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| 1 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 2 | TaSCL14,a Novel Wheat(Triticum aestivum L.)GRAS Gene,Regulates Plant Growth,Photosynthesis,Tolerance to Photooxidative Stress,and Senescence显示文摘Rates of photosynthesis,tolerance to photooxidative stress,and senescence are all important physiological factors that affect plant development and thus agricultural productivity.GRAS proteins play essential roles in plant growth and development as well as in plant responses to biotic and abiotic stresses.So far few GRAS genes in wheat(Triticum aestivum L.)have been characterized.A previous transcriptome analysis indicated that the expression of a GRAS gene(TaSCL14) was induced by high-light stress in Xiaoyan 54(XY54),a common wheat cultivar with strong tolerance to high-light stress.In this study,TaSCL14 gene was isolated from XY54 and mapped on chromosome 4A.TaSCL14 was expressed in various wheat organs,with high levels in stems and roots.Our results confirmed that TaSCL14 expression was indeed responsive to high-light stress.Barley stripe mosaic virus(BSMV)-based virus-induced gene silencing(VIGS)of TaSCL14 in wheat was performed to help characterize its potential functions.Silencing of TaSCL14 resulted in inhibited plant growth,decreased photosynthetic capacity,and reduced tolerance to photooxidative stress.In addition,silencing of TaSCL14 in wheat promoted leaf senescence induced by darkness.These results suggest that TaSCL14 may act as a multifunctional regulator involved in plant growth,photosynthesis,tolerance to photooxidative stress,and senescence. | Kunmei Chen Hongwei Li Yaofeng Chen Qi Zheng Bin Li Zhensheng Li | 2015 | Journal of Genetics and Genomics2015，42，1： | 11 |
| 3 | One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system显示文摘The CRISPR/Cas(clustered regularly interspaced short palindromic repeats/CRISPR-associated)adaptive immune system,which was discovered in bacteria and archaea,can specifically degrade invasive viral and plasmid DNA by base pairing between crRNAs(CRISPR RNAs)and the target DNA[1,2].Recently,the Streptococcus pyogenes type II CRISPR system was shown to be able to perform efficient targeted gene disruption by employing three fundamental components:(1)Cas9 endonuclease. | Hongbing HAN Yonghe MA Tao WANG Ling LIAN Xiuzhi TIAN Rui HU Shoulong DENG Kongpan LI Feng WANG Ning LI Guoshi LIU Yaofeng ZHAO Zhengxing LIAN | 2014 | Frontiers of Agricultural Science and Engineering2014，1，1： | 8 |
| 4 | Environmental change since mid-Pleistocene recorded in Shangshan achaeological site of Zhejiang显示文摘在迟了的时期的大约 173 ka BP 中间 -- 更新世,河的第二个平台被形成了由于因为气候,海水平变化和构造行动,高举发生在 Shangshan 区域。在 173 75 ka BP 之间。 | MAO Longjiang MO Duowen JIANG Leping JIA Yaofeng LIU Xiaoyan LI Minglin ZHOU Kunshu SHI Chenxi | 2008 | Journal of Geographical Sciences2008，18，2： | 6 |
| 5 | In situ trapped high-density single metal atoms within graphene: Iron-containing hybrids as representatives for efficient oxygen reduction显示文摘因为他们为特殊催化作用的效率和 chemoselectivity 比传统的催化剂的那些优异,原子地驱散的催化剂在精力变换应用吸引了注意。然而,他们在支持上由于极其低的装载金属的内容有限制,在在高温度的金属地点和数量以及低稳定性的精确控制的困难。我们经由葡萄糖, dicyandiamide,和无机的金属盐的单个步的热热分解准备了一个高度做的单个金属原子混血儿。高角度的环形的黑暗扫描地的传播电子显微镜学(HAADF 茎) 和 X 光检查吸收罚款结构光谱学(XAFS ) 表明做进 graphene 矩阵的氮原子为由产生 metal-Nx 协作结构的金属原子稳定是枢轴的。由于 graphene 矩阵的强壮的抛锚效果,装载内容的金属是在孤立的原子混血儿的超过 4 wt.%( 磅内容象在做磅的混血儿的 9.26 wt.% 一样高) 。而且,单个做铁的混血儿(做 Fe@N 的 graphene ) 为氧减小反应显示出显著 electrocatalytic 表演。山峰力量密度是 199 | Daobin Liu Chuanqiang Wu Shuangming Chen Shiqing Ding Yaofeng Xie Changda Wang Tao Wang Yasir A. Haleem Zia ur Rehman Yuan Sang Qin Liu Xusheng Zheng Yu Wang Binghui Ge Hangxun Xu Li Song | 2018 | Nano Research2018，11，4： | 6 |
| 6 | Comparative study of estimation methods for genomic breeding values显示文摘Estimation of genomic breeding values is important in genomic selection. Bayesian and BLUP methods are the main techniques employed. In this study,we conducted a comparative study of Bayes A, Bayes B,Bayes Cp and GBLUP methods in simulated data and real data of Chinese Holstein cattle. Results showed that, in simulated data, the accuracies of all methods were all similarly elevated with the increase of reference population size, but they made different responses to the changes of marker number or QTL number. In real data of Chinese Holstein cattle, Bayes A generated the highest accuracy almost for all six traits, and GBLUP performed as well as Bayes A for the traits of milk yield, fat yield and protein yield, while for the trait of fat percentage, protein percentage and somatic cell score, three Bayesian methods showed superior to GBLUP. Comprehensively analyzing above results, it can be speculated that accuracies of the three Bayesian methods are not only influenced by the absolute value of QTL number or marker number, but may also be influenced by the ratio of QTL number to marker number. And there is at least one kind of Bayesian methods performing better than GBLUP, when the ratio of QTL number versus marker number is very small or involving large-effect QTL. | Chonglong Wang Qin Zhang Li Jiang Rong Qian Xiangdong Ding Yaofeng Zhao | 2016 | Science Bulletin2016，61，5： | 3 |
| 7 | FSHB subunit gene is associated with major gene controlling litter size in commercial pig breeds显示文摘 | Yaofeng Zhao Ning Li Lu Xiao Gengsheng Cao Yizhen Chen Shun Zhang Yongfu Chen Changxin Wu Jiansheng Zhang Shiquan Sun Xueqing Xu | 1998 | Science in China Series C: Life Sciences1998，，6： | 1 |
| 8 | End End-to to-End Chinese Entity Recognition Based on BERT BERT-BiLSTM BiLSTM-ATT ATT-CRF显示文摘Traditional named entity recognition methods need professional domain knowl-edge and a large amount of human participation to extract features,as well as the Chinese named entity recognition method based on a neural network model,which brings the prob-lem that vector representation is too singular in the process of character vector representa-tion.To solve the above problem,we propose a Chinese named entity recognition method based on the BERT-BiLSTM-ATT-CRF model.Firstly,we use the bidirectional encoder representations from transformers(BERT)pre-training language model to obtain the se-mantic vector of the word according to the context information of the word;Secondly,the word vectors trained by BERT are input into the bidirectional long-term and short-term memory network embedded with attention mechanism(BiLSTM-ATT)to capture the most important semantic information in the sentence;Finally,the conditional random field(CRF)is used to learn the dependence between adjacent tags to obtain the global optimal sentence level tag sequence.The experimental results show that the proposed model achieves state-of-the-art performance on both Microsoft Research Asia(MSRA)corpus and people’s daily corpus,with F1 values of 94.77% and 95.97% respectively. | LI Daiyi TU Yaofeng ZHOU Xiangsheng ZHANG Yangming MA Zongmin | 2022 | ZTE Communications2022，20，S01： | 1 |
| 9 | Toward low-sensitive and high-energetic cocrystal 1: evaluation of the power and the safety of observed energetic cocrystals显示文摘 | Zhang Chaoyang Cao Yaofeng Li Hongzhen | 2013 | CrystEngComm2013，15，： | 1 |
| 10 | Are Sc-C and Sc-P Bonds Reactive in Scandium Phosphinoalkylidene Complex? Insights on a Versatile Reactivity显示文摘 | Weiqing Mao Li Xiang Carlos Alvarez Lamsfus Laurent Maron Xuebing Leng Yaofeng Chen | 2018 | Chinese Journal of Chemistry2018，36，10： | 1 |
| 11 | A rice microsomal delta-12 fatty acid desaturase can enhance resistance to cold stress in yeast and Oryza sativa显示文摘 | Jinlei Shi Yingping Cao Xiaorong Fan Min Li Yaofeng Wang Feng Ming | 2012 | Molecular Breeding2012，，3： | 1 |
| 12 | Divalent Ytterbium Iodide Supported by β-Diketiminato Based Tridentate Ligand: Synthesis, Structure and Small Molecule Activation显示文摘Divalent ytterbium lodide[LYb(μ-I)(THF)]2(1;L=[MeC(NDIPP)CHC(Me)NCH2CH2NMe2]^-,DIPP=2,6-(iPr)2C6H3)was synthesized and its reactivity was studied.Complex 1 was synthesized by salt metathesis of Ybl2(THF)2 with the potassium salt of ligand(KL)in high yield.In the reactions with trimethylsil azide,azobenzene,sulfur and diphenyl disufide,complex 1 acts as a 2e reductant.In the reaction with CO2,the central carbon atom ofβ-diketiminato backbone in 1 nuclephilcally attacks the CO2 molecule to give a divalent ytterbium carboxylate. | Xiaojuan Liu Li Xiang Chen Wang Bingwu Wang Xuebing Leng Yaofeng Chen | 2020 | Chinese Journal of Chemistry2020，38，3： | 1 |
| 13 | Construction of nonsingular formulae of variance and covariance function of disturbing gravity gradient tensors显示文摘When the computational point is approaching the poles,the variance and covariance formulae of the disturbing gravity gradient tensors tend to be infinite,and this is a singular problem.In order to solve the problem,the authors deduced the practical non-singular computational formulae of the first-and second-order derivatives of the Legendre functions and two kinds of spherical harmonic functions,and then constructed the nonsingular formulae of variance and covariance function of disturbing gravity gradient tensors. | Liu Xiaogang Zhang Yaofeng Li Yan Xu Kang | 2013 | Geodesy and Geodynamics2013，4，4： | 1 |
| 14 | Scandium terminal imido complex induced intramolecular C-N bond cleavage and transformation显示文摘The scandium terminal imido complex supported by a monoanionic tetradentate NNNN ligand, [LSc=N(DIPP)](L = [MeC(N(DIPP))CHC(Me)(NCH2CH2N(Me)CH2CH2NMe2]-, DIPP = 2,6-(iPr)2C6H3)(1), undergoes a C–N bond cleavage at elevated temperature to give a mononuclear scandium anilido intermediate 2a, which subsequently aggregates into a binuclear scandium anilido complex 2. The mononuclear intermediate 2a reacts with alkyne or imine to provide two scandium anilido complexes 3 and 4, which contain a dianionic tetradentate NNNC ligand or a dianionic tetradentate NNNN ligand. DFT calculations on the reaction mechanism of C–N bond cleavage in 1 were also performed. | CHU JiaXiang ZHOU QingHai LI YuXue LENG XueBing CHEN YaoFeng | 2014 | Science China Chemistry2014，57，8： | 1 |
| 15 | Exploring the stage-specific roles of Tcf-1 in T cell development and malignancy at single-cell resolution显示文摘Tcf-1(encoded by Tcf7)not only plays critical roles in promoting T cell development and differentiation but also has been identified as a tumor suppressor involved in preventing T cell malignancy.However,the comprehensive mechanisms of Tcf-1 involved in T cell transformation remain poorly understood.In this study,Tcf7^(fl/fl)mice were crossed with Vav-cre,Lck-cre,or Cd4-cre mice to delete Tcf-1 conditionally at the beginning of the HSC,DN2-DN3,or DP stage,respectively.The defective T cell development phenotypes became gradually less severe as the deletion stage became more advanced in distinct mouse models.Interestingly,consistent with Tcf7^(-/-)mice,Tcf^(fl/fl)Vav-cre mice developed aggressive T cell lymphoma within 45 weeks,but no tumors were generated in Tcf7^(fl/fl)Lck-cre or Tcf^(fl/fl)CdA-cre mice.Single-cell RNA-seq(ScRNA-seq)indicated that ablation of Tcf-1 at distinct phases can subdivide DN1 cells into three clusters(C1,C2,and C3)and DN2-DN3 cells into three clusters(C4,C5,and C6).Moreover,Tcf-1 deficiency redirects bifurcation among divergent cell fates,and clusters C1 and C4 exhibit high potential for leukemic transformation.Mechanistically,we found that Tcf-1 directly binds and mediates chromatin accessibility for both typical T cell regulators and proto-oncogenes#including Myb,Mycn,Runxl,and Lyl1 in the DN1 phase and Lefb ld2,Dtxb Fyn,Bclllb,and Zfp36l2 in the DN2-DN3 phase.The aberrant expression of these genes due to Tcf-1 deficiency in very early T cells contributes to subsequent tumorigenesis.Thus,we demonstrated that Tcf-1 plays stage-specific roles in regulating early thymocyte development and transformation,providing new insights and evidence for clinical trials on T-ALL leukemia. | Fang Wang Zhihong Qi Yingpengao Guotao Yu Tao Feng Tianyan Zhao Hai-Hui Xue Yaofeng Zhao Peng Jiang Li Bao Shuyang Yu | 2021 | Cellular & Molecular Immunology2021，18，3： | 0 |
| 16 | Genome‑wide DNA methylation and transcriptome analyses reveal the key gene for wool type variation in sheep显示文摘Background Wool fibers are valuable materials for textile industry.Typical wool fibers are divided into medullated and non-medullated types,with the former generated from primary wool follicles and the latter by either primary or secondary wool follicles.The medullated wool is a common wool type in the ancestors of fine wool sheep before breeding.The fine wool sheep have a non-medullated coat.However,the critical period determining the type of wool follicles is the embryonic stage,which limits the phenotypic observation and variant contrast,making both selection and studies of wool type variation fairly difficult.Results During the breeding of a modern fine(MF)wool sheep population with multiple-ovulation and embryo transfer technique,we serendipitously discovered lambs with ancestral-like coarse(ALC)wool.Whole-genome rese-quencing confirmed ALC wool lambs as a variant type from the MF wool population.We mapped the significantly associated methylation locus on chromosome 4 by using whole genome bisulfite sequencing signals,and in turn identified the SOSTDC1 gene as exons hypermethylated in ALC wool lambs compare to their half/full sibling MF wool lambs.Transcriptome sequencing found that SOSTDC1 was expressed dozens of times more in ALC wool lamb skin than that of MF and was at the top of all differentially expressed genes.An analogy with the transcriptome of coarse/fine wool breeds revealed that differentially expressed genes and enriched pathways at postnatal lamb stage in ALC/MF were highly similar to those at the embryonic stage in the former.Further experiments validated that the SOSTDC1 gene was specifically highly expressed in the nucleus of the dermal papilla of primary wool follicles.Conclusion In this study,we conducted genome-wide differential methylation site association analysis on differen-tial wool type trait,and located the only CpG locus that strongly associated with primary wool follicle development.Combined with transcriptome analysis,SOSTDC1 was identified as the only gene at this locus that was specifically overexpressed in the primary wool follicle stem cells of ALC wool lamb skin.The discovery of this key gene and its epigenetic regulation contributes to understanding the domestication and breeding of fine wool sheep. | Jiankui Wang Guoying Hua Ganxian Cai Yuhao Ma Xue Yang Letian Zhang Rui Li Jianbin Liu Qing Ma Keliang Wu Yaofeng Zhao Xuemei Deng | 2023 | Journal of Animal Science and Biotechnology2023，14，6： | 0 |
| 17 | Immunoglobulin genes and diversity: what we have learned from domestic animals显示文摘This review focuses on the diversity of immunoglobulin (Ig) genes and Ig isotypes that are expressed in domestic animals. Four livestock species-cattle, sheep, pigs, and horses-express a full range of Ig heavy chains (IgHs), including μ, δ, γ, ε, and α. Two poultry species (chickens and ducks) express three IgH isotypes, μ, υ, and α, but not δ. The κ and λ light chains are both utilized in the four livestock species, but only the λ chain is expressed in poultry. V(D)J recombination, somatic hypermutation (SHM), and gene conversion (GC) are three distinct mechanisms by which immunoglobulin variable region diversity is generated. Different domestic animals may use distinct means to diversify rearranged variable regions of Ig genes. | Yi Sun Zhancai Liu Liming Ren Zhiguo Wei Ping Wang Ning Li Yaofeng Zhao | 2012 | Journal of Animal Science and Biotechnology2012，3，4： | 0 |
| 18 | Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330显示文摘Lactoferrin is a member of the transferrin family of multifunctional iron binding glycoproteins.While numerous physiological functions have been described for lactoferrin,the mechanisms underlying these functions are not clear.To further study the functions and mechanisms of lactoferrin,we modified the lactoferrin promoter of mice using the CRISPR/Cas9 system to reduce or eliminate lactoferrin expression.Seven mice with lactoferrin promoter mutations were obtained with an efficiency of 24%(7/29)by injecting the plasmid pX330,expressing a small guide RNA and human codonoptimized SpCas9,into fertilized eggs of mice.Plasmid integration and off-targeting of pX330 were not detected.These results confirmed that pronuclear injection of a circular plasmid is a feasible and efficient method for targeted mutagenesis in mice. | Mengxu GE Fei LIU Fei CHANG Zhaolin SUN Jing FEI Ying GUO Yunping DAI Zhengquan YU Yaofeng ZHAO Ning LI Qingyong MENG | 2015 | Frontiers of Agricultural Science and Engineering2015，2，3： | 0 |
| 19 | Nanoscale storage encryption: data storage in synthetic DNA using a cryptosystem with a neural network显示文摘Dear Editor,Synthetic DNA is emerging as a new data storage medium with high density and durable preservation capability potential(Ceze et al., 2019;Yazdi et al., 2015), thus enhancing the security of data stored in DNA is particularly important(Cherian et al., 2013;El-Zoghabi et al., 2013;Kalsi et al.,2018). | Yue Teng Shan Yang Liyan Liu Ruicun Liu Yaofeng Chen Jinyu Li Qian Yang Tuoyu Liu Yujun Cui Peng Cheng Shengqi Wang | 2022 | Science China(Life Sciences)2022，65，8： | 0 |
| 20 | Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice显示文摘Butyrylcholinesterase(BCHE)is a natural bioscavenger that protects humans against organophosphate toxicity.Due to the limited yield of human BCHE(hBCHE)when purifying from human plasma,it is necessary to find an alternative method to produce this protein.One potential method is to produce transgenic livestock that make modified milk containing high concentration of hBCHE.In this study,we cloned the hBCHEgene into a human lactoferrin(hLF)bacterial artificial chromosome(BAC)construct to make a hLFhBCHE BAC construct.Subsequently,we injected the BAC construct into pronuclei of mouse fertilized embryos and generated transgenic mice.Expression analysis showed that recombinant hBCHE(rhBCHE)was expressed efficiently in the mammary gland of the transgenic mice and the concentration of rhBCHE in the milk of individual mice ranged from 7612 to 15928 mg·L^(–1).Protein function tests showed that rhBCHE has the same enzymatic activity as the native hBCHE.Our results pave the way for making transgenic livestock to produce large quantities of rhBCHE. | Dan LU Shengzhe SHANG Shen LIU Ying WU Fangfang WU Tan TAN Qiuyan LI Yunping DAI Xiaoxiang HU Yaofeng ZHAO Ning LI | 2014 | Frontiers of Agricultural Science and Engineering2014，1，3： | 0 |
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