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| 1 | Foveated glasses-free 3D display with ultrawide field of view via a large-scale 2D-metagrating complex显示文摘Glasses-free three-dimensional(3D)displays are one of the game-changing technologies that will redefine the display industry in portable electronic devices.However,because of the limited resolution in state-of-the-art display panels,current 3D displays suffer from a critical trade-off among the spatial resolution,angular resolution,and viewing angle.Inspired by the so-called spatially variant resolution imaging found in vertebrate eyes,we propose 3D display with spatially variant information density.Stereoscopic experiences with smooth motion parallax are maintained at the central view,while the viewing angle is enlarged at the periphery view.It is enabled by a large-scale 2D-metagrating complex to manipulate dot/linear/rectangular hybrid shaped views.Furthermore,a video rate full-color 3D display with an unprecedented 160°horizontal viewing angle is demonstrated.With thin and light form factors,the proposed 3D system can be integrated with off-the-shelf purchased flat panels,making it promising for applications in portable electronics. | Jianyu Hua Erkai Hua Fengbin Zhou Jiacheng Shi Chinhua Wang Huigao Duan Yueqiang Hu Wen Qiao Linsen Chen | 2021 | Light(Science & Applications)2021，10，11： | 14 |
| 2 | Protective effects of transplanted neural stem cells on the brain of Alzheimer’s disease rats显示文摘BACKGROUND: To date, no drugs are able to halt the progression of Alzheimer's disease (AD). Neural stem cells (NSCs) transplantation has been widely used to treat AD, but the mechanism of AD treatment remains unclear. OBJECTIVE: To observe changes in protein and factors in the hippocampus and frontal lobe of AD rats following NSCs transplantation, and to understand mechanism of action of NSCs transplantation in AD treatment. DESIGN, TIME AND SETTING: A randomized, controlled animal study was conducted at the First Clinical Hospital, Jilin University, China from July 2007 to March 2009. MATERIALS: NSCs were harvested from the hippocampus of 10 E16 Wistar rats. METHODS: A total of 57 male adult Wistar rats were equally and randomly divided into normal control, AD model and NSCs groups. AD models were established in the AD model and NSCs groups by bilateral removal of hippocampus. At 2 weeks postsurgery, NSCs were transplanted into the hippocampus of rats from the NSCs group. MAIN OUTCOME MEASURES: Protein levels were measured in the hippocampus of rats from normal control, NSCs and AD model groups using proteomics. Expression of choline acetyl transferase mRNA, glial fibrillary acidic protein and S100β was measured in the hippocampus and frontal lobe of rats using in situ hybridization and immunohistochemistry. RESULTS: Expression of choline acetyl transferase mRNA, heat shock protein 70, heat shock protein 90, F-actin and actin was significantly higher in the NSCs group compared with AD model group. Glial fibrillary acidic protein and S100β expression was less in the NSCs group compared with AD model group. CONCLUSIOIN: NSCs implanted into the brain may generate new neural cells, which can relieve damage to the cholinergic system and resist apoptosis. NSCs transplantation plays a protective role in the cholinergic system in the AD rats to some extent. | Yan Zhan Dihui Ma Yu Zhang Ming Chang Linsen Hu | 2010 | Neural Regeneration Research2010，5，11： | 9 |
| 3 | Chaperone proteins identified from synthetic proteasome inhibitor-induced inclusions in PC12 cells by proteomic analysis显示文摘女伴蛋白质在 Lewy 身体是重要的,但是女伴蛋白质的侧面不完全地被解开。Proteomic 分析被用来为进一步的学习决定蛋白质候选人。这里,从导致代理人的包括识别潜在的女伴蛋白质,我们执行了包括在为 48 h 暴露于 10 M 希腊语的第二十三个字母的 PC12 房间形成了的导致的人工地合成的 proteasome 禁止者(希腊语的第二十三个字母) 的 proteomic 分析。用通过指纹对多重蛋白质数据库寻找了的肽质量的生物化学的分别, 2-D 电气泳动,和鉴定,我们反复从导致希腊语的第二十三个字母的包括识别了八可再现的女伴蛋白质。这些, 58 kDa 葡萄糖调整了蛋白质, 75 kDa 葡萄糖调整了蛋白质,并且钙绑定蛋白质 1 是最新识别了。其它五被报导了是 Lewy 身体的一致部件。这些调查结果建议三潜在的女伴蛋白质可能在 proteasome 抑制下面在 PC12 房间被招募到导致希腊语的第二十三个字母的包括。 | Xing'an Li Yingjiu Zhang Yihong Hu Ming Chang Tao Liu Danping Wang Yu Zhang Lei Zhang Linsen Hu | 2008 | Acta Biochimica et Biophysica Sinica2008，40，5： | 4 |
| 4 | Mechanisms of rotenone-induced neurotoxicity in PC 12 cells显示文摘BACKGROUND: Rotenone-induced neurotoxicity in PC 12 cells has been widely used to study the pathogenesis of Parkinson's disease. However, the precise mechanisms underlying rotenone-induced dopaminergic neuronal degeneration in Parkinson's disease remains unclear. OBJECTIVE: To establish rotenone-induced neurotoxicity in PC 12 cells, and to investigate the possible action pathways to rotenone-induced neural cell injury at the protein level. DESIGN, TIME AND SETTING: A controlled proteomics study was performed at the Department of Neurology, First Hospital, Jilin University between March 2006 and March 2007. MATERIALS: PC 12 cells were obtained from Shanghai Cell Bank of Chinese Academy of Sciences, China. Rotenone was provided by Sigma, USA. METHODS: PC 12 cells in logarithmic growth phase were treated under experimental and control conditions, respectively. A total of 0.5 μmol/L rotenone, or the same amount of Dulbecco’s modified eagle’s medium (DMEM), was added in the experimental and control conditions, respectively. MAIN OUTCOME MEASURES: Following 72 hours of rotenone treatment, cellular survival rate was determined by methyl thiazolyl tetrazolium assay, and apoptotic changes were detected by Hoechst 33342 staining. Total cellular protein was extracted to acquire differential protein expression data utilizing two-dimensional differential in-gel electrophoresis. To identify differential protein spots, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) was used. RESULTS: In the MTT assay, the experimental condition induced significantly less cell survival compared to the control condition (P < 0.01). Hoechst 33342 staining revealed a larger number of apoptotic cells under the experimental condition compared to the control condition (P < 0.01), as determined by the presence of nuclear condensation, pyknosis, and nuclear fragmentation. Two-dimensional electrophoresis results showed that the differential expression of protein spots 1069 and 1538 was increased by 144% and 124%, respectively, while that of protein spot 1094 was decreased by 123% in the experimental condition compared to the control condition (P < 0.01). By MALDI-TOF-MS analysis and database retrieval, γ-enolase, triosephosphate isomerase 1, and eukaryotic translation initiation factor 4A were confirmed to be involved in rotenone-induced neural cell injury. CONCLUSION: γ-enolase, triosephosphate isomerase 1, and eukaryotic translation initiation factor 4A might participate in rotenone-induced neurotoxicity in PC 12 cells. | Wei Han Lizhong Sun Jiafeng Chen Ming Chang Hongyan Huo Linsen Hu | 2008 | Neural Regeneration Research2008，3，12： | 1 |
| 5 | Engineering Multi‑field‑coupled Synergistic Ion Transport System Based on the Heterogeneous Nanofluidic Membrane for High‑Efficient Lithium Extraction显示文摘The global carbon neutrality strategy brings a wave of rechargeable lithium‐ion batteries technique development and induces an ever-growing consumption and demand for lithium(Li).Among all the Li exploitation,extracting Li from spent LIBs would be a strategic and perspective approach,especially with the low energy consumption and eco-friendly membrane separation method.However,current membrane separation systems mainly focus on monotonous membrane design and structure optimization,and rarely further consider the coordination of inherent structure and applied external field,resulting in limited ion transport.Here,we propose a heterogeneous nanofluidic membrane as a platform for coupling multi-external fields(i.e.,lightinduced heat,electrical,and concentration gradient fields)to construct the multi-field-coupled synergistic ion transport system(MSITS)for Li-ion extraction from spent LIBs.The Li flux of the MSITS reaches 367.4 mmol m^(−2)h^(−1),even higher than the sum flux of those applied individual fields,reflecting synergistic enhancement for ion transport of the multi-field-coupled effect.Benefiting from the adaptation of membrane structure and multi-external fields,the proposed system exhibits ultrahigh selectivity with a Li^(+)/Co^(2+)factor of 216,412,outperforming previous reports.MSITS based on nanofluidic membrane proves to be a promising ion transport strategy,as it could accelerate ion transmembrane transport and alleviate the ion concentration polarization effect.This work demonstrated a collaborative system equipped with an optimized membrane for high-efficient Li extraction,providing an expanded strategy to investigate the other membrane-based applications of their common similarities in core concepts. | Lin Fu Yuhao Hu Xiangbin Lin Qingchen Wang Linsen Yang Weiwen Xin Shengyang Zhou Yongchao Qian Xiang‑Yu Kong Lei Jiang Liping Wen | 2023 | Nano-Micro Letters2023，15，8： | 1 |
| 6 | Two potentially specific but relevant patterns of proteomic change Response of SH-SY5Y cells to differentiation with retinoic acid followed by phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate, and susceptibility of differentiated cells to dopamine显示文摘Dopamine (DA) exposure at a dose of 100 μmol/L for 24 hours causes oxidative stress in SH-SY5Y cells with induction of neuronal differentiation by retinoic acid (RA,10 μmol/L, 72 hours) followed by phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA, 80 nmol/L, 72 hours). However, it remains unclear whether the alteration of phenotype observed in response to oxidative stress is associated with protein regulation in this cellular model for Parkinson's disease. The present study detected protein regulation affected by oxidative stress at a proteomic level: selection of differentially altered proteins using two dimensional difference in-gel electrophoresis and identification of these proteins using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. The results demonstrated significant alterations in expression of six proteins in SH-SY5Y cells following the differentiation and fourteen proteins in the differentiated cells following the exposure, exemplified by an increase of tubulin alpha1 in the former but a decrease of tubulin alpha-ubiquitous chain in the latter. These results suggest that two potentially specific but relevant patterns of proteomic change may be produced in SH-SY5Y cells with the induction of differentiation by RA followed by TPA, and in the differentiated cells after DA exposure. | Mingxiu Tian Xing'an Li Ming Chang Yingjiu Zhang Danping Wang Hongrong Xie Linsen Hu | 2010 | Neural Regeneration Research2010，5，20： | 0 |
| 7 | Identification of differentially expressed proteins in SH-SY5Y cells treated with resveratrol显示文摘To gain insight into the molecular mechanisms of resveratrol-mediated neuroprotection, two-dimensional difference gel electrophoresis in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to identify proteins differentially-expressed in SH-SY5Y cells treated with resveratrol. Compared with the control group, resveratrol treatment significantly affected the expression of four proteins: endoplasmic reticulum oxidoreductin 1-like protein alpha, p21-activated kinase 1, Archain 1, and T cell receptor beta chain. The former three were downregulated and the latter was upregulated. These proteins are primarily associated with endoplasmic reticulum stress, intracellular trafficking, and immune function. | Ying Wang Zhong Dong Hongyan Fan Ming Chang Guoyi Li Linsen Hu | 2011 | Neural Regeneration Research2011，6，21： | 0 |
| 8 | Oxidative modification of the molecular chaperone family in a PC12 cell model of Parkinson's disease induced by Z-Ile-Glu(OtBu)-Ala-Leucinal显示文摘Previous studies have demonstrated that ubiquitin-proteasome system function is significantly decreased in the substantia nigra of Parkinson's disease patients.In the present study,proteasome inhibitor Z-Ile-Glu(OtBu)-Ala-Leucinal(PSI)was used to inhibit the function of the ubiquitin-proteasome system in PC12 cells to simulate Parkinson's disease.Oxidatively modified proteins were identified to determine pathogenesis of Parkinson's disease.Results demonstrated that 24 hours of 10μmol/L PSI-treatment in PC12 cells simulated pathological characteristics of Parkinson's disease:neuronal degeneration and eosinophilic inclusion formation in neurons.In PSI-treated PC12 cells,three oxidative proteins and a molecular chaperone family member were detected:chaperonin containing t-complex polypeptide 1 subunit 3,glucose-regulated protein 58,and heat shock protein 70.This is the first study to demonstrate oxidative modification of a molecule family in a cell model of Parkinson's disease induced with PSI. | Ying Zhang Yimin Yang Jing Bai Ming Chang Linsen Hu | 2011 | Neural Regeneration Research2011，6，2： | 0 |
| 9 | Alpha-tubulin deacetylase as a potential and novel target for the prevention of Parkinson's disease progression显示文摘Parkinson's disease(PD)is the second most common neurodegenerative disorder and is characterized by its progressive course.The current therapies are aimed at alleviating symptoms by rescuing the unbalanced physiological dopamine metabolism and recovery of damaged neuronal circuits.However,these strategies result in insufficient clinical benefits for many patients and fail to halt disease progression.Therefore,new therapeutic targets could serve as the gateway against PD degeneration.One pathological hallmark of PD is the formation of intracytoplasmic protein inclusions or Lewy bodies,in neurons.Recent studies have suggested that Lewy bodies are formed similarly to aggresomes,and results have supported the concept that the novel cellular organelle,the aggresome,is a cytoprotective response that sequesters and facilitates clearance of potentially toxic protein aggregates.In addition,α-tubulin deacetylase has been shown to regulate aggresome formation and rescue neural cell viability in response to misfolded protein.Therefore,the regulation of aggresome formation to trigger cellular self-protection system could arrest PD progression.The present study discusses research progress related to Lewy bodies,aggresomes,and histone deacetylases,with an emphasis on histone deacetylase 6 and sirtuin type 2. | Guoyi Li Ming Chang Huiyi Jiang Hongrong Xie Xinyu Hu Linsen Hu | 2011 | Neural Regeneration Research2011，6，2： | 0 |
| 10 | Valproic acid alters differential protein expression in SH-SY5Y cells显示文摘This study sought to identify differentially expressed proteins in SH-SY5Y cells treated with valproic acid, using two-dimensional difference gel electrophoresis analysis. Three proteins were unambi-guously identified: the eukaryotic translation initiation factor 4A isoform 1 and ATP6V1B2 protein were downregulated, while the heterogeneous nuclear ribonucleoprotein K was upregulated. Moreover, all three proteins are associated with altered expression due to oxidative stress. Ma-trix-assisted laser desorption/ionization-time of flight mass spectrometry and protein immunoblotting assay confirmed the differential expression of eukaryotic translation initiation factor 4A isoform 1. The results indicate that valproic acid exerts an antioxidation effect by regulating the expression of eukaryotic translation initiation factor 4A isoform 1. | Zhong Dong Ying Wang Ming Chang Guoyi Li Linsen Hu | 2011 | Neural Regeneration Research2011，6，27： | 0 |
| 11 | Synthetic protease inhibitor-induced inclusions in PC12 cells Potential proteomic characterization of six subunits in the 26S proteasome显示文摘Proteasome dysfunction during dopaminergic degeneration induces proteolytic stress, and is a contributing factor for the onset and formation of Lewy bodies. Results from our previous studies showed that synthetic proteasome inhibitor-induced inclusions in PC12 cells contained six subunits in the 26S proteasome. In the present study, mass spectrometry analysis of single protein spots resolved by two-dimensional gel electrophoresis and identified by bioinformatic analysis of peptide mass fingerprint (PMF) data were performed to comprehensively characterize the proteomic profile of the proteasome subunits. Results showed that six subunits in the 26S proteasome were characterized through accurate assignment by PMF data-specific protein identification in protein databases. Additionally, identification of one of the proteasome subunits was further confirmed using a subunit-specific antibody against non-adenosine triphosphatase subunit 11 of the 19S regulatory particle. Results suggest that the potential proteomic profile of six subunits in the 26S proteasome could be established from proteasome inhibitor-induced inclusions in PC12 cells. | Mingxiu Tian Xing'an Li Yingjiu Zhang Yihong Hu Ming Chang Tao Liu Danping Wang Yu Zhang Linsen Hu | 2010 | Neural Regeneration Research2010，5，22： | 0 |
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