|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effects of cotransplantated Schwann cells and neural stem cells in a rat model of Alzheimer's disease显示文摘Schwann cells (SCs) are significantly better at promoting neural stem cell (NSCs) proliferation, differentiation and synaptic formation when cocultured with NSCs in vitro, compared with cultured in a single nerve growth factor. The present study transplanted NSCs and SCs into the brain of a rat model of Alzheimer's disease to investigate the effect of cotransplantation. Results show transplantation of both NSCs alone and NSCs + SCs significantly promoted learning and memory functions in Alzheimer's disease rats, decreased glial fibrillary acidic protein and calcium binding protein S100β expression, but increased expression of the cholinergic neuron marker choline acetyl transferase mRNA. The effect of NSCs + SCs cotransplantation was, however, more significant. NSCs and SCs cotransplantation significantly reduced the number of astrocytes and increased cholinergic neurons, facilitating the recovery of learning and memory function, compared with NSCs transplantation alone. | Yan Zhan Dihui Ma Yu Zhang | 2011 | Neural Regeneration Research2011，6，4： | 13 |
| 2 | Protective effects of transplanted neural stem cells on the brain of Alzheimer’s disease rats显示文摘BACKGROUND: To date, no drugs are able to halt the progression of Alzheimer's disease (AD). Neural stem cells (NSCs) transplantation has been widely used to treat AD, but the mechanism of AD treatment remains unclear. OBJECTIVE: To observe changes in protein and factors in the hippocampus and frontal lobe of AD rats following NSCs transplantation, and to understand mechanism of action of NSCs transplantation in AD treatment. DESIGN, TIME AND SETTING: A randomized, controlled animal study was conducted at the First Clinical Hospital, Jilin University, China from July 2007 to March 2009. MATERIALS: NSCs were harvested from the hippocampus of 10 E16 Wistar rats. METHODS: A total of 57 male adult Wistar rats were equally and randomly divided into normal control, AD model and NSCs groups. AD models were established in the AD model and NSCs groups by bilateral removal of hippocampus. At 2 weeks postsurgery, NSCs were transplanted into the hippocampus of rats from the NSCs group. MAIN OUTCOME MEASURES: Protein levels were measured in the hippocampus of rats from normal control, NSCs and AD model groups using proteomics. Expression of choline acetyl transferase mRNA, glial fibrillary acidic protein and S100β was measured in the hippocampus and frontal lobe of rats using in situ hybridization and immunohistochemistry. RESULTS: Expression of choline acetyl transferase mRNA, heat shock protein 70, heat shock protein 90, F-actin and actin was significantly higher in the NSCs group compared with AD model group. Glial fibrillary acidic protein and S100β expression was less in the NSCs group compared with AD model group. CONCLUSIOIN: NSCs implanted into the brain may generate new neural cells, which can relieve damage to the cholinergic system and resist apoptosis. NSCs transplantation plays a protective role in the cholinergic system in the AD rats to some extent. | Yan Zhan Dihui Ma Yu Zhang Ming Chang Linsen Hu | 2010 | Neural Regeneration Research2010，5，11： | 9 |
| 3 | Midbrain enkephalin expression in a rat migraine model following intragastric scorpion powder administration显示文摘Scorpion has strong analgesic effects,but its analgesic mechanisms remain unclear.This study investigated the effects of scorpion powder on enkephalin expression in the midbrain of rats with nitroglycerin-induced migraine at mRNA and protein levels.Results confirmed that migraine rat abnormal behavior was significantly improved,and proenkephalin mRNA expression was significantly increased following treatment with scorpion.The number of methionine-enkephalin-positive cells in the migraine rats following treatment with scorpion was significantly increased,but no significant difference in the number of leucine-enkephalin-positive cells was detectable compared with migraine and normal rats.Taken together,these results show that scorpion exerts potentially beneficial effects by promoting enkephalin expression in nitroglycerin-induced migraine rats. | Gang Yao Xiangdan Luo Dihui Ma Tingmin Yu | 2011 | Neural Regeneration Research2011，6，12： | 9 |
| 4 | Effects of Toutongning capsule on enkephalin expression in a rat migraine headache model显示文摘Toutongning capsule is used for the treatment of migraine headaches,and has yielded therapeutically beneficial effects.However,whether Toutongning capsule exerts its effects via endogenous opioid peptides remains poorly understood.This study investigated the effects of Toutongning capsule on enkephalin expression in the midbrain of rats with nitroglycerin-induced migraine headache at the mRNA and protein levels.Results confirmed that proenkephalin mRNA levels were significantly upregulated following treatment with Toutongning capsule.The numbers of methionine-enkephalin and leucine-enkephalin-positive cells were significantly greater in the migraine headache rats following treatment with Toutongning capsule compared with the model group.Taken together,these results show that Toutongning capsule exerts potentially beneficial effects by promoting enkephalin expression in nitroglycerin-induced migraine headache rats. | Gang Yao Yuhong Man Jingjing Qi Tingmin Yu Dihui Ma | 2011 | Neural Regeneration Research2011，6，9： | 7 |
| 5 | Effects of glutamate and nimodipine on survival rate of embryonic rat neuronal stem cells cultured in vitro显示文摘BACKGROUND: At least three types of calcium ion channel (T, N, and L) have been recognized in nerve cells, but only the L type of channel is sensitive to drugs. Theoretically, nimodipine can lead to L-type channel inactivation and prevent calcium ion inflow, thereby exhibiting protective effects on nerve cells. OBJECTIVE: To observe the protective effects of nimodipine on glutamate-induced injury to embryonic rat neural stem cells, and to make a comparison with MK-801, a nonselective glutamate receptor antagonist. DESIGN, TIME AND SETTING: The present in vitro experiment pertaining to neural stem cells was performed at the Department of Neurology, First Hospital, Jilin University between January 2005 and December 2006. MATERIALS: Glutamate was sourced from the Shanghai Biological Research Institute of the Chinese Academy of Sciences. Nimodipine was provided by Bayer Company, Germany. Brain tissue was taken from Wistar rats on day 15 of gestation for isolation and culture of neural stem cells. METHODS: Passage 2 neural stem cell spheres were taken for preparation of single cell suspension. The prepared single cell suspension was divided into 4 groups: (1) Normal control, normally cultured. (2) Glutamate, cultured with 50, 100, 200, 500, and 1 000 μmol/L glutamate. (3) Nimodipine, received a 30- minute nimodipine [1×(10-8-10-2) g/L] culture followed by a glutamate (200 μmol/L) treatment step. (4) MK-801, given as 30- minute MK-801 (100 μmol/L) culture, followed by a glutamate (200 μmol/L) treatment step. MAIN OUTCOME MEASURES: Determination of glutamate-induced cell death by methyl thiazolyl tetrazolium (MTT) assay; calculation of neural stem cell survival rate following addition of nimodipine. RESULTS: The survival rate of neural stem cells was approximately 25.26% following 24 hour 50 μmol/L glutamate culture and gradually decreased as the glutamate dose increased (P < 0.05-0.01). Only 9.27% of neural stem cells survived when the glutamate dose was 1 000 μmol/L. The survival rate of neural stem cells was significantly higher, in a dose-dependent manner, in the nimodipine [1×(10-7-10-2) g/L] group than in the glutamate group. In addition, the MK-801 group exhibited a higher survival rate after 24 hour treatment than the glutamate group (P < 0.01). CONCLUSION: Glutamate (50-1 000 μmol/L) induces injury to neural stem cells dose-dependently. Nimodipine exhibits protective effects on injury to neural stem cells and presents the effects in a dose-dependent manner at 1×(10-7-10-2) g/L. Nimodipine displays neuroprotective effects equivalent to MK-801. | Xiaohong Lu Hailong Fu Qiang Sun Li Cui Dihui Ma Weihong Lin | 2008 | Neural Regeneration Research2008，3，12： | 0 |
| 6 | Calcium ion channel and epilepsy显示文摘OBJECTIVE: To review the relationship between calcium ion channel and epilepsy for well investigating the pathogenesis of epilepsy and probing into the new therapeutic pathway of epilepsy. DATA SOURCES: A computer-based online research Calcium ion channel and epilepsy related articles published between January 1994 and December 2006 in the CKNI and Wanfang database with the key words of 'calcium influxion, epilepsy, calcium-channel blocker'. The language was limited to Chinese. At the same time, related articles published between January 1993 and December 2006 in Pubmed were searched for on online with the key words of 'calcium influxion, epilepsy' in English. STUDY SELECTION: The materials were selected firstly. Inclusive criteria: ① Studies related to calcium ion channel and the pathogenesis of epilepsy. ② Studies on the application of calcium ion channel blocker in the treatment of epilepsy. Exclusive criteria: repetitive or irrelated studies. DATA EXTRACTION: According to the criteria, 123 articles were retrieved and 93 were excluded due to repetitive or irrelated studies. Altogether 30 articles met the inclusive criteria, 11 of them were about the structure and characters of calcium ion channel, 10 about calcium ion channel and the pathogenesis of epilepsy and 9 about calcium blocker and the treatment of epilepsy. DATA SYNTHESIS: Calcium ion channels mainly consist of voltage dependent calcium channel and receptor operated calcium channel. Depolarization caused by voltage gating channel-induced influxion is the pathological basis of epileptic attack, and it is found in many studies that many anti-epileptic drugs have potential and direct effect to rivalizing voltage-dependent calcium ion channel. CONCLUSION: Calcium influxion plays an important role in the seizure of epilepsy. Some calcium antagonists seen commonly are being tried in the clinical therapy of epilepsy that is being explored, not applied in clinical practice. If there are enough evidences to justify its effect in epilepsy, there will be considerable clinical significance. | Yudan Lue Weihong Lin Dihui Ma | 2006 | Neural Regeneration Research2006，1，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员