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| 1 | Transcriptomics and metabolomics analyses provide insights into postharvest ripening and senescence of tomato fruit under low temperature显示文摘Tomato is one of the most important vegetable crops in the world and is a model plant used to study the ripening of climacteric fleshy fruit.During the ripening process of tomato fruit,flavor and aroma metabolites,color,texture and plant hormones undergo significant changes.However,low temperatures delayed the ripening process of tomato fruit,inhibiting flavor compounds and ethylene production.Metabolomics and transcriptomics analyses of tomato fruit stored under low temperature(LT,5°C)and room temperature(RT,25°C)were carried out to investigate the effects of storage temperature on the physiological changes in tomato fruit after harvest.The results of transcriptomics changes revealed that the differentially expressed genes(DEGs)involved in tomato fruit ripening,including several kinds of transcription factors(TFs)(TCP,WRKY,MYB and bZIP),enzymes involved in cell wall metabolism[beta-galactosidase(β-GAL),pectinesterase(PE)and pectate lyase(PL),cellulose and cellulose synthase(CESA)],enzymes associated with fruit flavor and aroma[acetyltransferase(AT),malic enzyme(ME),lipoxygenase(LOX),aldehyde dehydrogenase(ALDH),alcohol dehydrogenase(ADH)and hexokinase(HK)],genes associated with heat stress protein 70 and genes involved in the production of plant hormones such as Ethylene responsive factor 1(ERF1),Auxin/indoleacetic acids protein(AUX/IAA),gibberellin regulated protein.Based on the above results,we constructed a regulatory network model of the effects of different temperatures during the fruit ripening process.According to the analysis of the metabolomics results,it was found that the contents of many metabolites in tomato fruit were greatly affected by storage temperature,including,organic acids(L-tartaric acid,a-hydroxyisobutyric acid and 4-acetamidobutyric acid),sugars(melezitose,beta-Dlactose,D-sedoheptulose 7-phosphate,2-deoxyribose 1-phosphate and raffinose)and phenols(coniferin,curcumin and feruloylputrescine).This study revealed the effects of storage temperature on postharvest tomato fruit and provided a basis for further understanding of the molecular biology and biochemistry of fruit ripening. | Chunmei Bai Caie Wu Lili Ma Anzhen Fu Yanyan Zheng Jiawei Han Changbao Li Shuzhi Yuan Shufang Zheng Lipu Gao Xinhua Zhang Qing Wang Demei Meng Jinhua Zuo | 2023 | Horticultural Plant Journal2023，9，1： | 3 |
| 2 | Characterization of macrolide resistance in Mycoplasma pneumoniae isolated from children in Shanghai, China显示文摘 | Yang Liu Xinyu Ye Hong Zhang Xiaogang Xu Wanhua Li Demei Zhu Minggui Wang | 2010 | Diagnostic Microbiology & Infectious Disease2010，，4： | 2 |
| 3 | The facile synthesis of hierarchical NiCoO 2 nanotubes comprised ultrathin nanosheets for supercapacitors显示文摘 | Xin Xu Han Zhou Shujiang Ding Jun Li Beibei Li Demei Yu | 2014 | Journal of Power Sources2014，，： | 1 |
| 4 | Synthesis of Coumarin-Pillar[5]arene as a Selective Fluorescent Probe for Methyl-Parathion显示文摘A novel coumarin-pillar[5]arene derivative(P5C10)was prepared.Fluorescence spectroscopy indicates that P5C10 shows good selectivity towards methyl-parathion.A combination of NMR spectra,MALDI-TOF spectra and computational calculations reveals the formation of a host-guest complex driven byπ-πstacking interactions. | Fan Zhang Xianliang Cao Demei Tian Haibing Li | 2015 | Chinese Journal of Chemistry2015，33，3： | 0 |
| 5 | Influence of the tangential velocity on the compressible Kelvin–Helmholtz instability with nonequilibrium effects显示文摘Kelvin–Helmholtz(KH)instability is a fundamental fluid instability that widely exists in nature and engineering.To better understand the dynamic process of the KH instability,the influence of the tangential velocity on the compressible KH instability is investigated by using the discrete Boltzmann method based on the nonequilibrium statistical physics.Both hydrodynamic and thermodynamic nonequilibrium(TNE)effects are probed and analyzed.It is found that,on the whole,the global density gradients,the TNE strength and area firstly increase and decrease afterwards.Both the global density gradient and heat flux intensity in the vertical direction are almost constant in the initial stage before a vortex forms.Moreover,with the increase of the tangential velocity,the KH instability evolves faster,hence the global density gradients,the TNE strength and area increase in the initial stage and achieve their peak earlier,and their maxima are higher for a larger tangential velocity.Physically,there are several competitive mechanisms in the evolution of the KH instability.(i)The physical gradients increase and the TNE effects are strengthened as the interface is elongated.The local physical gradients decrease and the local TNE intensity is weakened on account of the dissipation and/or diffusion.(ii)The global heat flux intensity is promoted when the physical gradients increase.As the contact area expands,the heat exchange is enhanced and the global heat flux intensity increases.(iii)The global TNE intensity reduces with the decreasing of physical gradients and increase with the increasing of TNE area.(iv)The nonequilibrium area increases as the fluid interface is elongated and is widened because of the dissipation and/or diffusion. | Yaofeng Li Huilin Lai Chuandong Lin Demei Li | 2022 | Frontiers of physics2022，17，6： | 0 |
| 6 | Multicenter Antimicrobial Resistance Surveillance of Clinical Isolates from Major Hospitals—China,2022显示文摘Summary What is already known about this topic?Bacterial resistance surveillance is crucial for monitoring and understanding the trends and spread of drug-resistant bacteria.What is added by this report?The number of strains collected in 2022 increased compared to 2021.The top five bacteria,including Escherichia coli,Klebsiella pneumoniae,Staphylococcus aureus,Pseudomonas aeruginosa,and Acinetobacter baumannii,remained largely unchanged.The detection rate of methicillin-resistant strains continued to decrease.Among clinical Enterobacterales isolates,the resistance rate to carbapenems was generally below 13%,except for Klebsiella spp.,which had a resistance range of 20.4%to 21.9%.Most clinical Enterobacterales isolates were highly susceptible to tigecycline,colistin,and polymyxin B,with resistance rates ranging from 0.1%to 12.6%.The detection rate of meropenem-resistant P.aeruginosa and meropenemresistant Acinetobacter baumannii showed a decreasing trend for the fourth consecutive year.What are the implications for public health practice?Multidrug-resistant bacteria remain a significant public health challenge in clinical antimicrobial treatment.To effectively address bacterial resistance,it is essential to enhance both bacterial resistance surveillance and the prudent use of antimicrobial agents. | Yan Guo Li Ding Yang Yang Renru Han Dandan Yin Shi Wu Demei Zhu Fupin Hu | 2023 | China CDC weekly2023，5，52： | 0 |
| 7 | In situ localization of BiVO_(4) onto two-dimensional MXene promoting photoelectrochemical nitrogen reduction to ammonia显示文摘The existing industrial ammonia synthesis usually adopts the Haber-Bosch process,which requires harsh conditions of high temperature and high pressure,and consumes high energy.Under this circumstance,photoelectrochemical(PEC)catalysis is regarded as a promising method for N_(2) reduction reaction(NRR),but bears problems of low efficiency and yield.Thus,exploring active catalysts remains highly desirable.In this work,BiVO_(4)@MXene hybrids have been facilely synthesized by a hydrothermal route.The heterojunctions by the in situ growth of BiVO_(4) onto two-dimensional(2D)MXene greatly increase the NRR efficiency:under photoelectric conditions,the optimized NH_(3) yield is 27.25µg h^(-1) cm^(-2),and the Faraday efficiency achieves 17.54% at−0.8 V relative to the reversible hydrogen electrode(RHE),which are higher than most state-of-the-art NRR(photo)electrocatalysts.The mechanism speculation shows the enhanced light absorption range and the heterojunction formation largely promote the separation and the transfer efficiency of photogenerated carriers,thereby improving the PEC catalytic ability.Therefore,this work provides a hybrid route to combine the advantages of photo and electric catalysis for effective artificial nitrogen fixation. | Demei Zhang Shiyu Yang Xiaoyu Fang Huifeng Li Xuebo Chen Dongpeng Yan | 2022 | Chinese Chemical Letters2022，33，10： | 0 |
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