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| 1 | Decreased expression of hyperpolarisation-activated cyclic nucleotide-gated channel 3 in Hirschsprung's disease显示文摘AIM: To determine if hyperpolarisation-activated nucleotide-gated(HCN) channels exist in human colon, and to investigate the expression of HCN channels in Hirschsprung's disease.METHODS:We investigated HCN1,HCN2,HCN3 and HCN4 protein expression in pull-through specimens from patients with Hirschsprung’s disease(HSCR,n=10)using the proximal-most ganglionic segment and distalmost aganglionic segment,as well as in healthy control specimens obtained at the time of sigmoid colostomy closure in children who had undergone anorectoplasty for imperforate anus(n=10).Fluorescent immunohistochemistry was performed to assess protein distribution,which was then visualized using confocal microscopy.RESULTS:No HCN1 channel expression was observed in any of the tissues studied.Both HCN2 and HCN4proteins were found to be equally expressed in the aganglionic and ganglionic bowel in HSCR and controls.HCN3 channel expression was found to be markedly decreased in the aganglionic colon vs ganglionic colon and controls.HCN2-4 channels were seen to be expressed within neurons of the myenteric and submucosal plexus of the ganglionic bowel and normal controls,and also co-localised to interstitial cells of Cajal in all tissues studied.CONCLUSION:We demonstrate HCN channel expression in human colon for the first time.Reduced HCN3expression in aganglionic bowel suggests its potential role in HSCR pathophysiology. | Anne Marie O’Donnell David Coyle Prem Puri | 2015 | World Journal of Gastroenterology2015，21，18： | 4 |
| 2 | Survey o{ Expert Opinion on Intelligence: Causes of International Differences in Cognitive Ability Tests显示文摘 | Heiner R David B Coyle T R | 2016 | Frontiers in Psychology2016，7，： | 1 |
| 3 | Forest production responses to irrigation and fertilization are not explained by shifts in allocation显示文摘 | David R. Coyle Mark D. Coleman | 2004 | Forest Ecology and Management2004，，1： | 1 |
| 4 | Altered distribution of small-conductance calcium-activated potassium channel SK3 in Hirschsprung’s disease显示文摘 | David Coyle Anne Marie O’Donnell Prem Puri | 2015 | Journal of Pediatric Surgery2015，，10： | 1 |
| 5 | Measurements of Ozone Deposition to Vegetation Quantifying the Flux, the Stomatal and Non-Stomatal Components显示文摘 | David Fowler Chris Flechard J. Neil Cape Robert L. Storeton-West Mhairi Coyle | 2001 | Water Air and Soil Pollution (-)2001，，1： | 1 |
| 6 | Review of the Toxicology of Chlorpyrifos With an Emphasis on Human Exposure and Neurodevelopment显示文摘 | David L. Eaton Robert B. Daroff Herman Autrup James Bridges Patricia Buffler Lucio G. Costa Joseph Coyle Guy McKhann William C. Mobley Lynn Nadel Diether Neubert Rolf Schulte-Hermann Peter S. Spencer | 2008 | Critical Reviews in Toxicology2008，，2： | 1 |
| 7 | Deficiency of platelet-derived growth factor receptor-α-positive cells in Hirschsprung's disease colon显示文摘AIM: To investigate whether the expression of plateletderived growth factor receptor-α-positive(PDGFRα^+)-cells is altered in Hirschsprung's disease(HD).METHODS: HD tissue specimens(n = 10) were collected at the time of pull-through surgery, while colonic control samples were obtained at the time of colostomy closure in patients with imperforate anus(n = 10). Immunolabelling of PDGFRα^+-cells was visualized using confocal microscopy to assess the distribution of these cells, while Western blot analysis was undertaken to quantify PDGFRα protein expression.RESULTS: Confocal microscopy revealed PDGFRα+-cells within the mucosa, myenteric plexus and smooth muscle in normal controls, with a marked reduction in PDGFRα^+-cells in the HD specimens. Western blotting revealed high levels of PDGFRα protein expression in normal controls, while there was a striking decrease in PDGFRα protein expression in the HD colon.CONCLUSION: These findings suggest that the altered distribution of PDGFRα^+-cells in both the aganglionic and ganglionic HD bowel may contribute to the motility dysfunction in HD. | Anne-Marie O'Donnell David Coyle Prem Puri | 2016 | World Journal of Gastroenterology2016，22，12： | 1 |
| 8 | An interactive numerical model of diode-pumped, Q-switched/ cavity-dumped lasers显示文摘 | D Barry Coyle David V Guerra Richard B Kay | 1995 | Applied Physics1995，28，： | 1 |
| 9 | Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung's-associated enterocolitis显示文摘AIM: To determine if expression of colonic tryptophan hydroxylase-2(TPH2), a surrogate marker of neuronal 5-hydroxytryptamine, is altered in Hirschsprung's-associated enterocolitis. METHODS: Entire resected colonic specimens were collected at the time of pull-through operation in children with Hirschsprung's disease(HSCR, n = 12). Five of these patients had a history of pre-operative Hirschsprung's-associated enterocolitis(HAEC). Controls were collected at colostomy closure in children with anorectal malformation(n = 10). The distribution of expression of TPH2 was evaluated using immunofluorescence and confocal microscopy. Protein expression of TPH2 was quantified using western blot analysis in the deep smooth muscle layers. RESULTS: TPH2 was co-expressed in nitrergic and cholinergic ganglia in the myenteric and submucosal plexuses in ganglionic colon in HSCR and healthy controls. Co-expression was also seen in submucosal interstitial cells of Cajal and PDGFRα+ cells. The density of TPH2 immuno-positive fibers decreased incrementally from ganglionic bowel to transition zonebowel to aganglionic bowel in the myenteric plexus. Expression of TPH2 was reduced in ganglionic bowel in those affected by pre-operative HAEC compared to those without HAEC and healthy controls. However, expression of TPH2 was similar or high compared to controls in the colons of children who had undergone diverting colostomy for medically refractory HAEC.CONCLUSION: Altered TPH2 expression in colonic serotonergic nerves of patients with HSCR complicated by HAEC may contribute to intestinal secretory and motor disturbances, including recurrent HAEC. | David Coyle Justin M Murphy Brian Doyle Anne Marie O’Donnell John Gillick Prem Puri | 2016 | World Journal of Gastroenterology2016，22，19： | 0 |
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