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| 1 | Analysis of the factors affecting the magnetic characteristics of nano-Fe_3O_4 particles显示文摘We prepared Fe3O4 nanoparticles using chemical coprecipitation and studied the factors affecting the magnetic characteristics of nano-Fe3O4 particles.We identified four factors and three levels of an orthogonal experiment and investigated these four factors that affect the magnetic characteristics of the Fe3O4 particles.We obtained important information from this investigation.The Fe3+ to Fe2+ molar ratio,the iron precursor salt,the amount of surfactant and the amount of alkali were found to be important.We also studied the influence of the order of alkali and surfactant addition,the aging time and the stirring speed on the magnetic characteristics of the nano-Fe3O4 particles.The Fe3O4 preparation process was also analyzed. | LI JianLing LI DeCai ZHANG ShaoLan CUI HongChao WANG Cui | 2011 | Chinese Science Bulletin2011，56，8： | 2 |
| 2 | Investigating the bacterial community and amoebae population in rural domestic wastewater reclamation for irrigation显示文摘Reclamation of domestic wastewater for agricultural irrigation is viewed as a sustainable option to create an alternative water source and address water scarcity. Free-living amoebae(FLA), which are amphizoic protozoa, are widely distributed in various environmental sources. The FLA could cause considerable environmental and health risks. However, little information is available on the risk of these protozoa. In this study, we evaluated the feasibility using rural domestic wastewater for agricultural irrigation, and analyzed dynamic changes of the microbial community structure and FLA populations in raw and treated wastewater, as well as the phyllosphere and rhizosphere of lettuce production sites that were irrigated with different water sources. The bacterial community dynamics were analyzed by terminal restriction fragment length polymorphism(T-RFLP). The bacterial community structures in the influent were similar to that in the effluent, while in some cases relative abundances varied significantly. The populations of Acanthamoeba spp. and Hartmannella vermiformis in the anaerobically treated wastewater were significantly higher than in the raw wastewater. The vegetables could harbor diverse amoebae, and the abundances of Acanthamoeba spp. and H. vermiformis in the rhizosphere were significantly higher than in the phyllosphere. Accordingly, our studies show insight into the distribution and dissemination of amoebae in wastewater treatment and irrigation practices. | Bingjian Cui Jinxue Luo Decai Jin Bo Jin Xuliang Zhuang Zhihui Bai | 2018 | Journal of Environmental Sciences2018，30，8： | 2 |
| 3 | Isolation of a strong matrix attachment region (MAR) and identification of its function in vitro and in vivo显示文摘Inclusion of MARs in transgene cassettes enhances their expression and reduces position-effect variations in the transgenic host. Four new MARs (TM2, TM3, AM1 and AM2) were isolated from tobacco and Arabidopsis by PCR method. The nuclei isolated from suspension-cultured cells of rice were used to prepare nuclear matrix. With a characterized MAR (TM1) as a positive control, the Matrix-MAR interactions were tested by an in vitro binding assay to identify the DNA sequences as MARs and their binding strength to nuclear matrix in vitro was compared. The results showed that TM2 and TM3 had stronger binding strength than TM1. To determine the functions of the four new MARs in vivo, binary vectors pBI121 carrying a uidA GUS reporter gene were modified with direct repeat MARs inserted on both sides of the reporter gene cassette and were transferred into tobaccos via Agrobacterium-mediated transformation procedure. Quantitative GUS assays of the transgenic tobaccos showed that when flanking a GUS reporter gene | Kewei Zhang Jianmei Wang Guodong Yang Xingqi Guo Fujiang Wen Decai Cui Chengchao Zheng | 2002 | Chinese Science Bulletin2002，47，23： | 2 |
| 4 | An efficient papaya leaf distortion mosaic potyvirus vector for virus-induced gene silencing in papaya显示文摘Papaya(Carica papaya L.)is regarded as an excellent model for genomic studies of tropical trees because of its short generation time and its small genome that has been sequenced.However,functional genomic studies in papaya depend on laborious genetic transformations because no rapid tools exist for this species.Here,we developed a highly efficient virus-induced gene silencing(VIGS)vector for use in papaya by modifying an artificially attenuated infectious clone of papaya leaf distortion mosaic virus(PLDMV;genus:Potyvirus),PLDMV-E,into a stable Nimble Cloning(NC)-based PLDMV vector,pPLDMV-NC,in Escherichia coli.The target fragments for gene silencing can easily be cloned into pPLDMV-NC without multiple digestion and ligation steps.Using this PLDMV VIGS system,we silenced and characterized five endogenous genes in papaya,including two common VIGS marker genes,namely,phytoene desaturase,Mg-chelatase H subunit,putative GIBBERELLIN(GA)-INSENSITIVE DWARF1A and 1B encoding GA receptors;and the cytochrome P450 gene CYP83B1,which encodes a key enzyme involved in benzylglucosinolate biosynthesis.The results demonstrate that our newly developed PLDMV VIGS vector is a rapid and convenient tool for functional genomic studies in papaya. | Decai Tuo Pu Yan Guangyuan Zhao Hongguang Cui Guopeng Zhu Yang Liu Xiukun Yang He Wang Xiaoying Li Wentao Shen Peng Zhou | 2021 | Horticulture Research2021，8，1： | 2 |
| 5 | Subcellular distribution and tissue expression of phospholipase Dα,Dβand Dγin Arabidopsis显示文摘 | Lu Fan Suqin Zheng Decai Cui | 1999 | Plant Physiology1999，119，： | 1 |
| 6 | Sequence-specific interactions of wound-inducible nuclear factors with mannopine synthase 2′ promoter wound-responsive elements显示文摘 | Min Ni Decai Cui Stanton B. Gelvin | 1996 | Plant Molecular Biology1996，，1： | 1 |
| 7 | Subcellular distribution and tissue expression ofphospholipase Dα,Dβ and Dγ in Arabidopsis显示文摘 | Fan Lu Zheng Suqin Cui Decai | 1999 | Plant Physiology1999，119，： | 1 |
| 8 | Health Assessment and Protection Countermeasures of Ecosystem of the Dajiu Lake in Shennongjia显示文摘The Dajiu Lake is an important subtropical alpine wetland in central China.In May of 2019,an ecological investigation was conducted on water quality and aquatic organisms(phytoplankton,zooplankton and zoobenthos)of the Dajiu Lake in Shennongjia forest region.The results showed that parameters of water quality in No.5 and No.9 sub lakes of the Dajiu Lake corresponded with standard of class II water of the Environmental Quality Standards for Surface Water(GB 3838-2002),while other sub lakes corresponded with standard of class III water.Diversity indexes of phytoplankton and zooplankton ranged from 2.0 to 3.0,and there were two families(Chironomidae and Libellulidae Rambur)of one phylum(Arthropoda)in zoobenthos.Comprehensive health index of entropy weight was used to evaluate water ecological status of the Dajiu Lake,and water quality of No.5,No.8 and No.9 sub lakes was in a healthy status,while other sub lakes were in sub-health status.Finally,corresponding countermeasures were proposed according to protection needs of the Dajiu Lake. | Decai HE Xiaobin PAN Ying CUI Yanyan WANG | 2021 | Meteorological and Environmental Research2021，12，3： | 0 |
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