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| 1 | Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits显示文摘Aegilops tauschii is the donor of the D genome of common wheat (Triticum aestivum). Genetic variation of HMW glutenin subunits encoded by the Glu-1Dt locus of Ae. tauschii has been found to be higher than that specified by the Glu-1D locus in common wheat. In the present note, we report the identification of a novel HMW glutenin subunit, Dy13t, from Ae. tauschii. The newly identified subunit pos-sessed an electrophoretic mobility that was faster than that of the Dy12 subunit of common wheat. The complete ORF of encoding the Dy13t subunit contained 624 codons (excluding the stop codons). The amino acid sequence deduced from the Dy13t gene ORF was the shortest among those of the previ-ously reported subunits derived by the D genome. A further comparison of Dy13t amino acid sequence with those of the subunits characterized from the A, B, D, R genomes of Triticeae showed that the smaller size of the Dy13t subunit was associated with a reduction in the size of its repetitive domain. | YAN Zehong WAN Yongfang LIU Kunfan ZHENG Youliang WANG Daowen | 2002 | Chinese Science Bulletin2002，47，3： | 60 |
| 2 | Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana显示文摘Abiotic stresses cause serious crop losses. Knowledge on genes functioning in plant responses to adverse growth conditions is essential for developing stress tolerant crops. Here we report that transgenic expression of MYB15, encoding a R2R3 MYB transcription factor in Arabidopsis thaliana, conferred hypersensitivity to exogenous abscisic acid (ABA) and improved tolerance to drought and salt stresses. The promoter of MYB15 was active in not only vegetative and reproductive organs but also the guard cells of stomata. Its transcript level was substantially upregulated by ABA, drought or salt treatments. Compared with wild type (WT) control, MYB15 overexpression lines were hypersensitive to ABA in germination assays, more susceptible to ABA-elicited inhibition of root elongation, and more sensitive to ABA-induced stomatal closure. In line with the above findings, the transcript levels of ABA biosynthesis (ABA1, ABA2), signaling (ABI3), and responsive genes (AtADH1, RD22, RD29B, AtEM6) were generally higher in MYB15 overexpression seedlings than in WT controls after treatment with ABA. MYB15 overexpression lines displayed improved survival and reduced water loss rates than WT control under water deficiency conditions. These overexpression lines also displayed higher tolerance to NaCl stress. Collectively, our data suggest that overexpression of MYB15 improves drought and salt tolerance in Arabidopsis possibly by enhancing the expression levels of the genes involved in ABA biosynthesis and signaling, and those encoding the stress-protective proteins. | Zhenhua Ding Shiming Li Xueli An Xin Liu Huanju Qin Daowen Wang | 2009 | Journal of Genetics and Genomics2009，36，1： | 42 |
| 3 | Wheat genomic study for genetic improvement of traits in China显示文摘Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s population.Over the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestication of wheat,and the genetic basis of agronomically important traits,which promote the breeding of elite varieties.In this review,we focus on progress that has been made in genomic research and genetic improvement of traits such as grain yield,end-use traits,flowering regulation,nutrient use efficiency,and biotic and abiotic stress responses,and various breeding strategies that contributed mainly by Chinese scientists.Functional genomic research in wheat is entering a new era with the availability of multiple reference wheat genome assemblies and the development of cutting-edge technologies such as precise genome editing tools,highthroughput phenotyping platforms,sequencing-based cloning strategies,high-efficiency genetic transformation systems,and speed-breeding facilities.These insights will further extend our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits and facilitate the breeding process,ultimately contributing to more sustainable agriculture in China and throughout the world. | Jun Xiao Bao Liu Yingyin Yao Zifeng Guo Haiyan Jia Lingrang Kong Aimin Zhang Wujun Ma Zhongfu Ni Shengbao Xu Fei Lu Yuannian Jiao Wuyun Yang Xuelei Lin Silong Sun Zefu Lu Lifeng Gao Guangyao Zhao Shuanghe Cao Qian Chen Kunpu Zhang Mengcheng Wang Meng Wang Zhaorong Hu Weilong Guo Guoqiang Li Xin Ma Junming Li Fangpu Han Xiangdong Fu Zhengqiang Ma Daowen Wang Xueyong Zhang Hong-Qing Ling Guangmin Xia Yiping Tong Zhiyong Liu Zhonghu He Jizeng Jia Kang Chong | 2022 | Science China(Life Sciences)2022，65，9： | 9 |
| 4 | An experiment-library least-squares method on on-line coal element contents analysis显示文摘A new experiment-library least-squares(experiment-LLs) method used in the neutron inelastic-scattering and thermal-capture analysis(NITA) technique for on-line coal analysis was developed,which has significantly decreased the non-linear radiation effects.In this method,sixty samples with preset elemental contents were made,from whose experimental spectra twenty single-element spectrum libraries corresponding to twenty kinds of coal were built using the least-squares method.The spectrum of the unknown sample was analyzed based on these twenty libraries to estimate its element contents.With the initial estimated result,the procedure of developing library and analysis was iterated to build a new library with the closest element contents to the unknown sample.Hence the experiment-LLs method can reduce non-linear radiation effects.The experiment-LLs method was performed on an improved coal analysis system which was equipped with a long-life 14 MeV pulsed-neutron generator,a bulk BGO detector with a temperature controller,a moisture meter and coal-smoothing device.The precisions of this system for ash content,water content,volatile content and calorific value have reached 1.0wt%,0.5wt%,1.0wt%,350 kJ/kg,respectively. | WANG HuiDong LU JingBin LU YuPing YANG Dong MA KeYan YANG Kang LIU YuMin CHENG DaoWen | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，11： | 6 |
| 5 | Aerosol observation in Fengtai area,Beijing显示文摘Measurements of aerosol number concentration and particulate matter with diameter less than 10 m(PM10) mass concentrations of urban background aerosols were performed in Fengtai area,Beijing in 2006.Black carbon(BC) was collected simultaneously from the ground and analyzed to determine the particulate matter components.To satisfy the interest in continuous monitoring of temporal and spatial distribution of aerosols,the relationship between extinction coefficient(visibility) measured by lidar remote sensing and the aerosol number concentration measured from the ground was derived by using statistical method.Vertical particle number concentration profile within the planetary boundary layer could be inversed through the lidar data as well as the statistical relation. | Zengdong Liu Jianguo Liu Bei Wang Fan Lu Shuhua Huang Dexia Wu Daowen Hart | 2008 | Particuology2008，6，3： | 6 |
| 6 | Pandemonium Breaks Out:Disruption of Salicylic Acid-Mediated Defense by Plant Pathogens显示文摘Salicylic acid (SA) or 2-hydroxybenoic acid is a phenolic plant hormone that plays an essential role in plant defense against biotrophic and semi-biotrophic pathogens. In Arabidopsis, SA is synthesized from chorismate in the chloroplast through the ICS1 (isochorismate synthase I) pathway during pathogen infectioru The transcription co-activator NPR1 (Non-Expresser of Pathogenesis-Related Gene 1), as the master regulator of SA signaling, interacts with transcription factors to induce the expression of anti-microbial PR (Pathogenesis-Related) genes. To establish successful in fections, plant bacterial, oomycete, fun gal, and viral pathogens have evolved at least three major strategies to disrupt SA-mediated defense? The first strategy is to reduce SA accumulation directly by converting SA into its inactive derivatives. The second strategy is to interrupt SA biosynthesis by targeting the ICS1 pathway. In the third major strategy, plant pathogens deploy different mechanisms to interfere with SA downstream signaling. The wide array of strategies deployed by plant pathogens highlights the crucial role of disruption of SA-mediated plant defense in plant pathogenesis. A deeper understanding of this topic will greatly expand our knowledge of how plant pathogens cause diseases and consequently pave the way for the development of more effective ways to corn trol these diseases. | Guang Qi Jian Chen Ming Chang Huan Chen Katherine Hall John Korin Fengquan Liu Daowen Wang Zheng Qing Fu | 2018 | Molecular Plant2018，11，12： | 5 |
| 7 | Molecular and Functional Characterization of Sphingosine-1-Phosphate Lyase Homolog from Higher Plants显示文摘Sphingosine-1-phosphate lyase (系统程序设计语言) 涉及降级表明分子 sph-ingosine-1-phosphate 的 conservedsphingolipid。从 Arabidopsis thaliana 和米饭的通常认为的系统程序设计语言蛋白质的生物信息、分子、功能的分析(指定了为 AtSPL 和 OsSPL,分别地) 被进行。氨基酸顺序比较表明植物系统程序设计语言包含了 thepyridoxal 依赖的脱羧酶领域和可以涉及底层催化作用的保存残余。当在 Saccharomyces cerevisiae 表示了时, AtSPL 和 OsSPL 改正了酵母 dpl1 删除紧张的过分敏感的显型,它在内长的系统程序设计语言活动是缺乏的,到长链基于的外长的 sphingolipid (LCB ) ,建议那植物系统程序设计语言蛋白质是在降级的 phosphorylated LCB 的功能的 invivo。在 A。thaliana, AtSPL 抄本在根,茎,叶子,花和 siliques 被检测。在 pAtSPL-AtSPL::GUS 转基因的线, AtSPL::GUS 熔化蛋白质在许多植物、繁殖的纸巾被发现。AtSPL 表示水平动态地在叶开发和老朽期间被调整,并且稳定地并且显著地在 A 被增加。thaliana 幼苗在 A 与房间对待系统程序设计语言的导致死亡的真菌的毒素 fumonisin B1.The 潜在的功能。thaliana 被讨论…… | Yan Niu Kunling Chen Jizhou Wang Xin Liu Huanju Qin Aimin Zhang Daowen Wang | 2007 | Journal of Integrative Plant Biology2007，49，3： | 3 |
| 8 | Overexpressing HRS1 Confers Hypersensitivity to Low Phosphate-Elicited Inhibition of Primary Root Growth in Arabidopsis thaliana显示文摘Phosphate (Pi) deficiency causes dramatic root system architecture(RSA) changes in higher plants.Here we report that overexpression of HRS1 leads to enhanced sensitivity to low Pi-elicited inhibition of primary root growth in Arabidopsis thaliana seedlings.Bioinformatic investigations uncovered that HRS1 and its six homologs encode putative G2-like transcription factors in Arabidopsis.Analysis of promoter::GUS reporter lines revealed that HRS1 transcripts were present mainly in the root hair region and root hair cells under Pi-sufficient conditions.Pi deprivation increased HRS1 expression level and expanded its expression domain.Although HRS1 knockout mutant did not differ from wild type(WT) control irrespective of Pi status,its overexpression lines were significantly more susceptible to low Pi-elicited primary root shortening.In both WT and HRS1 overexpression seedlings,low Pi-induced primary root shortening was accompanied by enhanced root hair cell differentiation,but this enhancement occurred to a greater extent in the latter genotype.Collectively,our data suggest that HRS1 may be involved in the modulation of primary root and root hair growth in Pi-deprived Arabidopsis seedlings,and provide useful clues for further research into the function of HRS1 and its homologs and the mechanisms behind RSA changes under Pi-deficient conditions. | Hong Liu Huixia Yang Chongming Wu Juanjuan Feng Xin Liu Huanju Qin Daowen Wang | 2009 | Journal of Integrative Plant Biology2009，51，4： | 3 |
| 9 | Genetic Analysis of Chromosomal Loci Affecting the Content of Insoluble Glutenin in Common Wheat显示文摘In common wheat, insoluble glutenin(IG) is an important fraction of flour glutenin macropolymers, and insoluble glutenin content(IGC)is positively associated with key end-use quality parameters. Here, we present a genetic analysis of the chromosomal loci affecting IGC with the data collected from 90 common wheat varieties cultivated in four environments. Statistical analysis showed that IGC was controlled mainly genetically and influenced by the environment. Among the major genetic components known to affect end-use quality,1BL/1RS translocation had a significantly negative effect on IGC across all four environments. As to the different alleles of Glu-A1,-B1 and-D1 loci, Glu-A1 a, Glu-B1 b and Glu-D1 d exhibited relatively strong positive effects on IGC in all environments. To identify new loci affecting IGC, association mapping with 1355 DAr T markers was conducted. A total of 133 markers were found associated with IGC in two or more environments(P < 0.05), ten of which consistently affected IGC in all four environments. The phenotypic variance explained by the ten markers varied from 4.66% to 8.03%, and their elite alleles performed significantly better than the inferior counterparts in enhancing IGC. Among the ten markers, w Pt-3743 and w Pt-733835 reflected the action of Glu-D1, and w Pt-664972 probably indicated the effect of Glu-A1. The other seven markers, forming three clusters on 2AL, 3BL or 7BL chromosome arms, represented newly identified genetic determinants of IGC. Our work provided novel insights into the genetic control of IGC, which may facilitate wheat enduse quality improvement through molecular breeding in the future. | Huaibing Jin Zhaojun Wang Da Li Peipei Wu Zhengying Dong Chaowu Rong Xin Liu Huanju Qin Huili Li Daowen Wang Kunpu Zhang | 2015 | Journal of Genetics and Genomics2015，42，9： | 3 |
| 10 | Constructing Large Scale Cohort for Clinical Study on Heart Failure with Electronic Health Record in Regional Healthcare Platform:Challenges and Strategies in Data Reuse显示文摘Regional healthcare platforms collect clinical data from hospitals in specific areas for the purpose of healthcare management.It is a common requirement to reuse the data for clinical research.However,we have to face challenges like the inconsistence of terminology in electronic health records (EHR) and the complexities in data quality and data formats in regional healthcare platform.In this paper,we propose methodology and process on constructing large scale cohorts which forms the basis of causality and comparative effectiveness relationship in epidemiology.We firstly constructed a Chinese terminology knowledge graph to deal with the diversity of vocabularies on regional platform.Secondly,we built special disease case repositories (i.e.,heart failure repository) that utilize the graph to search the related patients and to normalize the data.Based on the requirements of the clinical research which aimed to explore the effectiveness of taking statin on 180-days readmission in patients with heart failure,we built a large-scale retrospective cohort with 29647 cases of heart failure patients from the heart failure repository.After the propensity score matching,the study group (n=6346) and the control group (n=6346) with parallel clinical characteristics were acquired.Logistic regression analysis showed that taking statins had a negative correlation with 180-days readmission in heart failure patients.This paper presents the workflow and application example of big data mining based on regional EHR data. | Daowen Liu Liqi Lei Tong Ruan Ping He | 2019 | Chinese Medical Sciences Journal2019，34，2： | 2 |
| 11 | Divergence in homoeolog expression of the grain length-associated gene GASR7 during wheat allohexaploidization显示文摘Hexaploid wheat has triplicated homoeologs for most of the genes that are located in subgenomes A, B, and D. GASR7, a member of the Snakin/GASA gene family, has been associated with grain length development in wheat. However, little is known about divergence of its homoeolog expression in wheat polyploids. We studied the expression patterns of the GASR7 homoeologs in immature seeds in a synthetic hexaploid wheat line whose kernels are slender like those of its maternal parent(Triticum turgidum, AABB, PI 94655) in contrast to the round seed shape of its paternal progenitor(Aegilops tauschii, DD, AS2404). We found that the B homoeolog of GASR7 was the main contributor to the total expression level of this gene in both the maternal tetraploid progenitor and the hexaploid progeny, whereas the expression levels of the A and D homoeologs were much lower. To understand possible mechanisms regulating different GASR7 homoeologs, we firstly analyzed the promoter sequences of three homoeologous genes and found that all of them contained gibberellic acid(GA) response elements, with the TaG ASR7 B promoter(pT aG ASR7B) uniquely characterized by an additional predicted transcriptional enhancer. This was confirmed by the GA treatment of spikes where all three homoeologs were induced, with a much stronger response for TaG ASR7 B. McrB C enzyme assays showed that the methylation status at pT aG ASR7 D was increased during allohexaploidization, consistent with the repressed expression of TaG ASR7 D. For pT aG ASR7 A, the distribution of repetitive sequence-derived 24-nucleotide(nt) small interfering RNAs(siR NAs) were found which suggests possible epigenetic regulation because 24-nt siR NAs are known to mediate RNA-dependent DNA methylation. Our results thus indicate that both genetic and epigenetic mechanisms may be involved in the divergence of GASR7 homoeolog expression in polyploid wheat. | Dongdong Zhang Bingnan Wang Junmin Zhao Xubo Zhao Lianquan Zhang Dengcai Liu Lingli Dong Daowen Wang Long Mao Aili Li | 2015 | The Crop Journal2015，3，1： | 2 |
| 12 | The Judgment of Chaotic Detection System's State Based on the Lyapunov Exponent显示文摘 | Haina HU Daowen LIU | | 0，，29： | 1 |
| 13 | Preparation and Properties of Polyarylene Ether Nitrites/Multi-walled Carbon Nanotubes Composites显示文摘 | Xiaobo Liu Shengru Long Daowen Luo | 2008 | Materials Letters2008，62，： | 1 |
| 14 | Preparation and properties of polyarylene ether nitrites/multi-walled carbon nanotubes composites显示文摘 | LIU Xiaobo LONG Shengru LUO Daowen | 2008 | Mater Lett2008，62，： | 1 |
| 15 | Preparation and properties of polyarylene ether nitrites/multi-walled carbon nanotubes composites 显示文摘 | LIU Xiaobo LONG Shengru LUO Daowen | 2008 | Mater Lett2008，62，： | 1 |
| 16 | The BZR1-EDS1 module regulates plant growth-defense coordination显示文摘Plants have developed sophisticated strategies to coordinate growth and immunity,but our understanding of the underlying mechanism remains limited.In this study,we identified a novel molecular module that reg-ulates plant growth and defense in both compatible and incompatible infections.This module consisted of BZR1,a key transcription factor in brassinosteroid(BR)signaling,and EDS1,an essential positive regulator of plant innate immunity.We found that EDS1 interacts with BZR1 and suppresses its transcriptional activ-ities.Consistently,upregulation of EDS1 function by a virulent Pseudomonas syringae strain or salicylic acid treatment inhibited BZR1-regulated expression of BR-responsive genes and BR-promoted growth.Furthermore,we showed that the cytoplasmic fraction of BZR1 positively regulates effector-triggered im-munity(ETI)controlled by the TIR-NB-LRR protein RPS4,which is attenuated by BZR1's nuclear transloca-tion.Mechanistically,cytoplasmic BZR1 facilitated AvrRps4-triggered dissociation of EDS1 and RPS4 by binding to EDS1,thus leading to efficient activation of RPS4-controlled ETI.Notably,transgenic expression of a mutant BZR1 that accumulates exclusively in the cytoplasm improved pathogen resistance without compromising plant growth.Collectively,these results shed new light on plant growth-defense coordina-tion and reveal a previously unknown function for the cytoplasmic fraction of BZR1.The BZR1-EDS1 mod-ule may be harnessed for the simultaneous improvement of crop productivity and pathogen resistance. | Guang Qi Huan Chen Dian Wang Hongyuan Zheng Xianfeng Tang Zhengzheng Guo Jiayu Cheng Jian Chen Yiping Wang Ming-yi Bai Fengquan Liu Daowen Wang Zheng Qing Fu | 2021 | Molecular Plant2021，14，12： | 1 |
| 17 | Preparation and properties of polyarylene ether nitrites/multi-walled carbon nanotubes composites 显示文摘 | Liu Xiaobo Long Shengru Luo Daowen | 2008 | Materials Letters2008，62，2： | 1 |
| 18 | Preparation and properties of polyarylene ether nitrites/multi-walled carbon nanotubes composites 显示文摘 | Liu Xiaobo Long Shengru Luo Daowen | 2005 | Materials Letters2005，62，： | 1 |
| 19 | Preparation and properties of polyarylene ether nitrites/multi-walled carbon nanotubes composites 显示文摘 | LIU Xiaobo LONG Shengru LUO Daowen | 2008 | Mater Lett2008，62，1： | 1 |
| 20 | Prepa- ration and properties of polyarylene ether nitrites/muhi-walled carbon nanotubes composites 显示文摘 | LIU Xiaobo LONG Shengru LUO Daowen | 2008 | Mater Lett2008，62，1： | 1 |
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