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| 1 | Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis显示文摘Iron has been shown to trigger oxidative stress by elevating reactive oxygen species (ROS) and to participate in different modes of cell death,such as ferroptosis,apoptosis and necroptosis. However,whether iron-elevated ROS is also linked to pyroptosis has not been reported. Here,we demonstrate that iron-activated ROS can induce pyroptosis via a Tom20-Bax-caspase-GSDME pathway. In melanoma cells,iron enhanced ROS signaling initiated by CCCP,causing the oxidation and oligomerization of the mitochondrial outer membrane protein Tom20. Bax is recruited to mitochondria by oxidized Tom20,which facilitates cytochrome c release to cytosol to activate caspase-3;eventually triggering pyroptotic death by inducing GSDME cleavage. Therefore,ROS acts as a causative factor and Tom20 senses ROS signaling for iron-driven pyroptotic death of melanoma cells. Since iron activates ROS for GSDME-dependent pyroptosis induction and melanoma cells specifically express a high level of GSDME,iron may be a potential candidate for melanoma therapy. Based on the functional mechanism of iron shown above,we further demonstrate that iron supplementation at a dosage used in iron-deficient patients is sufficient to maximize the anti-tumor effect of clinical ROS-inducing drugs to inhibit xenograft tumor growth and metastasis of melanoma cells through GSDME-dependent pyroptosis. Moreover,no obvious side effects are observed in the normal tissues and organs of mice during the combined treatment of clinical drugs and iron. This study not only identifies iron as a sensitizer amplifying ROS signaling to drive pyroptosis,but also implicates a novel iron-based intervention strategy for melanoma therapy. | Bo Zhou Jia-yuan Zhang Xian-shuo Liu Hang-zi Chen Yuan-li Ai Kang Cheng Ru-yue Sun Dawang Zhou Jiahuai Han Qiao Wu | 2018 | Cell Research2018，28，12： | 52 |
| 2 | The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8显示文摘Pyroptosis is a form of regulated cell death mediated by gasdermin family members,among which the function of GSDMC has not been clearly described.Herein,we demonstrate that the metabolite a-ketoglutarate(α-KG)induces pyroptosis through caspase-8-mediated cleavage of GSDMC Treatment with DM-αKG,a cell-permeable derivative of α-KG,elevates ROS levels,which leads to oxidation of the plasma membrane-localized death receptor DR6. | Jia-yuan Zhang Bo Zhou Ru-yue Sun Yuan-li Ai Kang Cheng Fu-nan Li Bao-rui Wang Fan-jian Liu Zhi-hong Jiang Wei-jia Wang Dawang Zhou Hang-zi Chen Qiao Wu | 2021 | Cell Research2021，31，9： | 10 |
| 3 | Facile Li-Al layered double hydroxide films on Al alloy for enhanced hydrophobicity, anti-biofouling and anti-corrosion performance显示文摘In this work,lithium(Li)-aluminum(Al)layered double hydroxide(LDH)films modified by 4-amino-2-((hydrazine methylene)amino)-4-oxobutanoic acid(denoted as AOA acid)and/or 1H,1H,2H,2H-perfluorooctyltriethoxysilane were prepared on 6N01 Al alloy by a facile,in-situ growth method with enhanced hydrophobicity,anti-biofouling and anti-corrosion performance.The preparation is low energy consumptive and environment friendly,relying on self-assembly at ambient temperature.The structure,molecular weight and functional groups of the synthesized AOA acid were characterized by NMR spectrometer,ESI-MS spectrometer and Fourier transform infrared(FT-IR)spectroscopy.And the compositions,structure and morphology of the films were characterized by Fourier transform infrared(FT-IR)spectroscopy,glancing-angle X-ray diffraction(GA-XRD),X-ray photoelectron spectroscopy(XPS),field emission scanning electron microscopy(FE-SEM)and energy-dispersive x-ray spectrum(EDS).Water contact angle measurements(CA)and atomic force microscopy(AFM)characterization show that the films possess a micro/nanostructure with an improved hydrophobicity.Immersion test,neutral salt tests(NSS)and electrochemical impedance spectroscopy(EIS)conducted in 3.5 wt.%NaCl solutions demonstrate the improved corrosion resistance of the films over bare Al alloy.Meanwhile,the films also possess an excellent anti-bacterial property toEscherichia coli,Bacillus subtilis and sulfate-reducing bacteria. | Ji Li Tianci Yuan Chengliang Zhou Bo Chen Yi Shuai Dawang Wu Dongchu Chen Xiaohu Luo Y.Frank Cheng Yali Liu | 2021 | Journal of Materials Science & Technology2021，，20： | 2 |
| 4 | Identification of serum metabolites enhancing inflammatory responses in COVID-19显示文摘Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is characterized by a strong production of inflammatory cytokines such as TNF and IL-6,which underlie the severity of the disease.However,the molecular mechanisms responsible for such a strong immune response remains unclear.Here,utilizing targeted tandem mass spectrometry to analyze serum metabolome and lipidome in COVID-19 patients at different temporal stages,we identified that 611 metabolites(of 1,039)were significantly altered in COVID-19 patients.Among them,two metabolites,agmatine and putrescine,were prominently elevated in the serum of patients;and 2-quinolinecarboxylate was changed in a biphasic manner,elevated during early COVID-19 infection but levelled off.When tested in mouse embryonic fibroblasts(MEFs)and macrophages,these 3 metabolites were found to activate the NF-κB pathway that plays a pivotal role in governing cytokine production.Importantly,these metabolites were each able to cause strong increase of TNF and IL-6 levels when administered to wildtype mice,but not in the mice lacking NF-κB.Intriguingly,these metabolites have little effects on the activation of interferon regulatory factors(IRFs)for the production of type I interferons(IFNs)for antiviral defenses.These data suggest that circulating metabolites resulting from COVID-19 infection may act as effectors to elicit the peculiar systemic inflammatory responses,exhibiting severely strong proinflammatory cytokine production with limited induction of the interferons.Our study may provide a rationale for development of drugs to alleviate inflammation in COVID-19 patients. | Chen-Song Zhang Bingchang Zhang Mengqi Li Xiaoyan Wei Kai Gong Zhiyong Li Xiangyang Yao Jianfeng Wu Cixiong Zhang Mingxia Zhu Lei Zhang Xiufeng Sun Yi-Hong Zhan Zhengye Jiang Wenpeng Zhao Wei Zhong Xinguo Zhuang Dawang Zhou Hai-Long Piao Sheng-Cai Lin Zhanxiang Wang | 2022 | Science China(Life Sciences)2022，65，10： | 1 |
| 5 | Efficient Photocatalytic Degradation of the Persistent PET Fiber‑Based Microplastics over Pt Nanoparticles Decorated N‑Doped TiO_(2) Nanoflowers显示文摘Fiber-based microplastics(FMPs)are highly persistent and ubiquitously exist in the wastewater of textile industry and urban sewage.It remains challenging to completely remove such newly emerged organic pollutants by the predominant physical techniques.In this work,we investigated a photocatalytic degradation catalyzed by TiO_(2) catalyst to demonstrate the feasibility of implementing efficient chemical protocol to fast degrading polyethylene terephthalate(PET)-FMPs(a major FMP type existing in environment).The result shows that a hydrothermal pretreatment(180℃/12 h)is necessary to induce the initial rough appearance and molecular weight reduction.With the comprehensive action of the nano-flower shaped N doped-TiO_(2) catalyst(Pt@N-TiO_(2)-1.5%)on the relatively low molecular weight intermediates,an approximate 29%weight loss was induced on the pretreated PET-FMPs,which is about 8 times superior to the untreated sample.This work not only achieves a superior degradation effect of PET-FMPs,but also provides a new inspiration for the proposal of reduction strategies in the field of environmental remediation in the future. | Dawang Zhou Hongxia Luo Fangzhou Zhang Jing Wu Jianping Yang Huaping Wang | 2022 | Advanced Fiber Materials2022，4，5： | 1 |
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