), while the expression of these markers was decreased in MSCs seeded on decellularized ones. CONCLUSION: These results suggest that the decellularized lenticules might be more suitable for MSCs to differentiate into corneal epithelial cells, which offersthe prospect of a novel therapeutic modality of SMILEderived lenticules in regenerative corneal engineering. | Yao Chen Ye-Wei Yin Yang Zhao Xiao-Ying Wu Kelly Young Wei-Tao Song Xiao-Bo Xia Dan Wen | 2019 | International Journal of Ophthalmology(English edition)2019，12，5： | 2 |
| 2 | Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin显示文摘 | Dan C Kelly A Bernard O Minden A | 2001 | J Biol Chem2001，276，32： | 1 |
| 3 | Intelligent resource discovery using ontology-based resource profile 显示文摘 | J Steven Hughes Dan Crichton Scan Kelly Chris A Mattmarm Jerry Crichton | 2005 | Data Science Journal (S1683-1740)2005，4，31： | 1 |
| 4 | Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are media- ted by LIM kinase 1 and cofilin显示文摘 | Dan C Kelly A Bernard O | 2001 | J Biol Chem2001，276，32： | 1 |
| 5 | Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin显示文摘 | Dan C Kelly A Bernard O | 2001 | J Biol Chem2001，276，32： | 1 |
| 6 | The role of the mannose receptor in a murine model of Cryptococcus neoformans infection显示文摘 | Dan JM Kelly RM Lee CK | 2008 | Infect Immun2008，76，6： | 1 |
| 7 | Role of the mannose receptor in a murine model of Cryptococcus neoformans infection显示文摘 | Dan JM Kelly RM Lee CK Levitz SM | 2008 | Infect Immun2008，76，6： | 1 |
| 8 | Hierarchical reliability in experimental plant assemblages显示文摘Aims The mechanism by which species richness affects variation in ecosystem functioning both within and among ecosystems remains a key question at the interface of community and ecosystem ecology.Statistical averaging(the smoothing of average system performance via consideration of additional components)and the insurance effect(reduced variation in system performance by inclusion of asynchronously varying components)predict that more diverse communities should vary less both between replicates and internally.We experimentally tested these theories in small plant assemblages.Methods We constructed plant assemblages modeled after old-field plant communities.We varied species richness,species composition and initial densities while holding functional group richness constant in replicate assemblages under glasshouse conditions.Important findings The inverse of the coefficient of variation of aboveground biomass production,a proxy measure of reliability,increased with higher diversity when examined at the level of the assemblage(i.e.among-replicate assemblages)but not at the levels of functional group or species.These stabilizing processes were weakest in low-diversity,low-density assemblages.This experiment demonstrates the utility of hierarchical analysis of ecosystem reliability at the assemblage,functional group and species level. | Dan F.B.Flynn Bernhard Schmid Jin-Sheng He Kelly S.Wolfe-Bellin F.A.Bazzaz | 2008 | Journal of Plant Ecology2008，1，1： | 1 |
| 9 | Role of the mannose receptor in a murine model Cryptococcus neoformans infection显示文摘 | Dan JM Kelly RM Lee CK | 2008 | Infect Immun2008，76，6： | 1 |
| 10 | A 3--V 340row 14-b 75-Msamp]e/s CMOS ADC with 85--dB SFDI at Nyquist lnput显示文摘 | Wenhua Yang Dan Kelly luri Mehr | 2001 | lEEE Journal of Solid-State Circuits2001，36，19： | 1 |
| 11 | Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin显示文摘 | DAN C KELLY A BERNARD O | 2001 | J Biol Chem2001，276，32： | 1 |
| 12 | Cytoskeletal changesregulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin显示文摘 | Dan C Kelly A Bernard O | 2001 | J Biol Chem2001，276，32： | 1 |
| 13 | Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase1 and cofilin显示文摘 | Dan C Kelly A Bernard O | 2001 | J Biol Chem2001，276，32： | 1 |
| 14 | A 3 - V 340 - mW 14 - b 75 - Msample/s CMOS ADC with 85 - dB SFDR at nyquist Input 显示文摘 | Wenhua Yang Dan Kelly Iuri Mehr | 2001 | IEEE journal of solid - state circuits2001，36，12： | 1 |
| 15 | A critical examination of the thermodynamic of water adsorption on actinide oxide surfaces 显示文摘 | Paffett M T Dan Kelly Joyce S A | 2003 | J Nucl Mater2003，322，： | 1 |
| 16 | Wound Complications after Total Knee Arthroplasty显示文摘 | Kelly Vince Dan Chivas Kurt P. Droll | 2007 | The Journal of Arthroplasty2007，，4： | 1 |
| 17 | A Critical Examination of the Thermodynamic of Water Adsorption on Actinide Oxide Surfaces显示文摘 | Paffett M T Dan Kelly Joyee S A | 2003 | J Nucl Mater2003，322，： | 1 |
| 18 | Proton pump inhibitor therapy for chronic laryngo-pharyngitis: A randomized placebo-control trial显示文摘 | David L. Steward Keith M. Wilson Dan H. Kelly Mona S. Patil Heather R. Schwartzbauer John D. Long Jeff A. Welge | 2004 | Otolaryngology - Head and Neck Surgery2004，，4： | 1 |
| 19 | Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin显示文摘 | Dan C Kelly A Bernard O | 2001 | J Biol Chem2001，276，32： | 1 |
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