| 1 | In-situ hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA damage显示文摘Mitochondrial DNA has a special structure that is prone to damage resulting in many serious diseases,such as genetic diseases and cancers.Therefore,the rapid and specific monitoring of mitochondrial DNA damage is urgently needed for biological recognition.Herein,we constructed an in situ hydrophobic environment-triggering reactive fluorescence probe named MBI-CN.The fluorophore was 2-styrene-1H-benzo[d]imidazole,and malononitrile was introduced as a core into a molecule to initiate the hydrolysis reaction in the specific environment containing damaged mitochondrial DNA.In this design,MBI-CN conjugates to mitochondrial DNA without causing additional damages.Thus,MBI-CN can be hydrolyzed to generate MBI-CHO in an in situ hydrophobic environment with mitochondrial DNA damage.Meanwhile,MBI-CHO immediately emitted a significative fluorescence signal changes at 437 and 553 nm within 25 s for the damaged mitochondria DNA.Give that the specific and rapid response of MBI-CN does not cause additional damages to mitochondrial DNA,it is a potentially effective detection tool for the real-time monitoring of mitochondrial DNA damage during cell apoptosis and initial assessment of cell apoptosis. | Beidou Feng Huiyu Niu Hongchen Zhai Congcong Shen Hua Zhang | 2022 | Frontiers of Chemical Science and Engineering2022，16，1： | 2 |
| 3 | Polypyrrole Hollow Nanotubes Loaded with Au and Fe_(3)O_(4) Nanoparticles for Simultaneous Determination of Ascorbic Acid,Dopamine,and Uric Acid显示文摘An ultrasensitive electrochemical biosensor was fabricated for electroanalytical determination of ascorbic acid(AA),dopamine(DA)and uric acid(UA)individually and simultaneously based on polypyrrole hollow nanotubes loaded with Au and Fe_(3)O_(4) nanoparticles(NPs)uniformly(PPy@Au-Fe_(3)O_(4)).The PPy@Au-Fe_(3)O_(4) nanotubes were synthesized in one-pot using MoO_(3) nanorods as templates and the polymerization of Py,the formation of Au and Fe_(3)O_(4) NPs and the removel of MoO_(3) templates took place stimultaneously.Electrochemical studies reveal that PPy@Au-Fe_(3)O_(4) modified glassy carbon electrode(GCE)possesses excellent electrocatalytic activities toward the oxidation of AA,DA and UA.Their oxidation peak currents increase linearly in the concentration ranges of 1–2000µmol/L for AA,0.01–25 and 25–300µmol/L for DA and 0.1–300µmol/L for UA.Their detection limit values(S/N=3)were calculated as 0.45,0.0049,and 0.051µmol/L for AA,DA and UA in the individual detection.By changing the concentrations simultaneously,the calibration curves showed linearity to 1000,200,and 200µmol/L with detection limit of 0.39,0.0060,and 0.060µmol/L for AA,DA,and UA,respectively.Finally,the obtained biosensor was successfully applied to the detection of AA,DA,and UA with satisfactory results on actual samples. | SHEN Congcong CHEN Yuehua FENG Beidou CHI Hongying ZHANG Hua | 2022 | Chemical Research in Chinese Universities2022，38，4： | 0 |
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