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| 1 | Nonlinear Dynamic Analysis of Flexible Marine-Risers显示文摘A nonlinear dynamic analysis model is estabilished on the basis of 'lumped mass' approach, which takes the influence of the fluid flow within the pipe into consideration. Numerical results are compared with the published works, and the effects of internal fluid flow, internal pressure, dyanmics as well as the nonlinear characteristics on the behavior of flexible risers are discussed. From this work, some useful conclusions are drawn. | Chen Jiajing and Wang Dongjiao Professor, South China University of Technology, Guangzhou PhD Student, Hong Kong Polytechnic Formerly, South China University of Technology, Guangzhou | 1991 | China Ocean Engineering1991，6，4： | 2 |
| 2 | IL-33 drives the antitumor effects of dendritic cells via the induction of Tc9 cells显示文摘Dendritic cell(DC)tumor vaccines exert their antitumor effects through the induction of effector T cells.We recently identified Tc9 cells as a new potent antitumor effector T cell subset.However,approaches to direct DCs to preferably prime antitumor Tc9 cells should be further exploited.Here,we demonstrate that the addition of interleukin(IL)-33 potently promotes the induction of Tc9 cells by DCs in vitro and in vivo.IL-33 treatment also drives the cytotoxic activities of DC-induced Tc9 cells.Notably,IL-33 treatment enhances cell survival and proliferation of DC-primed CD8+T cells.More importantly,the addition of IL-33 during in vitro priming of tumor-specific Tc9 cells by DCs increases the antitumor capability of Tc9 cells.Mechanistic studies demonstrated that IL-33 treatment inhibits exhaustive CD8+T cell differentiation by inhibiting PD-1 and 2B4 expression and increasing IL-2 and CD127(IL-7 receptor-α,IL-7Rα)expression in CD8+T cells.Finally,the addition of IL-33 further promotes the therapeutic efficacy of DC-based tumor vaccines in the OT-I mouse model.Our study demonstrates the important role of IL-33 in DC-induced Tc9 cell differentiation and antitumor immunity and may have important clinical implications. | Ning Liu Yuxue Jiang Jintong Chen He Nan Yinghua Zhao Xiao Chu Alison Wang Dongjiao Wang Tianxue Qin Sujun Gao Ying Yue Siqing Wang | 2019 | Cellular & Molecular Immunology2019，16，7： | 2 |
| 3 | The numerical prediction of draghead motion of trailing suction Hopper dredger in time domain显示文摘 | Chen Zhanglan Ye Jiawei Wang Dongjiao | 2014 | Ocean Engineering2014，91，： | 1 |
| 4 | Non-enzymatic detection of hydrogen peroxide using a functionalized three-dimensional graphene electrode显示文摘 | Fengna Xi Dongjiao Zhao Xuewan Wang Peng Chen | 2013 | Electrochemistry Communications2013，，： | 1 |
| 5 | Clonal Growth of Hum an Acute Leukem ia Cells in Serum -free Methylcellulose Medium显示文摘The clonal growth of human acute leukemia cell line (K562) and acute myeloid leukemia cells in the serum free culture (SFC) was studied in order to establish a SFC system which could replace the effects of serum by using semi solid methylcellulose culture technique. Our results showed that the clonal growth of K562 cells in semi solid culture was dependent on exogenous serum. The K562 could be grown in SFC supplemented with 4 major replacing substances. The multifactor and multilevel orthogonal experiment demonstrated that the colony formation was statistically influenced by the 4 replacing substances at various concentrations ( P <0.01). Among them, bovine serum albumin had greatest effect on clonal growth of K562 cells with the optimal concentration being 15 mg/L, followed by transferring, cholesterol and insulin with their optimal concentrations being of 150 mg/L, 7.8 mg/L and 7.0 mg/L respectively. SFC system was formed with the 4 substances at their optimal concentrations. Colony formation of the blast cells in 10 patients with acute myeloid leukemia was observed in this SFC system. There was a heterogeneity of acute myeloid leukemia cells among the 10 patients in response to the growth substances. In SFC system, there was a linear relationship between the number of the clonal formation and the count of the added cells, indicating the colony growth of the cells. Primary acute leukemia cells maintained in SFC system in 10 cases could completely form clones. The colony formation number in some cases in SFC system was more than that of the serum containing culture. The SFC system could partially replace the serum for study of the clonal formation of human leukemia cells. | CHEN Zhichao , ZOU Ping , YOU Yong , LIU Zhongping , XIANG Jianping , YU Dongjiao Institute of Hematology, Tongji Medical University, Wuhan 430030 | 1999 | Journal of Huazhong University of Science and Technology(Medical Sciences)1999，19，3： | 0 |
| 6 | The Com prehensive Evaluation on Four Indices of Drug Re-sistance in Acute Myeloid Leukem ia显示文摘To study sensitivity of drug resistance indexes and resistance manner in acute myeloid leukemia (AML), MTT drug sensitivity, growth types of CFU L in vitro , Bcl 2 antigen and Bcl 2/Bax ratio and intracellular fluorescence intensity of daunorubicin (DNR) were determined. In 62 cases of AML, the positive coincidence rate was 73 % with MTT test and the negative coincidence rate was 70 %. In 3 commonly used drugs, if one drug showed sensitivity, the coincidence remission rate reached 71 %. In 51 cases of AML, there were 31 patients in the group of complete remission (CR), in which CFU L of 29 patients showed independent growth. CFU L of 2 patients showed no growth. However, there were 20 patients in the group of non remission (NR), in which CFU L of 14 patients showed independent growth. CFU L of 6 patients showed non growth pattern. Statistical analysis showed significant difference ( P <0.05). In 32 cases of AML, the expression rate of Bcl 2 was 59.55 %±19.56 % in drug sensitive group, and one was 77.36 %±11.91 % in drug resistant group, respectively ( P <0.05). At the same time, the ratio of Bcl 2/Bax was 7.50±5.04 in drug sensitive group and one was 14.32±8.99 in drug resistant group, respectively ( P <0.05). In 15 case of clinically drug resistant AML, the fluorescence histogram of DNR showed left shift of main peak (LSMP) in 12 patients. They were diagnosed as classical drug resistance. Meanwhile, 1 patient showed right shift of main peak (RSMP) in 3 patients. They were diagnosed as re growth drug resistance. It is concluded that MTT and CFU L might be used for prediction of drug sensitivity or resistance when patients were on treatment. Bcl 2 and ratio of Bcl 2/Bax might be associated with the prognosis. DNR histogram could be employed for identify the pattern drug resistance. The strength and weakness of these techniques were discussed. | CHEN Yan , HE Mingsheng , XIANG Zhifu , WU Yudan, YUE Beibei , YU Dongjiao, LI Huiyu Institute of Hematology, Xiehe Hospital, Tongji Medical University, Wuhan, 430022 | 1999 | Journal of Huazhong University of Science and Technology(Medical Sciences)1999，19，3： | 0 |
| 7 | Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway显示文摘Emerging evidence suggested that zinc finger protein 831(ZNF831)was associated with immune activity and stem cell regulation in breast cancer.Whereas,the roles and molec-ular mechanisms of ZNF831 in oncogenesis remain unclear.ZNF831 expression was significantly diminished in breast cancer which was associated with promoter CpG methylation but not mu-tation.Ectopic over-expression of ZNF831 suppressed breast cancer cell proliferation and col-ony formation and promoted apoptosis in vitro,while knockdown of ZNF831 resulted in an opposite phenotype.Anti-proliferation effect of ZNF831 was verified in vivo.Bioinformatic analysis of public databases and transcriptome sequencing both showed that ZNF831 could enhance apoptosis through transcriptional regulation of the JAK/STAT pathway.ChiP and luciferase report assays demonstrated that ZNF831 could directly bind to one specific region of STAT3 promoter and induce the transcriptional inhibition of STAT3.As a result,the attenuation of STAT3 led to a restraint of the transcription of Bcl2 and thus accelerated the apoptotic progression.Augmentation of STAT3 diminished the apoptosis-promoting effect of ZNF831 in breast cancer cell lines.Furthermore,ZNF831 could ameliorate the anti-proliferation effect of capecitabine and gemcitabine in breast cancer cell lines.Our findings demonstrate for the first time that ZNF831 is a novel transcriptional suppressor through inhibiting the expression of STAT3/Bcl2 and promoting the apoptosis process in breast cancer,suggesting ZNF831 as a novel biomarker and potential therapeutic target for breast cancer patients. | Jun Fan Zhe Zhang Hongqiang Chen Dongjiao Chen Wenbo Yuan Jingzhi Li Yong Zeng Shimeng Zhou Shu Zhang Gang Zhang Jiashen Xiong Lu Zhou Jing Xu Wenbin Liu Yan Xu | 2024 | Genes & Diseases2024，11，1： | 0 |
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