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| 1 | Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury显示文摘We report the generation of human ESC-derived,expandable hepatic organoids(hEHOs)using our newly established method with wholly defined(serum-free,feeder free)media.The hEHOs stably maintain phenotypic features of bipotential liver stem/progenitor cells that can differentiate into functional hepatocytes or cholangiocytes.The hEHOs can expand for 20 passages enabling large scale expansion to cell numbers requisite for industry or clinical programs.The cells from hEHOs display remarkable repopulation capacity in injured livers of FRG mice following transplantation,and they differentiate in vivo into mature hepatocytes.If implanted into the epididymal fat pads of immune-deficient mice,they do not generate non-hepatic lineages and have no tendency to form teratomas.We further develop a derivative model by incorporating human fetal liver mesenchymal cells(hFLMCs)into the hEHOs,referred to as hFLMC/hEHO,which can model alcoholic liver disease-associated pathophysiologic changes,including oxidative stress generation,steatosis,inflammatory mediators release and fibrosis,under ethanol treatment.Our work demonstrates that the hEHOs have considerable potential to be a novel,ex vivo pathophysiological model for studying alcoholic liver disease as well as a promising cellular source for treating human liver diseases. | Shuyong Wang Xuan Wang Zuolong Tan Yuxin Su Juan Liu Mingyang Chang Fang Yan Jie Chen Tao Chen Chuanjiang Li Jie Hu Yunfang Wang | 2019 | Cell Research2019，29，12： | 20 |
| 2 | Connective tissue diseases in primary biliary cirrhosis:A population-based cohort study显示文摘AIM:To establish the frequency and clinical features of connective tissue diseases(CTDs)in a cohort of Chinese patients with primary biliary cirrhosis(PBC).METHODS:Three-hundred and twenty-two Chinese PBC patients were screened for the presence of CTD,and the systemic involvement was assessed.The differences in clinical features and laboratory findings between PBC patients with and without CTD were documented.The diversity of incidence of CTDs in PBC of different countries and areas was discussed.For the comparison of normally distributed data,Student’s t test was used,while non-parametric test(Wilcoxon test)for the non-normally distributed data and 2×2χ2or Fisher’s exact tests for the ratio.RESULTS:One-hundred and fifty(46.6%)PBC patients had one or more CTDs.The most common CTD was Sj gren’s syndrome(SS,121 cases,36.2%).There were nine cases of systemic sclerosis(SSc,2.8%),12of systemic lupus erythematosus(SLE,3.7%),nine of rheumatoid arthritis(RA,2.8%),and 10 of polymyositis(PM,3.1%)in this cohort.Compared to patients with PBC only,the PBC+SS patients were more likely to have fever and elevated erythrocyte sedimentation rate(ESR),higher serum immunoglobulin G(IgG)levels and more frequent rheumatoid factor(RF)and interstitial lung disease(ILD)incidences;PBC+SSc patients had higher frequency of ILD;PBC+SLE patients had lower white blood cell(WBC)count,hemoglobin(Hb),platelet count,γ-glutamyl transpeptidase and immunoglobulin M levels,but higher frequency of renal involvement;PBC+RA patients had lower Hb,higher serum IgG,alkaline phosphatase,faster ESR and a higher ratio of RF positivity;PBC+PM patients had higher WBC count and a tendency towards myocardial involvement.CONCLUSION:Besides the common liver manifestation of PBC,systemic involvement and overlaps with other CTDs are not infrequent in Chinese patients.When overlapping with other CTDs,PBC patients manifested some special clinical and laboratory features which may have effect on the prognosis. | Li Wang Feng-Chun Zhang Hua Chen Xuan Zhang Dong Xu Yong-Zhe Li Qian Wang Li-Xia Gao Yun-Jiao Yang Fang Kong Ke Wang | 2013 | World Journal of Gastroenterology2013，19，31： | 18 |
| 3 | Blood-letting punctures at twelve Jing-Well points of the hand can treat cerebral ischemia in a similar manner to mannitol显示文摘A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method.Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the hand,and/or by injecting mannitol into the caudal vein twice daily.Brain tissue was collected at 24,48 and 72 hours after modeling,and blood was collected through the retinal vein before Evans blue was injected,approximately 1 hour prior to harvesting of brain tissue.Results showed that Evans blue leakage into brain tissue and serum nitric oxide synthase activity were significantly increased in model rats.Treatment with blood-letting punctures at twelve Jing-Well points of the hand and/or injection of mannitol into the caudal vein reduced the amount of Evans blue leakage into the brain tissue and serum nitric oxide synthase activity to varying degrees.There was no significant difference between single treatment and combined treatment.Experimental findings indicate that blood-letting punctures at twelve Jing-Well points of the hand can decrease blood-brain barrier permeability and serum nitric oxide synthase activity in rats following middle cerebral artery occlusion,and its effect is similar to that of mannitol injection alone and Jing-Well points plus mannitol injection. | Xuan Lu Zelin Chen Yi Guo Liang Gao Liyuan Jiang Zhongzheng Li Jianqiao Fang | 2013 | Neural Regeneration Research2013，8，6： | 14 |
| 4 | Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells显示文摘Mutations in the liver/bone/kidney alkaline phosphatase(Alpl) gene cause hypophosphatasia(HPP) and early-onset bone dysplasia,suggesting that this gene is a key factor in human bone development. However, how and where Alpl acts in bone ageing is largely unknown. Here, we determined that ablation of Alpl induces prototypical premature bone ageing characteristics, including bone mass loss and marrow fat gain coupled with elevated expression of p16^(INK4A)(p16) and p53 due to senescence and impaired differentiation in mesenchymal stem cells(MSCs). Mechanistically, Alpl deficiency in MSCs enhances ATP release and reduces ATP hydrolysis. Then, the excessive extracellular ATP is, in turn, internalized by MSCs and causes an elevation in the intracellular ATP level, which consequently inactivates the AMPKα pathway and contributes to the cell fate switch of MSCs. Reactivating AMPKα by metformin treatment successfully prevents premature bone ageing in Alpl+/-mice by improving the function of endogenous MSCs.These results identify a previously unknown role of Alpl in the regulation of ATP-mediated AMPKα alterations that maintain MSC stemness and prevent bone ageing and show that metformin offers a potential therapeutic option. | Wenjia Liu Liqiang Zhang Kun Xuan Chenghu Hu Shiyu Liu Li Liao Bei Li Fang Jin Songtao Shi Yan Jin | 2018 | Bone Research2018，6，4： | 9 |
| 5 | Factors affecting occurrence of gastric varioliform lesions: A case-control study显示文摘AIM: To investigate the factors influencing the occurrence of gastric varioliform lesions(GVLs) and their possible link with gastric cancer.METHODS: A 1:1 matched case-control study was performed to retrospectively analyze data from 1638 chronic gastritis patients who had undergone gastroscopy at one of two Chinese hospitals between 2009 and 2014. Patients with GVLs(cases) were compared to those without such lesions(controls). Endoscopic and pathological findings were recorded, along with interview information on Helicobacter pylori(H. pylori) infection, medical, drug and family histories, lifestyle and eating habits. The association between each factor and the occurrence of GVLs was estimated, and then multivariate conditional logistic regression was used to evaluate the independent factors.RESULTS: The frequency and severity of glandular atrophy, intestinal metaplasia(IM) and low-grade intraepithelial neoplasia were significantly increased in the GVL group(P < 0.01). Overall analysis showed that H. pylori infection [3.051(2.157, 4.317), P <0.001], allergic respiratory diseases [3.636(2.183, 6.055), P < 0.001], work-related stress [2.019(1.568, 2.600), P < 0.001], irregular meals [2.300(1.462, 3.619), P < 0.001], high intake of spicy food [1.754(1.227, 2.507), P = 0.002] and high intake of fresh fruit [0.231(0.101, 0.529), P = 0.001] were significantly correlated with the occurrence of GVLs(positively, except for the latter). Stratified analyses indicated that pickled food consumption in patients over 50 years old [7.224(2.360, 22.115), P = 0.001] and excessive smoking in men [2.013(1.282, 3.163), P = 0.002] were also positively correlated, and that, for antral GVLs, vegetable consumption [0.491(0.311, 0.776), P = 0.002] was negatively correlated. CONCLUSION: Seven risk factors and two protective factors are determined for GVLs, which were found to be associated with premalignant abnormalities. | Tian-Hui Zou Ru-Hua Zheng Qin-Yan Gao Xuan Kong Xiao-Yu Chen Zhi-Zheng Ge Ying-Xuan Chen Xiao-Ping Zou Jing-Yuan Fang | 2016 | World Journal of Gastroenterology2016，22，22： | 9 |
| 6 | Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice显示文摘Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant immunity.However,how NLRs are regulated and activate defense signaling is not fully understood.The rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t accumulation.In this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome pathway.OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta.The osvoz1 and osvoz2 single mutants display modest but opposite M.oryzae resistance in the non-Piz-t background.However,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to M.oryzae in the non-Piz-t background.Both OsVOZ1 and OsVOZ2 interact with Piz-t.Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to M.oryzae containing AvrPiz-t.Taken together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity. | Jiyang Wang Ruyi Wang Hong Fang Chongyang Zhang Fan Zhang Zeyun Hao Xiaoman You Xuetao Shi Chan Ho Park Kangyu Hua Feng He Maria Bellizzi Kieu Thi Xuan Vo Jong-Seong Jeon Yuese Ning Guo-Liang Wang | 2021 | Molecular Plant2021，14，2： | 8 |
| 7 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 8 | The impact of both platinum-based chemotherapy and EGFR-TKIs on overall survival of patients with advanced non-small cell lung cancer显示文摘Both platinum-based doublet chemotherapy(PBC) and epidermal growth factor receptor tyrosine kinase inhibitors(EGFR-TKIs) prolong the survival of patients with advanced non-small cell lung cancer(NSCLC). In early studies, most patients underwent PBC as first-line treatment, but not all patients could afford EGFR-TKIs as second-line treatment. To understand the impact of PBC and EGFR-TKIs on NSCLC prognosis, we evaluated the association between the receipt of both regimens and overall survival(OS). Using MEDLINE and EMBASE, we identified prospective, randomized, controlled phase III clinical trials in advanced NSCLC that met the inclusion criteria: in general population with advanced NSCLC, the percentage of patients treated with both PBC and EGFR-TKIs was available in the trial and OS was reported. After collecting data from the selected trials, we correlated the percentage of patients treated with both PBC and EGFR-TKIs with the reported OS, using a weighted analysis. Fifteen phase III clinical trials—involving 11,456 adult patients in 32 arms—were included in the analysis, including 6 trials in Asian populations and 9 in non-Asian(predominantly Caucasian) populations. The OS was positively correlated with the percentage of patients treated with both PBC and EGFR-TKIs(r = 0.797, P < 0.001). The correlation was obvious in the trials in Asian populations(r = 0.936, P < 0.001) but was not statistically significant in the trials in predominantly Caucasian populations(r = 0.116, P = 0.588). These results suggest that treatment with PBC and EGFR-TKIs may provide a survival benefit to patients with advanced NSCLC, highlighting the importance of having both modalities available for therapy. | Jian-Wei Zhang Yuan-Yuan Zhao Ying Guo Cong Xue Zhi-Huang Hu Yan Huang Hong-Yun Zhao Jing Zhang Xuan Wu Wen-Feng Fang Yu-Xiang Ma Li Zhang | 2014 | Chinese Journal of Cancer2014，33，2： | 7 |
| 9 | Exogenous Hematin Alleviates Mercury-induced Oxidative Damage in the Roots of Medicago sativa显示文摘在现在的学习,我们评估了保护的效果外长他我 oxygenase-1 (HO-1 EC 1.14.99.3 ) 对在苜蓿(Medicago sativa L.) 的根的导致水银的氧化损坏的 inducer 血色素。暴露于水银(HgCl ) 的植物每根延伸的氧化,以及抑制展出了类脂化合物的重要增加。然而,到对待的 HgCl (100 omol/L ) 的补充有效地种的血色素(50 oM ) 每氧化减少了类脂化合物并且部分增加了根延伸。这些回答被一氧化碳(公司) 的水溶液的申请与 50% 浸透模仿。另外,有血色素的处理能在根纸巾导致 HO-1 抄本的有势力正式就职,作为检测 12 h 列在后面处理。而且,抗氧化剂酶,包括的谷胱甘肽 reductase , monodehydroascorbate reductase 和超级氧化物 dismutase 活动的激活,和 lipoxygenase 活动的减少,被血色素在 12 h 或 24 h 导致,它被每膜正直的氧化和损失的为类脂化合物的察觉的组织化学的染色进一步证实。而, ascorbate 过氧化物酶和 guaiacol 过氧化物酶 isozyme 活动或他们的抄本被减少分别地,显示那过氧化氢可能充当一个信号调停在处理的开始的 Hg 忍耐。血色素的改善效果是特定的,自从 CO scavenger 血红素差别颠倒了上述行动。总起来说,我们的结果建议血色素在保护植物免于导致 Hg 的氧化损坏展出一个重要角色。 | Yi Han Wei Xuan Tian Yu Wen-Bing Fang Tian-Ling Lou Yin Gao Xiao-Yue Chen XiaoXiao Wen-Biao Shen | 2007 | Journal of Integrative Plant Biology2007，49，12： | 7 |
| 10 | Os isotope dating and growth hiatuses of Co-rich crust from central Pacific显示文摘Up to now, accurate determination of the growth age and hiatuses of the Co-rich crust is still a difficult work, which constrains the researches on the genesis, growth process, controlling factors, regional tectonics, paleo-oceanographic background, etc. of the Co-rich crust. This paper describes our work in determining the initial growth age of the Co-rich crust to be of the late Cretaceous Campanian Stage (about 75-80 Ma), by selecting the Co-rich crust with clear multi-layer structures in a central Pacific seamount for layer-by-layer sample analysis and using a number of chronological methods, such as Co flux dating, dating by correlation with 187Os/188Os evolution curves of seawater, and stratigraphic divi- sion by calcareous nannofossils. We have also discovered growth hiatuses with different time intervals in the early Paleocene, middle Eocene, late Eocene and early-middle Miocene, respectively. These re- sults have provided an important age background for further researches on the Co-rich crust growth process and the paleo-oceanographic environment evolution thereby revealed in the said region. | LI JiangShan FANG NianQiao QU WenJun DING Xuan GAO LianFeng WU ChangHang ZHANG ZhenGuo | 2008 | Science China Earth Sciences2008，51，10： | 6 |
| 11 | Etomidate affects the anti-oxidant pathway to protect retinal ganglion cells after optic nerve transection显示文摘Our previous studies revealed that etomidate, a non-barbiturate intravenous anesthetic agent, has protective effects on retinal ganglion cells within 7 days after optic nerve transection. Whether this process is related to anti-oxidative stress is not clear. To reveal its mechanism, we established the optic nerve transection injury model by transecting 1 mm behind the left eyeball of adult male Sprague-Dawley rats. The rats received an intraperitoneal injection of etomidate(4 mg/kg) once per day for 7 days. The results showed that etomidate significantly enhanced the number of retinal ganglion cells retrogradely labeled with Fluorogold at 7 days after optic nerve transection. Etomidate also significantly reduced the levels of nitric oxide and malonaldehyde in the retina and increased the level of glutathione at 12 hours after optic nerve transection. Thus, etomidate can protect retinal ganglion cells after optic nerve transection in adult rats by activating an anti-oxidative stress response. The study was approved by the Animal Ethics Committee at Air Force Medical University, China(approval No. 20180305) on March 5, 2018. | Xuan Zhao Fang Kuang Yi-Yan You Ming-Mei Wu Si-Wei You | 2019 | Neural Regeneration Research2019，14，11： | 6 |
| 12 | Glutamate Impairs Mitochondria Aerobic Respiration Capacity and Enhances Glycolysis in Cultured Rat Astrocytes显示文摘Objective To study the effect of glutamate on metabolism, shifts in glycolysis and lactate release in rat astrocytes. Methods After 10 days, secondary cultured astrocytes were treated with 1 mmol/L glutamate for 1 h, and the oxygen consumption rates(OCR) and extra cellular acidification rate(ECAR) was analyzed using a Seahorse XF 24 Extracellular Flux Analyzer. Cell viability was then evaluated by MTT assay. Moreover, changes in extracellular lactate concentration induced by glutamate were tested with a lactate detection kit. Results Compared with the control group, treatment with 1 mmol/L glutamate decreased the astrocytes' maximal respiration and spare respiratory capacity but increased their glycolytic capacity and glycolytic reserve. Further analysis found that 1-h treatment with different concentrations of glutamate(0.1-1 mmol/L) increased lactate release from astrocytes, however the cell viability was not affected by the glutamate treatment. Conclusion The current study provided direct evidence that exogenous glutamate treatment impaired the mitochondrial respiration capacity of astrocytes and enhanced aerobic glycolysis, which could be involved in glutamate injury or protection mechanisms in response to neurological disorders. | YAN Xu SHI Zhong Fang XU Li Xin LI Jia Xin WU Min WANG Xiao Xuan JIA Mei DONG Li Ping YANG Shao Hua YUAN Fang | 2017 | Biomedical and Environmental Sciences2017，30，1： | 6 |
| 13 | Exercise ameliorates the detrimental effect of chloroquine on skeletal muscles in mice via restoring autophagy flux显示文摘 | Dan JIANG Kai CHEN Xuan LU Hong-jian GAO Zheng-hong QIN Fang LIN | 2014 | Acta Pharmacologica Sinica2014，35，1： | 6 |
| 14 | Ordovician integrative stratigraphy and timescale of China显示文摘In this chapter, starting with a brief review of the research history and current status in the studies of the Ordovician chronostratigraphy in China, the subdivision of the Ordovician System, definition and recognition of its series and stage boundaries, and possible stratigraphic gaps are discussed in details in order to establish a multidisciplinary stratigraphic correlation through an integrated approach including lithostratigraphy, biostratigraphy, radiometric dating, chemostratigraphy and magnetostratigraphy. Being internationally accepted, the Ordovician System is now subdivided into three series and seven stages, in ascending order, Lower(Tremadocian, Floian), Middle(Dapingian, Darriwilian) and Upper series(Sandbian, Katian,Hirnantian). Three of the seven 'Golden Spikes' defining the bases of the Ordovician stages, which were established in 1997–2007, are located in China. As a regionally applied chronostratigraphy, the Ordovician System was subdivided in China into Lower(Xinchangian, Yiyangian), Middle(Dapingian, Darriwilian) and Upper series(Neichiashanian, Chientangkiangian,Hirnantian). This scheme agrees largely with the standard international classification, which can actually be directly applied to China, except for some special circumstances where the Neichiashanian and Chientangkiangian stages of the Upper Ordovician are used. Based on the new studies in recent years and distinctions and differences recognized in the development of the Ordovician System in the constituent terranes of China, a new framework for correlation among the major Chinese palaeoplates or terranes, e.g. South China, North China(including Tarim and Qaidam) and Xizang(Tibet)-western Yunnan, has been established. However, it has been recognized herein that uncertainties still remain on defining the base of the Tremadocian,Dapingian and Katian, and on the correlation between different mega-facies. More specifically, for the Tremadocian, the precise correlation of its base will depend on the better-defined conodont taxonomy, while for the Dapingian and Katian, on the correlation between different mega-facies. It is worthwhile to note that the chemostratigraphic studies of the Ordovician System in China produced the carbonate δ13 C curves for the Darriwilian(Middle Ordovician) and Katian(Upper Ordovician), which show significant differences from the composite global curve. Record of the Ordovician isotopic dating is relatively rare in China, with only three reliable ages from zircons that are all from the upper Katian to Hirnantian of the Upper Ordovician.Abundant bentonite beds in the Upper Ordovician of South China will also provide unique opportunities to advance the isotopic dating and related researches. Studies on the Ordovician magnetostratigraphy need to be significantly enhanced in China, as currently all the available results are restricted to the Lower Ordovician of North China, although they can be correlated with those known from other parts of the world. The analysis of the durational unevenness of the seven stages in the Ordovician supports the possibility to further subdivide the long-durational Tremadocian, Darriwilian and Katian stages, each into two substages. | Yuandong ZHANG Renbin ZHAN Yongyi ZHEN Zhihao WANG Wenwei YUAN Xiang FANG Xuan MA Junpeng ZHANG | 2019 | Science China Earth Sciences2019，62，1： | 6 |
| 15 | Pharmacokinetics-based identification of pseudoaldosterogenic compounds originating from Glycyrrhiza uralensis roots(Gancao)after dosing LianhuaQingwen capsule显示文摘LianhuaQingwen capsule,prepared from an herbal combination,is officially recommended as treatment for COVID-19 in China.Of the serial pharmacokinetic investigations we designed to facilitate identifying LianhuaQingwen compounds that are likely to be therapeutically important,the current investigation focused on the component Glycyrrhiza uralensis roots(Gancao).Besides its function in COVID-19 treatment,Gancao is able to induce pseudoaldosteronism by inhibiting renal 11β-HSD2.Systemic and colon-luminal exposure to Gancao compounds were characterized in volunteers receiving LianhuaQingwen and by in vitro metabolism studies.Access of Gancao compounds to 11β-HSD2 was characterized using human/rat,in vitro transport,and plasma protein binding studies,while 11β-HSD2 inhibition was assessed using human kidney microsomes.LianhuaQingwen contained a total of 41 Gancao constituents(0.01-8.56μmol/day).Although glycyrrhizin(1),licorice saponin G2(2),and liquiritin/liquiritin apioside(21/22)were the major Gancao constituents in LianhuaQingwen,their poor intestinal absorption and access to colonic microbiota resulted in significant levels of their respective deglycosylated metabolites glycyrrhetic acid(8),24-hydroxyglycyrrhetic acid(M2_(D);a new Gancao metabolite),and liquiritigenin(27)in human plasma and feces after dosing.These circulating metabolites were glucuronized/sulfated in the liver and then excreted into bile.Hepatic oxidation of 8 also yielded M2_(D).Circulating 8 and M2D,having good membrane permeability,could access(via passive tubular reabsorption)and inhibit renal 11β-HSD2.Collectively,1 and 2 were metabolically activated to the pseudoaldosterogenic compounds 8 and M2_(D).This investigation,together with such investigations of other components,has implications for precisely defining therapeutic benefit of LianhuaQingwen and conditions for its safe use. | Xiao-fang Lan Olajide EOlaleye Jun-lan Lu Wei Yang Fei-fei Du Jun-ling Yang Chen Cheng Yan-hong Shi Feng-qing Wang Xue-shan Zeng Nan-nan Tian Pei-wei Liao Xuan Yu Fang Xu Ying-fei Li Hong-tao Wang Nai-xia Zhang Wei-wei Jia Chuan Li | 2021 | Acta Pharmacologica Sinica2021，42，12： | 6 |
| 16 | Influences of accumulated mileage and technological changes on emissions of regulated pollutants from gasoline passenger vehicles显示文摘In this study, the influences of accumulated mileage(deterioration) and technological changes(emission standards) on emission factors(EFs) of regulated pollutants(CO, HC, and NO_x) from gasoline passenger vehicles were investigated based on Inspection and Maintenance(I/M) data using the chassis dynamometer method. The accumulated mileage of passenger vehicles was significantly linearly correlated with vehicle age. For most cases,the average EFs of CO, HC and NO_x were significantly linearly correlated with accumulated mileage, indicating that emission deterioration had a significant impact on pollutant EFs.Implemented emission standards markedly influenced the EFs of regulated pollutants, and EFs markedly decreased with progressing emission standards. The present study also compared EFs of regulated pollutants between this study and the International vehicle emission(IVE) model, and marked differences in EFs were seen with variations in emission standards, vehicle types and accumulated mileage; NO_x EFs in this study were higher than in the IVE model. The results provide new insight into estimating regulated pollutant emissions using the IVE model. | Qingyu Zhang Juwang Fan Weidong Yang Fang Ying Zhen Bao Yi Sheng Cheng Lin Xuan Chen | 2018 | Journal of Environmental Sciences2018，30，9： | 5 |
| 17 | Dealumination and desilication for Al-rich HZSM-5 zeolite via steam-alkaline treatment and its application in methanol aromatization显示文摘The hierarchical HZSM-5 was prepared via dealumination and desilication of commercial Al-rich HZSM-5, and characterized by X-ray diffraction, 27Al magic-angle spinning nuclear magnetic resonance, inductively coupled plasma mass spectrometry, scanning electron microscope, transmission electron microscope, N2 adsorption-desorption, NH3 temperature-programmed desorption, performed thermogravimetric and Raman spectrum. The results showed that partial framework of HZSM-5 was removed after steam treatment at 0.15 MPa, 500℃ for 3 h. HZSM-5 with high specific surface area and much mesoporosity was obtained by the subsequent alkaline treatment. The regulation of acid quantity was achieved by altering the concentration of alkaline. Dealumination and desilication of Al-rich HZSM-5 zeolites became more effective using a combination of steam and alkaline treatments than using alkaline treatment alone. Methanol aromatization reaction was employed to evaluate the catalytic performance of treated HZSM-5 at 0.15 MPa, 450℃ and MHSV of 1.5 h^-1. The results indicated that after steam treatment, HZSM-5 further treated with 0.2 mol/L NaOH exhibits the best catalytic performance: the selectivity of aromatics reached 42.1% and the lifetime of catalyst attained 212 h, which are much better than untreated HZSM-5. | Yuehua Fang Fan Yang Xuan He Xuedong Zhu | 2019 | Frontiers of Chemical Science and Engineering2019，13，3： | 5 |
| 18 | Tumor-targeting efficacy of a BF211 prodrug through hydrolysis by fibroblast activation protein-α显示文摘BF211, bufalin (BF ) 衍生物,比 BF,但是与潜在的 cardiotoxicity 展出更强壮的反癌症活动。成纤维细胞激活蛋白质 --(FAP ) 膜界限朊酶被联系癌的成纤维细胞明确地表示,因此被用于 anticancer 代理人的选择交货。在这研究,我们使用了基于 FAP 的 prodrug 策略综合 dipeptide (Z-Gly-Pro ) 结合了 BF211 prodrug 命名 BF211-03。BF211-03 是由 recombinant 人 FAP (rhFAP ) 的 hydrolyzed 并且由人的结肠癌 HCT-116 或人的胃的癌症 MGC-803 异种皮移植的 homogenates 劈开。相反, BF211-03 在血浆并且在 FAP 否定的正常纸巾的 homogenates 显示出好稳定性,例如心和肾。在有 FAP 表示的底层的 HCT-116 和 MGC-803 房间, BF211-03 显示了一在比到40褶层的有约 30 的 BF211 ,更大的 IC 50珍视的 vitro cytotoxicity 更低,而在有 FAP 的高水平的人的乳癌 MDA-MB-435 房间表达式, BF211-03 和 BF211 之间的 IC 50值差别是小的(近似4褶层)。尽管对肿瘤房间的 BF211-03 的 cytotoxicity 被化学装饰戏剧性地减少,它被 rhFAP 或肿瘤 homogenate 在 BF211-03 的劈开以后恢复。在 HCT-116 忍受肿瘤的裸体老鼠,加倍 BF211-03 的剂量,与肿瘤生长上的 BF211,引起的更少重量损失,而是出现类似的禁止的效果相比。我们的结果建议 BF211-03 在忍受肿瘤的裸体老鼠在肿瘤纸巾和展览反肿瘤活动被变换成活跃 BF211。指向 FAP 的 BF211-03 显示肿瘤选择并且可能作为一个指向的代理人有用在癌症为使用改进细胞毒素的自然产品的安全侧面治疗。 | Xiao-ping CHAI Guang-long SUN Yan-fen FANG Li-hong HU Xuan LIU Xiong-wen ZHANG | 2018 | Acta Pharmacologica Sinica2018，39，3： | 5 |
| 19 | Senegenin promotes in vitro proliferation of human neural progenitor cells显示文摘Senegenin,an effective component of Polygala tenuifolia root extract,promotes proliferation and differentiation of neural progenitor cells in the hippocampus.However,the effects of senegenin on mesencephalon-derived neural progenitor cells remain poorly understood.Cells from a ventral mesencephalon neural progenitor cell line(ReNcell VM) were utilized as models for pharmaceutical screening.The effects of various senegenin concentrations on cell proliferation were analyzed,demonstrating that high senegenin concentrations(5,10,50,and 100 μmo/L),particularly 50 μmol/L,significantly promoted proliferation of ReNcell VM cells.In the mitogen-activated protein kinase signal transduction pathway,senegenin significantly increased phosphorylation levels of extracellular signal-regulated kinases.Moreover,cell proliferation was suppressed by extracellular signal-regulated kinase inhibitors.Results suggested that senegenin contributed to in vitro proliferation of human neural progenitor cells by upregulating phosphorylation of extracellular signal-regulated kinase. | Shi, Fang Liang, Zhigang Guo, Zixuan Li, Ran Yu, Fen Zhang, Zhanjun Wang, Xuan Wang, Xiaomin | 2011 | Neural Regeneration Research2011，6，3： | 5 |
| 20 | Garnet granulite facies xenoliths from Yingfengling Cenozoic basalt in Leizhou, Guangdong Province显示文摘The granulite xenoliths are first found in Yingfengling pyroclastic rocks of Leizhou region, Guangdong Province. Of them high_pressure garnet granulite xenolith found is very sparse in China. Garnet granulite is different from pyroxene granulite in mineral assemblage and composition. \%P_T\% calculation shows that garnet granulite was formed at 1 130-1 160℃and 1.4-1.7 GPa, and pyriclasite at about 800℃and 0.65-0.80 GPa. High xenolith_derived paleogeotherm indicates Cenozoic rifting in Leizhou area. Granulites with varied mineral assemblages were formed at different depths by the metamorphism of the underplated basaltic melt. | Jinhai Yu Zhong Fang Xinmin Zhou Minyuan Lai Xisheng Xu Xuan Zhou | 1998 | Chinese Science Bulletin1998，43，23： | 5 |
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