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| 1 | Advances in cellulose ester performance and application显示文摘 | Kevin J Edgar Charles M Buchanan John S Debenham Paul A Rundquist Brian D Seiler Michael C Shelton Debra Tindall | 2001 | Progress in Polymer Science2001，，9： | 2 |
| 2 | Progress in structural materials for aerospace systems 显示文摘 | James C W Edgar A S | 2003 | Acta Materialia2003，51，： | 1 |
| 3 | Run-to-run control and performance monitoring of overlay in semiconductor manufacturing 显示文摘 | BODE C A KO B S EDGAR T F | 2004 | Control Engineering Practice2004，12，7： | 1 |
| 4 | Effects of Eimeria mitis on egg pro- duction of Single-Comb White Leghorn hens 显示文摘 | Fitz-Coy S H and Edgar S A | 1992 | Avain Diseases1992，36，： | 1 |
| 5 | Reduced intestinal absorption in broilers during Eimeria mitis infection 显示文摘 | Ruff M D and Edgar S A | 1982 | Am J Vet Res1982，43，3： | 1 |
| 6 | Design robust nonlinear controllers for autonomous underwater vehicle with comparison simulated and at-sea test data显示文摘 | SONG Fei-jun Edgar A N SMITH S M | 2002 | Journal of vibration and control2002，，8： | 1 |
| 7 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 8 | Photoluminescence and crystallization in europium-doped fluorobromozirconate glass-ceramics显示文摘 | Edgar A Schweizer S Assmann S | 2001 | Journal of Non-Crystalline Solids2001，284，3： | 1 |
| 9 | Semireusable launch vehicle: a next-generation launch vehicle 显示文摘 | Edgar A S | 2001 | J of Spacecraft and Rockets2001，38，3： | 1 |
| 10 | Progress in structural materials for aerospace systems 显示文摘 | JAMES C W EDGAR A S | 2003 | Acta Mater2003，51，： | 1 |
| 11 | The effect of ambient lighton QLF analyses 显示文摘 | PRETTY I A EDGAR W M HIGHAM S M | 2002 | J Oral Rehabil2002，29，4： | 1 |
| 12 | Stable coccidiosis immunization显示文摘 | Edgar S A | 1964 | US Patent1964，3，5： | 1 |
| 13 | What does it cost to produce a click 显示文摘 | Edgar S A | 1973 | Poultry Digest1973，32，： | 1 |
| 14 | Vibrational analysis of the dioctahedral mica: 2M1 muscovite 显示文摘 | Mckeown D A Bell M I and Edgar S E | | American Mineralogist0，84，： | 1 |
| 15 | Taurine status in cats is not maintained by dietary cysteinesulfinic acid显示文摘 | Edgar S E Claudia A K Quinton R R | 1998 | J Nutr1998，128，： | 1 |
| 16 | Lung function and airway inflammation in rats following exposure to combustion products of carbon-graphite/epoxy composite material: comparison to a rodent model of acute lung injury显示文摘 | Gregory S Whitehead Keith A Grasman Edgar C Kimmel | 2003 | Toxicology2003，183，13： | 1 |
| 17 | Dietary cysteic acid serves as a precursor of taurine for cats显示文摘 | Edgar S E Mary A H Melanie M M | 1994 | J Nutr1994，124，： | 1 |
| 18 | Core outcomes for adult burn survivors: a clinical overview 显示文摘 | Falder S Browne A Edgar D | 2009 | Burns2009，35，5： | 1 |
| 19 | Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats显示文摘 | Rocío V. Pérez-Gallardo Ruth Noriega-Cisneros Edgar Esquivel-Gutiérrez Elizabeth Calderón-Cortés Christian Cortés-Rojo Salvador Manzo-Avalos Jesús Campos-García Rafael Salgado-Garciglia Rocío Montoya-Pérez Istvan Boldogh Alfredo Saavedra-Molina | 2014 | Journal of Bioenergetics and Biomembranes2014，，6： | 1 |
| 20 | Progress in structural materials for aerospace systems 显示文摘 | WILLIAMS J C EDGAR A S J | 2003 | Acta Materialia2003，59，： | 1 |
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