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您的检索式：作者名="Daniel Stoecklein"
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| 1 | Prognostic Significance of EpCAM-Positive Disseminated Tumor Cells in Rectal Cancer Patients With Stage I Disease显示文摘 | Sameer Dhayat Sorina Sorescu Daniel Vallb?hmer Sebastian Kraus Stephan Ernst Baldus Alexander Rehders Feride Kr?pil Andreas Krieg Wolfram Trudo Knoefel Nikolas Hendrik Stoecklein | 2012 | The American Journal of Surgical Pathology2012，，12： | 1 |
| 2 | Shaped 3D microcarriers for adherent cell culture and analysis显示文摘Standard tissue culture of adherent cells is known to poorly replicate physiology and often entails suspending cells in solution for analysis and sorting,which modulates protein expression and eliminates intercellular connections.To allow adherent culture and processing in flow,we present 3D-shaped hydrogel cell microcarriers,which are designed with a recessed nook in a first dimension to provide a tunable shear-stress shelter for cell growth,and a dumbbell shape in an orthogonal direction to allow for self-alignment in a confined flow,important for processing in flow and imaging flow cytometry.We designed a method to rapidly design,using the genetic algorithm,and manufacture the microcarriers at scale using a transient liquid molding optofluidic approach.The ability to precisely engineer the microcarriers solves fundamental challenges with shear-stress-induced cell damage during liquid-handling,and is poised to enable adherent cell culture,in-flow analysis,and sorting in a single format. | Chueh-Yu Wu Daniel Stoecklein Aditya Kommajosula Jonathan Lin Keegan Owsley Baskar Ganapathysubramanian Dino Di Carlo | 2018 | Microsystems & Nanoengineering2018，4，1： | 1 |
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