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| 1 | Effect of cDNA fragments in different length derived from Potato Virus Y coat protein gene on the induction of RNA-mediated virus resistance显示文摘We have reported that cDNA derived from entire coat protein (CP) gene of potato virus Y (PVY) could induce resistance to PVY infection in transgenic tobacco plants, and the resistance was further demonstrated to be RNA-mediated rather than coat protein-mediated. In this study, we cloned cDNA fragments of 202 bp, 417 bp, and 603 bp in length derived from the 3′ end of the PVY CP gene, and the cDNA fragments were introduced into tobacco (var. NC89) plants via Agrobacterium-mediated transformation system. The results of resistance assay showed that the CP cDNA fragments of 417 bp and 603 bp could confer resistance of the trans-genic plants to PVY infection, but the fragment of 202 bp in length could not. Molecular analysis revealed that the resistance was RNA-mediated, which is believed to be a result of post-transcriptional gene silencing. The results indicate that the length of cDNA fragments needed for resistance induction was located somewhere between 202 bp and 417 bp from the 3′ end of PVY CP gene. | ZHU Junhua ZHU Xiaoping WEN Fujiang BAI Qingrong ZHU Changxiang SONG Yunzhi | 2004 | Science China(Life Sciences)2004，47，4： | 5 |
| 2 | Overexpression of phospholipase Dα gene enhances drought and salt tolerance of Populus tomentosa显示文摘The cDNA of AtPLDα (Arabidopsis thaliana Phospholipase Dα) gene was introduced into P. tomentosa (Populus tomentosa) under the control of the Cauliflower mosaic virus 35S promoter. Southern and Northern blot analyses suggested that the AtPLDα gene has been transferred into the P. tomentosa genome. No obvious morphological or developmental difference was observed between the transgenic and wild-type (WT) plants. Drought and salt tolerance and gene expression of seedlings of several transgenic lines and WT plants (control) were studied. The results showed that the rhizogenesis rate and the average root-length of transgenic lines were significantly higher than WT plants after mannitol and NaCl treatment under the same growth conditions. Northern blot analysis indicated that the higher the PLDα expression in the transgenic plants, the more tolerant the transgenic plants are to drought and salt treatment. Meanwhile, another group of these transgenic lines and WT plants (control) were treated with PEG6000 and NaCl separately. The contents of chlorophylls and the activities of some anti-oxidant enzymes (superoxide dismutase, guaiacol peroxidase and catalase) as well as malondialde-hyde and relative electrical conductivity were analyzed. Altogether, our results demonstrated that overexpression of the PLDα gene can enhance the drought and salt tolerance in transgenic P. tomen-tosa plants. | ZHANG TingTing SONG YunZhi LIU YuDong GUO XingQi ZHU ChangXiang WEN FuJiang | 2008 | Chinese Science Bulletin2008，53，23： | 3 |
| 3 | Research on Hybrid Model of Garlic Short-term Price Forecasting based on Big Data显示文摘Garlic prices fluctuate dramatically in recent years and it is very difficult to predict garlic prices.The autoregressive integrated moving average(ARIMA)model is currently the most important method for predicting garlic prices.However,the ARIMA model can only predict the linear part of the garlic prices,and cannot predict its nonlinear part.Therefore,it is urgent to adopt a method to analyze the nonlinear characteristics of garlic prices.After comparing the advantages and disadvantages of several major prediction models which used to forecast nonlinear time series,using support vector machine(SVM)model to predict the nonlinear part of garlic prices and establish ARIMA-SVM hybrid forecast model to predict garlic prices.The monthly average price data of garlic in 2010-2017 was used to test the effect of ARIMA model,SVM model and ARIMA-SVM model.The experimental results show that:(1)Garlic price is affected by many factors but the most is the supply and demand relationship;(2)The SVM model has a good effect in dealing with the nonlinear relationship of garlic prices;(3)The ARIMA-SVM hybrid model is better than the single ARIMA model and SVM model on the accuracy of garlic price prediction,it can be used as an effective method to predict the short-term price of garlic. | Baojia Wang Pingzeng Liu Zhang Chao Wang Junmei Weijie Chen Ning Cao Gregory MPO’Hare Fujiang Wen | 2018 | Computers, Materials & Continua2018，，11： | 3 |
| 4 | Isolation of a strong matrix attachment region (MAR) and identification of its function in vitro and in vivo显示文摘Inclusion of MARs in transgene cassettes enhances their expression and reduces position-effect variations in the transgenic host. Four new MARs (TM2, TM3, AM1 and AM2) were isolated from tobacco and Arabidopsis by PCR method. The nuclei isolated from suspension-cultured cells of rice were used to prepare nuclear matrix. With a characterized MAR (TM1) as a positive control, the Matrix-MAR interactions were tested by an in vitro binding assay to identify the DNA sequences as MARs and their binding strength to nuclear matrix in vitro was compared. The results showed that TM2 and TM3 had stronger binding strength than TM1. To determine the functions of the four new MARs in vivo, binary vectors pBI121 carrying a uidA GUS reporter gene were modified with direct repeat MARs inserted on both sides of the reporter gene cassette and were transferred into tobaccos via Agrobacterium-mediated transformation procedure. Quantitative GUS assays of the transgenic tobaccos showed that when flanking a GUS reporter gene | Kewei Zhang Jianmei Wang Guodong Yang Xingqi Guo Fujiang Wen Decai Cui Chengchao Zheng | 2002 | Chinese Science Bulletin2002，47，23： | 2 |
| 5 | Inheritance of gusA and neo genes in transgenic rice显示文摘 | Jianying Peng Fujiang Wen Richard L. Lister Thomas K. Hodges | 1995 | Plant Molecular Biology1995，，1： | 1 |
| 6 | Production of double-stranded RNA for interference with TMV infection utilizing a bacterial prokaryotic expression system显示文摘 | Guohua Yin Zhaonan Sun Nan Liu Lin Zhang Yunzhi Song Changxiang Zhu Fujiang Wen | 2009 | Applied Microbiology and Biotechnology2009，，2： | 1 |
| 7 | Research on the Relationship Between Garlic and Young GarlicShoot Based on Big Data显示文摘In view of the problems such as frequent fluctuation of garlic price, lack ofefficient forecasting means and difficulty in realizing the steady development of garlicindustry, combined with the current situation of garlic industry and the collected datainformation. Taking Big Data platform of garlic industry chain as the core, using themethods of correlation analysis, smoothness test, co-integration test, and Grangercausality test, this paper analyzes the correlation, dynamic, and causality between garlicprice and young garlic shoot price. According to the current situation of garlic industry,the garlic industry service based on Big Data is put forward. It is concluded that there is apositive correlation between garlic price and young garlic shoot price, and there is a longtermstable dynamic equilibrium relationship between young garlic shoot price and garlicprice fluctuation, and young garlic shoot price can affect garlic price. Finally, it isproposed to strengthen the infrastructure construction of garlic Big Data, increase thetechnological innovation and application of garlic Big Data technology, and promote thesafety and security ability of the whole industry to promote the development of garlicindustry. | Feng Guo Pingzeng Liu Wanming Ren Ning Cao Chao Zhang Fujiang Wen Helen Min Zhou | 2019 | Computers, Materials & Continua2019，，2： | 1 |
| 8 | Production of double-stranded RNA for interference with TMV infection utilizing a bacterial prokaryotic expression system显示文摘 | Guohua Yin Zhaonan Sun Nan Liu Lin Zhang Yunzhi Song Changxiang Zhu Fujiang Wen | 2009 | Applied Microbiology and Biotechnology2009，，2： | 1 |
| 9 | Towards sustainable protection against insect-borne plant viral diseases:Phytohormones and beyond显示文摘Due to the immobility of plants,around 75% of 1100 plant virus species are piercing-sucking insect transmitted.Host plant-mediated interactions between viruses and insects play vital roles in the population dynamics of vectors and the epidemiology of plant diseases.A successful viral pathogen has to evolve multiple strategies to manipulate host immune responses and also the ecological environment to facilitate effective transmission by insect vectors.Among these strategies,reprogramming the phytohormone signaling pathways is critical to the establishment of an effective virus transmission among plants and disease pandemic.Here,we review recent studies on the plant-virus inter-relationships with a focus on molecular and biochemical mechanisms that drive vector-borne viral diseases.Defense-related phytohormones such as Jasmonic acid(JA),Salicylic acid(SA)and Ethylene(ET)have been reported to be directly regulated by viral proteins.This knowledge is essential for the further design and/or development of effective and sustainable strategies to protect viral damages so as to increase crop yield and food security.Future efforts in this area should be focused on integrating and meta-analysis of big data generating from dynamics and multiple dimensional pathogen-vector-crop interactions under real agricultural conditions to achieve sustainable protection against plant diseases. | WANG Ning YAO XiangMei ZHU ChangXiang WEN FuJiang YE Jian | 2016 | Science Foundation in China2016，24，2： | 0 |
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