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| 1 | Effect of process parameters on additive-free electrospinning of regenerated silk fibroin nonwovens显示文摘Silk fibroin is a biomaterial with multiple beneficial properties for use in regenerative medicine and tissue engineering.When dissolving and processing the reconstituted silk fibroin solution by electrospinning,the arrangement and size of fibers can be manifold varied and according fiber diameters reduced to the nanometer range.Such nonwovens show high porosity as well as potential biocompatibility.Usually,electrospinning of most biomaterials demands for the application of additives,which enable stable electrospinning by adjusting viscosity,and are intended to evaporate during processing or to be washed out afterwards.However,the use of such additives increases costs and has to be taken into account in terms of biological risks when used for biomedical applications.In this study,we explored the possibilities of additive-free electrospinning of pure fibroin nonwovens and tried to optimize process parameters to enable stable processing.We used natural silk derived from the mulberry silkworm Bombyx mori.After degumming,the silk fibroin was dissolved and the viscosity of the spinning solution was controlled by partial evaporation of the initial solving agent.This way,we were able to completely avoid the use of additives and manufacture nonwovens,which potentially offer higher biocompatibility and reduced immunogenicity.Temperature and relative humidity during electrospinning were systematically varied(25–35°C,25–30%RH).In a second step,the nonwovens optionally underwent methanol treatment to initiate beta-sheet formation in order to increase structural integrity and strength.Comprehensive surface analysis on the different nonwovens was performed using scanning electron microscopy and supplemented by additional mechanical testing.Cytotoxicity was evaluated using BrdU-assay,XTT-assay,LDH-assay and live-dead staining.Our findings were,that an increase of temperature and relative humidity led to unequal fiber diameters and defective nonwovens.Resistance to penetration decreased accordingly.The most uniform fiber diameters of 998±63 nm were obtained at 30°C and 25%relative humidity,also showing the highest value for resistance to penetration(0.20 N).The according pure fibroin nonwoven also showed no signs of cytotoxicity.However,while the biological response showed statistical evidence,the material characteristics showed no statistically significant correlation to changes of the ambient conditions within the investigated ranges.We suggest that further experiments should explore additional ranges for temperature and humidity and further focus on the repeatability of material properties in dependency of suitable process windows. | Alexander Kopp Ralf Smeets Martin Gosau Nadja Kröger Sandra Fuest Marius Köpf Magnus Kruse Judith Krieger Rico Rutkowski Anders Henningsen Simon Burg | 2020 | Bioactive Materials2020，5，2： | 3 |
| 2 | Purification and characterization of a glutathione stransferase from benoxacortreated maize(Zea mays) 显示文摘 | Gerard P Irzy K Patrick Fuest E | 1993 | Plant Physiol1993，102，： | 1 |
| 3 | Flow Field Studies of a New Series Of Turbulent Premixed Stratified Flames 显示文摘 | Seffrin F Fuest F Geyer D | 2010 | Combustion and Flame2010，157，2： | 1 |
| 4 | Practical clinical pharmacology and drug interactions of low-dose methotrexate therapy in rheumatoid arthritis显示文摘 | Fuest DE | 1995 | Br J Bheumatol1995，34，2： | 1 |
| 5 | Nausea in cancer chemotherapy is inversely related to urinary cortisol excretion显示文摘 | Fredrikson M Hursti T Fuest J | 1992 | Br J Cancer1992，65，5： | 1 |
| 6 | EU Regional Policy and Tax Competition显示文摘 | Becker J Fuest C | 2010 | European Economic Review2010，54，1： | 1 |
| 7 | Taxing foreign profits with international mergers and acquisitions 显示文摘 | Becker J Fuest C | 2010 | International Economic Review2010，51，1： | 1 |
| 8 | The transcription factor c-Jun protects against sustained hepatic endoplasmic reticulum stress thereby promoting hepatocyte survival显示文摘 | Fuest M Willim K MacNelly S | | 0，，02： | 1 |
| 9 | 何为聚氨酯?用于哪些方面?显示文摘功能设计成功的关健当然是选择适当的材料,以满足应用所需。某些情况下,选择钢、铝或其它金属,而另一种情况下,塑料—ABC、聚苯乙烯、PVC、酚醛树脂或需求量增长的聚氨酯则是最好的材料。聚氨酯能提供优异的设计和良好的特性。 | Ronald W. Fuest 郭焕萍 | 1989 | 橡胶参考资料1989，，1： | 1 |
| 10 | Nitriding of Melt-spun Nd-Fe-Mo Alloy显示文摘 | Fuest CD Herbst JF | 1994 | J Appl Phys1994，75，10： | 1 |
| 11 | Risk hazard mapping of groundwater contamination using long-term monitoring data of shallow drinking water wells 显示文摘 | FUEST S BERLEKAMP J KLEIN M | 1998 | Journal of Hazardous Materials1998，61，123： | 1 |
| 12 | Nausea in cancer chemotherapy is inversely related to urinary cortisol excretion显示文摘 | Fredrikson M Hursti T Fuest J | 1992 | Br J Cancer1992，65，： | 1 |
| 13 | Source versus residence based taxation with international mergers and acquisitions显示文摘 | Becker J Fuest C | 2011 | Journal of Public E- conomics2011，95，1： | 1 |
| 14 | Nausea in cancer chemotherapy is inversely related to urinary cortisol excretion显示文摘 | Fredrikson M Hursti T Fuest J | 1992 | Br J Cancer1992，65，： | 1 |
| 15 | Tax competition--greenfield investment ver- sus mergers and acquisitions显示文摘 | Becker J Fuest C | 2011 | Regional Science and Urban Economics2011，41，5： | 1 |
| 16 | Imaging of P- glycoprotein-mediated pharmacoresistance in the hippocampus: proof-of-concept in a chronic rat model of temporal lobe epilepsy 显示文摘 | Bartmann H Fuest C la Fougere C | 2010 | Epilepsia2010，51，9： | 1 |
| 17 | Pyrrolidine dithiocarbamate protects the piriform cortex in the pilocarpine status epilepticus model显示文摘 | Soerensen J Pekcec A Fuest C | 2009 | Epilepsy Res2009，87，23： | 1 |
| 18 | Practical clinical pharmacology and drug interactions of low-dose methotrexate therapy in rheumatoid arthritis 显示文摘 | Fuest DE | 1995 | Br J Rheumatol1995，34，2： | 1 |
| 19 | Tonom-etry in Corneal Edema after Cataract Surgery:Rebound ver- sus Goldmann Applanat Tonometry 显示文摘 | Matthias Fuest Nikolaos Mamas Peter Walter | 2014 | Current Eye Re- search2014，39，9： | 1 |
| 20 | Nausea in cancer chemotherapy is inversely related to urinary cortisol excretion 显示文摘 | FREDRIKSON M HURSTI T FUEST J | 1992 | Br J Cancer1992，65，5： | 1 |
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