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| 1 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 2 | Promotion of oxygen transfer in three-phase fluidized-bed bioreactors by floating bubble breakers显示文摘 | Kang Y Fan L T Min B T | 1991 | Biotechnol Bioeng1991，37，： | 1 |
| 3 | A Novel Strategy to Fabricate γ-Fe2O3-MWCNTs Hybrids with Selectively Ferromagnetic or Superparamagnetic Properties 显示文摘 | Fan X B Tan F Y Zhang G L | 2007 | Mater Sci & Eng A2007，45，： | 1 |
| 4 | Treatment and reuse of toilet wastewaterby an airlift external circulation membrane bioreactor显示文摘 | Fan Y B Li G Wu L L | 2006 | Process Bio-chemistry(Amsterdam Netherlands)2006，41，6： | 1 |
| 5 | Clinical features of Hirayama disease in mainland China 显示文摘 | Zhou B Chen L Fan D | 2010 | Amyotroph Lateral Scler2010，11，12： | 1 |
| 6 | Constacyclic codes over finite fields显示文摘 | Chen B Fan Y Lin L | 2012 | Finite Fields and Their Applications2012，18，： | 1 |
| 7 | The TIL commissioning and performance显示文摘 | X Zhang W Zheng X Wei F Jing Z Sui K Zheng Q Xu X Yuan X Jiang L Yang P Ma M Li J Wang D Hu S He F Li Z Peng B Feng H Zhou L Guo X Li X Zhang J Su Q Zhu H Yu R Zhao C Ma H He D Fan W Zhang | 2008 | Journal of Physics: Conference Series2008，，3： | 1 |
| 8 | Free vibrations of tapered piles embedded partially in Winkler type foundations显示文摘 | LEE B K JEONG J S FAN L G JIN T K | 1999 | J Civil Engrg1999，3，2： | 1 |
| 9 | Process configurations adapted to membrane bioreactors for enhanced biological phosphorus and nit rogen removal 显示文摘 | L ESJ FAN B GNIRSS R ADAM C | 2002 | Desalination2002，149，： | 1 |
| 10 | Fertility sparing therapy for ovarian cancer has inherent risks and benefits显示文摘 | Douglas N C Fan L Pothuri B | 2005 | Arch Gynecol Obstet2005，272，4： | 1 |
| 11 | Isolation and Structur- al Study on Carbohydrates from Cynanchum otophyllum and Cynanchum paniculatum 显示文摘 | Zhao Y B Fan Q S Xu G L | 2008 | Journal of Carbohy- drate Chemistry2008，27，7： | 1 |
| 12 | Natural variation in GS5 plays an important role in regulating grain size and yield in rice显示文摘 | Li Y B Fan C C Xing Y Z Jiang Y H Luo L J Sun L Shao D Xu C J Li X H Xiao J H He Y Q Zhang Q F | 2011 | Nature genetics2011，2，43： | 1 |
| 13 | Survival and reproduction of Liposcelis paeta ( Pearman ) (Psocoptera: Liposcelididae) when irradiated by electron beams 显示文摘 | Wang J J Guo D Q Fan J L Chen Y T Zhang J W Yang B A Li X Z | 2012 | Entomological Science2012，15，1： | 1 |
| 14 | CD40 agonist antibody mediated improvement of chronic Cryptosporidium infection in patients with X-linked hyper IgM syndrome显示文摘 | Fan X Upadhyaya B Wu L | 2012 | Clin Immunol2012，143，2： | 1 |
| 15 | Surface hydrophobic modification of starch with bio-based epoxy resins to fab- ricate high-performance polylaetide composite materials 显示文摘 | Xiong Z Ma S Q Fan L B | 2014 | Compos Sci Techno12014，94，9： | 1 |
| 16 | Improving crop productivity and resource use efficiency to ensure food security and environmental quality in China显示文摘 | Fan M S Shen J B Yuan L X | 2012 | Journal of Experimental Botany2012，63，1： | 1 |
| 17 | Immune response and inhibi- tion of bacterial growth by electrotransfer of plasmid DNA containing the antimicrobial peptide,epinecidin - 1,into zebrafish muscle 显示文摘 | LIN S B FAN T W WU J L | 2009 | Fish Shellfish Immunol2009，26，3： | 1 |
| 18 | Hot Deformation and Processing Map of 15% SiCp/2009 Al Composite 显示文摘 | Xiao B L Fan J Z Tian X F | 2005 | Journal of Materials Science2005，40，： | 1 |
| 19 | Deposition of Gd2Zr207 single buffer layers with different thickness for YBa2Cu3O7-δ coated conductors on metallic substrates 显示文摘 | Y1NG L L FAN F GAO B | 2010 | Phys C2010，470，1314： | 1 |
| 20 | An evaluation of the Truview EVO2 laryngoscope显示文摘 | Li J B Xiong Y C Wang X L Fan X H Li Y Xu H | 2007 | Anaesthesia2007，62，： | 1 |
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