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| 1 | Hydrodynamic Gene Delivery of Interleukin-22 Protects the Mouse Liver from Concanavalin A-, Carbon Tetrachloride-,and Fas Ligand-Induced Injury via Activation of STAT3显示文摘Interleukin-22 (IL-22) is a recently identified T cell-derived cytokine whose biological significance remains obscure. Previously, we have shown that IL-22 plays a protective role in T cell-mediated hepatitis induced by Concanavalin A (Con A), acting as a survival factor for hepatocytes. In the present paper, we demonstrate that hydrodynamic gene delivery of IL-22 cDNA driven either by a liver-specific albumin promoter or a human cytomegalovirus (CMV) promoter results in IL-22 protein expression, STAT3 activation, and expression of several anti-apoptotic proteins, including Bcl-xL, Bcl-2, and Mcl-1 in the liver. Immunohistochemical analysis reveals that IL-22 protein expression is mainly detected in the cytoplasm of hepatocytes. Overexpression of IL-22 by hydrodynamic gene delivery significantly protects against liver injury, necrosis, and apoptosis induced by administration of Con A, carbon tetrachloride (CCl4), or the Fas agonist Jo-2 mAb. Western blot analyses show that overexpression of IL-22 significantly enhances activation of STAT3 and expression of Bcl-xL, Bcl-2,and Mcl-1 proteins in liver injury induced by Con A. In conclusion, hydrodynamic gene delivery of IL-22 protects against liver injury induced by a variety of toxins, suggesting the therapeutic potential of IL-22 in treating human liver disease. Cellular & Molecular Immunology. 2004;1(1):43-49. | HongnaPan FengHong SvetlanaRadaeva BinGao | 2004 | Cellular & Molecular Immunology2004，1，1： | 30 |
| 2 | Hepatitis B Virus X Protein Sensitizes Primary Mouse Hepatocytes to Ethanol-and TNF-α-Induced Apoptosis by a Caspase-3-Dependent Mechanism显示文摘It is well-documented that alcohol drinking together with hepatitis viral infection accelerates liver injury; however the underlying mechanisms remain unknown. In this paper, we demonstrated that primary hepatocytes from transgenic mice overexpressing hepatitis B virus X protein (HBX) were more susceptible to ethanol- and TNF-α-induced apoptotic killing. Compared to normal control mouse hepatocytes, ethanol and/or TNF-α treatment led to a significant increase in reactive oxygen species, mitochondrial permeability transition, cytochrome C release,caspase-3 activity, and poly (ADP-ribose) polymerase degradation in hepatocytes from HBX transgenic mice.Blocking caspase-3 activity antagonized ethanol- and TNF-α-induced apoptosis in primary hepatocytes from HBX transgenic mice. Taken together, our findings suggest that HBX sensitizes primary mouse hepatocytes to ethanoland TNF-α-induced apoptosis by a caspase-3-dependent mechanism, which may partly explain the synergistic effects of alcohol consumption and hepatitis B virus infection on liver injury. | Won-HoKim FengHong BarbaraJaruga ZhengshengZhang SaijunFan T.JakeLiang BinGao | 2005 | Cellular & Molecular Immunology2005，2，1： | 10 |
| 3 | 60 Years of Development of the Journal of Integrative Plant Biology显示文摘In celebration of JIPB's 60 th anniversary,this paper summarizes and reviews the development process of the journal.To start,we offer our heartfelt thanks to JIPB's pioneer Editors-in-Chief who helped get the journal off the ground and make it successful.Academic achievement is the soul of academic journals,and this paper summarizes JIPB's course of academic development by analyzing it in four stages:the first two stages are mostly qualitative analyses,and the latter two stages are dedicated to quantitative analyses.Most-cited papers were statistically analyzed.Improvements in editing,publication,distribution and online accessibility-which are detailed in this paper-contribute to JIPB's sustainable development.In addition,JIPB's evaluation index and awards are provided with accompanying pictures.At the end of the paper,JIPB's milestones are listed chronologically.We believe that JIPB's development,from a national journal to an international one,parallels the development of the Chinese plant sciences. | Jinzhong Cui Ping He Fenghong Liu Jingjing Tan Lingfeng Chen Joshua Fenn | 2012 | Journal of Integrative Plant Biology2012，54，10： | 5 |
| 4 | Arabidopsis Thylakoid Formation 1 Is a Critical Regulator for Dynamics of PSII-LHCII Complexes in Leaf Senescence and Excess Light显示文摘在更高的植物, photosystem II (PSII ) 是超分子的建筑群 PSII 核心建筑群和植物特定的外部轻收获的建筑群(LHCII ) 填写了的大 pigmentprotein。PSIILHCII 建筑群在他们的数量和宏组织是高度有活力的到各种各样的环境条件。在这研究,我们报导了一个关键因素, Arabidopsis Thylakoid 形成 1 (THF1 ) 蛋白质,它在导致黑暗的老朽和轻环境适应期间控制 PSIILHCII 动力学。在 THF1 的 Loss-of-function 变化在感染病原体、衰老的叶子导致停留绿色显型。LHCII 和 PSII 核心子单元在 thf1 的导致黑暗的衰老的叶子被保留, PSIILHCII 建筑群的存在的陈述语气。当 PSIILHCII supercomplexes 的稳定性显著地减少了时,蓝土著人(BN )-polyacrylamide 胶化电气泳动(页) 和 immunoblot 分析证明在 dark-light-treated 和 high-light-treated thf1 叶子, PSIILHCII megacomplex 的一种类型有选择地被保留,建议在 PSIILHCII 建筑群的动力学的 THF1 的一个双角色。我们进一步证明 THF1 以一种 pH 依赖的方式与 Lhcb 蛋白质交往并且 thf1 的停留绿色显型依靠 LHCII 建筑群的存在。一起拿,数据建议 THF1 在更高的植物为 PSIILHCII 超分子的组织的动力学被要求。 | Weihua Huang Qingbo Chen Ying Zhu Fenghong Hu Lingang Zhang Zhaoxue Ma Zuhua He Jirong Huang | 2013 | Molecular Plant2013，6，5： | 4 |
| 5 | Biodiesel Production from Waste Edible Oils and Grease Containing Free Fatty Acids显示文摘Till now, most part of the biodiesel is produced from the refined vegetable oils using methanol as feedstock in the presence of an alkali catalyst. However, large amount of waste edible oils and grease are available. The difficulty with alkali-catalyzed esterification of these oils is that they often contain large amount of free fatty acids (FFA), polymers and decomposition products. These free fatty acids can quickly react with the alkali catalyst to produce soaps that inhibit the separation of the ester and glycerine. An esterification and transesterification process is developed to convert the high FFA oil to its monoesters. The first step, the acidcatalyzed esterification with glycerine and these FFA reduces the FFA content of the oil and grease to less than3%, and then an azeotropic distillation solvent is used to remove the water. The major factors affecting the conversion efficiency of the process such as glycerol to free fatty acid molar ratio, catalyst amount, reaction temperature and reaction duration are analyzed. The second step, alkali-catalyzed transesterification process converts the products of the first step to its monoesters and glycerol, and then the glycerol is recycled for utilization in the first step. Technical indicators of the biodiesel product can meet the ASTM 6751 standard. | Huang Fenghong Guo Pingmei Huang Qingde | 2005 | China Petroleum Processing & Petrochemical Technology2005，7，3： | 3 |
| 6 | A Study on Triacylglycerol Composition and the Structure of High-Oleic Rapeseed Oil显示文摘 | Mei Guan Hong Chen Xinghua Xiong Xin Lu Xun Li Fenghong Huang Chunyun Guan | 2016 | Engineering2016，2，2： | 3 |
| 7 | Surfactant-Encapsulated Polyoxometalate Complex as a Cathode Interlayer for Nonfullerene Polymer Solar Cells显示文摘An alcohol-soluble,environmentally friendly,and low-cost surfactant-encapsulated polyoxometalate complex[(C8H17)4N]4[SiW_(12)O40](TOASiW_(12))as a cathode interlayer(CIL)has exhibited excellent universality for various active layers and cathodes in nonfullerene polymer solar cells(NF-PSCs).In particular,incorporating TOASiW_(12) as the CIL enhanced power conversion efficiencies(PCEs)of the PM6:Y6-based NF-PSCs with Al or Ag cathode to 16.14%and 15.89%,respectively,and the PCEs of PM6:BTP-BO4Cl-based NF-PSCs with Al or Ag cathode to 17.04%and 17.00%,respectively.More importantly,the performances of the devices with TOASiW_(12) were insensitive to the TOASiW_(12) thickness from 3 to 33 nm.Furthermore,the NF-PSCs with TOASiW_(12) exhibited better device stability.Combined characterization of the photocurrent density versus effective voltage,capacitance versus voltage and electron mobility demonstrated that TOASiW_(12) as the CIL effectively promoted exciton dissociation,charge-carrier extraction,built-in potential,charge-carrier density,and electron mobility in the NF-PSCs.These findings suggest that TOASiW_(12) is a promising,competitive CIL for NF-PSCs fabricated by roll-to-roll processing. | Jing Qiu Yue Zhang Yan Liu Huiru Liu Dongdong Xia Fan Yang Chaowei Zhao Weiwei Li Lixin Wu Fenghong Li | 2022 | CCS Chemistry2022，4，3： | 3 |
| 8 | Free fatty acids profiling in cold-pressed rapeseed oil pretreated by microwave显示文摘This study proposed a strategy based on simple chemical derivatization coupled with neutral loss scan tandem to electrospray ionization source of mass spectrometer (NLS-ESI-MS/MS) for rapid and sensitive detection of free fatty acids (FFAs) in coldpressed rapeseed oils without sample purification and enrichment. The method was applied to investigate contents of FFAs and their changes in cold-pressed rapeseed oil pretreated by microwave. Results showed that main FFAs in rapeseed oil control samples were myristic acid (C14:0), palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2) and linolenic acid (C18:3). The highest content of C18:1 was 7163.69 nmol/g and C18:2 was 5221.18 nmol/g among 8 oil samples. FFAs generally increased after microwave pretreatment except C14:0 in oil sample of ‘Xinyou 17’ and ‘Suyou 6’. C18:1 and C18:2 significantly changed (P<0.05) after microwave pretreatment. With this established simple,practical and reliable analysis approach, changes of FFAs would be monitored to ensure safety and quality of edible oils. A powerful tool is provided for thermal degradation mechanism research of edible oils. | Ming Liu Chang Zheng Pingmei Guo Xuyan Dong Fang Wei Hong Chen Fenghong Huang | 2017 | Oil Crop Science2017，2，2： | 2 |
| 9 | The FAIR Principles:First Generation Implementation Choices and Challenges显示文摘“FAIR enough”?...A question asked on a daily basis in the rapidly evolving field of open science and the underpinning data stewardship profession.After the publication of the FAIR principles in 2016,they have sparked theoretical debates,but some communities have already begun to implement FAIR-guided data and services.No-one really argues against the idea that data,as well as the accompanying workflows and services should be findable,accessible under well-defined conditions,interoperable without data munging,and thus optimally reusable.Being FAIR is not a goal in itself;FAIR Data and Services are needed to enable data intensive research and innovation and(thus)have to be“AI-ready”(=future proof for machines to optimally assist us).However,the fact that science and innovation becomes increasingly“machine-assisted”and hence the central role of machines,is still overlooked in some cases when people claim to implement FAIR. | Barend Mons Erik Schultes Fenghong Liu Annika Jacobsen | 2020 | Data Intelligence2020，2，1： | 2 |
| 10 | Microencapsulation of nutmeg essential oil by spray-drying with different wall materials显示文摘Nutmeg essential oil with volatile components is sensitive to oxygen, light, water and high temperature. Spray-drying is widely used as an encapsulation technique for improving compound stability during processing and storage. In this study, influence of encapsulated matrix on spray-dried nutmeg essential oil microcapsule was investigated using blends of maltodextrin and either gum Arabic(GA-MD), or octenyl succinic acid modified starch(OSA-MD) as wall materials. Characteristics of spray-dried powders from these 2 wall materials were evaluated and compared. Powder property analysis indicated that GA-MD microcapsules presented larger size, higher moisture and poorer flowability compared with OSA-MD microcapsules. Fourier transforms infrared spectroscopy and thermogravimetric analysis showed that both GA-MD and OSA-MD microcapsules improved the stability of nutmeg essential oil. However, GA-MD microcapsules exhibited higher storage stability than OSA-MD microcapsules. This research provided theoretical foundation for wall material choice of microcapsule and nutmeg essential oil utilization in food industry. | Xuyan Dong Bangfu Wu Qianchun Deng Fang Wei Hong Chen Chen Yang Renhuai Cong Chayan Wan Xin Lv Fenghong Huang | 2018 | Oil Crop Science2018，3，4： | 2 |
| 11 | Modification of Hole Transport Layers for Fabricating High Performance Non-fullerene Polymer Solar Cells显示文摘Interfacial engineering is expected to be a feasible strategy to improve the charge transport properties of the hole transport layer(HTL),which is of crucial importance to boost the device performance of organic solar cells(OSCs).In this study,two types of alcohol soluble materials,2,3,5,6-tetrafluoro-7,7,8,8-tetracyanoquinodimethane(F4-TCNQ)and di-tetrabutylammoniumcis-bis(isothiocyanato)bis(2,2'-bipyridyl-4,4'-dicarboxylato)ruthenium(ll)(N719)dye were selected as the dopant for HTL.The doping of F4-TCNQ and N719 dye in poly(ethylenedioxythiophene):poly(styrene sulfonate)(PEDOT:PSS)with and without integrating a graphene quantum-dots(G-QDs)layer has been explored in poly[[2,6'-4-8-di(5-ethylhexylthienyl)benzo[1,2-b:3,3-b]dithiophene][3-fluoro-2[(2-ethylhexyl)carbonyl]thieno[3,4-b]thio-phenediyl:(2,2'-((2Z,2'Z)-(((4,4,9,9-tetrakis(4-hexylphenyl)-4,9-dihydro-s-indaceno[1,2-b:5,6-b']dithiophene-2,7-diyl)bis(4-((2-ethylhexyl)oxy)thiophene-5,2-diyl))bis(methanylylidene))bis(5,6-difluoro-3-oxo-2,3-dihydro-1H-indene-2,1-diylidene))dimalononitrile(PTB7-Th:IEICO-4F)OSCs.The power conversion efficiency of the non-fullerene OSCs has been increased to 10.12%from 8.84%.The influence of HTL modification on the nano-morphological structures and photophysical properties is analyzed based on the comparative studies performed on the control and modified devices.The use of chemical doping and bilayer strategy optimizes the energy level alignment,nanomorphology,hole mobility,and work-function of HTL,leading to considerable reduction of the leakage current and recombination losses.Our work demonstrates that the doping of HTL and the incorporation of G-QDs layer to constitute a bilayer HTL is an promising strategy to fabricate high performance non-fullerene polymer solar cells. | B.Hari Babu Chengkun Lyu Hongwei Zhang Zhihao Chen Fenghong Li Lin Feng Xiao-Tao Hao | 2020 | Chinese Journal of Chemistry2020，38，8： | 2 |
| 12 | Physicochemical properties and volatile components of hempseed oils in Bama region显示文摘A comparison study has been performed in hempseed oils (HSO) extracted from different cultivars in Bama region. Squeezing properties, chemical compositions, thermal stability and flavor profile of pressed HSO were determined in this study. Results indicated that there were no significant differences in crude fat, protein, fiber of different hempseeds and fatty acid composition in HSOs. Hempseeds with small size and dark-colored peel have lower squeezing oil yield than big ones. Average content of total phytosterol, tocopherol and phenol in HSO from small seeds were 872.0 mg/100 g, 124.1 mg/100 g and 15.5 mg SA/100 g respectively. Thermal oxidation by differential scanning calorimetry (DSC) revealed three step oxidation of HSO with mean onset and oxidation temperature at 124oC and 135-315oC. Oxidation temperature of HSO from small seeds was higher than that from big seeds (big size and light-colored peel). Main volatile ingredients in HSO contained 11 kinds of monoterpene and 7 kinds of sesquiterpene. Volatile terpene content in HSO from small seeds was nearly 30% higher than that of big seeds. Quality of HSO with different peel feature has significant difference in squeezing yield, thermal stability and volatile flavors. This work may be useful to produce high quality hempseed oils through quality evaluation. | Qi Zhou Fenghong Huang Chang Zheng Pingmei Guo Wenlin Li Changsheng Liu Chuyun Wan | 2017 | Oil Crop Science2017，2，1： | 2 |
| 13 | Epidemiology and prevention of hepatitis B virus in China显示文摘Hepatitis B virus (HBV) infection is prevalent in China. Approximately 600 million people have ever been infected by HBV. About 130 million are HBV chronic carriers and 30 million HB patients. Among them, 50% of HBV carriers are caused by carrier mothers to born infants. Around 300 000 people died of liver disease including liver cirrhosis and primary hepatocellular carcinoma each year and 50% of them died of primary hepatocellular carcinoma. HBV infection is not only the health problem but also becoming a social problem. HBV chronic carriers and patients have endured the great pressure from disease burden and social discrimination. According to the report of the national screening program of HBV released by the ministry of health in 2008, China has taken many effective measures to control the HBV infection, including vaccine immunization program, strengthening the management of blood sources and blood productions, prevention of nosocomial HBV infection, strengthening health education on HBV infection and safe injection techniques. The implementation of HB vaccine immunization program, which China officially introduced into the national immunization program since 1992, has dramatically reduced the incidence of HBV infection among infants and children. Integrated with other interventions, the rate of HBV infection decreased gradually. According to the survey of the national screening program of HBV in 2006, compared with the incidence of HBV in 1992, the incidence rate of HBsAg positive has decreased 26.36% , the number of children who have ever been infected by HBV decreased 80 million since 1992. However some problems are still existing. The solutions of low rate of vaccination in rural areas and migration population, lacking of practical measures on management of hepatitis B patients, the occurrence of health care acquired HBV infection, and low rate of vaccination among high risk groups have also been recommended. | Zhang Xinping Wang Fenghong | 2009 | Journal of Medical Colleges of PLA(China)2009，24，5： | 2 |
| 14 | Study on the protection of Er-doped phosphate glass waveguide surface in ion-exchange processing显示文摘A novel method, sputtering K9 glass film, is proposed to solve the surface corrosion of Er-doped phosphate glass during ion-exchange processing for optical waveguide fabrication. The corrosion causes are analyzed to be the intrinsically weak stabilization of phosphate glass structure, hydrophile and weakly acidic property of phosphate radical. Experimental results show that the K9 glass film could not only protect the Er-doped phosphate glass surface from being corroded but also give no influence on the waveguide fabrication. The effect of thickness of K9 glass film on the optical property of waveguide is also investigated and the op- timal thickness is found to be 60―80 nm. It provides a good base for further fabri- cation of active phosphate glass optical waveguide devices. | HAN XiuYou CHU FengHong CAI HaiWen FANG ZuJie ZHAO MingShan | 2008 | Science China(Technological Sciences)2008，51，7： | 2 |
| 15 | Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes显示文摘The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 μM arsenite for 24 hours. Cell viability and morphological observations revealed that 5 μM arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 μM arsenite for 24 hours and stimulated with 25 μM glutamate for 10 minutes. Results showed that [Ca2+]i in astrocytes exposed to 5 and 10 μM arsenite was significantly increased and levels of D-serine, γ-aminobutyric acid and glycine in cultures exposed to 2.5-10 μM arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 μM arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function. | Yan Wang Fenghong Zhao Yingjun Liao Yaping Jin Guifan Sun | 2012 | Neural Regeneration Research2012，7，31： | 1 |
| 16 | Impact of sesame lignan on physical and oxidative stability of flaxseed oil-in-water emulsion显示文摘Recent studies have shown that the highly susceptibility to oxidation ofα-linolenic acid(ALA)enriched emulsion delivery system was harmful for human health which limited their incorporation into functional food.Impacts of natural sesamol(SOH)and sesamin(SES)on stability of flaxseed oil-in-water emulsion were investigated.Results showed that SOH indicated higher antioxidant activity and significantly prolonged the time of emulsion oil-off by retarding oil droplet aggregation in a dose dependent manner throughout storage.Moreover,SOH showed substantial extended lag phase of lipid oxidation products,especially for secondary oxidation products(thiobarbituric acid-reactive substances,TBARS),with a maximum reduction of 70%with 800 M dosage.The antioxidative efficiency of SOH might relate to its strong ability of scavenging free radical and chelate transition metal.Furthermore,SOH significantly enhanced interfacial barrier property and reduced permeation rate of peroxyl radical across emulsion interface by hydrogen bonds between sugar groups of saponin molecules and SOH.However,no obvious change in barrier property of emulsion was observed in SES.SOH improved physicochemical property of flaxseed oil-in-water emulsion with higher antioxidant activity and stronger interfacial barrier property,so that it could be serve as plant-based antioxidant in oil-in-water emulsion delivery system. | Xintian Wang Kun Yu Chen Cheng Xiao Yu David Julian McClements Wenwen Huang Jia Yang Fenghong Huang Qianchun Deng | 2019 | Oil Crop Science2019，4，4： | 1 |
| 17 | Electrochemical Route to Fabricate Film‐Like Conjugated Microporous Polymers and Application for Organic Electronics显示文摘 | Cheng Gu Youchun Chen Zhongbo Zhang Shanfeng Xue Shuheng Sun Kai Zhang Chengmei Zhong Huanhuan Zhang Yuyu Pan Ying Lv Yanqin Yang Fenghong Li Suobo Zhang Fei Huang Yuguang Ma | 2013 | Adv Mater2013，，25： | 1 |
| 18 | Magnetic solid base catalysts for the production of biodiesel 显示文摘 | Guo Pingmei Huang Fenghong Zheng Mingming | 2012 | J Am Oil Chem Soc2012，89，： | 1 |
| 19 | Performances of super-long span prestressed cable-stayed bridge with CFRP cables and RPC girder显示文摘To discuss the applicability of advanced composite carbon fiber reinforced polymer(CFRP) and ultrahigh performance concrete reactive powder concrete(RPC) in super-long span cable-stayed bridges, taking a 1 008 m cable-stayed bridge with steel girders and steel cables as an example,a new cable-stayed bridge in the same span with RPC girders and CFRP cables was designed,in which the cable's cross section was determined by the principle of equivalent cable capacity and the girder's cross section was determined in virtual of its stiffness, shear capacity and local stability. Based on the methods of finite element analysis,the comparative analysis of these two cable-stayed bridge schemes about static performances,dynamic performances,stability and wind resistance behavior were carried out. The results showed that it was feasible to form a highly efficient,durable concrete cable-stayed bridge with RPC girders and CFRP cables and made its applicable span range expand to 1 000 m long around. | Fang Zhi Fan Fenghong Ren Liang | 2013 | Engineering Sciences2013，11，5： | 1 |
| 20 | Cellular antioxidant activity and cytotoxicity assay of canolol显示文摘To better understand canolol as an efficient intracellular antioxidant agent in food related fields, potential cellular antioxidant activity and cytotoxicity were evaluated using multiple assays, including cellular antioxidant activity(CAA), 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT) and methylene blue assay. CAA assay was used to identify the half maximal effective concentration of canolol and other bioactive components, such as sinapic acid, resveratrol and sesamol in oil crops. Results showed that antioxidant capacity(without PBS wash) varies between different compounds. Capacity variation followed the order: sinapic acid > canolol > resveratrol > sesamol. While with PBS wash, the order changed to sinapic acid > resveratrol > canolol > sesamol. Half maximal inhibitory concentration of canolol measured by MTT was 1887.08±1.22 μmol/L, implying that cytotoxicity of canolol was greatly lower than that of resveratrol, sesamol and sinapic acid. Methylene blue assay showed that cell viability was still up to 90% exposed to canolol at a high concentration of 4444 μmol/L. It suggested that canolol had desired antioxidant activity in organism with low cellular cytotoxicity. | Xiaoyang Xia Xia Xiang Fenghong Huang Ling Han Zhen Zhang Mingming Zheng Chang Zheng | 2018 | Oil Crop Science2018，3，2： | 1 |
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