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| 1 | Design of a multiplex PCR method for detection of toxigenicpathogenic in Vibrio cholerae显示文摘Objective:To study virulence and regulatory genes(hlyA,ctxB,tcpI) in clinical strains of Vibrio ckolerae(V.cholerae),simultaneously.Methods:Three important genes,tepI,hlyA and ctxB were used for detection of toxigenic and pathogenic V.cholera by chain reaction assay method. Results:According to the results of the PCR,the incidence of hlyA,tcpI,and ctxB genes in clinical isolates was obtained as 94.7%(72 sample),90.8%(69 sample),and 92.1%(70 sample), respectively.Five strains possessed all genes except ctxB,six strains possessed all genes except tcpI,four strains possessed all genes except hlyA,one strain possessed only hlyA and 60 strains contained a combination of three genes.Including hlyA,ctxB and tcpI,Conclusions:Result show that this method could be reliable to detect toxigenic-pathogenic strains of V.cholerae in Iran. | Abbas Ali Imani Fooladi Iman Islamieh D Hosseini Doust R Karami A Marashi SM | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，2： | 2 |
| 2 | The role of BNP testing in heart failure显示文摘 | Doust J Lehman R Glasziou P | 2006 | Am Fam Physician2006，7，11： | 1 |
| 3 | The role of BNP testing in heart failure显示文摘 | Doust J Lehman R Glasziou P | 2006 | Am Faro Physician2006，74，11： | 1 |
| 4 | Petroleum systems of Indonesia 显示文摘 | Doust H Noble R A | 2008 | Marine and Petroleum Geology2008，41，25： | 1 |
| 5 | The role of BNP testing in heart failure显示文摘 | Doust J Lehman R Glasziou P | 2006 | Am Fam Physician2006，74，11： | 1 |
| 6 | Early invasiveversus conservative strategies for unstable angina&non ST-elevation myocardial infarction in the stent era显示文摘 | Hoenig M R Doust J A Aroney C N | 2006 | CochraneDatabase Syst Rev2006，17，00： | 1 |
| 7 | Preventing over-diagnosis: How to stop harming the healthy显示文摘 | Moynihan R Doust J Henry D | 2012 | BMJ2012，344，： | 1 |
| 8 | Preventing over diagnosis:how to stop harming the healthy显示文摘 | Moynihan R Doust J Henry D | 2012 | BMJ2012，344，： | 1 |
| 9 | Prevalence of attention- deficit/hyperactivity disorder: a systematic review and meta- analysis显示文摘 | Thomas R Sanders S Doust J | 2015 | Pediatrics2015，135，4： | 1 |
| 10 | Petroleum systems of Indonesia显示文摘 | Doust H Noble R A | 2008 | Marine and Petroleum Geology2008，25，2： | 1 |
| 11 | Wooden orbital foreign body in a Weimaraner显示文摘 | Hartley C McConnell J F Doust R | 2007 | Vet Ophthalmol2007，10，6： | 1 |
| 12 | Petroleum systems of Indonesia显示文摘 | Doust H Noble A R | 2008 | Marine and Petroleum Geology2008，25，2： | 1 |
| 13 | The role of BNP testing inheartaiIure 显示文摘 | Doust J Lehman R Glasziou P | 2006 | AmFamPhysician2006，74，1： | 1 |
| 14 | Reference genome sequence of the model plant Setaria显示文摘 | Bennetzen J L Schmutz J Wang H Percifield R Hawkins J Pontaroli A C Estep M Feng L Vaughn J N Grimwood J Jenkins J Barry K Lindquist E Hellsten U Deshpande S Wang X Wu X Mitros T Triplett J Yang X Ye C Y Mauro-Herrera M Wang L Li P Sharma M Sharma R Ronald P C Panaud O Kellogg E A Brutnell T P Doust A N Tuskan G A Rokhsar D and Devos K M | 2012 | Nature Biotechnology2012，30，6： | 1 |
| 15 | The role of BNP testing in heart failure显示文摘 | Doust J Lehman R Glasziou P | 2006 | Am Faro Physician2006，74，11： | 1 |
| 16 | The role of BNP testing in heart failure显示文摘 | Doust J Lehman R Glasziou P | 2006 | Am Faro Physician2006，，： | 1 |
| 17 | Petroleum systems of Indonesia显示文摘 | DOUST H NOBLE R A | 2008 | Marine and Petroleum Geology2008，，25： | 1 |
| 18 | Preventing overdiagnosis: how to stop harming the healthy显示文摘 | Moynihan R Doust J Henry D | 2012 | BMJ2012，344，1： | 1 |
| 19 | Preventing over diagnosis: how to stop harming the healthy 显示文摘 | Moynihan R Doust J Henry D | 2012 | BMJ2012，344，： | 1 |
| 20 | The role of BNP teating in heart failure显示文摘 | Doust J Lehman R Glasziou P | 2006 | Am Fam Physician2006，7，11： | 1 |
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